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Sulfate-resistant Rhodococcus erythropolis and use thereof

A technology of Rhodococcus erythropolis and strains, applied in the biological field, can solve the problems of low desulfurization activity and narrow substrate range

Active Publication Date: 2005-11-23
CHINA PETROLEUM & CHEM CORP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, the above-mentioned bacteria and their practical applications in desulfurization have disadvantages such as: low desulfurization activity, desulfurization activity is inhibited by product sulfate, and the range of substrates that the strains can act on is narrow.
However, there are no reports on the application of Rhodococcus erythropolis with sulfate tolerance and broad substrate range in the removal of organic sulfur in fossil fuels.

Method used

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  • Sulfate-resistant Rhodococcus erythropolis and use thereof
  • Sulfate-resistant Rhodococcus erythropolis and use thereof
  • Sulfate-resistant Rhodococcus erythropolis and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Example 1: Screening of Rhodococcus erythropolis SDUZAWQ strain

[0057] Strain screening Obtain soil samples and water samples from places such as refinery sewage outlets, use basic inorganic salt medium (medium composition: 1 liter of distilled water contains: 2.44g of KH 2 PO 4 , 5.57 g Na 2 HPO 4 , 2g NH 4 Cl, 0.2 g MgCl 2 , 2g of glycerin, 1mL of trace element mixture. The composition of the trace element mixture is: 1 liter of distilled water contains: 1g of CuCl 2 2H 2 O, 4 g CoCl 2 ·6H 2 O, 2g ZnCl 2 , 40g CaCl 2 , 0.5 g H 3 BO 3 , 2 g NaMoO 4 2H 2 O, 40g FeCl 3 ·7H 2 O, 1 g AlCl 3 ·6H 2 O, 8g MnCl 2 4H 2 O) Soak overnight, culture at 30° C. with shaking at 200 rpm for 48 hours. The culture medium was centrifuged to remove the soil sample, and then the bacteria were obtained by centrifugation. The bacteria were washed twice with 0.85% physiological saline, and suspended in the pH7.0 potassium phosphate buffer. Add 1 mL of this bacterial sol...

Embodiment 2

[0058] Embodiment 2: Preparation of cell liquid culture of Rhodococcus erythropolis SDUZAWQ bacterial strain:

[0059]Pick a nutrient agar slant to cultivate a ring of Rhodococcus erythropolis SDUZAWQ strain and inoculate it in a 300ml Erlenmeyer flask containing 50ml of sterilized basic inorganic salt medium, and add sterilized DBT-dimethylformamide stock solution , the DBT concentration was 0.5mM, and cultured on a shaker at 30°C and 180 rpm for 36-48h. Pipette 5ml of the cultured bacterial solution and inoculate it into a 500ml Erlenmeyer flask filled with 100ml of basic inorganic salt medium, and then add sterilized DBT-dimethylformamide stock solution to make the DBT concentration 0.1-0.5mM. The Erlenmeyer flask was placed in a shaker and cultured at 30° C. and 180 rpm for 48 hours to prepare a liquid culture solution of Rhodococcus erythropolis SDUZAWQ cells.

Embodiment 3

[0060] Embodiment 3: Preparation of Rhodococcus erythropolis SDUZAWQ strain resting cell liquid:

[0061] The liquid culture solution of the cells of the strain SDUZAWQ obtained by the method of Example 2 was centrifuged at 5000 rpm for 10 min, and the supernatant was poured off. Wash and centrifuge three times with an appropriate amount of normal saline, suspend the obtained centrifuged sediment in normal saline, and then freeze and store in a refrigerator to obtain resting cells of the Rhodococcuserythropolis SDUZAWQ strain.

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Abstract

The Rhodococcus erythropolis related by the invention is Rhodococcus erythropolis SDUZAWQ, which is stored in 'China microorganism strain preservation management committee common micro organism center' at March 1st, 2004, and whose storage number is CGMCC NO. 1922. The strain is obtained by separating from sewage water from oil-extraction plant or soil. The cell culture fluid of the strain can high effectively remove sulfureous in dibenzothiophene, 4, 6-dimethyl-dibenzothiophene, 4-methyl-dibenzothiophene, benzothiophene, 5-methyl-benzothiophene by oxidative scission of C-S bond. Mixed sulfureous source of dibenzothiophene and sodium sulfate is used for culturing the Rhodococcus erythropolis SDUZAWQ, and the obtained resting cell culture fluid can effectively remove the sulfureous contained in diesel oil which has been subject to hydrofining.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a sulfate-tolerant Rhodococcus erythropolis and its application in removing organic sulfur from fossil fuels. Background technique [0002] The organic and inorganic sulfur contained in fossil fuel coal and petroleum is an important source of environmental pollution. When these sulfides are burned, they emit a large amount of toxic gases such as sulfur dioxide, which causes large areas of acid rain to seriously pollute the air and water sources, and destroy the ecological balance. endanger human existence. Years of continuous development have resulted in fewer and fewer low-sulfur fossil fuels in existence. With the gradual intensification of the energy crisis, the exploitation of high-sulfur fossil fuels has become inevitable. High sulfur fossil fuels must be desulfurized before further use. The cost of physical and chemical desulfurization methods is huge, while the microbial de...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 许平马翠卿李福利曾义勇佟明友方向晨张全
Owner CHINA PETROLEUM & CHEM CORP
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