Carbendazol composite degrading bacteria and method for degrading carbendazol

A technology for carbendazim, degrading bacteria, used in microorganism-based methods, biochemical equipment and methods, bacteria, etc.

Inactive Publication Date: 2011-02-16
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the application of chemical pesticides in agricultural production cannot be completely replaced. Therefore, how to effectively remove the pollution caused by chemical pesticides has attracted widespread attention.

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  • Carbendazol composite degrading bacteria and method for degrading carbendazol
  • Carbendazol composite degrading bacteria and method for degrading carbendazol
  • Carbendazol composite degrading bacteria and method for degrading carbendazol

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Embodiment Construction

[0012] The present invention will be described in detail below in conjunction with specific embodiments.

[0013] Medium: ① LB liquid medium: NaCl 10.0g, peptone 10.0g, yeast extract powder 5.0g, distilled water 1000mL, pH 7.0. ② Inorganic salt medium: NaCl 1.0g, K 2 HPO 4 1.5g, KH 2 PO 4 0.5g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 ·7H 2 O 0.2g, distilled water 1000mL, pH 7.0.

[0014] Reagents and instruments: carbendazim (provided by Shaanxi Petrochemical Research Institute, with a purity of 98%); UV-1200 ultraviolet-visible spectrophotometer, purchased from Shanghai Meipuda Instrument Co., Ltd.; SKY-200B constant temperature culture oscillator purchased From Shanghai Sukun Industrial Co., Ltd.; high performance liquid chromatography LC-2010AHT, purchased from Japan Shimadzu Corporation.

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Abstract

The invention discloses carbendazol composite degrading bacteria and a method for degrading carbendazol. The carbendazol composite degrading bacteria consist of alcaligenes M-1 and rhodococcus erythropolis L-1 in a compound ratio of 1:4. In the method for degrading the carbendazol, the inoculum concentration is 7 percent; the pH of the degrading condition is 6; the temperature is 30 DEG C; and the degrading time is 24 hours. The degrading rate of the carbendazol is up to 75.76 percent in 24 hours.

Description

technical field [0001] The invention relates to a carbendazim compound degrading bacteria and a method for degrading carbendazim, which belong to the field of biological high-tech, and use microorganisms to degrade chemical pesticide residues, and are suitable for bioremediation of carbendazim-contaminated soil and in modern agriculture. Production of green and pollution-free agricultural products. Background technique [0002] In agricultural production, people widely use various organic pesticides such as insecticides, fungicides, acaricides and herbicides. Among them, carbendazim is a type of fungicide with a demand of 10,000 tons, and it is the main type of pesticide exported in my country. Since it was developed by American DuPont Company in 1967, carbendazim has been widely used in the anti-mold and anti-corrosion of crop diseases caused by fungi and industrial materials. However, the chemical properties of carbendazim are stable, and its degradation half-life is lon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D101/04C12R1/05A62D101/28C12N1/20C12N1/00B09C1/10C12R1/01A62D3/02
Inventor 呼世斌朱凤晓冯贵颖王军玲许炳云孔洁
Owner NORTHWEST A & F UNIV
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