33 results about "O-Phthalaldehyde" patented technology

The invention relates to the technical field of polypeptide cyclization methods, in particular to a preparation method for constructing a phage display cyclopeptide library based on phthalic dicarboxaldehyde, which comprises the following steps: carrying out click chemical reaction on phthalic dicarboxaldehyde (OPA) and lysine and cysteine in an unprotected peptide sequence under a neutral or alkaline condition to form cyclopeptide; constructing a phage cyclic peptide library on the basis of the reaction property that the OPA molecule is efficiently biocompatible with sulfydryl and amino groups; a polypeptide library containing cysteine and lysine is displayed on a bacteriophage, the polypeptide library is cyclized by utilizing OPA (o-phthalaldehyde), and a cyclic peptide ligand with specific binding is screened out aiming at a target protein. In the strategy of performing polypeptide cyclization by using o-phthalaldehyde, it is found that o-phthalaldehyde has good reaction activity with sequence peptide containing cysteine and lysine, reaction conditions are simple and mild, and the o-phthalaldehyde has good biocompatibility. The reaction system does not need organic solvents or metal catalysts and is suitable for biological systems.

The invention discloses o-phthalaldehyde disinfectant concentration detection test paper and a preparation method thereof.The preparation method comprises the steps that S1, a color developing agent, a color developing accelerant and a stabilizer are dissolved in purified water, a solution A is prepared, and the color developing agent accounts for 0.1-10 wt%, the color developing accelerant accounts for 0.1-10 wt%, and the stabilizer accounts for 0.1-10 wt%; s2, the filter paper is completely soaked in the liquid A for 1-2 min, and the filter paper is dried after being completely soaked; s3, uniformly dissolving a film-forming agent in purified water to prepare 0.1-10wt% of a solution B; s4, immersing the filter paper dried in the step S2 into a solution B, continuing for 10-30s, and then drying; and S5, cutting the dried filter paper, and pasting the cut filter paper on a substrate paperboard to obtain the phthalic dicarboxaldehyde disinfectant concentration detection test paper. The test paper for detecting the concentration of the o-phthalaldehyde disinfectant has the advantages that the validity period is as long as 2 years, the color development effect is stable, and the concentration of the o-phthalaldehyde disinfectant can be effectively and accurately reflected.

The invention relates to a production reaction device for preparing o-phthalaldehyde disinfectant in the field of stirring preparation equipment. When preparing the disinfectant, high-pressure airflow is used to compress and mix o-phthalaldehyde powder and diffuse and spray it out to generate atomized raw material powder and spray the atomized powder onto the inner wall of an atomizing mixing cylinder. At the same time, pure water is extracted by a submersible pump and sprayed onto the inner wall of the atomizing mixing cylinder through a spray cylinder, so that the inner wall of the atomizing mixing cylinder is wetted to generate a flowing water film, and atomized water droplets are generated by impact and rebound of a water column. With the rotation of the spray cylinder, the atomized powder is fully contacted and dissolved with the atomized water droplets and the flowing water film on the inner wall of the atomizing mixing cylinder, thereby improving the uniformity of contact between the o-phthalaldehyde raw material and the pure water, reducing the situation that powder agglomerates are generated when the raw material powder is concentratedly poured and contacts with the pure water, and reducing the time for dissolving and stirring.

The invention relates to the technical field of chemical analysis and detection, in particular to a fluorescence determination method for free amino acid in tobacco leaves, based on the fluorescence characteristic of epinephrine-based zinc-doped carbon dots Zn / NA-CDs, free amino acid reacts with phthalic dicarboxaldehyde to generate a Schiff base product, and Zn / NA-CDs and Schiff base have hydrogen bond interaction and charge transfer effect, so that the content of free amino acid in tobacco leaves is determined. Therefore, the Schiff base has a fluorescence sensitization effect on the Zn / NA-CDs, and a free amino acid fluorescence detection method is established. According to the method, the detection limit can reach 0.01 mg / L, and the recovery rate is between 90.52% and 105.33%, so that the method also has high sensitivity to low-concentration free amino acid, can meet the requirement for accurately measuring trace amino acid in tobacco leaves, and has excellent accuracy and reliability. Meanwhile, the method further has the advantages of being easy and convenient to operate, low in cost, environmentally friendly, wide in applicability and the like, a new technical means is provided for tobacco leaf quality detection, and beneficial reference is provided for amino acid determination in other fields.

The invention discloses a preparation method of silicon dioxide composite thermal insulation aerogel. According to the invention, o-phthalaldehyde is used as a cross-linking agent to prepare the silicon dioxide composite thermal insulation aerogel with thermal insulation function, high skeleton strength and good adsorption capacity, and on the basis, sodium lauroyl glutamate is used as an air template to prepare the new silicon dioxide composite thermal insulation aerogel. On the premise that the whole gelation process is not damaged, the pore volume of the aerogel is increased, the separation effect in oil-water separation is further improved, the excellent flame-retardant capability is shown, and finally, the flame-retardant aerogel can be used for preparing oil-water separation oil-water separation oil-water separation oil-water separation oil-water separation oil-water separation oil-water separation oil-water separation. Chitosan is selected to be subjected to quaternization modification and then is directly compounded with hydrophobized silicon dioxide aerogel powder to obtain the improved silicon dioxide composite heat preservation type aerogel, the improved silicon dioxide composite heat preservation type aerogel not only has a more complex and slender framework structure, but also increases the overall surface area and surface roughness of the gel, and the adsorption capacity of the aerogel is further enhanced.

The invention relates to a diyne functionalized covalent organic framework material as well as a preparation method and application thereof, and the diyne functionalized covalent organic framework material is synthesized by taking 4, 4 ', 4', 4 ''-(pyrene-1, 3, 6, 8-tetrayl) tetraphenylamine and 5, 5 '-(butyl-1, 3-diyne-1, 4-diyl) diisophthalaldehyde as construction units and adopting a Schiff base reaction. The dialkynyl functionalized covalent organic framework material disclosed by the invention has good chemical stability and can be used as a carrier to controllably grow silver nanoparticles through metal coordination to obtain a composite material, and the composite material shows excellent catalytic activity and cycling stability in a 4-nitrophenol reduction reaction catalyzed by sodium borohydride; therefore, the method has important application potential in the fields of environmental restoration, green chemistry and the like.

The invention relates to a test paper for detecting concentration of o-phthalaldehyde disinfectant in the field of detection technology. The test paper comprises a bottom layer made of a hydrophobic material; a microfluidic layer arranged on the bottom layer, and a plurality of functional areas are divided by hydrophobic boundaries; an intelligent response detection layer arranged on the microfluidic layer, and comprising temperature-responsive hydrogel microspheres and a signal enhancer; a self-calibration layer arranged on the microfluidic layer; a sample input area at one end of the test paper, and provided with a water absorption layer; and a color-variable indicating element arranged above the intelligent response detection layer, the self-calibration layer and an integrated colorimetric card. When in use, a sample is added to the sample input area and activated with ultraviolet light. After the reaction is completed, a result can be obtained by visual comparison or scanning with an intelligent device. The invention has the advantages of high sensitivity, strong specificity, simple operation, rapid detection, accurate results, and the like, and is suitable for rapid detection of o-phthalaldehyde concentration in multiple fields such as medical care, food safety, and environmental monitoring.

The invention belongs to the technical field of disinfectants and discloses a high-level disinfectant and a preparation method thereof.The high-level disinfectant is prepared from, by mass, 0.1%-3% of phthalic dicarboxaldehyde, 0.2%-0.8% of chelating agent, 2%-5% of penetrating agent, 0.3%-2% of modified starch, 1%-2% of acrylic polymer, 0.1%-0.3% of single-chain quaternary ammonium salt, 0.1%-0.3% of double-chain quaternary ammonium salt, 0.4%-5% of corrosion inhibitor and the balance purified water. Modified starch and polyacrylic acid are fully dissolved in a high-temperature stage, molecular chains are fully expanded, more active sites are exposed, and then the temperature is gradually reduced, so that the molecular chains are orderly arranged, a compact and uniform network structure is formed, and effective release of bactericidal components is ensured. Meanwhile, the single-chain quaternary ammonium salt and the double-chain quaternary ammonium salt are complementary through membrane destruction and internal destruction respectively and have a synergistic effect with the o-phthalaldehyde, so that the sterilization effect is remarkably improved.

A method for measuring the concentration of phthalic dicarboxaldehyde (OPA) in a sample is provided. The method includes measuring the absorbance of the OPA at a specific wavelength using a spectrophotometer, and converting the absorbance value as a function of temperature (slope) into a concentration value using a standard curve. The methods are specific (selective), simple, rapid, and highly accurate, making them suitable for use in various applications, such as monitoring OPA concentrations in disinfection / sterilization industrial environments.

The invention relates to the technical field of o-phthalaldehyde disinfectants, and discloses o-phthalaldehyde disinfectant preparation equipment and a preparation method thereof.The o-phthalaldehyde disinfectant preparation equipment comprises a base, a processing barrel fixedly connected to the top end of the base, a liquid drainage pipe fixedly connected to the bottom end of the processing barrel in a penetrating mode, and a water injection pipe fixedly connected to the side wall of the processing barrel in a penetrating mode. A gear is driven by a toothed plate to intermittently rotate, so that the gear controls a baffle through a special-shaped plate to intermittently seal an atomizing mixing barrel, and a mixed solution in the atomizing mixing barrel intermittently flows into a connecting barrel; the problem that the solution in the atomization mixing cylinder cannot flow completely in the process of flowing into the connecting cylinder at a time is avoided, meanwhile, the content of the mixed solution flowing into the connecting cylinder every time is controlled through the concentration detection controller, and then the concentration of the phthalic dicarboxaldehyde disinfectant can be controlled according to needs; furthermore, the flexibility of preparing the phthalic dicarboxaldehyde disinfectant by the equipment is improved.

This invention relates to a method for preparing a β-cyclodextrin functionalized covalent organic framework composite material. The method involves an aldehyde-imine condensation reaction between the aldehyde groups on 2,3,4-trihydroxybenzaldehyde and p-phthalaldehyde and the amino group on hepta(6-amino-6-deoxy)betacyclodextrin to construct a β-CD-COF layer. This one-step method produces a β-cyclodextrin covalent organic framework composite material that exhibits excellent enrichment capacity for phosphopeptides, facilitating purification and extraction. It also shows promising application prospects in mass spectrometry detection and exosome separation.

A method for measuring the concentration of ortho-phthalaldehyde (OPA) in a sample is provided. The method involves the use of a spectrophotometer to measure the absorbance of OPA at a specific wavelength, and the use of a standard curve to convert the change in absorbance value with temperature (slope) to a concentration value. The method is specific (selective), simple, rapid, and highly accurate, making it suitable for use in a variety of applications, such as the monitoring of OPA concentrations in disinfection / sterilization industrial settings.

The invention relates to an o-phthalaldehyde disinfectant. The o-phthalaldehyde disinfectant comprises the following components in percentage by weight: 0.1-1 wt% of o-phthalaldehyde; 10 to 30 weight percent of polybasic acid salt; 0.01 to 0.05 wt% of an antioxidant; 0.005 to 0.05 wt% of a polymerization inhibitor; 0.1 to 0.5 wt% of a slow release agent; and the balance of water. The o-phthalaldehyde disinfectant disclosed by the invention can effectively prevent grey dyeing, is short in sterilization time and is suitable for high-level disinfection and sterilization of medical instruments.

The invention discloses phthalic dicarboxaldehyde concentration detection test paper as well as a preparation method and application thereof, and belongs to the technical field of chemical detection. The preparation method comprises the following steps: S1, dissolving a salt compound formed by sulfite, organic amine and acid into water to obtain a soaking solution; s2, immersing filter paper into the soaking solution, taking out the filter paper after full soaking, and drying the filter paper; and S3, pasting the dried filter paper on a non-absorbent substrate to obtain the phthalic dicarboxaldehyde concentration detection test paper: taking a certain volume of phthalic dicarboxaldehyde solution and dropwise adding the phthalic dicarboxaldehyde solution on the test paper during use, and observing the color change of the test paper after a period of time to judge whether the concentration of the phthalic dicarboxaldehyde solution is in an effective range or not. The method disclosed by the invention is simple, the concentration of the effective components is not easy to change in the drying process and the storage process, and the prepared test paper can be used for rapidly and accurately detecting the concentration of the phthalic dicarboxaldehyde.

The application discloses a synthesis method of o-phthalaldehyde. O-xylene, a halogenated reagent and an organic solvent are mixed, and under ultraviolet light irradiation, a bromination product with different bromination degrees is generated by reaction; water is added into the reaction liquid, and the reaction is continuously carried out; after the reaction is completed, the organic solvent and water are removed by evaporation, and a crude ɑ, ɑ, ɑ', ɑ'-tetra-brominated o-xylene product is obtained; the crude product is recrystallized to obtain ɑ, ɑ, ɑ', ɑ'-tetra-brominated o-xylene product; and the ɑ, ɑ, ɑ', ɑ'-tetra-brominated o-xylene is hydrolyzed to obtain o-phthalaldehyde. The synthesis method optimizes the bromination and hydrolysis process, and by adding appropriate water in the o-xylene bromination process, the yield of the final product o-phthalaldehyde is improved.

The application discloses a kind of meat freshness rapid test paper, belong to food rapid detection technical field.The test paper is with water absorption filter paper as base material, and is loaded by the reaction color developing system consisting of o-Phthaldehyde, mercapto glycolic acid, sodium acetate buffer and fast blue B salt, can occur specific color reaction with the biological amine (putrescine) generated in the meat spoilage process, and realizes freshness rapid judgment by color ladder change.The detection time of the application is only 3-5 minutes, without large instrument, simple operation, high sensitivity, low cost, wide spectrum, can be used for on-site rapid screening of pork, beef, mutton, frozen shrimp, frozen chicken breast and other various meats, and is widely applicable to raw material acceptance of meat processing enterprises, market supervision law enforcement and cold chain logistics quality monitoring.

The present invention relates to disinfection technology, and particularly to a quality control technology for o-phthalaldehyde disinfectant solution. A rapid detection indicator card impregnation composition for o-phthalaldehyde disinfectant solution, which comprises the following in mass percentages: stabilizer: 1% - 10%; chelating agent: 5% - 20%; neutralizing agent: 0.1% - 2%; chromogenic agent: 0.1% - 20%; chromogenic accelerator: 1% - 4%; the balance is pure water. After impregnating filter paper with the composition and drying it, it is pasted on white plastic paper with double-sided tape and compacted to form the rapid detection indicator card. The indicator card of the present invention has more stable, obvious and clear color development, rapid detection and more reliable results.

Methods of constructing conjugates are disclosed. The preparation method comprises the following steps: reacting an amino-containing component and a thiol-containing component with o-phthalaldehyde in one step under a considerable molar ratio to generate a conjugate with a substituted or unsubstituted 1-thio-isoindole structure in the presence of an additive. The method provides an efficient and practical platform for constructing the biological activity modular primitive.

The invention discloses a post-column derivatization detection method of ethyl lauroyl arginate, which comprises the following steps: pre-treating food, extracting ethyl lauroyl arginate hydrochloride with an acetonitrile aqueous solution, filtering with an aqueous filter membrane, separating with a high performance liquid chromatography column, taking mercaptan and o-phthalaldehyde as derivatization reagents, and carrying out post-column derivatization detection on ethyl lauroyl arginate under an alkaline condition, so as to obtain the ethyl lauroyl arginate. And carrying out a derivative reaction to generate a fluorescent derivative, carrying out derivative fluorescence detection, and quantitatively determining the content of the ethyl lauroyl arginate hydrochloride. The method has the characteristics of rapidness, high sensitivity and low detection limit. The method is suitable for determination of trace ethyl lauroyl arginate hydrochloride in dairy products, fruit products, meat products, aquatic products, beverages or gum-based candies. And a detection method is provided for the detection of trace ethyl cinnamyl arginate hydrochloride.

The present invention belongs to the field of macromolecular and polymer synthesis. Aiming at the problems that the polymerization reaction of polyphthalaldehyde (POPD) generated by polycondensation of POPD monomer usually needs to be carried out at an ultra-low temperature (≤-40°C), the polymer has poor stability at room temperature and is easy to depolymerize, and the polymer needs to be end-capped after the reaction, the present invention provides a single-component organic Lewis acid-base pair having the structural formula: #imgabs0#n is the carbon chain length, R is any one of hydrogen, methyl, ethyl, butyl, phenyl or substituted phenyl; X is any one of halogen Cl, Br, I, acetate, trifluoroacetate, and succinate; R 1 、R 2 It is one or a combination of hydrogen, alkyl, cycloalkyl, aryl or substituted aryl; Y is a bridged skeleton. The single-component organic Lewis acid-base pair can achieve polymerization of o-phthalaldehyde and epoxy compounds under relatively mild conditions, and the obtained polymer does not need to be end-capped and can be purified for use.

The invention discloses an analysis method for precolumn derivatization of a hydrogenation impurity of a melogabalin besylate intermediate, the melogabalin besylate intermediate is MB-2, and the hydrogenation impurity in the melogabalin besylate intermediate is MB-2A, the analysis method comprises the following steps: (1) using a derivatization reagent to perform precolumn derivatization on the melogabalin besylate intermediate; carrying out derivative reaction on the melogabalin besylate intermediate under the catalysis of alkali to obtain derivative reaction liquid; the derivatization reagent is o-phthalaldehyde, fluorenyl methyl chloroformate or 2, 4-dinitrofluorobenzene; and (2) detecting the derivative reaction liquid by using a high performance liquid chromatography-UV detector to obtain the content of the hydrogenated impurities in the melogabalin besylate intermediate. According to the analysis method, the content of MB-2A in MB-2 can be effectively separated and accurately quantified, the accuracy is high, the detection line is low, and the method is high in durability. # imgabs0 #

The invention belongs to the field of protein hydrogel materials, and particularly relates to elastin-like and a preparation method and application of elastin-like hydrogel. According to the present invention, the two elastin-like proteins A and B having different amino acid sequences are utilized to form the cross-linked network through the isopeptide bond, such that the mechanical strength of the polymer hydrogel composed of the four-arm polyethylene glycol amine, 4-arm PEG-NH2 and the o-phthalaldehyde modified four-arm polyethylene glycol, 4-arm PEG-OPA can be effectively improved, the adhesion can be improved, and the cross-linked network can be used for the preparation of the polymer hydrogel, such that the cross-linked network can be formed through the cross-linked network, such that the cross-linked network can be used for the preparation of the polymer hydrogel, and the use amount of the polymer hydrogel can be reduced. The obtained elastin-like hydrogel has the advantages of strong wet tissue adhesion capacity, good biocompatibility, injectable use and the like, and is beneficial to application in the biomedical fields of hemostasis, tissue repair and the like.

This invention discloses an amino acid assay kit based on the o-phthalaldehyde (OPA) method, its preparation method, and its application. Belonging to the field of amino acid detection and analysis technology, it is used for rapid and accurate quantitative detection of amino acids in samples. The amino acid assay kit can not only measure the total amino acid content but also analyze specific amino acids and dipeptides such as alanine glutamine, meeting diverse analytical needs. This amino acid assay kit is suitable for batch testing and supports high-throughput laboratory applications. By optimizing the reagent formulation and operating procedures, this invention effectively simplifies the detection steps, improves detection sensitivity and specificity, and fills the gap in the market for OPA-based assay kits. This invention can be widely applied in nutrition, medicine, clinical diagnostics, and drug development, providing a convenient and efficient amino acid analysis tool for life science and medical research.

The invention discloses a method for detecting an impurity E in a granisetron hydrochloride injection by utilizing an HPLC (High Performance Liquid Chromatography) on-line derivatization method, belonging to the technical field of chemical engineering and pharmacy. According to the method, a high performance liquid chromatography method is adopted, an Ultimate AQ-C18 column is used as a chromatographic column, a mixed solution of a 0.1% phosphoric acid solution and acetonitrile is used as a mobile phase, and an o-phthalaldehyde solution is used as a derivatization reagent; quantitative detection of the impurity E in the granisetron hydrochloride injection is realized through specific instrument conditions, solution preparation and measurement recording steps. The method is simple and convenient to operate, strong in specificity, high in sensitivity and accuracy, good in linearity and capable of effectively detecting the quality of the granisetron hydrochloride injection product.

The present application relates to a kind of diacetylene functionalized covalent organic framework material and its preparation method and application, to 4,4',4'',4'''-(pyrene-1,3,6,8-tetrayl) tetraphenylamine, 5,5'- (but-1,3-diethynyl-1,4-diyl) diisophthalaldehyde as building unit, synthesis using Schiff base reaction.The diacetylene functionalized covalent organic framework material of the present application has good chemical stability, and can be used as carrier by metal coordination effect Controllable growth silver nanoparticles to obtain composite material, the composite material in sodium borohydride catalytic 4-nitrophenol reduction reaction has shown excellent catalytic activity and cycle stability, thus has important application potential in the field such as environmental remediation and green chemistry.

The application discloses a method for preparing o-phthalaldehyde from o-xylene. V2O5-TiO2 doped with metal elements is used as a catalyst, o-xylene and an oxygen-containing gas are reacted to obtain a mixed oxidation product containing o-phthalic anhydride, o-phthalic acid and phthalide; the mixed oxidation product is reacted with a reducing agent in a solvent, and o-phthalalcohol is obtained after separation; and the o-phthalalcohol is reacted with an oxidizing agent, and o-phthalaldehyde is obtained after separation. The application avoids the use of corrosive substances such as bromine or chlorine and nitric acid, has low requirements on equipment, is small in environmental pollution, and is high in overall yield of o-phthalaldehyde.

The invention belongs to the technical field of medical carriers, and relates to a hardness-controllable polyethylene glycol nano-drug carrier as well as a preparation method and application thereof. Adsorbing a layer of polyethyleneimine on the surface of the template to obtain a carrier precursor; performing at least one layer assembly on the surface of the carrier precursor; and finally, removing the template. The primary layer assembly process comprises the following steps: firstly, adding multi-arm polyethylene glycol o-phthalaldehyde to carry out a cross-linking reaction, and then adding multi-arm polyethylene glycol amino to carry out a cross-linking reaction; the cross-linking reaction is that the o-phthalaldehyde group reacts with the primary amino group to generate the isoindolinone group. The PEG nano-drug carriers with different hardness can be prepared through a layer-by-layer assembly method, and targeting molecules and / or chemotherapeutic drugs can be introduced in the preparation process; by adjusting the PEG nano-drug carrier with proper hardness and loading the chemotherapeutic drug, the interaction between the nano-drug carrier and a biological interface can be adjusted, so that good tumor treatment is realized.

The present invention proposes a preparation method of an o-phthalaldehyde disinfectant with a concentration indication function, comprising the following steps: S1, preparing a buffer solution: mixing a phosphate solution with a strong alkaline solution, diluting and shaking; S2, preparing a color developer: dissolving ninhydrin with n-butanol, diluting with water, adding thymol blue, shaking and standing; S3, adding the prepared buffer solution and color developer to the disinfectant stock solution, shaking and standing; S4, adding sodium sulfite to the disinfectant prepared in step S3, adjusting the effective content concentration, standing, and observing the color change. The o-phthalaldehyde disinfectant prepared by the present invention has a concentration indication function, and can change color according to the concentration change of its effective ingredient, so as to assist the user to judge whether the disinfectant can continue to be used more quickly, intuitively and accurately; at the same time, it can also prevent the disinfectant contamination problem caused by the frequent use of detection tools, as well as the cost problem of detection tool consumables.

The invention discloses a pathogenic nucleic acid extraction method based on reverse nucleic acid grabbing, which comprises the following steps: adding a sephadex microsphere solution with the surface modified with functional molecules and a lysis buffer into a bacterial liquid containing pathogenic bacteria, oscillating, inserting filter paper with negative electricity, incubating, taking out the filter paper, centrifuging, and taking out supernatant to obtain purified pathogenic nucleic acid, the functional molecules are o-phthalaldehyde, phenylboronic acid and hydrophobic molecules, the lysate contains lauryl sodium sulfate, disodium hydrogen phosphate and ethylenediamine tetraacetic acid, and the pH value of the lysate is 10.0-10.5. The method has the advantages of simplicity and convenience in operation, high extraction speed, high nucleic acid quality, high nucleic acid extraction rate and the like, and can provide a high-quality nucleic acid sample for molecular diagnosis.

Disclosed are methods for constructing conjugates. An amine-containing component and a thiol-containing component, in comparable mole ratios, react with an ortho-phthalaldehyde in one step, to generate a conjugate bearing a substituted or an unsubstituted 1-thio-isoindole structure in the presence of an additive. The method provides a platform for the efficient and practical constructions of bioactive modular motifs.