136 results about "O-Phthalaldehyde" patented technology

Antimicrobial lubricant compositions are disclosed. The antimicrobial lubricant compositions are particularly useful in providing antimicrobial capability to a wide-range of medical devices. The compositions include an oil lubricant. Representative lubricants may include polydimethyl siloxane, trifluoropropyl copolymer polysiloxane, and a copolymer of dimethylsiloxane and trifluoropropylmethylsiloxane. The compositions include rheology modifiers as necessary. The compositions also include antimicrobial agents, which may be selected from a wide array of agents. Representative antimicrobial agents include of aldehydes, anilides, biguanides, bis-phenols, quaternary ammonium compounds, cetyl pyridium chloride, cetrimide, alexidine, chlorhexidine diacetate, benzalkonium chloride, and o-phthalaldehyde.

The invention provides a disinfectant with low corrosiveness. The disinfectant comprises the following components by weight percent: 0.2-2.0% of ortho-phthalaldehyde, 1-10% of a synergist, 10-50% of a cosolvent, 0.5-5% of a buffering agent, 1-10% of a penetrant, 0.2-2% of a corrosion inhibitor, 0.1-5% of a chelating agent, 0.1-3% of a stabilizing agent and 45-80% of purified water. After the disinfectant is used, intestinal tract pathogenic bacteria, pyogenic coccus, hepatitis virus, H7N9 avian influenza virus, black variant spores of bacillus subtilis and other pathogenic microorganisms can be killed within a short time. Furthermore, the disinfectant adopts a slow release technique, thus having extremely low corrosiveness on metal and being very suitable for disinfecting medical equipment.

The invention relates to a detection method for gamma-aminobutyric acid (GABA) content in food by using high performance liquid chromatography (HPLC) method. The method is characterized by: carrying out sufficient whirling through double distilled water, and an ultrasonic extraction for a sample requiring detection, then carrying out a centrifugating to obtain supernatant, followed by filtering the supernatant to obtain the sample liquid; weighing the GABA standard substance, then dissolving the GABA through the double distilled water, and carrying out a metered volume for the GABA solution to adopt the solution as a standard stock solution, further dissolving the standard stock solution through the double distilled water to obtain a standard working solution having a concentration gradient; adopting o-phthalaldehyde and 2-mercaptoethanol as derivating agents, and carrying out a derivation for the sample liquid through a HPLC equiped with a online derivation apparatus, followed by carrying out a quantitative detection through a external standard method. The method for detecting the GABA content in the food is quick and effective, and has a linearity range of 0.5-1000 mg/L, a recovery rate more than 88.2%, a relatibe standard deviation (RSD) less than 5%. In addition, with the present invention, a reliable and convenient method is provided for detecting the GABA content in the food,; requirements of researches and productions are met.

The invention discloses a test method of liquid spectrum finger diagram of melon, or melon injection, comprising that (1), preparing melon, or melon injection into a sample solution, (2), respectively absorbing citrulline water solution and the sample solution, to be filled into a liquid spectrometer, and using the solution of phthalaldehyde and the solution of chloride aminic acid fluorenes methyl ester to process derivatization, (3), testing liquid spetrum. The inventive test method first discloses a liquid spectrum finger diagram of melon or melon injection, with high accuracy, stability and repeatability, as one analysis test method which can control the product quality, used in product quality test. The inventive method first obtains the high-effect liquid spectrum contrast finger diagram of melon and melon injection, as diagram 1 and diagram 2.

The invention relates to a method for qualitatively and quantitatively analyzing free amino acids in a sample by utilizing reversed phase high performance liquid chromatography, which comprises the following steps of: first pre-treating the sample; then protecting the -SH of cysteine in the sample; next performing pre-column derivatization treatment on primary and secondary amino acids with derivatization agents orthophaladetyde (OPA) and fluorene methyl choroformate (FMOC) respectively; and finally performing reversed phase high performance liquid chromatographic (RP-HPLC) analysis. The method has the advantage of synchronously detecting 25 free amino acids in the sample solution, particularly performing -SH protection on the cysteine with a dithio-dipropionic acid (DTDPA) and then synchronously deriving the cysteine together with the other amino acids, along with detection diversity and high efficiency.

A method and kit is provided for assaying homocysteine in a biological sample containing homocysteine and cysteine. A competing compound with an amino group (—NH₂) is mixed with the biological sample. An aldehyde, e.g. o-phthalaldehyde, is added to the biological sample to form homocysteine complex with fluorescence. The concentration of homocysteine in the biological sample is determined according to the fluorescent intensity.

The invention discloses a compound disinfectant for livestock and poultry raising environment and mainly comprises o-phthalaldehyde and deka-methonium bromide. The compound disinfectant comprises the components by weight percent: 0.05-1% of o-phthalaldehyde, 0.0001-0.05% of deka-methonium bromide, 1-5% of cosolvent, 0.001-0.1% of metal chelating agent, 0.001-1% of stable inhibitor and balance water; and the sum of the weight percent of the components in the scheme is 100%. By compounding the o-phthalaldehyde and the deka-methonium bromide, the compound disinfectant not only reduces the using quantity of the o-phthalaldehyde, the inconvenience for the field application caused by contamination and the irritation of disinfection for animals, but also avoids the disadvantage that the o-phthalaldehyde can not effectively kill bacterial spores. The compound disinfectant can be applied to air and ground sterilization of livestock and poultry for the animals in livestock and poultry houses, and air, ground, excrement and water sterilization of an animal farm. The compound disinfectant has the characteristics of efficiently killing environmental microorganism as well as low irritation, low corrosiveness and simple and convenient field application.

The invention relates to an o-phthalaldehyde disinfectant and a preparation method thereof. The o-phthalaldehyde disinfectant comprises the following components in percentage by weight: 0.2 to 1 percent of o-phthalaldehyde, 0.1 to 0.5 percent of salicylic acid, 0.5 to 1.5 percent of dodecyldimethylbenzylammonium chloride, 1 to 10 percent of ethanol, 0.1 to 1 percent of disodium hydrogen phosphate, 1 to 10 percent of ethanol, 0.1 to 1 percent of sodium dihydrogen phosphate, 0.1 to 0.5 percent of inhibitor, 0.01 to 0.1 percent of ethylene diamine tetraacetic acid and the balance of water. The method for preparing the disinfectant comprises the steps of: adding the o-phthalaldehyde into the ethanol according to the content and component, stirring and mixing the materials; adding water into the materials and stirring the mixture; and adding the dodecyldimethylbenzylammonium chloride, the disodium hydrogen phosphate, the sodium dihydrogen phosphate, the inhibitor and the ethylene diamine tetraacetic acid into the mixture in sequence and stirring the mixed solution to allow the added materials to dissolve to obtain the o-phthalaldehyde disinfectant. The disinfectant has the advantages of no pungent smell, safety, low toxicity, stable property and convenient use, can be used for high-level disinfection of the heat-labile medical equipment and is particularly applicable to an endoscope automatic washing and disinfecting machine to perform high-level disinfection on an endoscope.

The present invention discloses an OPA-NH4 <+> -SO3<2-> reaction-based sulfite ion spectroscopic measurement method which is as follows: an o-phthalaldehyde solution, an EDTA-NaOH buffer fluid and an ammonia nitrogen solution are successively added into a sulfite ion standard solution or a water sample, evenly shaken, placed at a certain temperature for reaction to equilibrium, and taken out and cooled to room temperature, spectrophotometer measuring wavelength is set for measuring solution absorbance A, the solution absorbance A is used as vertical coordinates, sulfite ion concentrations are used as x-coordinates for making a working curve; in a sample solution, sample solution absorbance As is measured according to the above method; and according to the working curve and the sample solution absorbance As, sulfite ion content in the sample solution can be quantified. The OPA-NH4 <+> -SO3<2-> reaction-based sulfite ion spectroscopic measurement method has the advantages of no need of use of toxic reagents, lower detection limits, higher sensitivity and the like.

The invention provides test paper for detecting the concentration range of an OPA (o-phthalaldehyde) disinfector. The test paper is manufactured from paper pulp subjected to oxidative modification, and the surface carboxyl group content of the paper pulp is controlled by adjusting modification conditions. The paper is processed step by step through a solution containing sodium sulfite and phenolphthalein, is dried and sheared, and is adhered to a baseplate to obtain the test paper. In a test, after contacting the disinfector for 90 seconds, the test paper becomes red uniformly, indicating that the concentration of OPA reaches a set value of the test paper, otherwise, the test paper does not change color uniformly or is colorless. According to the test paper, the surface carboxyl group content of fiber is controlled by modification of the paper pulp, and then a test card sensitive to different concentration ranges can be manufactured; color development is controlled by fiber modification and is more uniform and stable compared with color development obtained by controlling composition and concentration of a soak solution, thereby meeting the requirement of controlling the concentration of the OPA disinfector used for disinfecting medical instruments. The test paper is simple in operation, can ensure accurate results, and can be used for quickly and intuitively evaluating the disinfecting situation of the disinfector.

The invention relates to a preparation method of an allogeneic biological patch, and belongs to the technical field of biological material preparation. The preparation method comprises the steps of 1,obtaining people's skin tissues and a corium layer; 2, placing the corium layer in a fixing solution to perform fixing treatment; 3, placing the fixed corium layer in a decellularization solution toperform decellularization treatment to obtain decellularization corium tissues. The fixing solution is a phosphate buffer solution containing 1%-2% formaldehyde and 1.5%-3.0% o-phthalaldehyde; the decellularization solution is a 1.5-2.0 mol/L NaO or KOH alkaline solution containing 10-500 U/L trypsin, 1-25 mmol/L calcium chloride and 1-25 mmol/L magnesium chloride. The allogeneic biological patchhas effective fixing and supporting effects on breast implants, and different sizes are designed to satisfy the requirement of different patients and operating methods; in addition, because extremelylow immunogenicity and extremely excellent biocompatibility of the allogeneic biological patch, it is expected that occurrence rate of diolame contracture after the breast implants are implanted can be significantly reduced.

The invention discloses an OPA (ortho-phthalaldehyde)-containing compound disinfectant as well as a preparation method and an application thereof. The compound disinfectant comprises the following effective components: OPA, double-chain quaternary ammonium salt, a cosolvent, a metal chelator, dihydric phosphate, salt phosphate dibasic and water, and the pH of the compound disinfectant ranges from 7.5 to 9.0. According to the invention, the defect that single-component OPA cannot kill bacterial spores effectively is overcome through compounding of OPA and double-chain quaternary ammonium salt, and the application range of the OPA disinfectant is broadened. The disinfectant is suitable for high-level disinfection and sterilization of moisture-proof medical equipment which is not resistant to heat; and the compound disinfectant can be used for manual immersion disinfection and sterilization for the medical equipment and automatic mechanical flushing disinfection and sterilization, and has the characteristics of high efficiency (high-level disinfection through immersion for 20 min and sterilization for 45 min), no corrosion or pungent smell, safety, low toxicity, stable property, convenience in use and the like.

The invention relates to compound o-phthalaldehyde disinfection concentrated liquid, belonging to the technical field of disinfection. The disinfection concentrated liquid is prepared from the following components (by weight percent): 1-5% of o-phthalaldehyde, 2-15% of double-chain behenyl trimethyl ammonium chloride, 5-10% of a nonionic surfactant, 10-30% of fatty alcohol, 2-5% of a penetrating agent, 0.1-2% of a chelating agent, 0.5-3% of a corrosion inhibitor, 0.05-6% of organic acid and the balance being water. The disinfection concentrated liquid is broad in spectrum, efficient, low in toxicity, free of a pungent smell, stable in performance, low in corrosivity on metal, and especially applicable to disinfection of agricultural and breeding articles and environment, and can be diluted into 4,000 times to the maximal extent.

The invention discloses a formula of disinfection liquid for agricultural breeding. The disinfection liquid comprises the following components in parts by weight: 0.8 parts of o-phthalaldehyde, 4 parts of peroxyacetic acid, 0.9 parts of amino acid salt, 5 parts of active factors and 70 parts of purified water. The disinfection liquid is prepared by the following steps: fusing the components at a ratio, heating at a constant temperature of 65 DEG C for 5 minutes, naturally cooling, sub-packaging, checking, and warehousing. The formula of the disinfection liquid for agricultural breeding can overcome defects of low disinfection efficiency, poor safety, poor environmental protection property and the like in the prior art to achieve the advantages of high disinfection efficiency, good safety and good environmental protection property.

The invention discloses a phthalic dicarboxaldehyde-NH3-Na2SO3 reaction-based flow injection spectrophotometry method for measuring Ammonia-N in a water sample. The method specifically comprises the step of adopting a flow injection analyzer and an ultraviolet-visible spectrophotometer to jointly measure Ammonia-N content in a water sample. An amaranth complex is generated based on an OPA(O phthalic aldehyde)-NH3-Na2SO3 reaction; the complex realizes strong absorption at a 550nm position, and has an absorbance value in direct proportion to Ammonia-N concentration in the water sample, thus creating the flow injection spectrophotometry method for measuring Ammonia-N in the water sample. The detection limit of the method can be as low as 0.007mmol/L, and measuring on Ammonia-N concentrations in various surface water can be realized; average substrate adding standard recovery rates of lake water, river water and groundwater are respectively 100.4 percent, 95.2 percent and 101.7 percent; besides, the analysis speed is high, and a result is not remarkably different from that of a indophenol blue spectrophotometry method.

The invention provides a determination method for o-phthalaldehyde. The determination method comprises the following steps: reacting an excess hydroxylamine hydrochloride solution and a triethanolamine solution with o-phthalaldehyde; and titrating a reacted solution by 0.1mol/L sulfuric acid standard solution while heating. Therefore, a titrating solution is sufficiently titrated and reacted, and the accuracy of determination on the content of o-phthalaldehyde in disinfection liquid is greatly improved. The determination method has the advantages of simplicity in operation and high precision.

The invention relates to an impurity analysis method for multiple vitamin preparations. The method comprises the following steps of preparing a test solution of the vitamin preparations; dissolving o-phthalaldehyde and 2-mercaptoethanol based on a mass ratio of 1 to (2-5) in methanol, adding a boric acid buffer solution, and adjusting the pH value to 3-5 to obtain a derivatization reagent; and taking the test solution, adding the derivatization reagent to carry out online derivatization reaction, and injecting the derivatized test solution into a high performance liquid chromatograph, therebydetecting impurities in the test solution. According to the method, folic acid impurity A, folic acid impurity D, p-aminobenzoic acid and 3-aminopropanol in the vitamin preparations can be effectivelydetected; and the method is high in sensitivity and good in specificity.

The invention relates to a content detection method for lysine of a compound ketoacid tablet, which comprises the following steps: preparing an o-phthalaldehyde reaction liquid (OPA reagent) with the concentration of 10 mg/ml, wherein 2-3 percent (v/v) of 3-thiohydracrylic acid is contained; afterwards, mixing lysine and the reaction liquid at ratio of 1:1(v/v) in a borate buffer system with a pH valve of 10.2 plus or minus 0.1 through an automatic pre-column derivation method, and then carrying out ambient temperature reaction; and finally, adopting a high performance liquid chromatography to measure the content of the lysine at 338 nm. The method provided by the invention can effectively solve the problem that bimodality of the lysine occurs in the current OPA pre-column derivation method, improves the sample introduction precision of the lysine in the detection through the automatic pre-column derivation method, greatly shortens the detection time, and is particularly suitable for detecting the content of the lysine of amino acid samples in a laboratory.

The invention belongs to the technical field of disinfectant detection, and relates to test paper for detecting phthalic dicarboxaldehyde and preparation and detection methods of the test paper. According to the test paper, a color indicator is fixed on plain paper; the color indicator is selected from one or combinations of multiple of an amino-acid ester compound, arylamine and/or alkyl primary amine. According to the test paper for detecting the phthalic dicarboxaldehyde and the preparation and detection methods of the test paper, when the prepared test paper detects the concentration of the phthalic dicarboxaldehyde in disinfectant, dissolving of the color indicator fixed on the test paper in the disinfectant is reduced, so that the test paper and disinfectant contact time and the developing time have little influence on a phthalic dicarboxaldehyde concentration detection result, and the detection result is more real and reliable.

The invention relates to a method for synthesizing planar chiral diferrocene phosphine diimide, and this ligand contains diimine and has a planar chiral diferrocene structure. This compound is condensed of planar chiral ferroceneprimary amide and various dialdehyde compounds under the existence of shrinking agent. The dialdehyde compounds can be p-phthalaldehyde, o-phthalaldehyde, m-phthalaldehyde, phenyl dialdehyde, butyl dialdehyde, glutaric dialdehyde, etc. and the method has the advantages of simple synthesizing route, simple operation, easy purification, and high yield. The invention is applied to the catalytic reactions such as aromatic ketone unsymmetrical hydrogen transfer reaction and can achieve very high yield and enantiotropic selectivity.

The invention discloses an environment disinfectant for livestock cultivation, which comprising the following components according to part by weight: 5 to 10 parts of potassium permanganate, 1 to 3 parts of dichloroacetic acid, 1 to 4 parts of phthalic dicarboxaldehyde and 90 parts of water. The environment disinfectant for livestock cultivation has the advantages of simple preparation technology, low cost and obvious effect.

The invention provides a water quality monitoring instrument and a water quality monitoring method. The water quality monitoring instrument comprises a digestion system, a circulating system, a detection system, a control system and an upper computer system, wherein the digestion system is used for carrying out catalytic decomposition on water samples, and promoting reagent solutions to react with the water samples; the circulating system is used for providing reagent solutions and water samples required by spectral analysis, enabling orthophosphate to sequentially react with antimony potassium molybdate and ascorbic acids in the reagent solutions so as to obtain phosphomolybdenum blue solution, and enabling o-phthalaldehyde in the reagent solutions to react with ammonia nitrogen in the water samples so as to obtain an isoindole derivative solution; the detection system is used for respectively detecting the absorbance value of the phosphomolybdenum blue solution and the fluorescence value of the isoindole derivative solution; the control system is used for controlling the adding sequence of the reagent solutions in the circulating system, controlling the starting and stopping of the digestion system and the control system, and generating a corresponding detecting result according to the absorbance value and the fluorescence value; and the upper computer system is used for providing the input and display of man-machine interaction for operators. Through optimization design on an internal structure, the contents of ammonia nitrogen and total phosphorus in a water sample are simultaneously measured, thereby reducing the cost of water quality detection, and improving the efficiency of water quality monitoring.

The invention discloses a green preparation technology of phthalic dicarboxaldehyde, which takes 1, 3 -phthalan as raw material, hydrogen peroxide as an oxidant and immobilized rare earth metal and polyoxometallate (heteropoly acid) as catalysts, and water as solvent, and under the invigorating effect of ultrasonic field, the phthalic dicarboxaldehyde product is obtained through the processing steps of catalytic oxidation, ligarine recrystallization, suction filtration and the like. Compared with the prior art, the green preparation technology provided by the invention has the advantages as followings: the process reaction conditions are mild, the preparation process is simple, raw materials are easy to access, the reaction time is short, the product purity is high, zero contamination, small energy consumption and green economy are achieved, and the green preparation technology facilitates the popularization and the use of phthalic dicarboxaldehyde in the industries such as chemical engineering, medical and the like.