9 results about "O-Phthalaldehyde" patented technology

A method for measuring the concentration of phthalic dicarboxaldehyde (OPA) in a sample is provided. The method includes measuring the absorbance of the OPA at a specific wavelength using a spectrophotometer, and converting the absorbance value as a function of temperature (slope) into a concentration value using a standard curve. The methods are specific (selective), simple, rapid, and highly accurate, making them suitable for use in various applications, such as monitoring OPA concentrations in disinfection / sterilization industrial environments.

The application discloses a synthesis method of o-phthalaldehyde. O-xylene, a halogenated reagent and an organic solvent are mixed, and under ultraviolet light irradiation, a bromination product with different bromination degrees is generated by reaction; water is added into the reaction liquid, and the reaction is continuously carried out; after the reaction is completed, the organic solvent and water are removed by evaporation, and a crude ɑ, ɑ, ɑ', ɑ'-tetra-brominated o-xylene product is obtained; the crude product is recrystallized to obtain ɑ, ɑ, ɑ', ɑ'-tetra-brominated o-xylene product; and the ɑ, ɑ, ɑ', ɑ'-tetra-brominated o-xylene is hydrolyzed to obtain o-phthalaldehyde. The synthesis method optimizes the bromination and hydrolysis process, and by adding appropriate water in the o-xylene bromination process, the yield of the final product o-phthalaldehyde is improved.

The application discloses a kind of meat freshness rapid test paper, belong to food rapid detection technical field.The test paper is with water absorption filter paper as base material, and is loaded by the reaction color developing system consisting of o-Phthaldehyde, mercapto glycolic acid, sodium acetate buffer and fast blue B salt, can occur specific color reaction with the biological amine (putrescine) generated in the meat spoilage process, and realizes freshness rapid judgment by color ladder change.The detection time of the application is only 3-5 minutes, without large instrument, simple operation, high sensitivity, low cost, wide spectrum, can be used for on-site rapid screening of pork, beef, mutton, frozen shrimp, frozen chicken breast and other various meats, and is widely applicable to raw material acceptance of meat processing enterprises, market supervision law enforcement and cold chain logistics quality monitoring.

The invention belongs to the field of protein hydrogel materials, and particularly relates to elastin-like and a preparation method and application of elastin-like hydrogel. According to the present invention, the two elastin-like proteins A and B having different amino acid sequences are utilized to form the cross-linked network through the isopeptide bond, such that the mechanical strength of the polymer hydrogel composed of the four-arm polyethylene glycol amine, 4-arm PEG-NH2 and the o-phthalaldehyde modified four-arm polyethylene glycol, 4-arm PEG-OPA can be effectively improved, the adhesion can be improved, and the cross-linked network can be used for the preparation of the polymer hydrogel, such that the cross-linked network can be formed through the cross-linked network, such that the cross-linked network can be used for the preparation of the polymer hydrogel, and the use amount of the polymer hydrogel can be reduced. The obtained elastin-like hydrogel has the advantages of strong wet tissue adhesion capacity, good biocompatibility, injectable use and the like, and is beneficial to application in the biomedical fields of hemostasis, tissue repair and the like.

This invention discloses an amino acid assay kit based on the o-phthalaldehyde (OPA) method, its preparation method, and its application. Belonging to the field of amino acid detection and analysis technology, it is used for rapid and accurate quantitative detection of amino acids in samples. The amino acid assay kit can not only measure the total amino acid content but also analyze specific amino acids and dipeptides such as alanine glutamine, meeting diverse analytical needs. This amino acid assay kit is suitable for batch testing and supports high-throughput laboratory applications. By optimizing the reagent formulation and operating procedures, this invention effectively simplifies the detection steps, improves detection sensitivity and specificity, and fills the gap in the market for OPA-based assay kits. This invention can be widely applied in nutrition, medicine, clinical diagnostics, and drug development, providing a convenient and efficient amino acid analysis tool for life science and medical research.

The present application relates to a kind of diacetylene functionalized covalent organic framework material and its preparation method and application, to 4,4',4'',4'''-(pyrene-1,3,6,8-tetrayl) tetraphenylamine, 5,5'- (but-1,3-diethynyl-1,4-diyl) diisophthalaldehyde as building unit, synthesis using Schiff base reaction.The diacetylene functionalized covalent organic framework material of the present application has good chemical stability, and can be used as carrier by metal coordination effect Controllable growth silver nanoparticles to obtain composite material, the composite material in sodium borohydride catalytic 4-nitrophenol reduction reaction has shown excellent catalytic activity and cycle stability, thus has important application potential in the field such as environmental remediation and green chemistry.

The invention discloses a pathogenic nucleic acid extraction method based on reverse nucleic acid grabbing, which comprises the following steps: adding a sephadex microsphere solution with the surface modified with functional molecules and a lysis buffer into a bacterial liquid containing pathogenic bacteria, oscillating, inserting filter paper with negative electricity, incubating, taking out the filter paper, centrifuging, and taking out supernatant to obtain purified pathogenic nucleic acid, the functional molecules are o-phthalaldehyde, phenylboronic acid and hydrophobic molecules, the lysate contains lauryl sodium sulfate, disodium hydrogen phosphate and ethylenediamine tetraacetic acid, and the pH value of the lysate is 10.0-10.5. The method has the advantages of simplicity and convenience in operation, high extraction speed, high nucleic acid quality, high nucleic acid extraction rate and the like, and can provide a high-quality nucleic acid sample for molecular diagnosis.

Disclosed are methods for constructing conjugates. An amine-containing component and a thiol-containing component, in comparable mole ratios, react with an ortho-phthalaldehyde in one step, to generate a conjugate bearing a substituted or an unsubstituted 1-thio-isoindole structure in the presence of an additive. The method provides a platform for the efficient and practical constructions of bioactive modular motifs.

This invention discloses a method for ammonia nitrogen detection. The apparatus used in the detection method includes a flow mechanism and a detection mechanism. One end of the flow mechanism receives an external water sample to be tested, and the other end is connected to the detection mechanism. The two ends of the detection mechanism serve as an inlet and an outlet, respectively, both connected to a liquid flow tube provided in the flow mechanism. The flow mechanism guides the water sample to be tested through the liquid flow tube for cyclic mixing before flowing into the detection mechanism for ammonia nitrogen concentration detection. The ammonia nitrogen detection method proposed in this invention is a flow analysis turbidity correction method for the detection of ammonia nitrogen by the orthophthalaldehyde fluorescence method, and establishes a relationship model between turbidity and ammonia nitrogen concentration. This method can significantly reduce the interference of turbidity on the fluorescence method for ammonia nitrogen detection. The method has a wide applicable turbidity correction range and is suitable for ammonia nitrogen detection correction in complex water samples.