High-level disinfectant and preparation method thereof

Abstract

The invention belongs to the technical field of disinfectants and discloses a high-level disinfectant and a preparation method thereof.The high-level disinfectant is prepared from, by mass, 0.1%-3% of phthalic dicarboxaldehyde, 0.2%-0.8% of chelating agent, 2%-5% of penetrating agent, 0.3%-2% of modified starch, 1%-2% of acrylic polymer, 0.1%-0.3% of single-chain quaternary ammonium salt, 0.1%-0.3% of double-chain quaternary ammonium salt, 0.4%-5% of corrosion inhibitor and the balance purified water. Modified starch and polyacrylic acid are fully dissolved in a high-temperature stage, molecular chains are fully expanded, more active sites are exposed, and then the temperature is gradually reduced, so that the molecular chains are orderly arranged, a compact and uniform network structure is formed, and effective release of bactericidal components is ensured. Meanwhile, the single-chain quaternary ammonium salt and the double-chain quaternary ammonium salt are complementary through membrane destruction and internal destruction respectively and have a synergistic effect with the o-phthalaldehyde, so that the sterilization effect is remarkably improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of disinfectants, in particular to a high-level disinfectant and a preparation method thereof. BACKGROUND

[0002] With the improvement of medical technology, fine instruments dominated by digestive endoscopes are used for clinical diagnosis and treatment. With the improvement of medical level, the cleaning and disinfection of medical equipment and instruments are also increasingly demanding. At present, the commonly used disinfection and sterilization methods of medical instruments include pressure steam sterilization, dry heat sterilization, low-temperature gas sterilization, chemical disinfectant immersion disinfection and sterilization, etc. New digestive endoscopes, ultra-fine gastroscopes, etc. are precise diagnostic and treatment instruments that are repeatedly used in the human body. Due to their special material and complex structure, they are difficult to clean and disinfect. If these instruments are not thoroughly cleaned and disinfected, it will increase the risk of hospital infection.

[0003] The commonly used disinfectant at present is glutaraldehyde, but glutaraldehyde needs to be activated before use, the operation is complicated and the concentration used is high. In recent years, domestic and foreign scholars have proposed using o-phthaldehyde disinfectant to replace glutaraldehyde for endoscope disinfection. O-phthaldehyde is a highly efficient aldehyde disinfectant with low corrosion, small irritation, high efficiency, etc. It is suitable for the disinfection of various medical instruments and has been widely used in the disinfection of digestive endoscopes. As a single disinfectant, o-phthaldehyde has a slow killing speed on bacterial spores, which is difficult to meet the high-efficiency disinfection of endoscopes. In addition, the use of o-phthaldehyde can cause "gray staining". The surface of the object with gray staining is difficult to clean, which requires a lot of time and effort to clean. The coloring on the skin usually needs to be removed after several days, which brings additional problems to the cleaning of the medical environment and medical instruments. Quaternary ammonium salt disinfectants cannot achieve high-level disinfection standards (such as insufficient killing rate on Mycobacterium tuberculosis and spores) when used alone.

[0004] Patent CN103125510A discloses a compound o-phthaldehyde polyquaternary ammonium salt cationic surfactant disinfectant and its preparation and application. The operation method is to dissolve o-phthaldehyde in alcohol, add purified water, polyquaternary ammonium salt cationic surfactant, and ethylenediaminetetraacetic acid, and stir and dissolve. The disinfectant prepared by this method does not solve the problem of o-phthaldehyde gray staining.

[0005] Patent CN117837588A discloses a medical device o-phthaldehyde disinfectant and a preparation method thereof. The medical device o-phthaldehyde disinfectant includes the following components in mass fraction: o-phthaldehyde 1-3 parts, chelating agent 0.5-1 part, salicylic acid 0.2-0.5 part, buffer 0.2-0.5 part, and modified polyacrylic acid 1-3 parts. This method only modifies o-phthaldehyde and does not mention using other disinfectants to improve the function of the disinfectant.

[0006] Patent CN101161070A a double chain quaternary ammonium salt complex o-phthalaldehyde disinfectant, its main technical points are double chain quaternary ammonium salt complex o-phthalaldehyde disinfectant preparation raw material is according to molar content ratio is: double chain quaternary ammonium salt 2M, o-phthalaldehyde 1M, the prepared disinfectant is non-toxic, almost no odor, with the bacteriostatic disinfection efficacy of double chain quaternary ammonium salt and o-phthalaldehyde, the method prepared composite o-phthalaldehyde, o-phthalaldehyde ash problem is not solved.

[0007] Patent CN105379712A discloses a kind of o-phthalaldehyde disinfectant for endoscope cleaning and its preparation process, which is composed of o-phthalaldehyde, cosolvent, chelating agent, regulator, organic acid, penetrant, corrosion inhibitor, foam inhibitor, antioxidant and water. This method avoids the ash problem, but does not mention using composite disinfectant to improve the ability to kill bacterial spores with o-phthalaldehyde.

[0008] The o-phthalaldehyde disinfectant prepared by the above-mentioned existing technologies cannot solve the ash problem of o-phthalaldehyde, and the ability of the prepared disinfectant is relatively single. SUMMARY

[0009] To solve the above-mentioned problems existing in the prior art, the purpose of the present application is to provide a high-level disinfectant and its preparation method, which can improve the ability to kill bacterial spores of the disinfectant while reducing the ashing condition.

[0010] To solve the above-mentioned problems, the present application provides the following technical solutions: In a first aspect, the present application provides a high-level disinfectant, which comprises the following components by mass fraction: o-phthalaldehyde 0.1-3%, chelating agent 0.2-0.8%, penetrant 2-5%, modified starch 0.3-2%, acrylic acid polymer 1-2%, single-chain quaternary ammonium salt 0.1-0.3%, double-chain quaternary ammonium salt 0.1-0.3%, corrosion inhibitor 0.4-5%, and the balance is purified water.

[0011] In an embodiment of the present application, a high-level disinfectant comprises the following components by mass fraction: o-phthalaldehyde 1.5-2%, chelating agent 0.7-0.8%, penetrant 4-5%, modified starch 1.8-2%, acrylic acid polymer 1.8-2%, single-chain quaternary ammonium salt 0.25-0.3%, double-chain quaternary ammonium salt 0.25-0.3%, corrosion inhibitor 3-4%, and the balance is purified water.

[0012] In an embodiment of the present application, the chelating agent is selected from at least one of sodium ethylenediaminetetraacetate, citrate and pyrophosphate.

[0013] In an embodiment of the present application, the penetrant is selected from at least one of an alcohol, an ether.

[0014] In an embodiment of the present application, the alcohol is selected from at least one of ethanol, isopropanol, n-butanol, isobutanol, ethylene glycol, propylene glycol, 1,3-propanediol, diethylene glycol, PEG 200, PEG 400, glycerol, trimethylolpropane.

[0015] In an embodiment of the present application, the ether is selected from at least one of a fatty ether, a glycol ether, a polyether.

[0016] In an embodiment of the present application, the fatty ether is selected from at least one of diethyl ether, isopropyl ether, methyl tert-butyl ether.

[0017] In an embodiment of the present application, the glycol ether is selected from at least one of ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monobutyl ether.

[0018] In an embodiment of the present application, the polyether is selected from at least one of polyethylene glycol monomethyl ether, polyethylene glycol dimethyl ether.

[0019] In an embodiment of the present application, the corrosion inhibitor is selected from at least one of sodium salt of ethylenediaminetetraacetic acid, citrate, pyrophosphate.

[0020] In an embodiment of the present application, the modified starch is selected from at least one of cationic starch, esterified starch.

[0021] In an embodiment of the present application, the cationic starch is selected from at least one of quaternary ammonium group cationic starch or tertiary ammonium group cationic starch.

[0022] In an embodiment of the present application, the acrylic polymer is selected from at least one of polyacrylic acid, methyl acrylate copolymer, polymethyl methacrylate.

[0023] In an embodiment of the present application, the average molecular weight of the acrylic polymer is 100000-150000.

[0024] In an embodiment of the present application, the single-chain quaternary ammonium salt is selected from at least one of benzalkonium chloride, benzalkonium bromide, octadecyl trimethyl ammonium chloride.

[0025] In an embodiment of the present application, the double-chain quaternary ammonium salt is selected from at least one of didecyl dimethyl ammonium chloride, didodecyl dimethyl ammonium bromide.

[0026] In a second aspect, the present application provides a preparation method of a high-level disinfectant, comprising the following steps: The modified starch, acrylic acid polymer and o-phthalaldehyde are sequentially added into purified water in a mass fraction ratio, mixed and stirred uniformly at a rotating speed of 200-500 r / min to obtain a first mixed solution; The first mixed solution is mixed with a penetrant, a corrosion inhibitor and a chelating agent, stirred for 30-50 min at a rotating speed of 200-500 r / min to obtain a second mixed solution; The second mixed solution is mixed with a single-chain quaternary ammonium salt and a double-chain quaternary ammonium salt, stirred for 15-50 min at a rotating speed of 200-500 r / min to obtain a high-level disinfectant.

[0027] In an embodiment of the present application, the purified water is heated to 55-60 ℃, the modified starch is added and stirred and dissolved for 45-50 min at a rotating speed of 200-500 r / min, the acrylic acid polymer is added and continuously stirred for 45-50 min at a rotating speed of 200-500 r / min, the temperature is lowered to 45-50 ℃, the o-phthalaldehyde is added and stirred and dissolved for 35-50 min at a rotating speed of 200-5000 r / min to obtain the first mixed solution.

[0028] In an embodiment of the present application, the first mixed solution is lowered to 5-10 ℃ at a rate of 1.5-2 ℃ per minute, and the penetrant, the corrosion inhibitor and the chelating agent are mixed.

[0029] Compared with the prior art, the present application has the following beneficial effects: (1) The present application provides a high-level disinfectant and a preparation method thereof. The modified starch and the polyacrylic acid are fully dissolved at a high temperature stage, the molecular chains are fully unfolded, and more active sites are exposed. Then the molecular chains are orderly arranged by gradually lowering the temperature, and a dense and uniform network structure is formed, thereby ensuring the effective release of the sterilization components.

[0030] (2) The present application provides a high-level disinfectant and a preparation method thereof. The single-chain quaternary ammonium salt and the double-chain quaternary ammonium salt form complementation through membrane damage and internal damage respectively, and form a synergistic effect with o-phthalaldehyde, thereby significantly improving the sterilization effect.

[0031] (3) The present application provides a high-level disinfectant and a preparation method thereof. The modified starch and the polyacrylic acid form a network structure through complementation, thereby effectively controlling the release and distribution of the disinfectant, reducing the adhesion of residues, and significantly reducing the gray staining. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0033] The materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained commercially. Example 1

[0034] The high-level disinfectant provided in this example 1 has the following formulation: o-Phthalaldehyde 2%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.3%, double-chain quaternary ammonium salt (didecyldimethylammonium chloride) 0.3%, polyacrylic acid (average molecular weight 100000-150000) 2%, modified starch (3-chloro-2-hydroxypropyltrimethylammonium chloride modified starch with a degree of substitution of 0.04) 2%, penetrating agent (a mixture of ethanol and propylene glycol with a mass ratio of 2:1) 5%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate with a mass ratio of 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance being purified water.

[0035] The preparation method is as follows: The purified water is heated to 60°C, the modified starch is added, and stirred and dissolved for 50 min at a speed of 500 r / min to fully swell the modified starch and fully extend the molecular chains.

[0036] The polyacrylic acid is added, and stirring and dissolution are continued for 50 min at a speed of 500 r / min to fully dissolve the polyacrylic acid and form preliminary interactions with the extended molecular chains of the modified starch.

[0037] The mixture is cooled to 45°C, the o-phthalaldehyde is added, and stirring and dissolution are carried out for 40 min at a speed of 500 r / min to obtain a first mixture.

[0038] The first mixture is cooled to 5°C at a rate of 2°C per minute, the penetrating agent, corrosion inhibitor, and chelating agent are added, and stirring and dissolution are carried out for 50 min at a speed of 500 r / min to obtain a second mixture.

[0039] The second mixture is mixed with the single-chain quaternary ammonium salt and double-chain quaternary ammonium salt, stirring is carried out for 40 min at a speed of 500 r / min to obtain the high-level disinfectant. Example 2

[0040] The high-level disinfectant provided in this example 2 has the following formulation: o-Phthalaldehyde 1.5%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.25%, double-chain quaternary ammonium salt (didecyldimethylammonium chloride) 0.25%, polyacrylic acid (average molecular weight 100000-150000) 1.8%, modified starch (3-chloro-2-hydroxypropyltrimethylammonium chloride modified starch with a degree of substitution of 0.04) 1.8%, penetrating agent (a mixture of ethanol and propylene glycol with a mass ratio of 2:1) 4%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate with a mass ratio of 1:1) 3%, chelating agent (sodium citrate) 0.7%, and the balance being purified water.

[0041] The preparation method is as follows: Purified water is heated to 55°C, modified starch is added, and stirring and dissolving are performed for 45 min at a rotation speed of 450 r / min.

[0042] Polyacrylic acid is added, and stirring and dissolving are continuously performed for 50 min at a rotation speed of 500 r / min.

[0043] The mixed solution is cooled to 45°C, o-phthaldehyde is added, and stirring and dissolving are performed for 40 min at a rotation speed of 500 r / min to obtain a first mixed solution.

[0044] The first mixed solution is cooled to 10°C at a rate of 1.5°C per minute, a penetrant, a corrosion inhibitor and a chelating agent are added, and stirring and dissolving are performed for 50 min at a rotation speed of 500 r / min to obtain a second mixed solution.

[0045] The second mixed solution is mixed with a single-chain quaternary ammonium salt and a double-chain quaternary ammonium salt, stirring is performed for 40 min at a rotation speed of 500 r / min to obtain a high-level disinfectant. Example 3

[0046] The high-level disinfectant provided in this example 3 has the following formula: o-phthaldehyde 2%, a single-chain quaternary ammonium salt (a complex of benzalkonium chloride and benzyl bromide, mass ratio 1:1) 0.3%, a double-chain quaternary ammonium salt (a complex of double-cucalyptus dimethyl ammonium chloride and double-dodecyl dimethyl ammonium bromide, mass ratio 1:1) 0.3%, a complex of polyacrylic acid and polyacrylic acid methyl ester (mass ratio 1:1, average molecular weight 100000-150000) 2%, modified starch (3-chloro-2-hydroxypropyl trimethyl ammonium chloride modified starch with a degree of substitution of 0.04) 2%, a penetrant (a mixture of ethanol and propylene glycol, mass ratio 2:1) 5%, a corrosion inhibitor (a complex of sodium citrate and sodium pyrophosphate, mass ratio 1:1) 4%, a chelating agent (ethylenediamine tetraacetic acid disodium) 0.8%, and the balance is purified water.

[0047] The preparation method is as follows: Purified water is heated to 60°C, modified starch is added, and stirring and dissolving are performed for 50 min at a rotation speed of 500 r / min.

[0048] Polyacrylic acid is added, and stirring and dissolving are continuously performed for 50 min at a rotation speed of 500 r / min.

[0049] The mixed solution is cooled to 45°C, o-phthaldehyde is added, and stirring and dissolving are performed for 40 min at a rotation speed of 500 r / min to obtain a first mixed solution.

[0050] The first mixed solution is cooled to 5°C at a rate of 2°C per minute, a penetrant, a corrosion inhibitor and a chelating agent are added, and stirring and dissolving are performed for 50 min at a rotation speed of 500 r / min to obtain a second mixed solution.

[0051] The second mixture is mixed with the single-chain quaternary ammonium salt and the double-chain quaternary ammonium salt, stirred for 40 min at a speed of 500 r / min, to obtain the high-level disinfectant. Example 4

[0052] The high-level disinfectant provided by the present example 4 has the following formula: o-Phthalaldehyde 2.5%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.4%, double-chain quaternary ammonium salt (dipalmityl dimethyl ammonium chloride) 0.1%, polyacrylic acid (average molecular weight 100000-150000) 1%, modified starch (3-chloro-2-hydroxypropyl trimethyl ammonium chloride modified starch with a degree of substitution of 0.04) 2.5%, penetrating agent (a mixture of ethanol and propylene glycol with a mass ratio of 2:1) 5%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate with a mass ratio of 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance being purified water.

[0053] The preparation method is as follows: The purified water is heated to 60℃, and the modified starch is added and stirred and dissolved for 50 min at a speed of 500 r / min.

[0054] The polyacrylic acid is added, and stirring and dissolution are continued for 50 min at a speed of 500 r / min.

[0055] The mixture is cooled to 45℃, and the o-phthalaldehyde is added and stirred and dissolved for 40 min at a speed of 500 r / min, to obtain the first mixture.

[0056] The first mixture is cooled to 5℃ at a rate of 2℃ per minute, and the penetrating agent, the corrosion inhibitor, and the chelating agent are added and stirred and dissolved for 50 min at a speed of 500 r / min, to obtain the second mixture.

[0057] The second mixture is mixed with the single-chain quaternary ammonium salt and the double-chain quaternary ammonium salt, stirred for 40 min at a speed of 500 r / min, to obtain the high-level disinfectant.

[0058] Comparative Example 1 The disinfectant provided by the present comparative example 1 has the following formula: o-Phthalaldehyde 2%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.3%, double-chain quaternary ammonium salt (dipalmityl dimethyl ammonium chloride) 0.3%, polyacrylic acid (average molecular weight 100000-150000) 2%, modified starch (3-chloro-2-hydroxypropyl trimethyl ammonium chloride modified starch with a degree of substitution of 0.04) 2%, penetrating agent (a mixture of ethanol and propylene glycol with a mass ratio of 2:1) 5%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate with a mass ratio of 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance being purified water.

[0059] The preparation method is as follows: Purified water was heated to 60℃, modified starch, polyacrylic acid, o-phthalaldehyde were added, and stirred and dissolved for 50 min at a speed of 500 r / min to obtain a first mixed solution.

[0060] The first mixed solution was directly added into penetrant, corrosion inhibitor, chelating agent, and stirred and dissolved for 50 min at a speed of 500 r / min to obtain a second mixed solution.

[0061] The second mixed solution was mixed with single-chain quaternary ammonium salt and double-chain quaternary ammonium salt, and stirred for 40 min at a speed of 500 r / min to obtain a disinfectant.

[0062] Comparative Example 2 Comparative Example 2 provides a disinfectant with a formula of: o-phthalaldehyde 2%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.3%, double-chain quaternary ammonium salt (bisquidimethylammonium chloride) 0.3%, polyacrylic acid (average molecular weight 100000-150000) 2%, modified starch (3-chloro-2-hydroxypropyl trimethyl ammonium chloride modified starch with a degree of substitution of 0.04) 2%, penetrant (a mixture of ethanol and propylene glycol with a mass ratio of 2:1) 5%, corrosion inhibitor (a complex of ethylenediaminetetraacetic acid disodium and sodium citrate with a mass ratio of 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance being purified water.

[0063] The preparation method is as follows: Purified water was heated to 60℃, and modified starch was added, and stirred and dissolved for 50 min at a speed of 500 r / min.

[0064] Polyacrylic acid was added, and stirring and dissolution was continued for 50 min at a speed of 500 r / min.

[0065] The mixed solution was cooled to 45℃, o-phthalaldehyde was added, and stirred and dissolved for 40 min at a speed of 500 r / min to obtain a first mixed solution.

[0066] The first mixed solution was naturally cooled to 25℃, penetrant, corrosion inhibitor, and chelating agent were added, and stirred and dissolved for 50 min at a speed of 500 r / min to obtain a second mixed solution.

[0067] The second mixed solution was mixed with single-chain quaternary ammonium salt and double-chain quaternary ammonium salt, and stirred for 40 min at a speed of 500 r / min to obtain a disinfectant.

[0068] Comparative Example 3 Comparative Example 3 provides a disinfectant with a formulation of o-Phthalaldehyde 2%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.3%, double-chain quaternary ammonium salt (didecyldimethylammonium chloride) 0.3%, polyacrylic acid (average molecular weight 100000-150000) 0.5%, modified starch (3-chloro-2-hydroxypropyltrimethylammonium chloride modified starch with a degree of substitution of 0.04) 0.5%, penetrating agent (a mixture of ethanol and propylene glycol with a mass ratio of 2:1) 5%, corrosion inhibitor (disodium ethylenediaminetetraacetate) 4%, chelating agent (sodium citrate) 0.8%, and the balance being purified water.

[0069] The preparation method is as follows: The purified water is heated to 60°C, the modified starch, polyacrylic acid, and o-Phthalaldehyde are added, and stirred and dissolved for 50 min at a speed of 500 r / min to obtain a first mixed solution.

[0070] The first mixed solution is directly added to the penetrating agent, corrosion inhibitor, and chelating agent, and stirred and dissolved for 50 min at a speed of 500 r / min to obtain a second mixed solution.

[0071] The second mixed solution is mixed with the single-chain quaternary ammonium salt and double-chain quaternary ammonium salt, and stirred for 40 min at a speed of 500 r / min to obtain the disinfectant.

[0072] Comparative Example 4 Comparative Example 4 provides a disinfectant with a formulation of o-Phthalaldehyde 2%, double-chain quaternary ammonium salt (didecyldimethylammonium chloride) 0.3%, polyacrylic acid (average molecular weight 100000-150000) 2%, modified starch (3-chloro-2-hydroxypropyltrimethylammonium chloride modified starch with a degree of substitution of 0.04) 2%, penetrating agent (a mixture of ethanol and propylene glycol with a mass ratio of 2:1) 5%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate with a mass ratio of 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance being purified water.

[0073] The preparation method is as follows: The purified water is heated to 60°C, the modified starch is added, and stirred and dissolved for 50 min at a speed of 500 r / min.

[0074] The polyacrylic acid is added, and stirring and dissolution are continued for 50 min at a speed of 500 r / min.

[0075] The mixed solution is cooled to 45°C, o-Phthalaldehyde is added, and stirred and dissolved for 40 min at a speed of 500 r / min to obtain a first mixed solution.

[0076] The first mixed solution is cooled to 5°C at a rate of 2°C per minute, the penetrating agent, corrosion inhibitor, and chelating agent are added, and stirred and dissolved for 50 min at a speed of 500 r / min to obtain a second mixed solution.

[0077] The second mixture was mixed with the single-chain quaternary ammonium salt, stirred for 40 min at a speed of 500 r / min, to obtain the disinfectant.

[0078] Comparative Example 5 Comparative Example 5 provides a disinfectant with the following formula: o-Phthalaldehyde 2%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.3%, polyacrylic acid (average molecular weight 100000-150000) 2%, modified starch (3-chloro-2-hydroxypropyl trimethyl ammonium chloride modified starch with a degree of substitution of 0.04) 2%, penetrating agent (a mixture of ethanol and propylene glycol with a mass ratio of 2:1) 5%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate with a mass ratio of 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance being purified water.

[0079] The preparation method is as follows: The purified water was heated to 60°C, and the modified starch was added and stirred and dissolved for 50 min at a speed of 500 r / min.

[0080] The polyacrylic acid was added and stirring and dissolution was continued for 50 min at a speed of 500 r / min.

[0081] The mixture was cooled to 45°C, and the o-phthalaldehyde was added and stirred and dissolved for 40 min at a speed of 500 r / min, to obtain a first mixture.

[0082] The first mixture was cooled to 5°C at a rate of 2°C per minute, and the penetrating agent, corrosion inhibitor, and chelating agent were added and stirred and dissolved for 50 min at a speed of 500 r / min, to obtain a second mixture.

[0083] The second mixture was mixed with the single-chain quaternary ammonium salt, stirred for 40 min at a speed of 500 r / min, to obtain the disinfectant.

[0084] Comparative Example 6 Comparative Example 6 provides a disinfectant with the following formula: o-Phthalaldehyde 2%, polyacrylic acid (average molecular weight 100000-150000) 2%, modified starch (3-chloro-2-hydroxypropyl trimethyl ammonium chloride modified starch with a degree of substitution of 0.04) 2%, penetrating agent (a mixture of ethanol and propylene glycol with a mass ratio of 2:1) 5%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate with a mass ratio of 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance being purified water.

[0085] The preparation method is as follows: The purified water was heated to 60°C, and the modified starch was added and stirred and dissolved for 50 min at a speed of 500 r / min.

[0086] The polyacrylic acid was added and stirring and dissolution was continued for 50 min at a speed of 500 r / min.

[0087] Cool the mixed solution to 45℃, add o-phthaldehyde, stir and dissolve for 40 min at 500 r / min, to obtain a first mixed solution.

[0088] Cool the first mixed solution to 5℃ at a rate of 2℃ per minute, add the penetrant, corrosion inhibitor and chelating agent, stir and dissolve for 50 min at 500 r / min, to obtain the disinfectant.

[0089] Comparative Example 7 Comparative Example 7 provides a disinfectant with the following formula: o-phthaldehyde 2%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.3%, double-chain quaternary ammonium salt (bisquaternary ammonium chloride) 0.3%, polyacrylic acid (average molecular weight 100000-150000) 2%, penetrant (a mixture of ethanol and propylene glycol, mass ratio 2:1) 5%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate, mass ratio 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance being purified water.

[0090] The preparation method is as follows: Heat the purified water to 60℃, add the polyacrylic acid, stir and dissolve for 50 min at 500 r / min.

[0091] Cool the mixed solution to 45℃, add o-phthaldehyde, stir and dissolve for 40 min at 500 r / min, to obtain a first mixed solution.

[0092] Cool the first mixed solution to 5℃ at a rate of 2℃ per minute, add the penetrant, corrosion inhibitor and chelating agent, stir and dissolve for 50 min at 500 r / min, to obtain a second mixed solution.

[0093] Mix the second mixed solution with the single-chain quaternary ammonium salt and double-chain quaternary ammonium salt, stir for 40 min at 500 r / min, to obtain the disinfectant.

[0094] Comparative Example 8 Comparative Example 8 provides a disinfectant with the following formula: o-phthaldehyde 2%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.3%, double-chain quaternary ammonium salt (bisquaternary ammonium chloride) 0.3%, modified starch (3-chloro-2-hydroxypropyl trimethyl ammonium chloride modified starch with a degree of substitution of 0.04) 2%, penetrant (a mixture of ethanol and propylene glycol, mass ratio 2:1) 5%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate, mass ratio 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance being purified water.

[0095] The preparation method is as follows: Heat the purified water to 60℃, add the modified starch, stir and dissolve for 50 min at 500 r / min.

[0096] Cool to 45℃, add o-Phthaldehyde, stirring and dissolving 40 min, speed 500 r / min, to get the first mixed solution.

[0097] The first mixed solution is cooled to 5℃ at a rate of 2℃ per minute, and penetrant, corrosion inhibitor, chelating agent are added, stirring and dissolving 50 min, speed 500 r / min, to get the second mixed solution.

[0098] The second mixed solution is mixed with single-chain quaternary ammonium salt and double-chain quaternary ammonium salt, stirring 40 min, speed 500 r / min, to get the disinfectant.

[0099] Comparative Example 9 Comparative Example 9 provides a disinfectant with the following formula: o-Phthaldehyde 2%, single-chain quaternary ammonium salt (benzalkonium chloride) 0.3%, double-chain quaternary ammonium salt (bisquidimethylammonium chloride) 0.3%, penetrant (a mixture of ethanol and propylene glycol, mass ratio 2:1) 5%, corrosion inhibitor (a complex of disodium ethylenediaminetetraacetate and sodium citrate, mass ratio 1:1) 4%, chelating agent (sodium pyrophosphate) 0.8%, and the balance is purified water.

[0100] The preparation method is as follows: Purified water is heated to 45℃, o-Phthaldehyde is added, stirring and dissolving 40 min, speed 500 r / min, to get the first mixed solution.

[0101] The first mixed solution is cooled to 5℃ at a rate of 2℃ per minute, and penetrant, corrosion inhibitor, chelating agent are added, stirring and dissolving 50 min, speed 500 r / min, to get the second mixed solution.

[0102] The second mixed solution is mixed with single-chain quaternary ammonium salt and double-chain quaternary ammonium salt, stirring 40 min, speed 500 r / min, to get the disinfectant.

[0103] The disinfectants prepared in the above examples and comparative examples are tested for performance, and the test method is as follows, and the results are shown in Table 1.

[0104] The bacterial spore killing effect test is carried out according to the method specified in the "Disinfection Technical Specification" (2002 edition). Bacillus subtilis var. niger spores (ATCC 9372) are selected as the indicator bacteria, and a bacterial suspension is prepared, and the concentration is adjusted to 1×10 6 CFU / mL. The disinfectant is mixed with the bacterial suspension at a certain ratio, and after a specified time, the mixed solution is added to the neutralizer to terminate the disinfection effect. An appropriate amount of neutralized mixed solution is inoculated on nutrient agar plates and cultured at 37℃ for 48h, and the number of bacterial colonies is counted to calculate the killing rate.

[0105] The surface of the stainless steel sheet (5 cm x 5 cm) was evenly sprayed with the disinfectant, and after a specified period of action, it was rinsed with pure water and dried at room temperature. The color difference value (ΔE) of the surface of the stainless steel sheet was measured using a color difference meter. The smaller the ΔE value, the lighter the staining.

[0106] Metal corrosion tests were performed according to the method specified in the Disinfection Technical Standard (2002 edition). Three types of metal sheets (5 cm x 5 cm) were selected: stainless steel, copper, and aluminum, and the surfaces were polished to a uniform finish. The metal sheets were completely immersed in the disinfectant and soaked at 37°C for 72 h. The metal sheets were removed, rinsed with pure water, and dried at room temperature. The mass change of the metal sheets before and after soaking was measured using a precision electronic balance, and the corrosion rate (mg / dm²·d) was calculated. At the same time, the presence of obvious corrosion marks on the surface of the metal sheets was observed.

[0107] Table 1 ; As can be seen from Table 1, the high-level disinfectants prepared in Examples 1 to 4 all exhibit good bacterial spore killing effect and low staining effect, among which Example 1 has the best effect. First, the modified starch and polyacrylic acid are fully dissolved at the high temperature stage, the molecular chains are fully stretched, and more active sites are exposed. Then, the molecular chains are orderly arranged as the temperature gradually decreases, forming a dense and uniform network structure, thereby ensuring the effective release of the sterilization components.

[0108] In Comparative Example 1, the modified starch, polyacrylic acid, and o-phthaldehyde are added at once, which results in insufficient dissolution and interaction of the modified starch and polyacrylic acid, insufficient stretching of the molecular chains, insufficient exposure of active sites, and imperfect network structure, leading to uneven release of sterilization components, increased residues, and severe staining.

[0109] In Comparative Example 2, the temperature is naturally cooled to room temperature, and the sterilization effect is lower than that of Examples 1 to 4 but higher than that of Comparative Example 1.

[0110] In Comparative Example 3, the content of polyacrylic acid and modified starch is reduced, which cannot provide enough crosslinking points and network support, resulting in ineffective formation of the network structure. At the same time, the modified starch, polyacrylic acid, and o-phthaldehyde are added at once, which leads to poor sterilization effect and severe staining.

[0111] As can be seen from the results of Example 1 and Comparative Examples 4 to 6, the single-chain quaternary ammonium salt and the double-chain quaternary ammonium salt form complementarity through membrane disruption and internal disruption, respectively, and form a synergistic effect with o-phthaldehyde, significantly improving the sterilization effect.

[0112] As can be seen from the results of Example 1 and Comparative Examples 7 to 9, the network structure is formed by the complementarity of modified starch and polyacrylic acid, effectively controlling the release and distribution of the disinfectant, reducing the attachment of residues, and significantly reducing the staining.

[0113] The application has been described in detail with specific reference to particular embodiments and exemplifying examples, but it will be understood that these descriptions are not intended to limit the application to particular forms disclosed. Many modifications, equivalents and improvements created during the production of the application and its execution could be made by those skilled in the art without departing from the spirit and scope of the application. These modifications, equivalents and improvements are intended to fall within the scope of the application. The scope of the application is defined by the appended claims.

Claims

1. A high level disinfectant characterized in that, Comprise the following components by mass fraction: O-Phthaldehyde 0.1-3%, chelating agent 0.2-0.8%, penetrant 2-5%, modified starch 0.3-2%, acrylic acid polymer 1-2%, single chain quaternary ammonium salt 0.1-0.3%, double chain quaternary ammonium salt 0.1-0.3%, corrosion inhibitor 0.4-5%, the balance is purified water.

2. The high level disinfectant of claim 1, wherein, Comprise the following components by mass fraction: O-Phthaldehyde 1.5-2%, chelating agent 0.7-0.8%, penetrant 4-5%, modified starch 1.8-2%, acrylic acid polymer 1.8-2%, single chain quaternary ammonium salt 0.25-0.3%, double chain quaternary ammonium salt 0.25-0.3%, corrosion inhibitor 3-4%, the balance is purified water.

3. The high level disinfectant of claim 1, wherein, The chelating agent is selected from at least one of sodium salt of ethylenediaminetetraacetic acid, citrate, pyrophosphate; the penetrant is selected from at least one of alcohol, ether; the corrosion inhibitor is selected from at least one of sodium salt of ethylenediaminetetraacetic acid, citrate, pyrophosphate.

4. The high level disinfectant of claim 1, wherein, The modified starch is selected from at least one of cationic starch, esterified starch.

5. The high level disinfectant of claim 1, wherein, The acrylic acid polymer is selected from at least one of polyacrylic acid, methyl acrylate copolymer, polymethyl methacrylate, and the average molecular weight is 100000-150000.

6. The high level disinfectant of claim 1, wherein, The single chain quaternary ammonium salt is selected from at least one of benzalkonium chloride, benzalkonium bromide, octadecyl trimethyl ammonium chloride.

7. The high level disinfectant of claim 1, wherein, The double chain quaternary ammonium salt is selected from at least one of didecyldimethyl ammonium chloride, didodecyldimethyl ammonium bromide.

8. Process for the preparation of a high-level disinfectant according to any one of claims 1 to 7, characterized in that, Comprise the following steps: Modified starch, acrylic acid polymer, o-phthaldehyde are added into purified water in the order of mass fraction ratio, mixed and stirred, the rotating speed is 200-500r / min, to obtain a first mixed solution; The first mixed solution is mixed with penetrant, corrosion inhibitor, chelating agent, stirred for 30-50min, the rotating speed is 200-500r / min, to obtain a second mixed solution; The second mixed solution is mixed with single chain quaternary ammonium salt and double chain quaternary ammonium salt, stirred for 15-50min, the rotating speed is 200-500r / min, to obtain a high level disinfectant.

9. The preparation method of the high level disinfectant according to claim 8, wherein, Purified water is heated to 55-60℃, modified starch is added, stirred and dissolved for 45-50min, the rotating speed is 200-500r / min; Acrylic acid polymer is added, continue to stir for 45-50min, the rotating speed is 200-500r / min; Cool to 45-50℃, add o-phthaldehyde, stir and dissolve for 35-50min, the rotating speed is 200-5000r / min, to obtain a first mixed solution.

10. The method of claim 8, wherein the high level disinfectant is prepared by, The first mixed solution is cooled to 5-10℃ at a rate of 1.5-2℃ per minute, penetrant, corrosion inhibitor and chelating agent are added and mixed.