50 results about "Dinitrofluorobenzene" patented technology

The invention relates to a fluorescence enhancement type hydrogen sulfide fluorescent probe and particularly relates to a fluorescence enhancement type hydrogen sulfide fluorescent probe based on flavone derivatives. Flavone derivatives are directly reacted with 2,4-dinitrofluorobenzene, and a reaction product is subjected to column chromatography to generate a pure probe product. According to the fluorescence enhancement type hydrogen sulfide fluorescent probe, probe molecules have the maximum absorption wavelengths of 460nm, relatively good solubility in a 1.5mM CTAB water solution and stable optical performance; by adding the probe molecules together with hydrogen sulfide, the absorption wavelengths are blueshifted from 460nm to 450nm, and the intensity of a fluorescence spectrum is continuously enhanced at 612nm and is maximally enhanced by 96 times. According to the fluorescence enhancement type hydrogen sulfide fluorescent probe, the deficiencies that an existing fluorescent probe is slow in response to hydrogen sulfide and high in detection line, and detection wavelengths are in a visible light region are overcome; the probe molecules are high in sensitivity, stable in optical performance, relatively high in synthetic yield, strong in capability of identifying hydrogen sulfide, high in response speed, wide in detection range and low in detection lower limit, the response range is 0-50 mu M, and the detection limit is 0.09 mu M; therefore, the fluorescent probe has practical application values in the fields of biological chemistry, environmental science and the like.

The invention discloses a near-infrared fluorescent probe for high-selectivity detecting of thiophenol and a synthesis method and application thereof, and belongs to the technical field of chemical analysis and detection. The near-infrared fluorescent probe is obtained by reaction between a dicyanoisophorone-julolidine large pi system and 2,4-dinitrofluorobenzene, and has the following structure (please see the specifications for the structure). Fluorophore of the probe is the dicyanoisophorone-julolidine large pi system, and a response group to thiophenol is 2,4-dinitrophenoxy. Molecules of the probe have high selectivity and sensitivity to thiophenol, the detection range is 1-60 [mu]mol.L<-1>, and the limit of detection is 0.13 [mu]mol.L<-1>. The probe can be used for detecting thiophenol in water bodies, soil and cells.

The invention discloses an application of globeflower flavonoids in preparation of a medicine for treating itch dermatoses. The application range of globeflower flavonoids is expanded; a novel medicine is provided for treatment of itch dermatoses; from a pharmacology experiment, the globeflower flavonoids have good prevention and treatment effects on a mice chronic eczema model, have an obvious inhibiting effect on mice delayed-type allergic reaction caused by 2,4-dinitrofluorobenzene, have obvious anti-inflammatory effect in ear swelling in mice caused by xylene, and have a significant inhibiting effect on capillary permeability increase in mice caused by glacial acetic acid; and the globeflower flavonoids can have relatively good counteraction on guinea pig pruritus caused by histamine phosphate, are low in toxicity and safe to use, and have a relatively good application prospect.

The invention discloses a method for detecting amino acid content in calcium zinc gluconate oral solution. According to the detection method, pre-column derivatization-reversed-phase high-performanceliquid chromatography is adopted, and 2,4-dinitrofluorobenzene is taken as a derivatization reagent. The detection method comprises the following steps: (1) preparing a separation degree stock solution containing lysine hydrochloride and other impurity amino acids; (2) determining a corresponding relation between the dose of the derivatization reagent and the amino acid content in the separation degree stock solution; (3) preparing a test stock solution by utilizing calcium zinc gluconate oral solution, and carrying out derivation reaction, wherein the addition amount of the derivatization reagent is determined according to the corresponding relation determined in the step (2) and the amino acid content in the test stock solution; and (4) detecting by utilizing a reversed-phase high-performance liquid chromatograph. According to the method for detecting amino acid content in calcium zinc gluconate oral solution disclosed by the invention, the addition amount of the derivatization reagent is optimized, the pH value of a mobile phase is optimized, the detection process is excellent in repeatability, and the method is high in sensitivity and stable and reliable in appearance time.

The invention discloses a method for detecting amino acid content in calcium zinc gluconate oral solution. According to the detection method, pre-column derivatization-reversed-phase high-performanceliquid chromatography is adopted, and 2,4-dinitrofluorobenzene is taken as a derivatization reagent. The detection method comprises the following steps: (1) preparing a separation degree stock solution containing lysine hydrochloride and other impurity amino acids; (2) determining a corresponding relation between the dose of the derivatization reagent and the amino acid content in the separation degree stock solution; (3) preparing a test stock solution by utilizing calcium zinc gluconate oral solution, and carrying out derivation reaction, wherein the addition amount of the derivatization reagent is determined according to the corresponding relation determined in the step (2) and the amino acid content in the test stock solution; and (4) detecting by utilizing a reversed-phase high-performance liquid chromatograph. According to the method for detecting amino acid content in calcium zinc gluconate oral solution disclosed by the invention, the addition amount of the derivatization reagent is optimized, the pH value of a mobile phase is optimized, the detection process is excellent in repeatability, and the method is high in sensitivity and stable and reliable in appearance time.

The invention discloses an application of an imiquimod / dinitrofluorobenzene double drug-carrying nano carrier. The invention provides an application of the imiquimod / dinitrofluorobenzene double drug-carrying nano carrier in preparing a drug for treating cutaneous malignant melanoma. The administration route of the imiquimod / dinitrofluorobenzene double drug-carrying nano carrier is delivered by means of an ultrasonic conductometer in a targeted mode. The imiquimod / dinitrofluorobenzene double drug-carrying nano carrier disclosed by the invention improves the curative effect on malignant melanoma, improves the living quality of patients and prolongs the lifetime.

The pharmacological experiment shows that, the externally used preparation of Loratadine has very good allergy resisting and anti-inflammatory action to the rat passive cutaneous anaphylaxis (Rat PCA) model and dimethylbenzene caused mouse otitis model, in the experiment, it is found by chance that, the externally used preparation of Loratadine also has very fine inhibitory action to dinitrofluorobenzene caused mouse porphyria hypersensitivity (PTH), the novel pharmacological action mechanism of the Loratadine shows that the Loratadine external preparation has good therapeutic action to skin inflammations which mainly include porphyria hypersensitivity.

The invention relates to preparation and application of a hydrogen sulfide (H2S) near-infrared fluorescent probe, wherein the structural formula of the probe is shown in the specification. The invention provides the preparation method for synthesizing the fluorescent probe by taking isophorone-xanthene dye, 2,4-dinitrofluorobenzene and the like as raw materials. The fluorescent probe is a hydrogen sulfide fluorescent probe with large Stokes shift and near infrared emission. Firstly, the fluorescent probe shows good sensitivity to H2S, the linear range is 1 [mu]M-10 [mu]M, and the detection limit is 0.3 [mu]M; secondly, the fluorescent probe shows high selectivity on H2S, and is not influenced by various other ions, active oxygen and biological mercaptan; moreover, the fluorescent probe can quickly act with H2S, and the response time is within 10 minutes; and in addition, the fluorescent probe can also be applied to detection of H2S content in living cells.

The pharmacological experiment shows that, the externally used preparation of Loratadine has very good allergy resisting and anti-inflammatory action to the rat passive cutaneous anaphylaxis (Rat PCA) model and dimethylbenzene caused mouse otitis model, in the experiment, it is found by chance that, the externally used preparation of Loratadine also has very fine inhibitory action to dinitrofluorobenzene caused mouse porphyria hypersensitivity (PTH), the novel pharmacological action mechanism of the Loratadine shows that the Loratadine external preparation has good therapeutic action to skin inflammations which mainly include porphyria hypersensitivity.

The invention relates to the field of fine chemical industry, in particular to the design, synthesis and application of a two-photon ultra-low background fluorescent molecular probe based on enone rearrangement. The synthetic method of fluorescent probe adopts the following steps: (1) NDN preparation: 2-naphthol solution is added in sodium hydroxide solution, and the reagent of anhydrous and oxygen-free is processed; Add diazonium salt in ice bath, stir ; Acidify, dry, get NDN; (2) fluorescent probe DNHF‑H 2 Preparation of S: In an anhydrous and oxygen-free environment, weigh NDN and 2,4-dinitrofluorobenzene, add them to DMF and mix evenly; add potassium carbonate, stir; heat up to 40-60°C, react; cool to room temperature and add ice water, filtered with suction to obtain a fluorescent probe. Two-photon imaging of this probe reduces photodamage to living biological samples and fluorophores, reduces background absorption and scattering, and improves spatial resolution and sensitivity.