Glutathione fluorescence probe as well as preparation method and application thereof

A technology of fluorescent probe and glutathione, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of time-consuming and cumbersome preparation of invertase, and achieve easy large-scale production and detection sensitivity High, simple and easy preparation process

CN103289681AInactive Publication Date: 2013-09-11NANJING UNIV OF TECH

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Patent Information

Authority / Receiving Office: CN · China
Current Assignee / Owner: NANJING UNIV OF TECH
Publication Date: 2013-09-11
Estimated Expiration: Not applicable · inactive patent

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Abstract

The invention relates to a glutathione fluorescence probe as well as a preparation method and an application thereof. A probe structure is shown in a formula (I). The preparation method comprises the following steps of: dissolving 2,4-dinitrofluorobenzene, fluorescein and potassium carbonate into anhydrous dimethyl formamide (DMF); after reaction, preparing the glutathione fluorescence probe; the application of the glutathione fluorescence probe refers to detection on contents of glutathione having non-diagnostic property. According to the glutathione fluorescence probe as well as the preparation method and the application of the glutathione fluorescence probe, the fluorescence reaction only on the fluorescence probe and glutathione is carried out while the fluorescence reaction on the fluorescence probe and other amino acids is avoided, so that the glutathione fluorescence probe has high selectivity and specificity; the preparation process of the probe is easy and feasible, and the probe is easy for scale production.

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Description

technical field

[0001] The invention relates to a glutathione fluorescent probe, a preparation method and application thereof. Background technique

[0002] Thiols play an important role in the antioxidant system of cells and are important components of many proteins and small molecules in living organisms. Small molecule thiols mainly include glutathione (GSH), cysteine (Cys), and homocysteine (Hcy). A large number of physiological phenomena in organisms are considered to depend on the reactions involved in these thiol-containing thiols, such as redox reactions, methyl transfer reactions, and sulfur metabolism reactions in organisms. The concentration of thiols contained in living organisms is considered to be related to many diseases. Deficiency of thiols in the organism can lead to many health problems such as growth retardation, hair loss, lethargy, liver damage, weight loss, and some skin disorders. Glutathione is the most abundant small molecule thiol (1-10mM) i...

Claims

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