The invention relates to a high-performance liquid chromatography analytical method for N1,N1-diisopropyl ethylenediamine. The analytical method is characterized by including the following steps that 1, N1,N1-diisopropyl ethylenediamine standard solutions of different concentrations are prepared, excess 2,4-dinitrofluorobenzene is added into the standard solutions for a derivatization reaction, after the reaction is completed, high-performance liquid chromatography analysis is carried out on reaction solutions, and the DNB-N1,N1-diisopropyl ethylenediamine peak area corresponds to a N1,N1-diisopropyl ethylenediamine concentration drawing standard curve; 2, a test sample is prepared into a solution, excess 2,4-dinitrofluorobenzene is added into the solution to react, after the reaction is completed, high-performance liquid chromatography analysis is carried out on the reaction solution, and the content of N1,N1-diisopropyl ethylenediamine in the test sample is calculated. The analytical method is accurate, high in repeatability and sensitivity, and capable of being used for detecting N1,N1-diisopropyl ethylenediamine in the producing and placing processes of acotiamide hydrochloride and tablets thereof and detecting N1,N1-diisopropyl ethylenediamine in other samples.