A kind of two-photon ultra-low background fluorescent probe and its preparation method and application

A fluorescent probe and two-photon technology, applied in the field of analytical chemistry, can solve the problems of large background fluorescence interference, complex processing process, and large cell damage, and achieve the effects of improving detection sensitivity, reducing interference, and reducing sample damage.
CN110156630BActive Publication Date: 2022-02-08QUFU NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
QUFU NORMAL UNIV
Publication Date
2022-02-08

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Abstract

The invention relates to the field of fine chemical industry, in particular to the design, synthesis and application of a two-photon ultra-low background fluorescent molecular probe based on enone rearrangement. The synthetic method of fluorescent probe adopts the following steps: (1) NDN preparation: 2-naphthol solution is added in sodium hydroxide solution, and the reagent of anhydrous and oxygen-free is processed; Add diazonium salt in ice bath, stir ; Acidify, dry, get NDN; (2) fluorescent probe DNHF‑H 2 Preparation of S: In an anhydrous and oxygen-free environment, weigh NDN and 2,4-dinitrofluorobenzene, add them to DMF and mix evenly; add potassium carbonate, stir; heat up to 40-60°C, react; cool to room temperature and add ice water, filtered with suction to obtain a fluorescent probe. Two-photon imaging of this probe reduces photodamage to living biological samples and fluorophores, reduces background absorption and scattering, and improves spatial resolution and sensitivity.
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Description

technical field

[0001] The invention belongs to the field of analytical chemistry, and relates to a two-photon ultra-low background fluorescent probe and its preparation method and application. Background technique

[0002] At present, traditional techniques for detecting hydrogen sulfide include chromatographic analysis, colorimetry, and electrochemical analysis. These traditional detection techniques generally have the disadvantages of large sample consumption, complex processing process, great damage to cells, and cumbersome detection process. At present, the fluorescent probes that can be used to detect hydrogen sulfide have too much background fluorescence interference and low selectivity. However, this probe has the advantages of two-photon ultra-low background fluorescence and is easy to operate. Therefore, it is necessary to develop this probe. Contents of the invention

[0003] Fluorescent probes currently available for detecting hydrogen sulfide have too much ba...

Claims

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