The invention relates to a kit for quantitative detection of IGF-1 (
Insulin-like
Growth Factor-1) and a preparation and detection method thereof. The kit comprises a calibrator, a
quality control product, an
internal standard solution, an immune enrichment solution, a dissociation solution, a leacheate, an eluent, a mobile phase 1 and a mobile phase 2. The complete IGF-1
protein can be fully automatically detected from top to bottom, and the sample treatment efficiency is remarkably improved; wherein the matrix liquid used for preparing the kit adopts human serum without endogenous IGF-1, so that the in-vivo environment can be simulated to the greatest extent, and the detection deviation caused by different matrixes is reduced; an eluent of the kit adopts a
formic acid-
hydrochloric acid mixed acid-
acetonitrile system, the
system can provide a mild
ionization environment through
formic acid, and
hydrochloric acid synergistically stabilizes the synergistic effect of the pH value of the solution, so that the
ion inhibition effect is reduced, the response
signal of IGF-1 is improved, and the
signal-to-
noise ratio of detection is further improved.