The invention discloses a method for measuring the sulfur-containing amino acid content of Astragali mushroom, and relates to the field of measurement of amino acid content of the Astragali mushroom.The method comprises the following steps: performing performic acid oxidation protection on the Astragali mushroom serving as a raw material, performing acid hydrolysis, performing ultrafiltration centrifugation, performing derivatization by taking 2,4-dinitrofluorobenzene as a derivatization reagent, performing gradient elution on the obtained derivative sample liquid through high performance liquid chromatography and by taking acetonitrile-water and sodium acetate as flowing phases, detecting under the wavelength of 360 nm, separating derivatives of cysteic acid and methionine sulfone, measuring the peak area of a cystine standard product and a methionine standard product under different concentration gradient, drawing a standard curve, and measuring the cystin content and the methioninecontent separately to obtain the sulfur-containing amino acid content. According to the method, the requirement on instruments is low, the reaction condition does not need to be strictly limited, thequantity of the needed samples is small, the samples are not damaged, single component can be analyzed, and the method is a measuring method which is efficient, accurate, simple, convenient and easyto operate and low in price.