The invention belongs to the technical field of detection methods, and provides a method for determining the content of
amino acid in chyme in digestive tracts of
livestock and poultry, which comprises the following steps: mixing a sample and a
peroxyformic acid solution, and reacting to obtain an oxidation
system; mixing the oxidation
system, a
hydrochloric acid solution and a chelating agent solution, and hydrolyzing to obtain a
hydrolysis system; mixing the
hydrolysis system and alkali liquor, then adjusting the pH value, and then mixing with acid liquor to obtain liquid to be detected; centrifuging the to-be-measured liquid, filtering, injecting a filtering system and the mixed
amino acid standard liquid into an automatic
amino acid analyzer, and measuring. According to the method, a certain amount of disodium
ethylene diamine tetraacetate is added to chelate
heavy metals in chyme in digestive tracts of
livestock and poultry, and meanwhile, the
heavy metals and redundant impurities are removed by adopting the steps of centrifuging, filtering and the like, so that the accuracy of amino acid detection is ensured, and the addicability of digestible
amino acid content in daily ration is better. The
hydrolysate is treated by adopting simple steps such as
pH regulation and
centrifugation, so that the operation process is simplified, and the operation error is reduced.