34 results about "Endospore" patented technology

The invention discloses a bacillus velezensis ZJ20 strain and liquid preparations thereof. The bacillus velezensis ZJ20 strain is preserved in China general microbiological culture collection center in Chaoyang district, Beijing, China under the number of CGMCC NO.5995 on April 13 2012. A microbial biocontrol agent, namely the novel bacillus velezensis ZJ20 strain, prepared by screening has endospores with high stress resistance and long shelf life, is highly environmentally adaptive and can be produced into commercial biological agents. Metabolites of the bacillus velezensis ZJ20 strain have high bactericidal power, have high colonization ability and fast speed on plants, and can occupy the surface of hybrid bamboo effectively to prevent dieback. Antibacterial protein (chitin with protein) produced by the strain is antibacterial, can promote growth of hybrid bamboo and various types of bamboo branches and leaves, and have a novel effect of recovering posterior production capacity.

The invention relates to a Microbial Product which can decompose the organic pollutant in farming waters rapidly and digestion the content of ammonia nitrogen and nitrite in water in time with using nitrobacteria, Bacillus endospores and other bacteria. The characteristic of this product was that the percent by weight before compound was Nitroso-bacteria 20%-40%, nitrifier 20-40%, Bacillus licheniformis 20%-60%. This invention using screening environments and adding concentration technology, at the same time combine directional concentration with fermentation to finish the process of enrich and enlarge the nitrifier by one step . This can reduce the culture time of nitrifier obviously, so that the using of nitrifier would from laboratory to industrialization. The ten times amount about Nitrobacteria than liquid product can complement and assort with high concentration Bacillus endospores to maintain the water with the best farming condition of rich, live, fecund and clear. This product can basically replaced the chemical medicine using in breed aquatics and produce the organic farm produce with green, safe and no antibiotic residue.

The present invention provides antibodies that bind to the endospore of the bacterium Clostridium difficile, methods of making such antibodies, and methods of using such antibodies, including methods of detecting C. difficile endospores.

The invention provides bactericidal composition, a preparation and an application of the bactericidal composition. Bactericidal active ingredients of the bactericidal composition mainly comprise Bacillus amyloliquefaciens and an antibiotic in the weight ratio being 10:(0.5-20), wherein the antibiotic is any one of kasugamycin, polyoxin and Zhongshengmycin, and the number of live endospores of the Bacillus amyloliquefaciens is 5-50 billion / g. The Bacillus amyloliquefaciens and the antibiotic are compounded to have a synergistic effect, and the control effect on diseases is better than that of single chemicals.

A method and device for detecting, differentiating from background and providing partial identification (i.e., classification) for biological particles found in aerosols or surface dust. The method is based on the phenomenon that luminescent excitation-emission (EEM) graphs of microorganisms obtained before and after perturbation by irradiation with ultraviolet light show characteristic patterns which differ according to the type of particle. For example, Bacillus endospores may be distinguished from vegetative bacteria, and gram positive vegetative bacteria may be distinguished from gram negative bacteria, and all these may be distinguished from many types of background particles, e.g. house dust, road dust, and pollen.

Provided are shelf-stable probiotic compositions comprising endospores of Bacillus with desirable characteristics such as acid resistance, high temperature tolerance, and high levels of phytase, α-amylase, cellulolytic and / or protease enzyme activities. Methods for their use, e.g. in food for animal and human consumption, are also provided, as are food products which comprise the probiotics and methods of making the same.

This invention discloses a mutated strain of wild type Bacillus mucilaginosus HSCUP-76-8 and a high-density fermentation method thereof. The mutated strain HSCUP-76-8 was assigned an Accession No. CGMCC No. 8481. A two-stage and regulated high-density fermentation method has been established for the production of HSCUP-76-8. In the first stage, parameters are controlled to reduce the viscosity of the fermentation broth and promote the growth of bacteria, thereby allowing the bacteria to reach an amount in the range of 2.0×109 cfu / mL-2.3×109 cfu / ml. In the second stage, nutritional factors and fermentation conditions are controlled to promote sporulation, thereby producing endospores in the range of 1.5×109 cfu / mL-2.0×109 cfu / ml. The fermentation cycle of the two-stage high-density fermentation is 32-48 hours.

The invention provides a method for regulating and controlling the grain size of microbial-induced deposited calcite. The method includes the following steps: 1) inoculating endospores of Bacillus mucilaginosus or endospores of photosynthetic bacteria or endospores of Bacillus pasteurii to corresponding culture mediums separately so as to obtain a concentrated bacterial solution of Bacillus mucilaginosus or a concentrated bacterial solution of photosynthetic bacteria or a concentrated bacterial solution of Bacillus pasteurii separately; 2) adding a calcium source and urea to the concentrated bacterial solution of Bacillus mucilaginosus or the concentrated bacterial solution of photosynthetic bacteria or the concentrated bacterial solution of Bacillus pasteurii obtained in the step 1), andperforming still standing; and 3) after production of precipitates, washing the precipitates, performing filtration and drying, performing drying until constant weight, and then analyzing the grain size so as to prepare the calcite with a grain size of 20-60 nm. The microbial method has the characteristics of high efficiency and environmental protection, the grain size of the calcite can be adjusted according to needs, the formed calcite mineral has stable properties, and effective capture and utilization of carbon dioxide can be achieved in the process, so that the greenhouse effect is sloweddown.

The invention discloses a preparation method of a slaughter house sewage disinfectant which belongs to the field of disinfectants. A finished product of the chicken house disinfectant is prepared by employing a chlorine dioxide aqueous agent, potassium ferrate and pure water as raw materials and blending the raw materials at a certain temperature. The preparation method is advantaged by being less in required devices, simple in production technology and low in cost. Production and utilization of the finished product is harmless to human body. The slaughter house sewage disinfectant has good killing effects on endospores, viruses, heterotrophic bacteria, iron bacteria, sulfate reducting bacteria, funguses and the like, and has multiple functions such as algae removing, mud peeling, anti-corrosion, anti-mildew, freshness-retaining, deodorization, chlorination, bleaching and the like. The finished product can fully satisfy a requirement of killing various bacteria and viruses and removing algae in sewage generated in a slaughter house, has a good disinfection effect and is low in cost, but the compound chicken house disinfectant is slightly poor in stability so that the compound chicken house disinfectant needs to instantly use after preparation.

The present invention relates to a water beverage containing spore-type lactic acid bacteria as a main component. Endogenous spore with protective effect is first produced inside lactic acid bacteria and then made to be in dormancy state and enter to water solution to prepare the beverage. In the production process and the drinking process, the lactic acid bacteria in dormancy state will not be damaged by different adverse factors (e.g. temperature, saliva, gastric acid and other digestive juices) in the digestive tracts and will be awaken in small intestinal tracts to perform the function of strengthening intestinal health.

The invention discloses an endospore stainer, which comprises a staining module and a supporting frame and also comprises a low-speed liquid staining funnel, a washing funnel and a waste liquid disk,wherein the low-speed liquid staining funnel and the washing funnel are installed on the top of the supporting frame; the waste liquid disk is installed on the bottom of the supporting frame; the staining module is used for a transition disk used for conducting heat in a staining and fixing operation, and a circulation slide frame installed on the surface of the transition disk; the low-speed liquid staining funnel and the washing funnel are both positioned above the circulation slide frame; the middle of a bottom disk is provided with an alcohol lamp groove used for fixing an alcohol lamp; and the alcohol lamp groove is positioned below the transition disk. The endospore stainer has a reasonable design structure, has bacteria staining universality, can simultaneously carry out staining operation processing on a plurality of slides, has a firm structure, has an anti-corrosion texture, is easy in cleaning, is durable in use and omits a great quantity of boring manual operation steps ina staining process. The endospore stainer is integrally convenient, clean and quick and can make the dosage of staining liquid clear, a situation that staining agent can be quickly dripped in an experiment process is guaranteed, and the endospore stainer can be conveniently cleaned after staining is finished.

The invention relates to a preparation method of a beverage containing sporogenous lactic bacterium. Endo-sporogenous lactic bacterium such as bacillus coagulans or enterococcus are used as main ingredients, the sporogenous lactic bacterium are screened and cultured and are positioned in aqueous solution in a dormant state, the dormant lactic bacterium are mixed with sterilized aqueous solution to prepare lactic bacterium water beverage, and then the lactic bacterium water beverage is canned in a packaging container. According to the preparation method of the beverage, the calories of the lactic bacterium beverage is reduced, the storage life of the lactic bacterium beverage is prolonged, the lactic bacterium can be utilized effectively, the manufacture cost is reduced, the lactic bacterium cannot be damaged before reaching the intestinal tract and thus the beverage is beneficial to the health of human body.

The invention relates to methods for inducing sporulation in Bacillus coagulans strains wherein excessive sporulation is induced by presence of certain nutrients and minerals up to a level of 109 spores / ml. The spores are tadpole-like endospores and the method discloses specific parameters of growth medium and incubation conditions to be used to induce sporulation in Bacillus coagulans strains.

The invention relates to the technical field of food analysis and detection and in particular relates to an inspection method of endospores and heat-resisting endospores. The method comprises the following steps: sampling: sampling according to a production technology before entering a UHT (ultra-high temperature) step; taking a first sample and a second sample respectively, wherein the first sample is a sample of endospores to be detected and the second sample is a sample of heat-resisting endospores to be detected; treating: treating the first sample at 78 to 82 DEG C for 8 to 12 min and treating the second sample at 98 to 102 DEG C for 8 to 12 min; diluting: diluting the treated first sample and second sample respectively according to the gradient; culturing: culturing the first sampleand counting and calculating the quantity of thermophile bacteria and mesophilic bacteria respectively; culturing the second sample and counting and calculating the quantity of the thermophile bacteria and the mesophilic bacteria respectively; judging a result: determining an acceptable range of the endospores and the heat-resisting endospores according to an AQL (Acceptable Quality Level). According to the method provided by the invention, the endospores and the heat-resisting endospores in a semi-finished product before the UHT step are inspected and the thoroughness of the UHT step is ensured, so that the quality of the semi-finished products and finished products is ensured.

The present invention provides a production technology of high-purity teicoplanin. The production technology comprises the following steps: 1) a slant culture medium, a seed culture medium and a fermentation culture medium are prepared; 2) fermentation liquid is prepared; 3) an eluent is prepared; and 4) a finished product is obtained. Mg2+ can improve activity of glucose oxidase of actinoplanaceae cells, Fe3+ can promote growth of live bacteria and formation of endospores, and the Mg2+ and Fe3+ produce an active center through a synergistic action, effectively conduct catalytic fermentation and increase yield of the teicoplanin.

A sporicidal composition has a moderately low pH and includes at least one oxidizing acid and the dissociation product of at least one inorganic oxidizing agent. Very high effective solute concentrations can enhance the efficacy of the composition. Embodiments of the composition can be applied to a surface and allowed to absorb into the endospore, ultimately killing at least some of those bacteria in mature endospore form. The surface being treated can be an inanimate surface, particularly a hard surface, or a medical device.

The invention discloses an aerobic denitrification bacterium ZJ-17 and application thereof. An aerobic denitrifying bacterium belongs to aeromonas hydrophila, is named as ZJ-17, is preserved in Chinese common microorganism culture preservation management center on May 27th, 2017 with the number being CGMCC NO.14206, and a sequence of 16S rDNA is as shown in a sequence table. The ZJ-17 bacterium isa non-fermenting Gram-negative bacterium and an obligate aerobe, has the growth temperature rage being 25 to 42 DEG C and the optimum temperature being 25 to 30 DEG C, cannot grow at 4 DEG C and cangrow at 42 DEG C. The bacterium body has the length being 1.5 to 5.0 mu m and the width being 0.5 to 1 mu m, is in a spherical rod shape or a linear shape, is arrayed in pair or a short chain shape, is uniflagellate on one end and has no endospores. The bacterial strain provided by the invention adopts multiple organic matters as a carbon source, the bacterial strain ZJ-17 is activated and cultured so as to have an excellent denitrification performance, and the nitrate nitrogen removal rate reaches up to 100 percent after the bacterium is applied to a sewage biological denitrification process.

A sporicidal composition has a moderately low pH and includes a glycosidase and the dissocia-tion product of at least one inorganic oxidizing agent. High effective solute concentrations can enhance the efficacy of the composition. The composition can be applied to a surface and allowed to absorb into an endospore, ultimately killing at least some of those bacteria in mature endospore form. Surfaces to be treated include inanimate (hard) surfaces via cleaning and medical devices such as endoscopes, typically via immersion.

The invention discloses a preparation method of a toilet disinfectant which belongs to the field of disinfectants. A finished product of the toilet disinfectant is prepared by employing sodium dichloroisocyanurate, geramine and pure water as raw materials and blending the raw materials at a certain temperature. The preparation method is advantaged by being less in required devices, simple in production technology and low in cost. Production and utilization of the finished product is harmless to human body. The toilet disinfectant can fully satisfy a requirement of toilet disinfection, can rapidly kill various pathogenic microorganisms such as endospores of bacteria, vegetative forms of bacteria, funguses and the like, has a special effect on hepatitis viruses, has completely killing effects on sulfate reducing bacteria, iron bacteria, funguses and the like in a circulating water system, has a good disinfecting effect and is low in cost.

A method is provided for the production of alcohol from trees, the method comprising a step of treating a subject tree with mother cell lyases formed through cytolysis associated with sporulation of spore-forming aerobic bacteria, thereby degrading said tree into a powdery state and obtaining a tree degradation product; a step of sterilizing said tree degradation product; a step of treating said sterilized tree degradation product with a koji fungus (Aspergillus oryzae) thereby carrying out a primary fermentation; a step of adding a yeast to the fermentation broth obtained by said primary fermentation thereby carrying out a secondary fermentation; and a step of filtering the fermentation broth obtained by said secondary fermentation, wherein said mother cell lyases are obtained by culturing said spore-forming aerobic bacteria, subjecting the resultant culture medium to a starvation state, thereby converting said bacteria into endospores, and removing impurities including said endosporic bacteria from said culture medium and wherein said spore-forming aerobic bacteria are MRE symbiotic bacteria.

A biologically-active aerosol spray composition for eliminating the persistent noxious odors commonly found in residential and commercial bathrooms comprises a mixture of endospores of one or more beneficial bacteria capable of digesting dried urine without producing noxious odors and a carrier liquid for the endospores, wherein the carrier liquid comprises acetone.

The invention discloses an alkalophilic bacillus NTT33C6. The preservation number of the strain is CCTCC M2016316, which has the ability to produce constitutive alkaline β-mannanase; the bacterium is rod-shaped, and the size is 0.5-0.65×2.6 ~3.4μm, flagella lateral, motile, endospore oval; aerobic type, colony round, smooth surface, with concentric circles. Bacillus alkalophilus NTT33C6 can be used for biological degumming of cannabis, with a degumming rate of over 70% in 6-8 hours; the degumming rate of over 80% in 6-8 hours for hemp that has not been removed from the husk; and the degumming waste liquid is easy to handle The combined use of biological degumming and chemical degumming provided by the present invention can produce lean hemp with a certain residual glue rate requirement according to production needs.

The invention discloses a multi-point sludge external reflux enhanced biological denitrification and phosphorus removal combination process, which belongs to the field of sewage treatment process of urban sewage plants, and divides the interior of biochemical pools into anaerobic pools, anoxic pools, and aerobic pools. pool, anoxic / aerobic transition pool, post-aerobic pool, the water inlet linear proportional distribution weir can directly adjust and distribute water to the anoxic pool, anoxic / aerobic transition pool according to a certain proportion, and the sludge in the secondary sedimentation tank is returned to In the biochemical tank, there is an external return sludge distribution weir outside the secondary settling tank, which is used to distribute the sludge in the secondary settling tank to the anaerobic tank, anoxic tank, aerobic tank, and anoxic / aerobic transition tank in a certain proportion. Add low-temperature-resistant endospore microbial value-added nutrient solution to the proportional distribution weir of the outward return sludge; use the anaerobic tank as a side-flow sludge anaerobic fermentation tank, and mechanically stir the sludge returned from the secondary sedimentation tank to the biochemical tank. Anaerobic fermentation in the side stream sludge anaerobic fermentation tank to increase the carbon source required for the subsequent biochemical process.