The invention discloses a preparation method of a slaughter house sewage disinfectant which belongs to the field of disinfectants. A finished product of the chicken house disinfectant is prepared by employing a chlorine dioxide aqueous agent, potassium ferrate and pure water as raw materials and blending the raw materials at a certain temperature. The preparation method is advantaged by being less in required devices, simple in production technology and low in cost. Production and utilization of the finished product is harmless to human body. The slaughter house sewage disinfectant has good killing effects on endospores, viruses, heterotrophic bacteria, iron bacteria, sulfate reducting bacteria, funguses and the like, and has multiple functions such as algae removing, mud peeling, anti-corrosion, anti-mildew, freshness-retaining, deodorization, chlorination, bleaching and the like. The finished product can fully satisfy a requirement of killing various bacteria and viruses and removing algae in sewage generated in a slaughter house, has a good disinfection effect and is low in cost, but the compound chicken house disinfectant is slightly poor in stability so that the compound chicken house disinfectant needs to instantly use after preparation.