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Bacillus velezensis ZJ20 strain and liquid preparations thereof

A ZJ20, Bacillus technology, applied in the direction of bacteria, microorganisms, microorganism-based methods, etc., to achieve the effects of long storage life, recovery of production capacity, and strong bactericidal power

Inactive Publication Date: 2013-12-18
SICHUAN AGRI UNIV
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Problems solved by technology

However, in recent years, the shoot blight of bract × green hybrid bamboo caused by the semi-known fungus Arthrospora genus Arthrospora has become one of the important diseases threatening the hybrid bamboo industry, seriously hindering the growth of bracing. The development of ×green hybrid bamboo even brings about devastating disasters, greatly reducing the application value of supporting ×green hybrid bamboo

Method used

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  • Bacillus velezensis ZJ20 strain and liquid preparations thereof

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Embodiment Construction

[0014] ①Level 1 slant seeds: make beef extract peptone slant medium (beef extract 7.0g, peptone 10g, NaCl 5g, agar 17g, water 1000mL) by conventional method, inoculate Bacillus Velez ZJ20 and culture at 26-28℃ for 24- 36 hours to make a first-class seed.

[0015] ②Second-level liquid seeds: the improved nutrient succulent culture medium (beef extract 7.0g, peptone 10g, NaCl 5g, bamboo leaf soaking solution 1000mL) after autoclaving, according to the ratio of 100mL liquid to 300mL triangular flask (oxygen control) Bottles, inoculated with slant seeds of Bacillus velei ZJ20 in a sterile state, inoculated 2 slant seeds per bottle, temperature 26-28°C, initial pH value 6.5, shaking culture at 120r / min for 36h, and made Bacillus velesi ZJ20 liquid seeds.

[0016] ③ Liquid fermentation: After the improved nutrient meat culture solution (beef extract 7.0g, peptone 10g, NaCl 5g, bamboo leaf soaking solution 1000mL) is autoclaved, it is bottled according to the proportion of 200mL liq...

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Abstract

The invention discloses a bacillus velezensis ZJ20 strain and liquid preparations thereof. The bacillus velezensis ZJ20 strain is preserved in China general microbiological culture collection center in Chaoyang district, Beijing, China under the number of CGMCC NO.5995 on April 13 2012. A microbial biocontrol agent, namely the novel bacillus velezensis ZJ20 strain, prepared by screening has endospores with high stress resistance and long shelf life, is highly environmentally adaptive and can be produced into commercial biological agents. Metabolites of the bacillus velezensis ZJ20 strain have high bactericidal power, have high colonization ability and fast speed on plants, and can occupy the surface of hybrid bamboo effectively to prevent dieback. Antibacterial protein (chitin with protein) produced by the strain is antibacterial, can promote growth of hybrid bamboo and various types of bamboo branches and leaves, and have a novel effect of recovering posterior production capacity.

Description

technical field [0001] The invention relates to a new bacterial strain ZJ20 of Bacillus velezensis and its liquid preparation. Background technique [0002] Support × green hybrid bamboo is one of the main economic bamboo species introduced and developed in Southwest China and other places, and plays a huge role in economic, ecological and social benefits. However, in recent years, the shoot blight of bract × green hybrid bamboo caused by the semi-known fungus Arthrospora genus Arthrospora has become one of the important diseases threatening the hybrid bamboo industry, which seriously hinders the development of bracing. The development of ×green hybrid bamboo even brings about devastating disasters, greatly reducing the application value of supporting ×green hybrid bamboo. At present, the prevention and control of this disease in forestry production mostly adopts chemical control, but because long-term application of chemical pesticides will not only increase the resistanc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 朱天辉李姝江梁漫谯天敏
Owner SICHUAN AGRI UNIV
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