A kind of alkalophilic bacillus ntt33c6 producing constitutive mannanase and its application

Abstract

The invention discloses an alkalophilic bacillus NTT33C6. The preservation number of the strain is CCTCC M2016316, which has the ability to produce constitutive alkaline β-mannanase; the bacterium is rod-shaped, and the size is 0.5-0.65×2.6 ~3.4μm, flagella lateral, motile, endospore oval; aerobic type, colony round, smooth surface, with concentric circles. Bacillus alkalophilus NTT33C6 can be used for biological degumming of cannabis, with a degumming rate of over 70% in 6-8 hours; the degumming rate of over 80% in 6-8 hours for hemp that has not been removed from the husk; and the degumming waste liquid is easy to handle The combined use of biological degumming and chemical degumming provided by the present invention can produce lean hemp with a certain residual glue rate requirement according to production needs.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an alkalophilic bacillus NTT33C6 producing constitutive mannanase, and at the same time relates to a method for separating and screening the alkalophilic bacillus NTT33C6, and also relates to the use of the strain in cannabis biological degumming and cannabis production use. Background technique [0002] Marijuana (Cannabis sativa L.) belongs to the family Moraceae (Maraceae) and belongs to the genus Cannabis. Annual erect herb, 1-4 meters high. Hemp has the biological characteristics of barren tolerance, strong stress resistance, wide adaptability, light-loving (especially short-day) and high photosynthetic efficiency. [0003] China has a long history of growing marijuana and using hemp products. Fragments of marijuana from the Shang Dynasty have been unearthed in Henan, my country, and hemp seeds unearthed in Linxi, Dongxiang, Gansu are also more than 5,00...

AI Technical Summary

Problems solved by technology

The main problems of the chemical degumming method: first, the process is long, consumes a lot of raw materials and energy consumption, resulting in high production costs; second, the use of high-concentration acid, alkali, oxidant, etc., causes great damage to the fiber and poor fiber quality , so it is difficult to meet the requirements of spinning high-count yarn; the third is that the COD of degumming sewage produced Cr (chemical oxygen demand) can be as high as 15000-25000mg / mL, and BOD 5 / COD Cr <0.3, belongs to very difficult sewage, in order to meet the discharge standard, it needs to be treated after a large amount of dilution, so the treatment cost is high, and it is difficult to meet the standard, so it will seriously pollute the environment (Wu Junnan, Hao Xinmin, Tang Zongliu, etc. Hemp fiber high temperature— Flash explosion combined degumming technology [J], Textile Journal, 2007, 28(11):76-80.)

[0012] But from the current point of view, many biological degumming methods cannot be applied to industrial production, mainly because the enzyme activity is too low, and the raw hemp after enzyme degumming still contains more colloids, and its effect is only equivalent to the pretreatment process of chemical degumming The effect of (Yu Chunhua, Feng Xinxing, Jia Changlan, etc. Hemp fiber high temperature-enzyme combined degumming technology [J]. Textile Journal, 2007, 28 (6): 79-82. Cai Xia, Xiong Heping, Yan Li, etc. Cannabis microorganisms— Steam Explosion Combined Degumming Technology[J]. Textile Journal, 2011,32(7):75-79.)

Therefore, until now, there is no example of the successful use of cannabis biological degumming method in production

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