The application is suitable for the field of analog
enzyme technology, and provides a light-controlled zein-based dual-
enzyme-activity analog
enzyme and a preparation method thereof.The preparation method comprises the following steps: under water bath conditions, dissolving zein in an alkaline
aqueous solution and incubating; adding a
hydroxylamine solution to the solution obtained in step S1 and continuing to react; and after the reaction is completed, obtaining the light-controlled zein-based dual-enzyme-activity analog enzyme.The application uses zein as a
raw material, is modified by
hydroxylamine, does not need any
metal component to participate, has light-controlled switchable dual-enzyme activity, exhibits oxidase-like activity under
visible light irradiation, presents
peroxidase-like activity in dark conditions, and can realize reversible switching of the two enzyme activities by regulating light, effectively overcomes the problems that traditional artificial analog enzymes depend on
metal ions, multiple enzyme activities coexist, interfere with each other and are difficult to accurately control, can be used for colorimetric detection of
total antioxidant capacity, can be used for colorimetric detection of glucose in
cascade reaction with
glucose oxidase, has the advantages of simple operation,
rapid response, high sensitivity, good selectivity, low cost and the like, and has a good application prospect in the fields of
food analysis,
clinical diagnosis and the like.