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163 results about "Food Analysis" patented technology

Measurement and evaluation of the components of substances to be taken as FOOD.

Rapid bacterial detection method based on upconversion fluorescence resonance energy transfer

The invention discloses a rapid bacterial detection method based on upconversion fluorescence resonance energy transfer, and belongs to the technical field of bacterial detection. The method includes the steps that the gold nanoparticle surface is modified with nucleic acid aptamers capable of specifically recognizing a target bacterium, the rare earth upconversion fluorescence particle surface is modified with cDNA paired with the aptamers complementarily, after the two kinds of nanoparticles are mixed, the distance between the two kinds of particles is reduced due to base complementary pairing of two nucleotide sequences, and therefore fluorescence of upconversion fluorescence particles is quenched by gold nanoparticles. When a target object is added, the target object and cDNA are competitively combined with the aptamers to recover fluorescence of upconversion fluorescence particles. The target bacterium is measured quantitatively according to different fluorescence recovery strength. The method is high in sensitivity and specificity, easy to operate, various target objects can be determined by changing aptamer sequences, and the rapid bacterial detection method is of great significance in environment monitoring and food analysis.
Owner:XI AN JIAOTONG UNIV +1

MOFs material-based series molecular sieve and preparation method and application method thereof in complex sample purification and separation analysis

The invention discloses a metal-organic frameworks (MOFs) material-based series molecular sieve and a preparation method and an application method thereof in complex sample purification and separation analysis, and relates to the technical field of chromatography application and the field of solid phase micro extraction. The series molecular sieve comprises an MOFs material-based solid phase micro extraction device and an MOFs material-based capillary gas phase chromatographic column; the MOFs material-based solid phase micro extraction device comprises a section of stainless steel fiber extraction head, the surface of which is coated with an MOFs material; and the MOFs material-based capillary gas phase chromatographic column comprises a section of capillary, the inner wall of which is uniformly coated with a layer of MOFs material. High-selectivity purification, efficient separation and specificity detection and measurement of a complex sample are realized by combined use of the solid phase micro extraction device coated with the MOFs material and the gas phase chromatographic column coated with the MOFs material. The series molecular sieve is favorable for large-scale popularization and application, and has broad application prospect in the fields of analytical chemistry, particularly environmental analysis, food analysis and the like.
Owner:NANKAI UNIV

Up-conversion fluorescent aptamer-based side stream test paper detection method

The invention discloses an up-conversion fluorescent aptamer-based side stream test paper detection method, and belongs to the technical fields of environment detection and food safety detection. A nucleic acid aptamer capable of specifically identifying target bacteria is modified on the surface of each nano-gold particle; cDNA which is in complementary pairing with the aptamer is modified on thesurface of each rare earth up-conversion fluorescent particle; and after the two kinds of nanoparticles are mixed, the basic groups of the two segments of nucleic acid sequences are in complementarypairing and the distance of the two kinds of particles are pulled closely, so that fluorescence of the upper-conversion fluorescent particles is quenched. After a target object is added, the target object can be bound with the aptamer with cDNA competitiveness, so that fluorescence recovery of the up-conversion nanoparticles is caused. According to different fluorescence recovery intensity, quantitative measurement of the target bacteria can be realized. The method provided by the invention is high in sensitivity, high in specificity and simple and convenient in operation, various target objects can be measured through change of the aptamer sequence, and very import significance in the aspects of environment monitoring, food analysis and the like is achieved.
Owner:XI AN JIAOTONG UNIV

Smell detection system based on odor evaporation characteristic spectrum and system thereof

The invention belongs to the odor detection technology, relates to the technical field of odor sample processing and smell and discloses a smell detection system based on an odor evaporation characteristic spectrum and a system thereof. The artificial smell technology has been widely applied in the field of detection of various types of odor, and the smell detection capability can be greatly improved through improving the volume of odor information obtained by a single sensor. The invention describes evaporation characteristics of a liquid sample based on the principles that the components of the liquid sample has different evaporation characteristics, and odor generated in evaporation at different time has different gas components and contents thereof during evaporation of the liquid sample of a finite volume, and a specific type of gas sensor responds to odor generated in evaporation at different time to obtain sensor signals changing along with the time, i.e. the odor evaporation characteristic spectrum. Therefore, the invention increases odor information obtained by the gas sensor, improves the accuracy of odor analysis and has great market application value in food analysis, security testing, medical treatment, public health and other aspects.
Owner:HUAZHONG UNIV OF SCI & TECH

A method and device for rapid and automatic determination of melamine content in dairy products

The invention discloses a rapid and automatic determination method and device for tripolycyanamide content in dairy products, belonging to the field of food analysis. The method comprises the following steps that: in a unit (A) under an operating procedure, a first step, a multifunctional valve is in a state I, a pump A rotates, a pump B stops, a first sample flows into a column 2 for enriching tripolycyanamide, and the eluent flows into a column 1 for regenerating tripolycyanamide; a second step, the pump A stops, the pump B rotates, the cleanout fluid flows into the column 2 for cleaning the impurities therein; a third step, the multifunctional valve is switched to a state II, eluent flows into the column 2 for eluting and regenerating tripolycyanamide; the tripolycyanamide in the column 2 is substituted by the eluent and directly enters into a sampling ring of a unit (B) in the form of a sample plug, so as to perform volume quantification, injection, re-separation and detection, and a response signal is processed by a computer; and meanwhile, a second sample enters into the column 1 for enrichment, and the process of the column 2 is repeated. The method and the device are high in automation degree and excellent in reproducibility, and can analyze about 30 samples per hour; and the unit (A) in the invention can be used with various flow injection analysis systems.
Owner:SICHUAN UNIV
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