Biosensor for detecting Hx(hypoxanthine) in aquatic products

A biosensor, hypoxanthine technology, applied in the field of food analysis, can solve the problems of complicated synthesis, complex sensor preparation, long detection period, etc., and achieve the effects of good selectivity, simple and fast preparation process, and low detection cost

Inactive Publication Date: 2019-06-07
FUZHOU UNIV
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Problems solved by technology

Hypoxanthine Hx is often used as an indicator to reflect the freshness of aquatic products. In recent years, various biosensors, including electrochemical fluorescence and optical sensors, have been developed for the determination of Hx, because biosensors have ad...

Method used

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  • Biosensor for detecting Hx(hypoxanthine) in aquatic products
  • Biosensor for detecting Hx(hypoxanthine) in aquatic products
  • Biosensor for detecting Hx(hypoxanthine) in aquatic products

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Experimental program
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Embodiment 1

[0023] 1 mL of 16 mM chloroplatinic acid aqueous solution and 1 mL of 40 mM trisodium citrate solution were added to 38 mL of water and stirred at room temperature for 30 minutes. Then, add 200 μL of 50 mM sodium borohydride solution to the mixture. After standing still for 1 h, a brown suspension of Pt NPs was obtained, and the average particle size of the as-prepared Pt NPs was about 2.4 nm. All glassware used in the above process was soaked in aqua regia, rinsed thoroughly with ultrapure water, and air-dried.

[0024] figure 1 and figure 2 Shown is the verification of the feasibility of the sensor, Pt NPs alone have no fluorescence absorption peaks, when adding OPD and H 2 o 2 Then the solution has a fluorescence absorption peak at 580nm, and when H 2 o 2 When the concentration is high, the intensity of the absorption peak decreases. figure 2 In the solution, only when Hx and XOD are added at the same time, the fluorescence intensity of the solution will show a sig...

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Abstract

The invention relates to a biosensor for detecting Hx (hypoxanthine) in aquatic products and application thereof, and belongs to the field of food analysis. A fluorescence biosensor is built on the basis of the catalysis activity of Pt NPs (platinum nanoparticles), and is used for detecting the content of Hx in the aquatic products. A provided sensing system consists of XOD (xanthine oxidase), OPD(o-phenylenediamine) and Pt NPs; Hx reacts with oxygen under the existence of XOD to produce uric acid and H2O2; the Pt NPs can catalyze the oxidization reaction of the OPD and the H2O2 to produce 2,3-phenazine diamine giving out yellow fluorescence; and a fast, simple, convenient and sensitive aquatic product freshness degree detection method is provided. The novel detection method has the characteristics that the preparation of the Pt NPs is simple; the acquisition is easy; the simple and convenient analysis on the aquatic product freshness degree can be realized; the operation is simple; and the sensitivity and the selectivity are high. The popularization and the use are easy.

Description

technical field [0001] The invention relates to a biosensor for detecting hypoxanthine in aquatic products to detect the freshness of aquatic products, belonging to the field of food analysis. Background technique [0002] The freshness of aquatic products is an important indicator to evaluate its quality. Due to their nutrient-rich nature, aquatic products are prone to spoilage during storage and sale under the joint action of enzymes and microorganisms. However, the freshness assessment of aquatic products is complicated because the spoilage of meat involves different microbial, physicochemical and biochemical reactions. Traditionally, there are two approaches to assess the freshness of aquatic products: (1) sensory testing with sensory characteristics controlled by experts, and (2) chemical and biochemical determination of the concentration of target biological indicators. Although the former method has proven to be fast, it is expensive and sometimes unreliable because...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 翁祖铨陈家敏鲁奕严芬黄达
Owner FUZHOU UNIV
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