67 results about "Hypoxanthine synthesis" patented technology

The invention relates to a freshness detecting method used for royal jelly. In the specific detecting method, the contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), single phosphoric acid adenosine (AMP), inosinic acid (IMP), hypoxanthine riboside (HxR), hypoxanthine (Hx), adenine and adenosine of the royal jelly are quantitatively determined through methods of liquid chromatography or ultraviolet spectrometry, and the like; the ratio (F value) between the accumulation amount of adenine, adenosine, HxR and Hx and the sum of the qualities of the eight substances (degradation products of ATP and nucleic acid)is calculated; the F value of the royal jelly is used for detecting the freshness of the royal jelly and judging the quality of the royal jelly. Compared with a present royal jelly quality detecting method, the freshness detecting method can judge the quality and the freshness of the royal jelly more accurately and sensitively, can improve the quality standard of the present royal jelly and effectively monitor the quality of the royal jelly.

A bisflavone medicine for preventing and treating gout, preventing the xanthine and hypoxanthine from being oxidized to become uric acid, and relaxing the hyperuricemia and its secondary diseases is prepared from one or more of bisflavone compounds and the auxiliaries.

The invention discloses a Chinese hamster ovary culture medium as well as a preparation method and application thereof. The culture medium comprises ascorbic acid, pyridoxine hydrochloride, vitamin B12, nicotinamide, riboflavin, thiamine chloride, choline chloride, folic acid, calcium pantothenate, sodium pyruvate, reduced glutathione, inositol, biotin, hypoxanthine, putrescine, lipoic acid, linoleic acid, thymine, glucose, HEPES (2-hydroxyethyl) buffer solution, dextran sulphate, Pluronic-F68, various amino acids and salts thereof and various inorganic salts. The culture medium has low cost, and the component does not contain animal origin ingredients, does not contain protein and has definite chemical ingredients. All ingredients are cooperated and blended, and the Chinese hamster ovary culture medium can satisfy the basic growth requirement of cells, and can effectively regulate, transfer and control the culture of cells so as to realize good high-density cell culture. Compared with the culture effect of the existing culture medium, the culture effect of culture medium disclosed in the invention is at least equivalent to even superior to the existing culture medium.

The invention relates to a recombinant bacterium for generating inosine. Compared with an original strain, the recombinant bacterium has the weakened phosphopentose mutase activityor inactivated transphosphorylase. The original strain is a strain capable of accumulating inosine. The invention finds an improving target for increasing the fermenting yield of inosine and establishing the corresponding recombinant bacterium. A test proves that the inosine yield of the recombinant bacterium can be obviously increased and the accumulation volume of the side product hypoxanthine can be reduced.

Compounds which are active against viruses have the following Formulas:

wherein B is a purine or pyrimidine heterocyclic ring and is preferably selected from the group consisting of 6-aminopurine (adenine), 2,6-diaminopurine, 2-amino-6-azidopurine, 2-amino-6-cyclopropylaminopurine, 6-hydroxypurine (hypoxanthine), 2-amino-6-halo substituted purines, 2-amino-6-alkoxy substituted purines, 2-amino-6-hydroxypurine (guanine), 3-deazapurines, 7-deaza-purines, 8-azapurines, cytosine, 5-halo substituted cytosines, 5-alkyl substituted cytosines, thymine, uracil and 6-azapyrimidines; X is 0; and, R₁ and R₂ are alkyl or aryl groups. The compounds of the present invention also include the R- and S-enantiomers of the above compounds. The R,X and/or R₂X can also be amino acid residues with X as NH.

The invention discloses a building method of an acute hyperuricemia renal damage mouse model. The method comprises the following steps of experiment mice are subjected to molding experiment for many days by using xanthine, ethambutol and oteracil potassium as molding agents; obtaining the acute hyperuricemia renal damage mouse model; observing the body weight, the serum uric acid level and the renal function change of the experiment mice and the kidney pathological change; the renal damage effect of the molding agent on the experiment mice is analyzed. The mice are used as model animals; the serum uric acid level of the mice is equivalent to the uric acid level of a hyperuricemia patient; the serum uric acid level is stable and can easily reappear; meanwhile, the combined effect of uric acid precursors of hypoxanthine is used; the uric acid excreted substances of ethambutol and uricase inhibitors of oteracil potassium are reduced; the acute hyperuricemia renal damage mouse model is built; the molding time is short; the model conforms to the clinical characteristics of human body hyperuricemia renal damage, and is applicable to screening hyperuricemia renal damage resisting medicine.

The invention discloses a synthesis method of 6-chloropurine as shown in formula II. The method comprises the following steps that bis(trichloromethyl)carbonate and hypoxanthine shown in formula I are taken as raw materials to react for 4 to 10 hours in organic solvent under the action of organic amine catalyst at a temperature of between 0 and 150 DEG C; a filter mass is taken out after filtration to obtain 6-chloropurine hydrochlorate and then is dissolved in ice water; the pH value of the solution is adjusted between 7 and 9 by alkali to precipitate the 6-chloropurine, and the filtrate is eliminated through filtration so as to obtain the 6-chloropurine after drying; and the ratio of the reactants, namely the hypoxanthine: the bis(trichloromethyl)carbonate: the organic amine catalyst is equal to 1:0.33-0.66:0.1-1. The synthesis method has the advantages of simple and safe operation, higher reaction yield, low production cost and simple post treatment; moreover, the method technically eliminates the problems of the prior method including great hidden danger, serious three-waste pollution, and the like, thereby having higher practical value.

The invention discloses a xanthine dehydrogenase intercepting body and an application thereof. The protein comprises xanthine dehydrogenase intercepting body small subunit, xanthine dehydrogenase intercepting body intermediate subunit, and xanthine dehydrogenase intercepting body large subunit. The affinity of xanthine dehydrogenase intercepting body is increased by 19% by comparing a wild substrate (xanthine), the conversion number is increased by 115%, the catalysis efficiency is increased by 166%, and the temperature toleration is increased by 11 DEG C. The xanthine dehydrogenase intercepting body is in favor of developing the novel application field by combining with other enzymes, and is suitable for sample detection for detecting xanthine and hypoxanthine, the xanthine dehydrogenase intercepting body can be used for detecting inorganic phosphoric acid by combining with PNP enzyme, can be used for detecting adenosine deaminase by combining with PNP, XOD and POD enzymes and detecting 5'-nucleotidase, and is suitable for industrial application. The xanthine dehydrogenase intercepting body is in favor of increasing enzyme catalysis efficiency and reducing cost, and is in favor of industrial application.

The invention discloses a culture medium for enhancing ectogenesis of ox preantral follicle and application thereof. A method for preparing the culture medium for facilitating ectogenesis of isolated mammal preantral follicle comprises the following step of: mixing insulin, transferrin, sodium selenite, sodium pyruvate, glutamine, hypoxanthine, blood serum, follicle-stimulating hormone, luteotropin, estrogen, a PTEN (Pentaerythritol Tetranitrate) inhibitor with an alpha-MEN culture medium to obtain the culture medium. As proved by an experiment, ox preantral follicle is firstly separated from cortex ovarii, the obtained follicle is cultured in vitro in a culture medium and a control culture medium provided by the invention, and the diameter accretion and the cavitation rate of the follicle are observed. A result indicates that the cavitation rate and the diameter accretion value of the preantral follicle obtained by culturing with the culture medium provided by the invention are higher than those of the preantral follicle obtained with the control culture medium, and a necessary culturing system is provided for further excavation of a female reproductive resource.

The invention relates to a biosensor for detecting Hx (hypoxanthine) in aquatic products and application thereof, and belongs to the field of food analysis. A fluorescence biosensor is built on the basis of the catalysis activity of Pt NPs (platinum nanoparticles), and is used for detecting the content of Hx in the aquatic products. A provided sensing system consists of XOD (xanthine oxidase), OPD(o-phenylenediamine) and Pt NPs; Hx reacts with oxygen under the existence of XOD to produce uric acid and H2O2; the Pt NPs can catalyze the oxidization reaction of the OPD and the H2O2 to produce 2,3-phenazine diamine giving out yellow fluorescence; and a fast, simple, convenient and sensitive aquatic product freshness degree detection method is provided. The novel detection method has the characteristics that the preparation of the Pt NPs is simple; the acquisition is easy; the simple and convenient analysis on the aquatic product freshness degree can be realized; the operation is simple; and the sensitivity and the selectivity are high. The popularization and the use are easy.

The invention discloses a nucleoside and base group type substance separation method and application thereof. A high performance liquid chromatography is used for separating nucleoside and base grouptype substances; a chromatographic column in the high performance liquid chromatography is an amino chromatographic column of a triple-bond bonded acylamino bonded phase; mobile phases in the high performance liquid chromatography are a saline water containing solution and a polar organic solvent; the nucleoside and base group type substances are adenosine, cytidine, guanosine, adenine, hypoxanthine and guanine. The separation method has the advantages that the operation is simple; six kinds of nucleoside and base group type substances can be completely separated; the peak shape is good; the interference by other organic impurities is avoided; the sensitivity is high; simplicity and high speed are realized.

The invention relates to a working electrode for detecting hypoxanthine and xanthine, a preparation method of the working electrode, and an enzyme biosensor system using the working electrode; the preparation method of the working electrode is simple, the working electrode, a reference electrode, a comparison electrode and a electrochemical workstation form the enzyme biosensor system, the enzymebiosensor system can be used to detect the hypoxanthine and the xanthine, is higher in accuracy, can detect a low-concentration substrate, and is relatively highly sensitive to the changes of concentrations of the hypoxanthine and the xanthine, the detection range is relatively wide, the detection results of concentrations of the hypoxanthine and the xanthine are efficiently rapidly obtained, thestability of the enzyme biosensor system is very good, and the enzyme biosensor system can be repeatedly used, and still keeps the sensitive detection of the substrate after being used for multiple times.

The invention relates to a purine nucleosidase participating in purine-nucleoside-initiated anabolism of purine such as adenine, guanine, hypoxanthine, xanthine, and the like. The purine nucleosidase is obtained from a Bailing production fungus which is cordyceps sinensis hirsutella sinensis. The invention also relates to a purine nucleosidase coding gene, and an application of the purine nucleosidase. The amino acid sequence of the purine nucleosidase is represented as SEQ ID No.1. The nucleotide sequence of the coding gene is represented as SEQ ID No.2. From the respective of the principle, the invention makes detailed researches in the metabolic pathway in purine synthesis of purine nucleoside. The cloned DNA of the nucleotide sequence provided by the invention can be transferred into engineering fungus through methods of transduction, transformation, and conjugation transferring. Through the regulation upon the expression of biosynthesis gene of purine, high-expression is provided for host purine, such that an effective way for increasing purine yield is provided. Therefore, the invention has important application prospect.

A method for constructing a characteristic fingerprint spectrum of Pheretim or processed products thereof according to the invention, comprising the following steps of: (1) preparing a test product solution: crushing Pheretim raw product or processed products thereof, adding a NaCl solution, carrying out ultrasonic extraction, centrifuging, taking supernate, diluting to volume, and filtering, so as to obtain a test product solution; (2) preparing a control substance solution: taking hypoxanthine, inosine and uracil control substance, adding the NaCl solution and dissolving to obtain a controlsubstance solution; and (3) carrying out high performance liquid chromatograph detection, so as to construct the characteristic fingerprint spectrum of the Pheretim raw product or processed products thereof. According to the characteristic fingerprint spectrum of the Pheretim or the processed products thereof, construction method therefor and application thereof, hypoxanthine, inosine and uracil are adopted as control substance solution and a main detection index, so that hybrid peaks in the elution process can be effectively detected, and the Pheretim raw product and processed products can beaccurately calculated. The method disclosed by the invention has the advantages of simple operation, stability and high repeatability. According to the method, more complete quality evaluation can becarried out on the Pheretim and the processed products thereof, and the Pheretim and the processed products thereof can be quickly and accurately identified.

The present disclosure describes compositions for preparing a myofiber or myotube from a skeletal muscle stem cell or progenitor cell comprising a carnitine and/or a derivative thereof, a fatty acid a steroid and combinations thereof. Preferred embodiment comprises of 0,1 mM L-carnitine, 0.2 mM 9-cis-linoleic acid and 10 mM dihydrotestosterone. Also disclosed is a composition for inducing expansion of skeletal muscle stem cells or progenitor cells comprising a fibroblast growth factor agonist, a Notch signalling agonist, a nucleic acid, and combinations thereof. Preferred embodiment comprises 20 ng/ml basic fibroblast growth factor (bFGF), 50 [mu]g/ml [Delta]-like ligand 1 (DLL1), 10 mM hypoxanthine and 1.6 mM thymidine.

The invention discloses a 6-benayl aminopurine synthetic method. The method takes hypoxanthine as a raw material, thionyl chloride is taken as a solvent, phosgene or diphosgene or triphosgene is takenas a chloride agent, 4-dimethylaminopyridine or dialkyl aminopyridine is taken as a catalyst for a reaction, after the reaction is completed, 6-chloropurine is obtained, distilled thionyl chloride and phosgene dissolved in thionyl chloride are completely used for a next reaction, and then the obtained 6-chloropurine and benzylamine are subjected to the reaction to synthesize 6-benayl aminopurineunder existence of triethylamine. During a reaction process, the used chloride agent solid triphosgene is completely decomposed to the phosgene dissolved in the solvent under existence of the catalyst4-dimethylaminopyridine, and no solid constituent is residual.

The invention relates to the field of quality control of fresh meat, and mainly relates to a high-performance liquid chromatography method and an application for simultaneous determination of varioustaste nucleotides in fresh meat. The invention discloses a high-performance liquid chromatography method for simultaneous determination of various taste nucleotides in fresh meat, comprising the following steps: (1) pretreating a sample; (2) determining the content of each taste nucleotide in the sample to be tested by high-performance liquid chromatography method, separating and analyzing the contents of inosinic acid (IMP), adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine (I), and hypoxanthine (Hx) in meat sample by the high-performance liquidchromatography. The invention further discloses the application of the high-performance liquid chromatography detecting method in assessing the freshness of fresh meat. The invention has important significance for the development of the chilled meat market, can provide consumers with a reference for evaluating the freshness of fresh meat, and can provide production guidance for the chilled meat developer, which has strong theoretical value and practical significance.

The invention provides application of hypoxanthine, hypoxanthine riboside, xanthine, xanthosine or any combination thereof in preparation of medicines for treating tumors, and belongs to the technical field of medicines. The invention further comprises application of a combination of the hypoxanthine, the hypoxanthine riboside, the xanthine, the xanthosine or any combination of the hypoxanthine, the hypoxanthine riboside, the xanthine and the xanthosine and one or more of a human basic group, nucleoside or deoxynucleoside. The hypoxanthine, the hypoxanthine riboside, the xanthine, the xanthosine or any combination thereof can be applied to chemical treatment of malignant tumors of liver cancer, lung cancer, esophagus cancer, gastric cancer, colon cancer, pancreatic cancer, prostate cancer, leukemia, promyelocytic leukemia, breast cancer, ovarian cancer, cervical cancer or T-cell lymphoma and the like, has an obvious antitumor effect, is free of myelosuppression or an important organ damage at a normal dosage, and has the antitumor effects of high efficiency, broad spectrum and low toxicity.

The invention discloses a kit for determination of 5'-nucleotidase and a preparation method thereof, wherein the kit includes double-liquid components of a reagent R1 and a reagent R2 which are independent and includes the components and the corresponding content: the reagent R1 including a Good's buffer liquid, 5'-hypoxanthine nucleotide, xanthine oxidase, purine nucleotide phosphorylase, sodium azide, and a solvent being purified water; and the reagent R2 including peroxidase, 4-aminoantipyrine, 3-hydroxy-2,4,6-tribromobenzoic acid, sodium azide, and a solvent being purified water. The preparation method includes the steps: according to the component content, preparing the reagents; mixing a to-be-tested sample with the reagent R1 and the reagent R2, and carrying out full reaction; determining the absorbance difference value after the reaction with a fully automatic biochemical analyzer; and calculating the concentration of D-3-hydroxybutyrate in the sample according to the absorbance change value. The kit has the advantages of high accuracy and the like.

The invention discloses an intracellular purine electrochemical-detection method based on enzyme catalysis, which relates to an intracellular purine base detection method and aims at solving the problem in the prior art that the content of simple substances of purine in a metabolism process of intracellular nucleotide cannot be obtained. The electrochemical-detection method comprises the following steps: I. drawing standard curves, i.e., obtaining electrochemical signals by an electrochemical detector, calculating peak areas and drawing the standard curve of each standard substance; II. electrochemically detecting lysate twice, i.e., performing primary electrochemical detection on the lysate of a to-be-detected cell to obtain a xanthine-guanine mixed signal peak and an adenine-hypoxanthine mixed signal peak; adding a xanthine oxidase solution and performing second time of electrochemical detection to obtain a guanine signal peak and an adenine signal peak; III. calculating the content of single purine, i.e., by combining the standard curves with the result obtained by subtracting the peak area obtained before adding xanthine oxidase from the peak area obtained after adding the xanthine oxidase, calculating the content of each purine base. The electrochemical-detection method can be used in the field of cell detection.

The invention belongs to the field of medicinal chemistry, and in particular discloses a method for industrially preparing 6-purinethol. The method for industrially preparing the 6-purinethol is characterized by comprising the following steps of: heating and synthesizing phosphorus pentasulfide, anisole and hypoxanthine serving as raw materials in a container to obtain the 6-purinethol; and recycling a solid matter at an end point when the hypoxanthine disappears, and recrystallizing the solid matter to obtain the 6-purinethol. The method has the advantages of simple process, high yield, low cost, and suitability for industrial production.

The invention discloses a human synovial fluid mesenchymal stem cell culture medium. A formula of the human synovial fluid mesenchymal stem cell culture medium comprises the following ingredients: 9%to 11% of fetal calf serum, 12% to 15% of penicillin, 4% to 7% of streptomycin, 2% to 6% of amino acid, 3% to 5% of glucose, 4% to 6% of anhydrous calcium chloride, 1% to 4% of leucine, 5% to 9% of potassium chloride, 1% to 3% of hypoxanthine, 2% to 4% of thymidine, 3% to 5% of riboflavin, 2% to 5% of phenylalanine, 3% to 8% of fluorescence antibody and the balance of water. The invention furtherdiscloses a preparation method of the human synovial fluid mesenchymal stem cell culture medium. The preparation method comprises the following steps: S1, quantitatively weighing; S2, pouring the culture medium; S3, cleaning stem cells; S4, observing and culturing; S5, dyeing and marking. By means of the prepared human synovial fluid mesenchymal stem cell culture medium disclosed by the invention,the stem cells are visually in a spindle shape under a microscope, colony is formed, and the appearance characteristic of the synovial fluid mesenchymal stem cells can be met.

The invention discloses a siniperca chuatsi feed phagostimulant and a preparation method thereof. The siniperca chuatsi feed phagostimulant added to each kilogram of feed is prepared from the following raw materials by weight: 2.0g of animal liver extract, 0.2-0.5g of dimethyl-beta-propiothetin, 0.5-2.0g of trimetlylamine oxide, 0.2-0.5g of L-glycine, 20-25g of hypoxanthine nucleotide, 0.5-3.0g ofbetaine, 10-15g of L-alpha-alanine and 10-15g of cuttlefish viscera powder. The preparation method of the phagostimulant is as follows: taking the betaine, sequentially adding the animal liver extract, the dimethyl-beta-propiothetin, the trimetlylamine oxide, the L-glycine, the hypoxanthine nucleotide, the alanine and the cuttlefish viscera powder, conducting full uniform mixing once finishing adding one raw material, and finally conducting full uniform mixing. The phagostimulant is reasonable in formula, can improve the feeding rate, feeding-taming survival rate and weight gain rate of siniperca chuatsi, and can reduce feed coefficient. The preparation method of the phagostimulant is simple, and is convenient to operate and low in preparation cost.

The invention is about the measuring method of Inorganic Phosphates and its diagnosis reagent box. Producing hypoxanthine by reacting nucleoside phosphorylase with carnine under the existence of Inorganic Phosphates in the sample of plasma, serum and so on, then producing urate and hydroperoxide by reactinghypoxanthine withxanthine oxidase, and then reacting bimolecular hydroperoxide with peroxidaseand oxidating the colorless reduced chromogen combination to quinoneimine chromogen or indamide chromogen dyer with color, testing the variation of dominant wave-length400ú¡600nmabsorbance during the reaction and finally measuring the content of Inorganic Phosphates . This method has high specificity and would not be contaminated by material of internal and exogenous sources, and the result is precise and accurate. Using this method can realize the fast testing in common ultraviolet/ visible light analyzer or semiautomatic/automatic analyzer and doesní»t require special or additional apparatus, so the cost is low. Thus, this method can be easily promoted and applied in the whole industry.

The invention discloses a human cell culture medium which comprises amino acids, vitamins, inorganic salts, hypoxanthine, thymidine, glucose, sodium pyruvate, linoleic acid, lipoic acid, putrescine and 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid. Due to improvement on components of the culture medium, according to the growth and metabolism characteristics of a human cell line, components such as pyridoxine hydrochloride, copper sulfate, ferrous sulfate, zinc sulfate, magnesium chloride, disodium hydrogen phosphate, sodium hypoxanthine, linoleic acid, lipoic acid, putrescine, thymidine and the like are increased, a microenvironment for growth of cells in the human body is met, and synthesis of human cell bio-enzymes can be promoted. The concentration of each component in the culture medium is optimized, the instability brought by a novel component is reduced, biological characteristics of the cultured cells are well maintained, and usage of serum during cell culture can be reduced or avoided. According to the test, the culture medium can be suitable for adherent culture and suspension culture and is applicable to the culturing of most of the human cells.