Unlock instant, AI-driven research and patent intelligence for your innovation.
Kit for determination of 5'-nucleotidase and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A nucleotidase and kit technology, applied in the fields of medicine and biochemistry, can solve the problems of complex operation, isotope pollution, and low accuracy
Inactive Publication Date: 2016-11-09
ANHUI IPROCOM BIOTECH CO LTD
View PDF3 Cites 3 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] The technical problem to be solved by the present invention is to provide a test kit for measuring 5'-nucleotidase and its preparation method in order to overcome the defects of complex operation, low accuracy and isotopepollution in the prior art
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0062] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein
[0110] (c) Reaction time: 8 minutes, among them, the incubation time is 5 minutes, measure and read the absorbance A1 immediately after adding the reagent R2, read the absorbance A2 after 3 minu...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a kit for determination of 5'-nucleotidase and a preparation method thereof, wherein the kit includes double-liquid components of a reagent R1 and a reagent R2 which are independent and includes the components and the corresponding content: the reagent R1 including a Good's buffer liquid, 5'-hypoxanthinenucleotide, xanthine oxidase, purinenucleotide phosphorylase, sodiumazide, and a solvent being purified water; and the reagent R2 including peroxidase, 4-aminoantipyrine, 3-hydroxy-2,4,6-tribromobenzoic acid, sodiumazide, and a solvent being purified water. The preparation method includes the steps: according to the component content, preparing the reagents; mixing a to-be-tested sample with the reagent R1 and the reagent R2, and carrying out full reaction; determining the absorbance difference value after the reaction with a fully automatic biochemical analyzer; and calculating the concentration of D-3-hydroxybutyrate in the sample according to the absorbance change value. The kit has the advantages of high accuracy and the like.
Description
technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring 5'-nucleotidase and a preparation method thereof. Background technique [0002] 5'-nucleotidase (5'-NT), the full name is 5'-ribonucleotide phosphohydrolase. 5'-Nucleotidase is a hydrolase with low substrate specificity and can act on a variety of nucleotides. Mainly involved in the catabolism of nucleic acids, can specifically catalyze nucleoside 5'-nucleotidase and inosine nucleotides, 5'-nucleotidase in the movement of the human body, cell growth and development, fibrin synthesis, improve Epidermal or endothelial shielding function, recirculation and adhesion of neurotransmitting lymphocytes, and immune response all play important roles. [0003] 5'-Nucleotidase is widely distributed in the human body, such as liver, gallbladder, pancreas, intestine, heart, brain, lung, kidney, pituitary, thyroid, prostate, testis and other organs and t...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
