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Culture medium capable of efficiently screening xanthine oxidase producing bacteria

A technology of xanthine oxidase and culture medium, which is applied in the field of culture medium for efficiently screening xanthine oxidase-producing bacteria

Active Publication Date: 2013-04-03
BEIJING LEADMAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has not been reported in the literature

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  • Culture medium capable of efficiently screening xanthine oxidase producing bacteria
  • Culture medium capable of efficiently screening xanthine oxidase producing bacteria
  • Culture medium capable of efficiently screening xanthine oxidase producing bacteria

Examples

Experimental program
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Effect test

example 1

[0039] 10 grams of peptone, 5 grams of yeast powder, 10 grams of sodium chloride, 1000 ml of water, adjust the pH to 7.00 ± 0.05 with 0.1 mol / liter hydrochloric acid or sodium hydroxide solution, add 0.2 grams of hypoxanthine, add 20 grams of agar, Sterilize at 120 degrees for 15 minutes. When the temperature drops below 45 degrees, add phenol (solution) to a final concentration of 0.1 g / L, mix well, and pour it into a flat plate (about 45 mm in diameter). The number of repetitions is 3.

[0040] After the plate is solidified, take 0.1 ml of the sample (soil dilution) and spread it on the plate, cultivate it at 30 degrees for 4 days, and see that colonies grow on the plate, pick the colonies and induce and detect the enzyme activity on the double screening medium. The number of colonies and the positive rate were counted, and the results are shown in Table 1.

example 2

[0042] 10 grams of peptone, 5 grams of yeast powder, 10 grams of sodium chloride, 1000 ml of water, adjust the pH to 7.00 ± 0.05 with 0.1 mol / L hydrochloric acid or sodium hydroxide solution, add 2 grams of hypoxanthine, add 20 grams of agar, Sterilize at 120 degrees for 15 minutes. When the temperature drops below 45 degrees, add phenol (solution) to a final concentration of 1 g / L, mix well, and pour it into a flat plate (about 45 mm in diameter). The number of repetitions is 3.

[0043] After the plate is solidified, take 0.1 ml of the sample (soil dilution) and spread it on the plate, cultivate it at 30 degrees for 4 days, and see that colonies grow on the plate, pick the colonies and induce and detect the enzyme activity on the double screening medium. The number of colonies and the positive rate were counted, and the results are shown in Table 1.

example 3

[0045] 10 grams of peptone, 5 grams of yeast powder, 10 grams of sodium chloride, 1000 ml of water, adjust the pH to 7.00 ± 0.05 with 0.1 mol / L hydrochloric acid or sodium hydroxide solution, add 5 grams of hypoxanthine, add 20 grams of agar, Sterilize at 120 degrees for 15 minutes. When the temperature drops below 45 degrees, add phenol (solution) to a final concentration of 2 g / L, mix well, and pour it into a flat plate (about 45 mm in diameter). The number of repetitions is 3.

[0046] After the plate is solidified, take 0.1 ml of the sample (soil dilution) and spread it on the plate, cultivate it at 30 degrees for 4 days, and see that colonies grow on the plate, pick the colonies and induce and detect the enzyme activity on the double screening medium. The number of colonies and the positive rate were counted, and the results are shown in Table 1.

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Abstract

The invention relates to a biological bacteria culture medium, in particular to a culture medium capable of efficiently screening xanthine oxidase producing bacteria. According to the culture medium, hypoxanthine and phenol are added into a common LB agar culture medium, so that growth of bacterial strains which do not generate xanthine oxidase can be inhibited, and bacterial strains which generate xanthine oxidase can selectively grow, as a result, the screening efficiency for biological bacteria is improved.

Description

technical field [0001] The invention relates to a biological bacteria culture medium, in particular to a culture medium capable of efficiently screening xanthine oxidase-producing bacteria. Background technique [0002] Xanthine oxidase is an important enzyme used in biochemical diagnostic reagents. It is often used to test adenosine deaminase and 5-nucleotidase in serum. Its stability, specificity, optimum pH, Km value, etc. Quality has an important impact. Chinese patent CN101402922 (an Arthrobacter and its application and preparation method for producing xanthine oxidase) discloses a method of using Arthrobacter to prepare xanthine oxidase, in order to further obtain an enzyme with better performance suitable for diagnosis It is necessary to screen new enzyme source strains and enzymes from soil. However, soil microorganisms are diverse. On ordinary screening plates, enzyme-producing bacteria and non-enzyme-producing bacteria grow in large numbers, and the screening wor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 不公告发明人
Owner BEIJING LEADMAN BIOCHEM
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