The invention belongs to the field of chemical synthesis and discloses a synthesis method for 4-S-5-Br-2',3',5'-O-triacetyluridine. The synthesis method comprises the steps that a compound 5-Br-2',3',5'-O-triacetyluridine serves as a raw material, phosphorus pentasulfide and a Lawesson reagent are combined to serve as a vulcanization reagent, 1,4-dioxane serves as a solvent, and a chemical reaction is carried out to finally prepare the 4-S-5-Br-2',3',5'-O-triacetyluridine. The synthesis method for the 4-S-5-Br-2',3',5'-O-triacetyluridine has the advantages that the reaction time is shortened,and the vulcanization efficiency is greatly improved. The synthesis method reduces the cost, the treatment after the reaction is simple and convenient, and meanwhile the yield of the thionucleoside compound after the reaction is increased.