96 results about "Adenosine Phosphates" patented technology

The invention discloses a 3 '-phosphoadenosine-5'-phosphoryl sulfate synthetase mutant and an application of the 3 '-phosphoadenosine-5'-phosphoryl sulfate synthetase mutant. Compared with a wild enzyme amino acid sequence as shown in SEQ ID NO.1, the amino acid sequence of the 3 '-adenosine-5'-phosphoryl sulfate synthetase mutant has one or two mutations in the 101 site, the 207 site and the 560 site; preferably, phenylalanine at the 101 site is mutated into tryptophan; cysteine at the 207th site is mutated into glycine; phenylalanine at the 560th site is mutated into tryptophan. The invention also provides a synthesis method and application of the 3 '-adenosine-5'-phosphoryl sulfuric acid. The APS intermediate product inhibition effect of hPAPSS1 is relieved through a point mutation technology, the substrate affinity of hPAPSS1 to ATP is improved, and a mutant enzyme with a high PAPS conversion rate is obtained; and the bifunctional enzyme hPAPSS1 is synthesized by heterologous expression of PAPS in escherichia coli, so that the acquisition of the enzyme is simplified, and the synthesis efficiency of PAPS is improved.

The present invention relates to a recombinant yeast cell that produces UDP-D-glucuronic acid and / or UDP-N-acetyl-D-glucosamine compared to a wild type cell of the recombinant yeast cell; and optionally, the yield of 3 '-phosphate-adenylate sulfuric acid is increased. The invention also relates to a method for producing heparan sulfate, which comprises a step of culturing the recombinant yeast cell, further relates to an application of the non-invasive recombinant yeast cell for producing heparan sulfate, and finally relates to heparan sulfate obtained by applying the method.

The invention discloses a novel biosynthesis path for producing 3 '-phosphoadenosine-5'-phosphosulfuric acid and application, and belongs to the technical field of bioengineering. According to the invention, through recombinant expression and purification of ribose kinase, ribose phosphate pyrophosphate kinase, ribose phosphate transferase, adenine thiotransferase, adenosine phosphate sulfate kinase and polyphosphate kinase 2, a six-enzyme cascade reaction taking ribose and adenosine as substrates in vitro is constructed, the molar conversion rate reaches 22.85%, and the escherichia coli and bacillus subtilis PAPS cell factory can be used for preparing the recombinant ribose phosphate pyrophosphate kinase, the ribose phosphate transferase, the adenine thiotransferase, the adenosine phosphate sulfate kinase and the polyphosphate kinase 2. The yield of PAPS in a 5-L fermentation tank reaches 7.60 g / L and 5.03 g / L respectively. The PAPS synthesis route provided by the invention provides an extensible, economical and efficient solution for the production of sulfated compounds.

The invention provides a high-yield L-cysteine engineering bacterium accumulated by sulfur donor 3 '-phosphoadenosine-5'-phosphoryl sulfuric acid as well as a construction method and application of the high-yield L-cysteine engineering bacterium. According to the method, the copy number of key genes in a cysteine synthesis route in an escherichia coli organism is changed, heterologous genes are introduced, the expression level of the key genes in the cysteine synthesis route in the escherichia coli organism is enhanced, and sulfur flux is further made to flow to synthesis of L-cysteine. On the basis that the chassis bacteria enhance the carbon flux, in order to maintain the balance of carbon and sulfur in the strain and continuously enhance the synthesis of the sulfur flux flowing to the L-cysteine, the synthesis of the product is promoted by enhancing the synthesis capability of a sulfur donor PAPS in a sulfate pathway, and finally, the plasmid-free and antibiotic-free engineering strain for high yield of the L-cysteine is obtained. Finally, the shake flask titer of the L-cysteine reaches 6.06 g / L and is increased by 49.2% compared with that of an original strain.

The invention belongs to the technical field of flame-retardant materials and composite boards, and particularly relates to a halloysite-based intumescent flame-retardant early-warning impregnation liquid as well as a preparation method and application thereof. A halloysite-based intumescent flame retardant prepared from melamine, adenosine triphosphate and halloysite nanotubes and a flame-retardant early-warning steeping liquor obtained by mixing graphene oxide are introduced into delignified wood, and then the flame-retardant densified wood integrating flame retardance, early warning and high mechanical strength is prepared. The flame-retardant wood has the characteristics of efficient flame retardance, intelligent fire early warning, excellent mechanical property and the like, widens the application range of wood and products thereof in the fields of buildings, home furnishing and the like, and has important significance for protecting life and property safety of people.

The invention relates to the technical field of enzyme catalysis, in particular to a 5 '-phosphosulfate kinase mutant and application thereof, and is characterized in that the 5'-phosphosulfate kinase mutant is obtained by performing single mutation or multiple mutation on an amino acid sequence as shown in SEQ ID NO: 1 and comprises one of the following mutation modes: (1) performing single mutation on the 66th site or 148th site of the amino acid sequence as shown in SEQ ID NO: 1; and (2) mutating the 66th site and 148th site of the amino acid sequence as shown in SEQ ID NO: 1 at the same time. Based on the design of the three-dimensional structure of the 5 '-phosphosulfate kinase, the 5'-phosphosulfate kinase mutant which can catalyze AMP to generate PAP and has obviously enhanced activity is obtained, the PAP activity of the 5 '-phosphosulfate kinase mutant with the highest activity is about 16 times that of a wild enzyme, and a solid foundation is laid for industrial production of 3'-adenosine-5 '-phosphosulfate.

The invention relates to the field of enzyme engineering, in particular to an enzyme mutant, an enzyme composition or an immobilized enzyme thereof, and application and a method for preparing L-L-carnitine by using the enzyme mutant and the enzyme composition or the immobilized enzyme thereof. The invention provides a simple process for preparing L-carnitine by a whole enzyme method, which is characterized in that bulk betaine (N, N, N-trimethylglycine) and acetic acid are used as raw materials in the whole process. L-carnitine is directly obtained through two-step continuous conversion of carboxyl reductase (CAR) and aldolase (DERA). The route can be completed in one step by utilizing crude enzyme liquid, recyclable immobilized enzyme can also be utilized, and reduced coenzyme II (NADPH) and adenosine triphosphate (ATP) which are used in the first step of reaction can also be recycled by utilizing corresponding enzyme, so that the production cost is further reduced. Therefore, the process for preparing the L-carnitine has the advantages of being completely green, simple in process, low in production cost, easy for large-scale production and the like.

The invention provides a yeast rich in polyphosphate and application of the yeast in synthesis of adenosine triphosphate, and the content of polyphosphate is 15-35 wt% based on the dry matter mass of the yeast rich in polyphosphate; according to the method disclosed by the invention, the yeast is subjected to phosphorus starvation culture and then is subjected to phosphorus supplementary culture, so that the yeast is enriched with polyphosphates, and the yeast rich in polyphosphates is obtained; then, the yeast rich in polyphosphate is used as a biocatalyst to synthesize ATP, that is, self-related enzyme systems, mainly including adenosine kinase, endo-polyphosphate kinase and excision-polyphosphate kinase, in the yeast rich in polyphosphate are used for replacing exogenous polyphosphate kinase and adenosine kinase, and in addition, ATP is synthesized by using the yeast rich in polyphosphate as a biocatalyst. According to the method for synthesizing ATP by using the yeast rich in polyphosphate as the biocatalyst, exogenous adenosine kinase and exogenous polyphosphate kinase do not need to be additionally added, the cost is low, the efficiency of converting adenosine into ATP is high, the batch-to-batch stability of the yeast is realized, and the enzyme activity of the self-related enzyme system is high.

The invention discloses a method for catalytically synthesizing adenosine triphosphate by using microcrystalline cellulose carrier purification immobilized enzyme, which comprises the following steps: preparing bacterial suspension by using escherichia coli wet thalli and Tris-HCL buffer solution, crushing, centrifuging, and taking supernate to obtain crude enzyme; adding a microcrystalline cellulose carrier and the wet thalli into the crude enzyme liquid, and washing the immobilized enzyme after immobilization to obtain the immobilized enzyme; the method comprises the following steps: adding immobilized enzyme adenosine kinase and immobilized polyphosphate kinase into a substrate system with the volume of 1L according to a weight ratio, and starting a catalytic reaction under the conditions that the pH is 5.5-7.5 and the temperature is 20-40 DEG C; after catalysis is finished, the immobilized enzyme adenosine kinase and the immobilized polyphosphate kinase are subjected to solid-liquid separation with reaction liquid through a Buchner funnel, the immobilized enzyme continues to be subjected to catalytic reaction, and the method for preparing the immobilized enzyme is simple in mode, low in cost, easy to industrially produce, high in stability and capable of being repeatedly used for many times, and products are easy to separate.

The invention relates to an adenosine triphosphate optical probe, in particular to an adenosine triphosphate (ATP) optical probe as well as a preparation method and application thereof. Specifically, the invention provides an adenosine triphosphate optical probe, which comprises an adenosine triphosphate sensitive polypeptide and an optically active polypeptide, and the optically active polypeptide is located in the sequence of the adenosine triphosphate sensitive polypeptide.

The present disclosure is directed to inhibitors of the CBP / p300 family of bromodomains. The compounds can be useful in the treatment of disease or disorders associated with the inhibition of the CBP / p300 family of bromodomains. For instance, the disclosure is concerned with compounds and compositions for inhibition of the CBP / p300 family of bromodomains, methods of treating diseases or disorders associated with the inhibition of CBP / p300 family of bromodomains (e.g., certain forms of cancer), and methods of synthesis of these compounds.

The present invention relates to the field of enzyme engineering, and more particularly to enzyme mutants, enzyme compositions or their immobilized enzymes and their applications and methods for preparing L-carnitine. The present invention provides a simple L-carnitine holoenzyme preparation process, the overall process of which utilizes bulk betaine (N,N,N-trimethylglycine) and acetic acid as raw materials. L-carnitine is directly obtained by two-step continuous conversion by carboxyl reductase (CAR) and aldolase (DERA). This route can be completed in one step using a crude enzyme solution, or by utilizing a recyclable immobilized enzyme, and the reduced coenzyme II (NADPH) and adenosine triphosphate (ATP) used in the first step of the reaction can also be recycled using corresponding enzymes to further reduce production costs. Therefore, the patented L-carnitine preparation process has many advantages, such as being fully green, simple in process, inexpensive to produce, and easy to scale up.

The invention discloses a CK-MB-containing biochemical multi-term liquid quality control product and a preparation method thereof, and belongs to the technical field of biochemical quality control products, the added substances comprise an adenosine phosphate compound, a creatine compound and an auxiliary stabilizer, the adenosine phosphate compound is at least one of adenosine diphosphate and adenosine triphosphate, and the creatine compound is at least one of creatine triphosphate and creatine triphosphate. The creatine compound is at least one of creatine and phosphocreatine, and the auxiliary stabilizer is at least one of acetylcysteine and beta-mercaptoethanol; and the CK-MB-containing biochemical multi-liquid quality control product is a frozen liquid. The adenylate phosphate compound, the creatine compound and the auxiliary stabilizer are added into the liquid quality control product, so that multiple protection mechanisms can be realized, and the stability of multiple biochemical liquid quality control products containing CK-MB, especially the stability of CK-MB, is obviously improved; errors caused by redissolution are avoided, the detection convenience and accuracy of inspection personnel are greatly improved, and the labor and time cost of a laboratory is reduced.

The invention relates to the technical field of biosynthesis, in particular to a biosynthesis process of adenosine triphosphate and equipment thereof. Comprising the following steps: optimizing an adenosine triphosphate synthetase gene sequence, constructing an expression vector, and introducing the expression vector into a yeast host cell; sub-packaging the engineering yeast group in a pore plate for parallel culture, detecting the acid yield and substrate conversion rate data of each micropore, and screening out elite strains; fermenting elite strains, and dynamically feeding materials by an online monitoring and feedback control system; the yeast strain is subjected to precise transformation through codon optimization and gene overexpression driven by a strong promoter; elite strains are quickly identified by adopting high-throughput screening in cooperation with miniaturized parallel fermentation and real-time optical detection; in the fermentation process, a self-control feedback fed-batch technology is introduced, and a culture environment is stabilized in an optimal physiological state through real-time monitoring and dynamic regulation, so that the production potential of engineering bacteria is fully exerted, and the yield of adenosine triphosphate, the substrate conversion rate and the product purity are improved.

The application belongs to the technical field of molecular biology, and particularly relates to metabolic markers for distinguishing hypertrophic cardiomyopathy and left ventricular hypertrophy and application. The application analyzes the metabolome of hypertrophic cardiomyopathy (HCM) patients, left ventricular hypertrophy (LVH) patients caused by non-hypertrophic cardiomyopathy and healthy people, finds that carnitine C16:2, carnitine C14:0, carnitine C16:0, carnitine C18:0 and carnitine C18:1 are differentially expressed in HCM patients and LVH patients, pyridoxine phosphate, ito acid, adenosine monophosphate, adenosine diphosphate and adenosine triphosphate are differentially expressed in HCM patients and healthy people, lysine base, methyl glutamic acid, acetyl proline, malate semialdehyde and citramalate are differentially expressed in LVH patients and healthy people, and ROC curve analysis shows excellent prediction effect, strong specificity and high sensitivity.

The invention provides a beta-galactosidase compound and a method. The beta-galactosidase compound comprises beta-galactosidase and cyclic adenosine monophosphate combined with amino acid residues of the beta-galactosidase. According to the invention, small molecular cyclic adenosine monophosphate acts on lactase molecules, and the catalytic activity of lactase is significantly enhanced through unique intermolecular interaction. Compared with a traditional lifting means, the method has the advantages that the lifting efficiency is higher, an ideal lactase activity level can be achieved in a shorter time, and a powerful guarantee is provided for lactose digestion and utilization.

The present invention relates to a method and system for producing adenosine triphosphate (ATP) through an acid / aldehyde-ATP (AAA) cycle in an electrochemical cell.

The invention provides a method for simultaneously determining various flavor nucleotides in a livestock and poultry meat product and application, and the method comprises the following steps: freeze-drying the livestock and poultry meat product, and then preparing the freeze-dried livestock and poultry meat product into a test solution; measuring the contents of CMP (cytidylic acid), ATP (adenosine triphosphate), GMP (guanine monophosphate), IMP (inosinic acid), ADP (adenosine diphosphate), Hy (hypoxanthine), AMP (adenosine monophosphate) and IN (inosine) in the test solution by adopting HPLC (high performance liquid chromatography); in HPLC, a mobile phase A is a phosphate buffer solution with the volume concentration of 95%, a mobile phase B is methanol with the volume concentration of 5%, gradient elution is carried out, and the gradient elution condition is 0-11 min, 99% phosphate buffer solution and 1% methanol; when 12 min, 80% phosphate buffer solution and 20% methanol are added, and the conditions are maintained for 6-7 min; the phosphate buffer solution is heated to 99% within 2 minutes, and then the temperature is kept for 8-10 minutes.

This disclosure relates to the use of glycogen phosphatase B agonists in the treatment of neurodegenerative diseases. Specifically, this disclosure provides the use of glycogen phosphatase B agonists (preferably adenosine monophosphate) in the preparation of medicaments for the prevention or treatment of neurodegenerative diseases. This disclosure demonstrates that glycogen phosphatase B agonists (AMPs) hold promise as a safe and effective treatment option for patients with neurodegenerative diseases.

The invention belongs to the field of insecticidal and acaricidal agents, and relates to an insecticidal and acaricidal composition. The composition contains an active component A and an active component B, and the weight part ratio of the active component A to the active component B is (1: 99)-(99: 1); the active component A is selected from a compound I; the active component B is selected from inhibitors influencing the growth of chitin synthetase 1 (CHS1) mites, mitochondrial adenosine triphosphate (ATP) synthetase inhibitors, mitochondrial electron transfer complex (III) inhibitors, mitochondrial electron transfer complex (I) inhibitors, acetyl-CoA carboxylase inhibitors and mitochondrial electron transfer complex (II) inhibitors; a trifluoroethyl sulfide type acaricide or other types of insecticidal and acaricide; the composition disclosed by the invention has the advantages of obvious synergism, resistance delay and the like, and can be used for preventing and treating various pests.

The invention relates to non-naturally occurring mutated arylsulfotransferases comprising (i) an amino acid substitution in at least one amino acid position selected among positions 6, 7, 8, 9, 11, 17, 20, 33, 62, 97, 138, 195, 236, 239, 244, 263, and combinations thereof, wherein the position is relative to the amino acids sequence of rat arylsulfotransferase IV SEQ ID NO: 1 and (ii) an amino acid sequence having at least 60% sequence identity with amino acids sequence SEQ ID NO: 1. The mutated arylsulfotransferase may have a sulfotransferase activity for converting adenosine 3′,5′-bisphosphate (PAP) into 3′-phosphoadenosine-5′-phosphosulfate (PAPS) enhanced compared to the wild-type enzyme.

The present invention belongs to the technical field of enzyme genetic engineering, and specifically relates to a method for synthesizing and preparing a glycerol derivative. The present application co-expresses polyphosphate kinase and glycerol kinase, thereby improving the ability of polyphosphate kinase to regenerate ATP. The co-expressed polyphosphate kinase and glycerol kinase are immobilized, and the immobilized enzyme is applied to the batch preparation of GPA, using glycerol and ADP as substrates, and using inexpensive PolyP to provide phosphate groups, thereby solving the problem of expensive ATP and realizing low-cost preparation of GPA. The co-expressed polyphosphate kinase glycerol kinase is synthetically coupled with GPCMP, and inexpensive PolyP is used to provide phosphate groups, using glycerol, adenosine diphosphate (ADP), and cytidine monophosphate (CMP) as substrates, thereby solving the problem of expensive ATP and cytidine triphosphate (CTP), thereby realizing low-cost preparation of GPCMP.

The invention relates to a traditional Chinese medicine composition for treating ischemic heart failure and a preparation method thereof, and belongs to the field of traditional Chinese medicine pharmacy, and the traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 5-15 parts of cassia twig, 5-15 parts of bighead atractylodes rhizome, 5-15 parts of poria cocos, 6-12 parts of rhizoma zingiberis, 5-15 parts of acanthopanax, 10-30 parts of fructus polygoni orientalis, 3-9 parts of verbena, 6-12 parts of dogwood and 3-9 parts of honey-fried licorice root. The composition can remarkably reduce the level of N-terminal proB-type natriuretic peptide, remarkably improve pathological changes of heart tissues of model rats, regulate and control the heart functions of the model rats, improve the systolic function of the heart, relieve expression of inflammatory factors such as interleukin-1beta, interleukin-6 and tumor necrosis factor-alpha of the rats and improve the anti-inflammatory effect of the rats. The action mechanism of the compound relates to a plurality of pathways such as extracellular matrix receptor interaction, a chemotactic factor signal pathway, a relaxin signal pathway, a cyclic adenosine monophosphate signal pathway, a nuclear factor-kB signal pathway, fatty acid metabolism and a Toll-like receptor signal pathway, and the compound is an effective medicament for treating ischemic heart failure.

The present invention belongs to the technical field of preparing functional materials for molecular recognition, adsorption and separation, and discloses a method for preparing a hydrophilic dual-receptor MOFs nanosheet surface imprinted adsorbent and its application in the selective separation of adenosine monophosphate. The present invention targets the structural characteristics of a typical nucleoside compound adenosine monophosphate (AMP) molecule, designs and synthesizes Zr-MOFs nanosheets coordinated with its specific metal ion as a matrix material, and uses 5-(2-methoxyvinyl)-2′-deoxyuridine (AcrU) as a functional monomer that undergoes specific base complementary pairing. The arrangement and orientation of the template molecules are precisely fixed through an ordered assembly strategy. Finally, the large number of double bonds modified on the surface of the Zr-MOFs nanosheets and a hydrophilic cross-linking agent are utilized to photoinitiate the construction of a stable hydrophilic dual-receptor MOFs nanosheet surface imprinted adsorbent (D-MIPs), thereby enhancing the recognition and separation effect of nucleoside compounds and achieving the selective separation and enrichment of nucleoside compounds such as AMP in biological samples such as urine and blood.

The present invention relates to a method of diagnosing over active bladder disorder (OAB), the method comprising: measuring the concentrations of adenosine triphosphate (ATP), acetylcholine (ACh), nitrite, monocyte chemoattractant protein 1 (MCP-1) and interleukin 5 (IL-5) in a sample obtained from a subject; normalising the concentrations to the concentration of creatinine (Cr) in the sample; range standardising the normalised concentrations and subject's age to the following values: Age to 120 years old; ATP / Cr to 0.000001 mole / mg×dl−1; ACh / Cr to 0.1 mole / mg×dl−1; Nitrite to 200 nM / mg×dl−1; MCP-1 / Cr to 100 pg×ml−1 / mg×dl−1; IL-5 / Cr 100 pg×ml-mg×dl−1; applying the normalised and range standardised concentrations to the following formula: Logit (p)=−1.738±1.404±4.985±2.914×subject's age+3315.959±5435.254×[ATP] / [Cr]+(−25204.194±20268.337)×[ACh] / [Cr]+26.799±32.967×[nitrite] / [Cr]+6.755±25.132 [MCP-1] / [Cr]+(−61.838±148.740) [IL-5] / [Cr] and calculating Logit; wherein a Logit value above a predetermined threshold indicates that the subject has OAB. Methods for monitoring the progression of OAB using the method, kits for use in the method and computer systems and programs configured to execute the method are also provided.

The present invention relates to the field of biosynthesis technology, and more particularly to a β-nicotinamide mononucleotide production system and a method for preparing β-nicotinamide mononucleotide in a dual-enzyme collaborative manner. The β-nicotinamide mononucleotide production system comprises an NRK-HS01 whole-cell catalyst, an ADK whole-cell catalyst, nicotinamide riboside, adenosine triphosphate, and MgCl2. The present invention couples the NRK-HS01 whole-cell catalyst with an ATP regeneration system (ADK whole-cell catalyst) to catalyze the conversion of nicotinamide riboside and adenosine triphosphate to produce NMN in a single step. This method achieves an NMN molar yield exceeding 99%, reduces ATP costs by 50%, and reduces feedback inhibition of the synthesis reaction caused by the accumulation of the byproduct ADP. This method also enables the recycling of ATP, thereby improving synthesis efficiency and saving production costs. This method is of great significance for the industrial development of biosynthesis of NMN.

The present invention relates to a blood treatment device configured to dephosphorylate extracellular adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and / or lipopolysaccharide (LPS) in the blood of a patient in need thereof in an extracorporeal blood circuit, wherein the device comprises a matrix on which alkaline phosphatase (AP) is immobilized. The present invention also relates to an extracorporeal blood circuit comprising a blood treatment device of the present invention and to the use of the blood treatment device as a medicament or a method for treating an infection, preferably a blood or systemic infection such as sepsis, and / or for treating sepsis-related acute kidney injury (AKI).

To provide an O / W type high-viscosity emulsion composition containing adenosine phosphate and / or a salt thereof, and acrylic acid / alkyl methacrylate copolymer.SOLUTION: The present disclosure provides an O / W type emulsion composition that contains adenosine phosphate and / or a salt thereof, an acrylic acid / alkyl methacrylate copolymer, oil, and water, and that contains a pH adjuster that is a water-soluble polymer and / or an organic alkali selected from a swelling type thickener, an associative type thickener, a polysaccharide and a derivative thereof, and a mixture thereof, and that has a viscosity of 10,000 mPa s or more.SELECTED DRAWING: Figure 1

The invention discloses a recombinant plant lactobacillus for overexpressing an adenosine triphosphate binding cassette transporter protein gene and application of the recombinant plant lactobacillus. Belongs to the technical field of bioengineering. The invention aims to improve the cold-resistant growth capability of the plant lactobacillus, increase the capability of producing lactic acid and degrading nitrite, and improve the integrity and permeability of the plant lactobacillus cells. The invention provides a recombinant lactobacillus plantarum, which is characterized in that the lactobacillus plantarum is used as an original strain, and a nucleic acid molecule as shown in SEQ ID NO.1 is overexpressed. The method is used for fermenting products in food industry.

The application discloses a salt-tolerant adenylate kinase mutant and application thereof. Through modification on an amino acid sequence of a wild-type adenylate kinase (adenylate kinase, EC 2.7.4.3) from Bacillus subtilis (strain 168), three salt-tolerant adenylate kinase mutants are obtained; the mutant adenylate kinase has higher salt tolerance compared with the wild-type adenylate kinase, and can tolerate a salt concentration of 300-2000 mmol / L in an adenosine diphosphate reaction system.