53 results about "Adenosine Phosphates" patented technology

The invention provides a yeast rich in polyphosphate and application of the yeast in synthesis of adenosine triphosphate, and the content of polyphosphate is 15-35 wt% based on the dry matter mass of the yeast rich in polyphosphate; according to the method disclosed by the invention, the yeast is subjected to phosphorus starvation culture and then is subjected to phosphorus supplementary culture, so that the yeast is enriched with polyphosphates, and the yeast rich in polyphosphates is obtained; then, the yeast rich in polyphosphate is used as a biocatalyst to synthesize ATP, that is, self-related enzyme systems, mainly including adenosine kinase, endo-polyphosphate kinase and excision-polyphosphate kinase, in the yeast rich in polyphosphate are used for replacing exogenous polyphosphate kinase and adenosine kinase, and in addition, ATP is synthesized by using the yeast rich in polyphosphate as a biocatalyst. According to the method for synthesizing ATP by using the yeast rich in polyphosphate as the biocatalyst, exogenous adenosine kinase and exogenous polyphosphate kinase do not need to be additionally added, the cost is low, the efficiency of converting adenosine into ATP is high, the batch-to-batch stability of the yeast is realized, and the enzyme activity of the self-related enzyme system is high.

The invention discloses a method for catalytically synthesizing adenosine triphosphate by using microcrystalline cellulose carrier purification immobilized enzyme, which comprises the following steps: preparing bacterial suspension by using escherichia coli wet thalli and Tris-HCL buffer solution, crushing, centrifuging, and taking supernate to obtain crude enzyme; adding a microcrystalline cellulose carrier and the wet thalli into the crude enzyme liquid, and washing the immobilized enzyme after immobilization to obtain the immobilized enzyme; the method comprises the following steps: adding immobilized enzyme adenosine kinase and immobilized polyphosphate kinase into a substrate system with the volume of 1L according to a weight ratio, and starting a catalytic reaction under the conditions that the pH is 5.5-7.5 and the temperature is 20-40 DEG C; after catalysis is finished, the immobilized enzyme adenosine kinase and the immobilized polyphosphate kinase are subjected to solid-liquid separation with reaction liquid through a Buchner funnel, the immobilized enzyme continues to be subjected to catalytic reaction, and the method for preparing the immobilized enzyme is simple in mode, low in cost, easy to industrially produce, high in stability and capable of being repeatedly used for many times, and products are easy to separate.

The invention relates to an adenosine triphosphate optical probe, in particular to an adenosine triphosphate (ATP) optical probe as well as a preparation method and application thereof. Specifically, the invention provides an adenosine triphosphate optical probe, which comprises an adenosine triphosphate sensitive polypeptide and an optically active polypeptide, and the optically active polypeptide is located in the sequence of the adenosine triphosphate sensitive polypeptide.

The invention relates to the technical field of biosynthesis, in particular to a biosynthesis process of adenosine triphosphate and equipment thereof. Comprising the following steps: optimizing an adenosine triphosphate synthetase gene sequence, constructing an expression vector, and introducing the expression vector into a yeast host cell; sub-packaging the engineering yeast group in a pore plate for parallel culture, detecting the acid yield and substrate conversion rate data of each micropore, and screening out elite strains; fermenting elite strains, and dynamically feeding materials by an online monitoring and feedback control system; the yeast strain is subjected to precise transformation through codon optimization and gene overexpression driven by a strong promoter; elite strains are quickly identified by adopting high-throughput screening in cooperation with miniaturized parallel fermentation and real-time optical detection; in the fermentation process, a self-control feedback fed-batch technology is introduced, and a culture environment is stabilized in an optimal physiological state through real-time monitoring and dynamic regulation, so that the production potential of engineering bacteria is fully exerted, and the yield of adenosine triphosphate, the substrate conversion rate and the product purity are improved.

The application belongs to the technical field of molecular biology, and particularly relates to metabolic markers for distinguishing hypertrophic cardiomyopathy and left ventricular hypertrophy and application. The application analyzes the metabolome of hypertrophic cardiomyopathy (HCM) patients, left ventricular hypertrophy (LVH) patients caused by non-hypertrophic cardiomyopathy and healthy people, finds that carnitine C16:2, carnitine C14:0, carnitine C16:0, carnitine C18:0 and carnitine C18:1 are differentially expressed in HCM patients and LVH patients, pyridoxine phosphate, ito acid, adenosine monophosphate, adenosine diphosphate and adenosine triphosphate are differentially expressed in HCM patients and healthy people, lysine base, methyl glutamic acid, acetyl proline, malate semialdehyde and citramalate are differentially expressed in LVH patients and healthy people, and ROC curve analysis shows excellent prediction effect, strong specificity and high sensitivity.

The invention provides a beta-galactosidase compound and a method. The beta-galactosidase compound comprises beta-galactosidase and cyclic adenosine monophosphate combined with amino acid residues of the beta-galactosidase. According to the invention, small molecular cyclic adenosine monophosphate acts on lactase molecules, and the catalytic activity of lactase is significantly enhanced through unique intermolecular interaction. Compared with a traditional lifting means, the method has the advantages that the lifting efficiency is higher, an ideal lactase activity level can be achieved in a shorter time, and a powerful guarantee is provided for lactose digestion and utilization.

This disclosure relates to the use of glycogen phosphatase B agonists in the treatment of neurodegenerative diseases. Specifically, this disclosure provides the use of glycogen phosphatase B agonists (preferably adenosine monophosphate) in the preparation of medicaments for the prevention or treatment of neurodegenerative diseases. This disclosure demonstrates that glycogen phosphatase B agonists (AMPs) hold promise as a safe and effective treatment option for patients with neurodegenerative diseases.

The invention belongs to the field of insecticidal and acaricidal agents, and relates to an insecticidal and acaricidal composition. The composition contains an active component A and an active component B, and the weight part ratio of the active component A to the active component B is (1: 99)-(99: 1); the active component A is selected from a compound I; the active component B is selected from inhibitors influencing the growth of chitin synthetase 1 (CHS1) mites, mitochondrial adenosine triphosphate (ATP) synthetase inhibitors, mitochondrial electron transfer complex (III) inhibitors, mitochondrial electron transfer complex (I) inhibitors, acetyl-CoA carboxylase inhibitors and mitochondrial electron transfer complex (II) inhibitors; a trifluoroethyl sulfide type acaricide or other types of insecticidal and acaricide; the composition disclosed by the invention has the advantages of obvious synergism, resistance delay and the like, and can be used for preventing and treating various pests.

The invention relates to a traditional Chinese medicine composition for treating ischemic heart failure and a preparation method thereof, and belongs to the field of traditional Chinese medicine pharmacy, and the traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 5-15 parts of cassia twig, 5-15 parts of bighead atractylodes rhizome, 5-15 parts of poria cocos, 6-12 parts of rhizoma zingiberis, 5-15 parts of acanthopanax, 10-30 parts of fructus polygoni orientalis, 3-9 parts of verbena, 6-12 parts of dogwood and 3-9 parts of honey-fried licorice root. The composition can remarkably reduce the level of N-terminal proB-type natriuretic peptide, remarkably improve pathological changes of heart tissues of model rats, regulate and control the heart functions of the model rats, improve the systolic function of the heart, relieve expression of inflammatory factors such as interleukin-1beta, interleukin-6 and tumor necrosis factor-alpha of the rats and improve the anti-inflammatory effect of the rats. The action mechanism of the compound relates to a plurality of pathways such as extracellular matrix receptor interaction, a chemotactic factor signal pathway, a relaxin signal pathway, a cyclic adenosine monophosphate signal pathway, a nuclear factor-kB signal pathway, fatty acid metabolism and a Toll-like receptor signal pathway, and the compound is an effective medicament for treating ischemic heart failure.

The application discloses a salt-tolerant adenylate kinase mutant and application thereof. Through modification on an amino acid sequence of a wild-type adenylate kinase (adenylate kinase, EC 2.7.4.3) from Bacillus subtilis (strain 168), three salt-tolerant adenylate kinase mutants are obtained; the mutant adenylate kinase has higher salt tolerance compared with the wild-type adenylate kinase, and can tolerate a salt concentration of 300-2000 mmol / L in an adenosine diphosphate reaction system.

The invention belongs to the technical field of biological detection and analysis, and particularly relates to an adenosine phosphate photoaffinity probe as well as a preparation method and application thereof. The probe comprises an AMP-N (adenosine monophosphate-N) photoaffinity probe, an ADP-N (adenosine monophosphate-N) photoaffinity probe and an ATP-N (adenosine monophosphate-N) photoaffinity probe, the phosphoric acid part of adenosine monophosphate is modified with a photoaffinity group diaziridine with a small volume and alkynyl capable of carrying out a biological orthogonal reaction, and a connecting bond capable of cracking under an acidic condition is designed between adenosine monophosphate and a photoreactive group. The probe is high in stability and good in marking efficiency, can specifically mark the adenosine phosphate binding protein and identify the binding site of the adenosine phosphate binding protein, is suitable for screening, functional research and affinity analysis of the adenosine phosphate binding protein and discovery and verification of related drug targets, and has a wide application prospect.

The application provides an adenosine triphosphate and nitric oxide dual-response near-infrared fluorescent probe and a preparation method and application thereof. The near-infrared fluorescent probe has the structure shown in the following formula I, is responsive to both adenosine triphosphate and nitric oxide, and has high sensitivity and selectivity.

The application discloses a cyclic GMP-AMP synthetase mutant and a method for preparing 2',3'-cyclic guanosine monophosphate-adenosine monophosphate by using the cyclic GMP-AMP synthetase mutant, wherein the amino acid sequence of the cyclic GMP-AMP synthetase mutant has any one of the following mutations: P361A, L377M, K432R, S221Y / F357L / I496V, S221L / Y248F / K432H / S434T, F357L / P361A / L377M / S434T, F357L / P361A / S434T / H437T or H437T / S434T, compared with a wild type as shown in SEQ ID NO. 1. The activity of the cGAS mutant of the application can reach 4 times of the wild type, raw materials of the preparation method are economical and easy to obtain, the operation is simple, green, environment-friendly, pollution-free, the synthesis efficiency is high, and industrial development is easy.

The invention belongs to the technical field of biosensing and underwater detection, and particularly relates to an underwater adenosine triphosphate detection system and method. According to the system, a nanogold modified screen-printed electrode is used as a sensing platform, based on an electrochemical aptamer sensing principle, an ATP specific nucleic acid aptamer is fixed in an Au-S bond self-assembly mode to form a molecular recognition interface, and when ATP is combined with the aptamer, characteristics such as interface electron transfer resistance are changed, so that the ATP specific nucleic acid aptamer is identified. The high-sensitivity and high-selectivity detection of ATP is realized by detecting the peak current change of the electroactive reporter molecule; meanwhile, an epoxy resin-silicone rubber-silane coupling agent composite packaging system is adopted, a compact protection layer is formed in a non-working area of the electrode through a stepped heating curing process, and the three parts cooperate to ensure the sealing performance and long-term stability in an underwater environment, so that underwater in-situ detection in a real sense is realized; the target object degradation in the sample collection and storage process is avoided. The method has important value in the fields of marine environment monitoring, underwater biological research and the like.

The invention belongs to the field of insecticides and acaricides, and relates to a composition containing alanine-isoxazoline compounds and acaricides. The composition contains an active component A and an active component B, wherein the weight part ratio of the active component A to the active component B is (1: 100)-(100: 1); the active component A is selected from a compound I, a compound II or a stereoisomer thereof; the active component B is selected from inhibitors influencing the growth of chitin synthetase 1 (CHS1) mites, mitochondrial adenosine triphosphate (ATP) synthetase inhibitors, mitochondrial electron transfer complex (III) inhibitors, mitochondrial electron transfer complex (I) inhibitors, acetyl-CoA carboxylase inhibitors and mitochondrial electron transfer complex (II) inhibitors; a trifluoroethyl sulfide type acaricide or other types of insecticidal and acaricide; the composition disclosed by the invention has the advantages of obvious synergism, resistance delaying and the like, and can be used for preventing and treating various pests.

The present invention addresses the problem of providing: a composition that increases the amount of intracellular ATP and the amount of intracellular NAD; and a composition that improves motor performance. More specifically, the present invention provides a composition for increasing the amounts of intracellular adenosine triphosphate (ATP) and intracellular nicotinamide adenine dinucleotide (NAD), the composition being obtained by combining (a) and (b): (a) an NAD precursor or a pharmaceutically acceptable salt thereof; and (b) one or more substances selected from the group consisting of uridine and uridine precursors, or one or more pharmaceutically acceptable salts thereof, and / or one or more substances selected from the group consisting of inosine and inosine precursors, or one or more pharmaceutically acceptable salts thereof.　Furthermore, the present invention provides a composition for enhancing muscle strength, the composition being characterized by inclluding a combination of (c) and (d): (c) one or more substances selected from the group consisting of β-alanine and β-alanine precursors, or one or more pharmaceutically acceptable salts thereof; and (d) one or more substances selected from the group consisting of uridine and uridine precursors, or one or more pharmaceutically acceptable salts thereof, and / or one or more substances selected from the group consisting of inosine and inosine precursors, or one or more pharmaceutically acceptable salts thereof.

The application discloses a method for preparing 2'-methoxy-uridine triphosphate, which comprises the following steps: taking 2'-methoxy-uridine acid as a substrate, taking adenosine triphosphate as an initial phosphate donor, constructing a phosphate circulation system, adding an enzyme catalyst, and adopting one-pot enzyme method to perform enzyme catalysis reaction, so as to obtain the end product 2'-methoxy-uridine triphosphate. The phosphate circulation system is composed of adenosine triphosphate, adenosine diphosphate and phosphate compounds. The enzyme catalyst catalyzes the dephosphorylation of the phosphate compounds to provide inorganic phosphate to the adenosine diphosphate, and the adenosine triphosphate is regenerated to realize the circulation energy supply. The method adopts high-activity enzyme combination of specific sources, has high conversion rate of the substrate 2'-methoxy-uridine acid, and has high conversion rate of the final 2'-methoxy-uridine triphosphate up to 92.6%, and has the advantages of high synthesis efficiency, simple steps, green environmental protection and controllable cost.

The invention discloses a method for visually detecting various phosphates, which comprises the following steps: firstly, synthesizing three MOF (Metal Organic Framework) nano-enzymes, namely PCN-222 (Fe), PCN-223 (Fe) and PCN-224 (Fe) with peroxidase-like activity, catalyzing a colorless substrate 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) into oxTMB in a blue oxidation state by the three MOF nano-enzymes in the presence of H2O2, and generating remarkable colorimetric response; then, the five phosphates, namely ATP (adenosine triphosphate), ADP (adenosine diphosphate), AMP (adenosine monophosphate), PPi (pyrophosphoric acid) and Pi (inorganic phosphate), are simultaneously distinguished and detected by utilizing the principle that different phosphates have different regulation degrees on the peroxidase-like activity of the MOF nano-enzyme. In combination with a smartphone application program 'ColorMax', real-time visual analysis of phosphate is realized, and an efficient and convenient method is provided for distinguishing and detecting different phosphates.

The invention relates to a property-changed glucose-1-adenosine monophosphate transferase mutant and application thereof, and belongs to the technical field of enzyme engineering. The glucose-1-phosphoadenylyltransferase mutant is subjected to mutation and deletion of different sites, the property of the obtained glucose-1-phosphoadenylyltransferase mutant is changed, particularly, the thermal stability of the glucose-1-phosphoadenylyltransferase mutant is improved, the residual enzyme activity of the glucose-1-phosphoadenylyltransferase mutant at 30 DEG C is remarkably improved, and the residual enzyme activity of the glucose-1-phosphoadenylyltransferase mutant at 65 DEG C is also greatly improved. Therefore, in the process of catalyzing glucose-1-phosphate to generate ADP-glucose, the glucose-1-phosphate adenylyl transferase mutant disclosed by the invention can tolerate a high-heat environment generated by thallus fermentation, and has wide application value in industrial application.

The present disclosure relates to uses and methods implementing a cell free extract (CFE) having an activity for converting adenosine 2',3'-cyclic phosphate 5'-phosphosulfate (cyclo-PAPS) into 3'-phosphoadenosine-5'-phosphosulfate (PAPS) useful for sulfating a substrate.

The present invention discloses a recombinant Lactobacillus plantarum that overexpresses an ATP-binding cassette transporter protein gene and its application. This relates to the field of bioengineering technology. The present invention aims to improve the cold-resistant growth ability of Lactobacillus plantarum, increase its ability to produce lactic acid and degrade nitrite, and improve the integrity and permeability of Lactobacillus plantarum cells. Lactiplantibacillus plantarum ), using Lactobacillus plantarum as the starting strain, overexpressing the nucleic acid molecule shown in SEQ ID NO. 1. It is used for fermentation products in the food industry.

The invention relates to a 3 '-phosphoadenosine-5'-phosphoryl sulfuric acid biosynthesis system based on ATP (adenosine triphosphate) regeneration and enzyme immobilization and application. The invention firstly provides an enzyme compound which comprises ATP (adenosine triphosphate) regeneration enzyme and PAPS (Phosphatidylcholine) synthetase; the ATP regeneration enzyme is immobilized PPK labeled by streptavidin-biotin; the PAPS synthetase is composed of immobilized ATPS labeled by streptavidin-biotin, APSK and PPA, and the immobilized ATPS labeled by streptavidin-biotin, the APSK and The mass ratio of the ATP regeneration enzyme to the PAPS synthetase is (1-1.5): 2. The invention provides a method for synthesizing 3 '-adenosine-5'-phosphoryl sulfuric acid by using an enzyme compound, so that high-efficiency and low-cost synthesis of PAPS is realized, and the problems of high cost and poor conversion efficiency of a traditional PAPS synthesis method are effectively solved. The invention further provides a 3 '-adenosine-5'-phosphoryl sulfuric acid biosynthesis system based on ATP regeneration and enzyme immobilization on the basis of the enzyme compound, and the system utilizes yeast cells as an ATP supply source to realize efficient conversion from adenosine to ATP.

This invention discloses a liver-protective composition containing burdock root aqueous extract, its preparation method, and its application. The composition comprises six traditional Chinese medicinal herbs: burdock root aqueous extract, jujube seed extract, lily extract, wolfberry extract, white chrysanthemum extract, and poria cocos extract; seven nutrients: vitamin D3, vitamin E, inositol, choline, pyrrolizidine quinoline quinone, magnesium glycine, and zinc citrate; and three flavoring agents: semaside, mogroside, and adenosine monophosphate (AMP). AMP has both umami enhancement and AMPK activation effects. The composition is prepared into powder or tablets using low-temperature freeze-drying or low-temperature spray drying. The composition of this invention has a significant protective effect against chemically induced liver damage and sleep deprivation-induced liver damage, and is suitable for liver protection and health maintenance in people who stay up late, overuse their eyes, experience high mental stress, and are middle-aged and elderly.

The invention discloses a method for preparing 2 '-methoxy-uridine triphosphate, which comprises the following steps: in a liquid reaction system, constructing a phosphoric acid circulating system by taking 2'-methoxy-uridine monophosphate as a substrate and adenosine triphosphate as an initial phosphoric acid donor, adding an enzyme catalyst, and carrying out an enzyme catalysis reaction by adopting a one-pot enzyme method to obtain the 2 '-methoxy-uridine triphosphate. The phosphoric acid circulating system is composed of adenosine triphosphate, adenosine diphosphate and a phosphate compound, inorganic phosphoric acid is provided for adenosine diphosphate by catalyzing dephosphorizing of the phosphate compound through the enzyme catalyst, and adenosine triphosphate is regenerated to achieve circulating energy supply. According to the method, a high-activity enzyme combination with a specific source is adopted, the conversion rate of the substrate 2 '-methoxyl-uridine monophosphate is high, the final conversion rate of 2'-methoxyl-uridine triphosphate reaches up to 92.6%, and the method has the advantages of being high in synthesis efficiency, simple in step, environmentally friendly and controllable in cost.

The invention relates to the technical field of biomedicine, and aims to solve the problems of poor drug permeability, drug resistance, drug toxicity, high surgical risk, infection recurrence, donor tissue shortage and the like of prevention and treatment means of fungal keratitis. The invention provides application of a metabolic marker in preparation of a product for diagnosing, preventing or treating fungal keratitis and a screening method of the metabolic marker. The metabolic marker is at least one of adenosine, adenosine monophosphate and uric acid. In fungal cornea, adenosine and adenosine monophosphate are reduced, and uric acid is increased. According to the invention, three differential metabolites are screened based on the combination of non-targeted metabonomics and targeted mass spectrometry detection, and the provided purine metabolic pathway regulation and the level of the three metabolites can be used for diagnosis of fungal keratitis patients and research and development of new therapeutic targets.

Provided is a composition that includes an extract from seaweed and has excellent action and efficacy. This composition is characterized by including extracellular vesicles (EVs) and carotenoids.

Disclosed herein are methods, systems, and compositions for enhancing the effectiveness of β-cell (Beta-cell)-based therapies. Also disclosed herein are methods, systems, and compositions related to identifying, sorting and separating heterogeneous populations of stem cell-derived pancreatic β-cells (sBCs) into more useful and functionally homogeneous cell populations. In many embodiments, the most mature and functional of the sBCs are identified and live-sorted using the cell surface protein Ectonucleoside Triphosphate Diphosphohydrolase-3 (ENTP3), which is also referred to as CD39L3. The presently disclosed methods, systems, and compositions are useful for cell therapies, for example replacement therapy. In many embodiments the disclosed systems, methods, and compositions are useful in treatments for diabetes. In some embodiments, the disclosed methods, systems, and compositions may be useful in treating, preventing, and / or curing diabetes, for example type-1 diabetes.

The present application relates to the field of biological catalysis, and discloses a method for preparing creatine phosphate by immobilized enzyme catalysis, which comprises the following steps: mixing gelatin and a positive charge modifier to obtain a carrier liquid; adding creatine and magnesium salt to a crude creatine kinase solution for pre-incubation to realize conformational locking, then mixing with the carrier liquid and dropping into a low-temperature coagulation bath to solidify into gel microspheres, and then cross-linking and washing to obtain immobilized creatine kinase particles; finally, adding substrates, sodium hexametaphosphate, polyphosphate kinase and immobilized particles into a reaction system for reaction, and then separating and collecting the supernatant to obtain creatine phosphate. The present application protects the three-dimensional conformation of the active center of the enzyme by pre-incubation, uses the positive micro area of the carrier to electrostatically enrich the substrates to accelerate mass transfer, and introduces an auxiliary enzyme to construct an in-situ substrate regeneration system to re-phosphorylate the by-product adenosine diphosphate into adenosine triphosphate. The present application eliminates the feedback inhibition of the product, reduces the cost of raw materials, and improves the overall catalytic efficiency.

The utility model discloses disodium adenosine triphosphate vacuum drying equipment, and belongs to the technical field of disodium adenosine triphosphate. The adenosine disodium triphosphate vacuum drying equipment comprises a drying table, a drying groove is formed in the upper surface of the drying table in the vertical direction, and a first filter plate is slidably connected to the inner wall of the drying groove. Through the cooperation of the drying table, the drying groove, the first filter plate, the second filter plate, the filter holes, the heating pipe, the box cover, the cam, the driving motor, the guide plate and other structures, the adenosine disodium triphosphate raw material can be shaken and dried, and it is ensured that the raw material is fully heated and dried; and the adenosine disodium triphosphate raw material can be fully shaken, so that the drying effect is improved.

The invention provides a kit for detecting pyruvate kinase and application, and belongs to the technical field of pyruvate kinase detection. The kit comprises a cell ATP removal reagent and a pyruvate kinase detection reagent, the cell ATP removal reagent comprises adenylate cyclase; the pyruvate kinase detection reagent comprises a bioluminescent enzyme, a bioluminescent enzyme substrate, phosphoenolpyruvic acid, adenosine diphosphate and a non-PK kinase inhibitor; the non-PK kinase inhibitor is a myokinase inhibitor and / or a nucleoside diphosphate kinase inhibitor. The kit for detecting the pyruvate kinase can be used for qualitatively or quantitatively detecting the PK activity in a sample. When the kit is used for detecting the light-emitting signal generated by the ATP of the PK reaction product, the kit is suitable for long-time detection, and the enzyme activity is in positive correlation with the amount of the ATP. The kit provided by the invention can be used for detecting the enzyme activity of PK in different samples, and has the advantages of high sensitivity, high specificity, good linear relationship and stable fluorescence signal.