The invention provides a biomarker on-site rapid quantitative detection method based on lateral flow immunochromatography and a smartphone, and relates to the technical field of biological detection. According to the method, a smartphone camera and a
macro lens with an
LED lamp light supplementing function are combined to construct a portable reader; the method comprises the following steps: fitting and photographing a T line area of a lateral flow immunochromatography test strip,
processing and analyzing an image through an intelligent
mobile phone application program to obtain a chromatic value of a T line, calculating a concentration value of a biomarker in a sample according to the chromatic value and a pre-established
calibration curve, and finally displaying a result on an interface of the intelligent
mobile phone application program. The method is high in sensitivity, good in
repeatability, high in accuracy, easy and convenient to operate and low in cost, can be widely applied to the fields of
disease diagnosis,
food safety, environment monitoring and the like, and provides a new technical scheme for rapid quantitative detection of biomarkers.