Methods and compositions for expansion and differentiation of skeletal muscle stem cells or progenitor cells

A composition, stem cell technology, applied in the direction of bone/connective tissue cells, biochemical equipment and methods, embryo cells, etc., can solve problems such as micro-efficiency

Inactive Publication Date: 2017-11-28
AGENCY FOR SCI TECH & RES
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The use of myogenic stem cells as cell therapy for various muscle diseases such as skeletal musc

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  • Methods and compositions for expansion and differentiation of skeletal muscle stem cells or progenitor cells
  • Methods and compositions for expansion and differentiation of skeletal muscle stem cells or progenitor cells
  • Methods and compositions for expansion and differentiation of skeletal muscle stem cells or progenitor cells

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Embodiment Construction

[0029] specific implementation plan

[0030] Muscle tissue engineering is an important approach for regenerating functionally deficient muscles. Muscle, such as but not limited to skeletal muscle, is a highly complex and heterogeneous tissue that performs numerous different functions in an organism. The process of building muscle, also known as myogenesis, can be broken down into several distinct stages. During embryonic myogenesis, mesoderm-derived structures generate the organism's first myofibers, and in subsequent waves additional fibers are generated along these initial template fibers. It is known in the art that attempts to mimic the expansion and differentiation of myoblasts in cell culture are fraught with difficulties, for example, the known problem that cells do not respond due to additives and components required for cell survival and expansion. Instead, myoblasts are expanded ex vivo without losing their intrinsic differentiation potential in cell culture. Acco...

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Abstract

The present disclosure describes compositions for preparing a myofiber or myotube from a skeletal muscle stem cell or progenitor cell comprising a carnitine and/or a derivative thereof, a fatty acid a steroid and combinations thereof. Preferred embodiment comprises of 0,1 mM L-carnitine, 0.2 mM 9-cis-linoleic acid and 10 mM dihydrotestosterone. Also disclosed is a composition for inducing expansion of skeletal muscle stem cells or progenitor cells comprising a fibroblast growth factor agonist, a Notch signalling agonist, a nucleic acid, and combinations thereof. Preferred embodiment comprises 20 ng/ml basic fibroblast growth factor (bFGF), 50 [mu]g/ml [Delta]-like ligand 1 (DLL1), 10 mM hypoxanthine and 1.6 mM thymidine.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of Singapore Provisional Application No. 10201501387X filed 25 February 2015, the entire contents of which are incorporated herein by reference for all purposes. technical field [0003] The invention relates to the fields of cell biology, molecular biology and biotechnology. In particular, the invention relates to differentiating, culturing and expanding skeletal muscle progenitor cells in cell culture. Background technique [0004] The use of myogenic stem cells as cell therapy for various muscle diseases such as skeletal muscle injury and cachexia, and in regenerative medicine has been attempted for decades with little success. Myocytes derived, for example, from induced pluripotent stem cells (iPSCs) are promising candidates for stem cell therapy to regenerate skeletal muscle because they allow allogeneic transplantation. Therefore, there remains a need for safe and well-tolera...

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N2500/40C12N2501/115C12N2501/415C12N2501/42C12N2506/02C12N2506/45C12N5/0658C12N2501/999
Inventor 黄仕强
Owner AGENCY FOR SCI TECH & RES
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