44 results about "Xanthosine" patented technology

A method for isothermal DNA amplification comprising: providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing Xanthosine, a second primer at least partially complementary to a region of DNA and containing Xanthosine, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises Xanthosine in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near Xanthosine; and amplifying the DNA substantially without thermal cycling.

The invention relates to IMP (inosine monophosphate) dehydrogenase synthesized by 'Bailing' producing fungus Chinese caterpillar fungus hirsutella sinensis to metabolize guanine nucleotide based on xanthosine monophosphate and coding genes and application of the IMP dehydrogenase. The IMP dehydrogenase comprises proteins shown by SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3, and coding genes of the IMP dehydrogenase correspond to nucleotide sequences shown by SEQ ID No.4, SEQ ID No.5 and SEQ ID No.6. Detailed studies on metabolic pathways of the xanthosine monophosphate synthesizing the guanine nucleotide in principle are carried out and include that cloned DNAs (deoxyribonucleic acids) in the provided nucleotide sequences can be transferred into engineering bacteria by means of transduction, transformation and transconjunction, and high expressivity is given to the host guanine nucleotide by adjusting expressions of guanine nucleotide biosynthetic genes, so that an effective way for increasing output of the guanine nucleotide is provided, and the IMP dehydrogenase and the coding genes have significant application prospects.

The invention relates to a probe and kit for detecting SEPT9 gene methylation and discloses an optimized probe for detecting SEPT9 gene methylation. 2-3 deoxyinosine nucleotide bases (I) are introduced at a specific position on the methylation probe so that the methylation probe can be fully combined with a methylated sample DNA and the existence of a non-methylated site in a combination zone can be tolerated. Therefore, the detection efficiency of SEPT9 gene methylation is effectively increased. The probe can be applied to the kit for detecting SEPT9 gene methylation in combination with SEPT9 and GAPDH gene amplification primers.

The invention discloses a phagostimulant for fish larvae and juveniles of spotted maigre and a preparation method thereof. The phagostimulant comprises the following compositions in portion by weight: 0.05 to 0.1 portion of dimethyl-beta- propionic acid thetine, 0.1 to 0.2 portion of kanirin, 0.1 to 0.3 portion of glycine trimethylpropyl, 20 to 30 portions of inosinic acid, 25 to 35 portions of clamworm extracting solution, 1 to 5 portions of betaine, 10 to 15 portions of lactamine, 8 to 10 portions of glycin, 26 to 28 portions of internal organ powder of cuttlefish, and 1 to 3 portions of lycopene. The preparation method of the phagostimulant comprises the following steps: raw materials are weighed according to weight portions and are mixed, and then the clamworm extracting solution produced by the mixing extraction of alcohol is added into the mixture. The phagostimulant has the advantages that the phagostimulant has small using amount, apparent effect, steady sources, low price, and simple production technology, can effectively improve survival rate, reduce the waste of baits, can also improve the high temperature resistance and the anti-anoxia ability of fish, strengthen the endurance of the fish to the change of water environment, and can improve the meat quality of breed varieties.

The invention discloses burdock oral liquid for beautifying and removing freckles. The burdock oral liquid for beautifying and removing freckles comprises the following raw materials in parts by weight: 10 to 50 parts of burdock, 10 to 20 parts of radix polygonati officinalis, 10 to 20 parts of resina draconis, 10 to 20 parts of angelica sinensis, 10 to 20 parts of pseudo-ginseng, 10 to 20 parts of rhizoma bletillae, 10 to 20 parts of poria cocos, 10 to 20 parts of salvia miltiorrhiza, 10 to 20 parts of raspberry, 10 to 20 parts of sweet almond, 10 to 20 parts of cortex mori, 10 to 20 parts of sealwort, 10 to 20 parts of curcuma aromatica, 10 to 20 parts of rhizoma cyperi, 10 to 20 parts of astragalus membranaceus, 10 to 20 parts of coix seed, 10 to 20 parts of rhizoma atractylodis macrocephalae, 10 to 20 parts of albizia julibrissin, 10 to 20 parts of leonurus, 10 to 20 parts of caulis spatholobi, 10 to 20 parts of white muscardine silkworm, 10 to 20 parts of glossy privet fruit, 10 to 20 parts of radix angelicae, and 10 to 20 parts of kaladana. According to the burdock oral liquid for beautifying and removing freckles, the burdock has the effects of promoting blood circulation and preventing cardiovascular disease; a burdock extract has good effects on removing free radicals and resisting oxidation; xanthosine, amino acid and amino acid synthesis collagen in burdock show remarkable effects on beautifying body, inhibiting melanin growth, and keeping the skin bright and elastic.

The invention discloses a group of substituted benzoheterocycle amine derivatives and a preparation method and related application thereof as an IMPDH (inosine monophosphate dehydrogenase) inhibitor. The IMPDH inhibitor has good application prospects in virus resistance, immunosuppression, tumor resistance, bacterium resistance, parasite resistance and the like. In the invention, a new-structure IMPDH inhibitor shown by the formula (I) is obtained through the design, synthesis and activity screening study on an active compound targeting IMPDH, and a foundation is laid for the development and application of the compounds as the medicines and medicinal compositions thereof with related effects such as virus resistance, tumor resistance, immunosuppression and the like.

The invention discloses a feeding promoting agent of sebastodes fuscescens, the ingredients and the weight percentage of which are: 35 to 45 percent of betaine, 25 to 35 percent of inosinicacidIMP, 15 to 20 percent of dimethyl-Beta-propiothetin, 8 to 15 percent of glycine and 5 to 10 percent of sodium citrate; the feeding promoting agent is prepared by that various raw materials used for preparing the feeding promoting agent are dried, grinded, weighed accurately according the dosage and mixed fully and evenly with the coefficient of variation being less than or equal to 5 percent; the feeding promoting agent of feedstuff is applied to the compound feedstuff of the sebastodes fuscescens by the proportion of 0.5 (weight percentage), and can promote the ingestion and the growth of the sebastodes fuscescens with 82.06 percent of the rate of body weight gain, 1.29 percent of specific growth rate and 2.81 percent of ingestion rate; meanwhile, the used raw materials have stable source, low price, simple manufacturing technique and little waste feedingstuff, reduce remnant feed, is beneficial to environmental protection and can carry out the production and the aquaculture of the sebastodes fuscescens on large scale.

The present invention relates to a microorganism with improved production of 5′-xanthosine monophosphate and 5′-guanine monophosphate, and more specifically, to a Corynebacterium sp. microorganism having increased proline dehydrogenase activity compared with an intrinsic activity thereof, a method for producing 5′-xanthosine monophosphate or 5′-guanine monophosphate from the culture medium obtained by culturing the transformed microorganism, and a use of the microorganism for production of 5′-xanthosine monophosphate or 5′-guanine monophosphate.

The invention relates to a gene mutation detection primer and application, the primer includes an anchor sequence, a purine ring and a detection sequence, the anchor sequence at the is located at the primer 5'- end, forms more than 14 bases of complementary with a target sequence, the purine ring is formed as follows: 3-4 xanthosine, inosine or deoxidized inosine forms a bubble ring at the 5th-8th base position of the primer 3'- end, and the detection sequence is located at the primer 3'-end and is formed by 5-8 bases. According to the technical scheme of the primer, the bubble ring is inserted at the 5th-8th base position from the primer 3'- end, and the bubble ring is formed by '0' or '1' base counterpoint with a corresponding position of the target sequence, and the primer is given highly specific gene mutation detection ability, so that the primer can inhibit nonspecific amplification, improve the detection capability, and can be applied to the detection of low copy gene mutation detection, multiplex PCR (polymerase chain reaction), gene chip and other fields.

The invention discloses an immunopotentiator for an adult portunus trituberculatus and an application thereof. The immunopotentiator is characterized by being prepared by evenly mixing the following raw materials in parts by weight: 5-10 parts of levamisole, 15-20 parts of astaxanthin, 10-20 parts of hypoxanthine nucleotide, 20-30 parts of beta-1,3-glucan, and 30-40 parts of seaweed polysaccharides. The mass percentage content of the immunopotentiator in a feeding feed is 0.06-0.08%. The immunopotentiator has the advantages that: the addition amount of the immunopotentiator in the feed is only 0.06-0.08%, thus the immunity and the disease resistance of the adult portunus trituberculatus are significantly improved, so as to improve the survival rate of breeding portunus trituberculatus, wherein the survival rate can be increased by 26% or more, and the immune protection rate can reach 59% or more; and the immunopotentiator has no toxic or side effect, no residue and no drug resistance, also has no adverse effect on the feed palatability, has less addition amount, and can significantly improve the efficacy of the immunity and the disease resistance of the adult portunus trituberculatus.

The invention relates to a novel use of small molecule metabolites such as acetyl leucine, propionyl carnitine, butyryl carnitine, kynuric acid, xanthine nucleoside, 5-hydroxy-L-tryptophan, p-cresol sulfate and tryptophan as combined markers in a urine sample in preparation of a kit for diagnosing prostate cancer patients in subjects. The invention also relates to a kit for diagnosing prostate cancer patients in subjects. Through detection of the relative concentrations of the combined markers in urine samples of male subjects, based on a binary logistic regression equation, the combined marker variables and determined section values are calculated and it is determined that the subjects are prostate cancer patients or not. Through combination of the small molecule metabolites, the kit canbe used for auxiliary diagnosis of prostate cancer and especially for diagnosis of prostate cancer in the clinical diagnostic ash area.

The invention relates to an adenosine monophosphate (AMP) adenosine deaminase for synthesizing and metabolizing an inosine monophosphate from adenine nucleotide of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application thereof. The AMP adenosine deaminase comprises the proteins shown in SEQ ID No. 1 and SEQ ID No. 2, and the coding gene of the AMP adenosine deaminase corresponds to the nucleotide sequence shown in SEQ ID No. 3 and SEQ ID No.4. According to the invention, the metabolizing way for synthesizing the inosine monophosphate from the adenine nucleotide is researched in detail from the principle, and the cloned DNA containing the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation, and combined transfer; the expression of the gene is biologically synthesized by regulating the inosine monophosphate, the host inosine monophosphate is endowed with high expressivity, an effective way is provided for increasing the yield of the inosine monophosphate, and the invention has a great application prospect.

The invention relates to a probe group for diagnostic gene methylation and application of the probe group, and discloses a preparation method of an optimized gene methylation probe. The optimized methylation detection probe is obtained by introducing a dideoxyinosine nucleotide base to the specific position of a methylation probe. The optimized methylation detection probe can be combined with completely methylated sample DNA, and can tolerate 1-2 non-methylated sites in a binding region, so that the detection efficiency of incompletely methylated samples is effectively improved.

The disclosed six-membered carbocyclic nucleoside analogues comprise: adenosine analogue, guanosine analogue, carnine analogue, mercaptopurine riboside analogue, cytidine analogue, 5-fluorocytidine analogue, uridine analogue, 5-fluorouridine analogue, and thymidine analogue as well as their acceptable salts formed by equimolar acid in pharmacy. Wherein, the opposite five-step synthesis method using the pinitol, acetone, methane sulfonyl chloride, p-toluenesulfonyl chloride, benzene sulfochloride, and nucleoside base as materials; the pyridine, water, glacial acetic acid, absolute methanol, DMSO, N, N-DMF as the solvent; the p-toluenesulfonic acid, 2, 2-dimethoxylpropane, anhydrous NaSO4, NaHCO3, triethylamine, and anhydrous K2CO3 as the catalyst. This invention restrains specially the replication of HIV and herpesvirus.

A composite medicine for treating hepatism is prepared from scutelloside (60-2000 Wt portions) and glycyrrhizic acid or its pharmacologically acceptable salt (10-800). Its preparing process is also disclosed.

The invention provides application of hypoxanthine, hypoxanthine riboside, xanthine, xanthosine or any combination thereof in preparation of medicines for treating tumors, and belongs to the technical field of medicines. The invention further comprises application of a combination of the hypoxanthine, the hypoxanthine riboside, the xanthine, the xanthosine or any combination of the hypoxanthine, the hypoxanthine riboside, the xanthine and the xanthosine and one or more of a human basic group, nucleoside or deoxynucleoside. The hypoxanthine, the hypoxanthine riboside, the xanthine, the xanthosine or any combination thereof can be applied to chemical treatment of malignant tumors of liver cancer, lung cancer, esophagus cancer, gastric cancer, colon cancer, pancreatic cancer, prostate cancer, leukemia, promyelocytic leukemia, breast cancer, ovarian cancer, cervical cancer or T-cell lymphoma and the like, has an obvious antitumor effect, is free of myelosuppression or an important organ damage at a normal dosage, and has the antitumor effects of high efficiency, broad spectrum and low toxicity.

The invention relates to an inosine monophosphate (IMP) dehydrogenase from 'Bailing' producing strain Cordyceps sinensis Hirsutella sinensis for participating in anabolism on IMP to obtain a xanthylic acid, a gene for encoding the dehydrogenase and application of the dehydrogenase. The IMP dehydrogenase comprises a protein shown by SEQ ID No.1 and a protein shown by SEQ ID No.2, and the coding gene of the protein shown by SEQ ID No.1 and the coding gene of the protein shown by SEQ ID No.2 respectively correspond to a nucleotide sequence shown by SEQ ID No.3 and a nucleotide sequence shown by SEQ ID No.4. According to the invention, the metabolic pathway of the IMP to synthetize the xanthylic acid is traversed from the principle; the cloning deoxyribonucleic acids (DNA) of the nucleotide sequences provided by the invention can be transferred into engineering bacteria through a transduction method, a transformation method and a conjugal transfer method; and through regulating the expression of the biosynthetic gene of the xanthylic acid, the high expressivity of a host xanthylic acid is endowed, and an effective way for increasing the yield of the xanthylic acid is provided. The IMP dehydrogenase has a great application prospect.

The invention belongs to the technical field of medicine, and particularly relates to application of hypoxanthine nucleotide in preparation of anti-infective drugs. Experiments prove that the hypoxanthine nucleotide can significantly improve the sensitivity of Escherichia coli, Klebsiella pneumoniae, staphylococcus aureus multi-drug-resistant bacteria, pseudomonas aeruginosa and other bacteria toamoxicillin, cefoperazone, meropenem, gentamicin and other antibiotics, so that the hypoxanthine nucleotide can be used as an anti-infective drug together with antibiotics. Bacteria are killed under the condition of low-concentration antibiotics, a good anti-infection effect is achieved, and meanwhile bacterial drug resistance is reduced.

The invention relates to the technical field of biology, in particular to a PCR (Polymerase Chain Reaction) product qualitative and semiquantitative detecting kit (color PCR kit). The kit comprises a plurality of reagents, such as inosine monophosphate, xanthine oxidase, iodine chloride nitro-tetrazole, hypoxanthineguanine phosphoribesyltransferase, pyrophosphoric acid, and the like. The invention can be used for qualitatively or semiquantitatively detecting a PCR product.

The invention provides application of a purine metabolism marker in preparation of a reagent for screening and diagnosing acquired drug resistance of a lung cancer molecular targeted drug. The purine metabolism marker is one or more of adenosine monophosphate, guanosine monophosphate, inosine monophosphate, xanthosine monophosphate, adenosine, guanosine, inosine, xanthosine, adenine, guanine, hypoxanthine, xanthine and uric acid. The metabolic marker has good diagnostic potential for acquired drug resistance of the lung cancer molecular targeted drug, can be used for diagnosis and curative effect monitoring of acquired drug resistance of the lung cancer molecular targeted drug, and has good guiding significance for development of subsequent clinical application research.

The invention relates to a compound nucleotide immunoenhancer for paralichthys olivaceus, which is composed of the following substances in percentage by weight: 55-65% of cytidylate, 5-15% of uridine monophosphate, 5-15% of thymidine phosphorylase, 1-5% of hypoxanthine nucleotide and 10-20% of bioactive peptide. The compound nucleotide immunoenhancer disclosed by the invention is capable of obviously improving the ingestion and the growth of paralichthys olivaceus, and remarkably improving the cellular immunity, the humoral immunity, the immune gene expression quantity, the disease resistance and the like of paralichthys olivaceus. Moreover, the compound nucleotide immunoenhancer can also be directly added in a feed for paralichthys olivaceus, and orally fed; and the compound nucleotide immunoenhancer has the advantages of being free from toxic and side residues, free from drug resistance, and pollution-free to environment.

The invention belongs to the field of gene engineering and relates to a gene mutation recombination method and application thereof in construction of protein (proteinase) gene mutation library. In a polymerase chain reaction (PCR) system for amplifying a target sequence, nucleotide analogue 2-deoxyinosine-5-triphosphoric acid (dITP) is randomly doped in a target gene order by a PCR reaction so as to generate multi-point random mutation, the endonuclease V (endo V) cutting the dITP is distinguished by specificity so as to segment the amplified target gene order, and then the obtained the mutation target gene segments are extended and recombined by the PCT without a primer, and finally the mutation gene library of the recombined mutation gene is obtained. The method of the invention overcomes the shortage that the traditional DNA shuffling method is not easily controlled and wastes time and labour in the process of randomly cutting the target sequence by endonuclease (DNase I). The method can be widely applied to the construction of random mutation library of all protein (proteinase) coding genes.

The present invention relates to the field of pharmaceuticals and provides the application of 3'-deoxyhypoxanthine nucleosides in the preparation of foods, medicines or health products for the prevention or treatment of food-borne obesity or hyperlipidemia provides, provides the application of 3'-deoxyhypoxanthine nucleosides in the preparation of foods, medicines or health products for the prevention of hypertension or arteriosclerosis, and also provides the application of 3'-deoxyhypoxanthine nucleoside in preparation of skin care products. A study of that present invention show that the 3'-deoxyhypoxanthine nucleosides have significant therapeutic effect on hyperlipidemia, reduces the body weight of foodborne obesity, and overcomes the existing technical bias. And the 3'-deoxyhypoxanthine nucleosides have a more comprehensive efficacy in the prevention or treatment of hyperlipidemia than the existing drugs. And the 3'-deoxyhypoxanthine nucleoside can effectively prevent the occurrence of hypertension and arteriosclerosis by decreasing the content of lipid and high density lipoprotein in serum.

The invention provides a nucleoside compound and a preparation method thereof. The nucleoside compound comprises a compound as shown in a general formula, and pharmaceutically acceptable salts. According to the nucleoside compound, an amino substituent group at the sixth site of cordycepin is changed into aromatic substituent groups, such as p-methylthiophenyl, phenoxyl and p-methylaniline, so that a purine nucleoside metabolic pathway can be effectively avoided in vivo, the dosage concentration of the medicine is kept, the active effect of the medicine is fully exerted, and the defect that the generated medicine is quickly deaminated under the action of adenosine deaminase to become an inactive metabolite, namely 3'-deoxyinosine, is overcome.