Method for measuring royal jelly

A determination method and technology of royal jelly, applied in the direction of testing food, measuring devices, instruments, etc., to achieve the effect of high sensitivity and simple analysis method

Inactive Publication Date: 2008-12-10
BEE RES INST CHINESE ACAD OF AGRI SCI
View PDF0 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is currently no reliable met

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring royal jelly
  • Method for measuring royal jelly
  • Method for measuring royal jelly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] ATP, ADP, AMP, IMP, HxR, Hx, adenine and adenosine standard substance (purchased in Sigma company) are dissolved in ultrapure water, final concentration is 50ppm, measure with liquid chromatography, obtain standard liquid chromatography, see figure 1 .

[0031] Get newly harvested rapeseed royal jelly sample 0.5g, add 5ml 5% HClO 4 , mixed well, and centrifuged at 12000rpm for 10min; take the supernatant, add 700μL 6mol / L KOH and 50μL 2mol / L Na 2 CO 3 , after fully mixing, centrifuge at 12000rpm for 10min, filter the supernatant with a 0.45μm filter membrane, and 2 HPO 4 As the mobile phase, the content of each component is measured with a liquid chromatograph, and the obtained liquid chromatogram is shown in figure 2 .

[0032] Compare figure 1 and figure 2 Apparently, the characteristic peaks of Hx and adenine are not detected in the newly harvested royal jelly, indicating that Hx and adenine are hardly contained, while the characteristic peaks of AMP and IMP...

Embodiment 2

[0037] Get each 0.5g of rape royal jelly samples stored at -18°C for 60 days, 4°C for 60 days, 16°C for 60 days and 28°C for 60 days, and add 5ml of 5% HClO 4 , mixed thoroughly, and centrifuged at 12000rpm for 10min. Take the supernatant, add 700 μL 6mol / L KOH and 50 μL 2mol / L Na 2 CO 3 , after fully mixing, centrifuge at 12000rpm for 10min, filter the supernatant with a 0.45μm filter membrane, and 2 HPO 4 As the mobile phase, it is determined with a liquid chromatograph (see image 3 , 4 , 5 and 6).

[0038]It can be seen that with the increase of storage temperature, the contents of IMP, ATP, ADP, and AMP of royal jelly decreased gradually, while the contents of adenine and adenosine gradually increased, and the contents of Hypoxanthine (Hx) and Inosine (HxR) decreased first, and then remained stable . It is basically consistent with the aforementioned degradation mechanism of the inventor. The ATP content of royal jelly stored at 28°C for 60 days was 0, indicating ...

Embodiment 3

[0044] Get each 0.5g of camellia royal jelly samples stored at 4°C for 15 days, 30 days, 60 days and 90 days, adopt the method of Example 1, add 5ml 5% HClO 4 , mixed thoroughly, and centrifuged at 12000rpm for 10min. Take the supernatant, add 700 μL 6mol / L KOH and 50 μL 2mol / L Na 2 CO 3 , after fully mixing, centrifuge at 12000rpm for 10min, filter the supernatant with a 0.45μm filter membrane, and 2 HPO 4 As the mobile phase, it was determined with a liquid chromatograph. The contents of ATP and its degradation products in royal jelly were calculated, and the results are shown in Table 2.

[0045] Table 2 Degradation product content (mg / kg) and F value of camellia royal jelly stored at 4°C for different time

[0046]

[0047] It can be seen that with the increase of storage time, the contents of ATP, ADP, AMP, and IMP of royal jelly decreased gradually, while the contents of adenine and adenosine gradually increased, and the contents of Hypoxanthine (Hx) and Inosine ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a freshness detecting method used for royal jelly. In the specific detecting method, the contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), single phosphoric acid adenosine (AMP), inosinic acid (IMP), hypoxanthine riboside (HxR), hypoxanthine (Hx), adenine and adenosine of the royal jelly are quantitatively determined through methods of liquid chromatography or ultraviolet spectrometry, and the like; the ratio (F value) between the accumulation amount of adenine, adenosine, HxR and Hx and the sum of the qualities of the eight substances (degradation products of ATP and nucleic acid)is calculated; the F value of the royal jelly is used for detecting the freshness of the royal jelly and judging the quality of the royal jelly. Compared with a present royal jelly quality detecting method, the freshness detecting method can judge the quality and the freshness of the royal jelly more accurately and sensitively, can improve the quality standard of the present royal jelly and effectively monitor the quality of the royal jelly.

Description

technical field [0001] The invention relates to a food quality detection method, in particular to a method for measuring the freshness of royal jelly. Background technique [0002] Royal jelly is secreted by the hypopharyngeal glands and mandibular glands of the feeding bees, and is used to feed the queen bee and 1-3 day-old bee larvae. It plays an important role in the hierarchical differentiation of bees. Royal jelly is rich in biologically active substances, and has nutritional and health effects on the human body such as anti-fatigue, anti-bacterial and anti-inflammatory, anti-tumor, lowering blood pressure, and promoting growth. It has high application value in the fields of health food, medicine, and cosmetics. The excellent nutritional and health effects make royal jelly and its products popular all over the world. With the improvement of domestic people's living standards and the growth of health care needs, the domestic royal jelly market is gradually growing. [...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/02G01N30/86G01N21/00
Inventor 吴黎明薛晓锋赵静李熠彭文君胡福良
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap