67 results about "Fluorescent particle" patented technology

The invention relates to the technical field of flow measurement, in particular to a sewage pipeline flow measurement device and method based on dynamic image analysis, and the device comprises a rod body, a control system, a particle release module, a track acquisition module and an image and motion analysis module; the control system is arranged at the top end of the rod body; the track acquisition modules are arranged at the bottom of the rod body; the particle release module is used for storing fluorescent particles and releasing the fluorescent particles into the sewage pipeline to be detected under the control of the control system; the track acquisition module is used for shooting the interior of the sewage pipeline to be detected to obtain an image containing fluorescent particles; the image and motion analysis module is used for analyzing the motion characteristics of the fluorescent particles released in the sewage pipeline to be detected so as to determine the flow of the sewage pipeline to be detected. Thus, the bottom of the device is placed in the sewage pipeline to be detected, the motion characteristics of the fluorescent particles are determined by shooting the tracks of the fluorescent particles, the flow of the sewage pipeline is determined, and the device is efficient, accurate and low in operation difficulty.

Provided is a temperature-responsive fluorescent particle comprising at least one type of fluorescent molecule in a molecular assembly comprising and constituted by at least one type of amphiphilic molecule, wherein the fluorescent molecule emits fluorescence when the molecular assembly is in the liquid phase and it is quenched when the molecular assembly is in the solid phase, due to a temperature-responsive solid-liquid phase transition, so that fluorescence emission and quenching of the fluorescent molecule are reversibly switched in a temperature responsive manner. Also provided is a temperature-responsive fluorescent probe comprising the temperature-responsive fluorescent particle the surface of which is modified with a biomolecule recognition element, and methods for detecting and quantitatively determining a biomolecule with the temperature-responsive fluorescent probe.

The application discloses a kind of color fluorescent particles for electrophoretic display, it is related to the field of photoelectric display, color fluorescent particle is core-shell structure, color fluorescent particle includes successively from inside to outside: core, intermediate layer and shell layer;Core is pigment unit, intermediate layer is quantum dot layer coated on the surface of pigment particle, shell layer includes high molecular polymer selected from one or more of polymethyl methacrylate lauryl, polymethyl methacrylate, and is constituted, shell layer is coated on the surface of intermediate layer;Color fluorescent particle contains intrinsic base color presented by pigment unit and compensation fluorescence excited by quantum dot layer receiving light;Compensation fluorescence is compatible with the color of intrinsic base color.In addition, the application also provides the preparation process of color fluorescent particle.The color fluorescent electrophoretic particle provided by the application is pure and full of color, and the color is more brilliant under ultraviolet excitation.

The purpose of one aspect of the present invention is to provide fluorescent particles having excellent dispersibility. One aspect of the present invention relates to a fluorescent particle (100) having: a core particle (10A) with a first charge, the first charge being a positive charge or a negative charge; at least one polymer layer (12) and at least one fluorescent layer (11A, 11B) on the core particle, the polymer layer containing a polymer and carrying a second charge, the second charge being a positive charge or a negative charge; and a fluorescent layer containing metal nanoclusters (13) having fluorescent properties and carrying a third charge, the sign of which is opposite to that of the second charge.

The invention discloses a preparation method of traceable polyester staple fibers, which comprises the following steps: mixing a tracing raw material and a renewable synthetic fiber raw material, and carrying out melt spinning to obtain the traceable regenerated polyester staple fibers, the tracing raw material comprising at least two of cobalt Prussian blue analogues, germanate fluorescent materials and metal oxides. The invention also provides a traceable polyester staple fiber product prepared by the preparation method. According to the method, two color changes under thermal stimulation and solvent stimulation of primary-color polyester fibers can be achieved, meanwhile, the tracing germanate fluorescent particles and the metal oxide tracing particles are added to make up for the defect that color changes are difficult to recognize after the fibers are dyed, and meanwhile the tracing means is complicated, so that the fibers are difficult to counterfeit.

The invention discloses an in-vivo ferrofluid drug transport performance testing device and a testing method. The device comprises a mounting table, a ferrofluid loading device and a ferrofluid loading device, the test board is mounted on the mounting table, a test substrate is arranged on the test board, the test substrate is used for placing ferrofluid droplets, and the ferrofluid droplets are wrapped with fluorescent particles; the moving module is mounted on the mounting table, the moving module is provided with a supporting column capable of moving front and back, the supporting column is provided with a movable table capable of moving up and down, and a magnetic block is arranged on the movable table and located below the test table; a shooting area of the camera faces the test substrate; and a light-emitting area of the excitation light source faces the test substrate. Compared with the prior art, the technology has the advantages that quantitative determination of the Roussonii instability critical magnetic field is realized, a reliable basis is provided for optimizing magnetic field parameters, and the leakage rate of the nano-drug in the targeted transportation process is accurately quantified by shooting and analyzing the residual path of the fluorescent particles during targeted transportation of the ferrofluid droplets; the method has the advantages of high repeatability, reliable detection and the like.

The invention discloses a cross-scale fluorescent particle detection device and method and application, and belongs to the technical field of cell biology.Wavelength multiplexing light spots (small light spots formed by short-wavelength laser excite small particles, and large light spots formed by large-wavelength laser excite large particles) are combined with confocal configuration and a phase-locked amplification principle, and the cross-scale fluorescent particles are detected in a rectangular-section capillary tube (lt; according to the method, the size limitation of traditional flow type detection is broken through, channel blockage is avoided, and a key technical support is provided for research on a blood coagulation mechanism and tumor metastasis.

The application provides a fluorescent structural color anti-counterfeiting material and a preparation method thereof, and the preparation method comprises the following steps: preparing composite fluorescent particles of mesoporous silica microsphere loaded halogen perovskite fluorescent particles CsPbX3, wherein X is Br, Cl or I; mixing the composite fluorescent particles with monodisperse silica nanoparticles, resin, a solvent, an initiator and an additive to obtain a mixture, wherein the volume percentage of each component in the mixture is as follows: 25-65% of the nanoparticles, 35-75% of the resin, 0.01-10% of the initiator, and 5-90% of the solvent, and the mass of the composite fluorescent particles is 0.01-3% of the mass of the silica nanoparticles; performing rotary evaporation on the mixture under vacuum to obtain concentrated paint, and after photocuring or thermal curing, the anti-counterfeiting material is obtained. The anti-counterfeiting material of the technical scheme has the dual anti-counterfeiting effects of structural color anti-counterfeiting under natural light and fluorescent anti-counterfeiting under ultraviolet excitation.

The application provides a kind of dual-mode acoustic contrast agent with targeting and its preparation method and application, belong to the technical field of ultrasound contrast agent preparation. By compounding ultrasound contrast agent and fluorescent particles, a dual-mode acoustic contrast agent including ultrasound contrast agent and fluorescent probe containing DBCO structure is formed. The dual-mode acoustic contrast agent has targeting, and the DBCO group on the surface of the fluorescent probe can react with Ac4ManNAz to form an azide group enriched in tumor tissue, thereby realizing significant targeted fluorescence imaging of tumors, which is beneficial to early detection of tumors. The dual-mode acoustic contrast agent has both ultrasound imaging and targeted fluorescence imaging functions, with high imaging sensitivity and strong specificity, and has good application prospect.

The invention relates to the technical field of anti-corrosion coatings, in particular to an anti-corrosion early warning coating, a preparation method and application of the anti-corrosion early warning coating to environment monitoring equipment. The corrosion-resistant early-warning coating comprises an inner-layer coating and an outer-layer coating, wherein the outer-layer coating is prepared from the following components: a water-based fluorocarbon emulsion, a reinforcing agent, a polyether siloxane flatting agent and water; the inner-layer coating is prepared from the following components: waterborne epoxy emulsion, fluorescent particles, a waterborne amine curing agent, a polyether siloxane flatting agent and water. According to the double-layer structure coating prepared through the technical scheme, through the synergistic effect of the innovative reinforcing agent and the fluorescent particles, the excellent acid resistance is achieved, and the adhesive force can reach 26.5 MPa to the maximum; meanwhile, whether the surface protection state of the environment monitoring equipment is intact or not can be studied and judged immediately.

To provide a light-emitting device that can improve yield during manufacturing while achieving wide-angle light distribution.SOLUTION: A semiconductor light-emitting device includes: a substrate; a light-emitting element that is provided on one principal surface of the substrate and includes a light-emitting layer comprising a semiconductor; a phosphor layer that is formed at the one principal surface so as to cover the light-emitting element, that has a frame-like part protruding to the side along the one principal surface from a lower end of a side surface and continuously formed at a circumferential edge of the phosphor layer as seen in a top view, and that includes phosphor particles excited by light emitted from the light-emitting layer and emits fluorescence; a light-transmitting sealing layer that covers an upper surface of the phosphor layer, that extends from an upper end of the side surface so as to cover the side surface, and that stops at an upper surface of the frame-like part; and a transmission and reflection layer that is formed across an upper surface of the sealing layer and that transmits a portion and reflects a portion of each of light emitted from the light-emitting layer and fluorescence emitted from the phosphor layer.SELECTED DRAWING: Figure 3

This invention discloses an automated monitoring and detection cascade control system for bioaerosols, comprising a cascaded monitoring and detection unit and an automatic control unit. The cascaded monitoring and detection unit includes: a first-level monitoring module, a bioaerosol monitor based on laser-induced fluorescence technology (aerosol fluorescent particle monitor); a second-level monitoring module, a bioaerosol live-cell monitor based on ATP fluorescence detection technology (aerosol live-cell ATP monitor); and a third-level detection module, a bioaerosol nucleic acid detector based on microfluidic PCR technology (an integrated bioaerosol detection and nucleic acid detection system). The automatic control unit uses high and low level switching signals to achieve sequential instantaneous triggering of the three levels of equipment, completing automated hierarchical monitoring and early warning of "broad-spectrum monitoring → biological confirmation → specific detection". This invention solves the problem in existing bioaerosol monitoring technologies that cannot simultaneously achieve broad-spectrum, real-time, accurate, and automated monitoring.

This disclosure provides a method for detecting an analyte in a sample, wherein the sample is introduced into an analytical chamber along with emulsion droplets or gel beads. In another aspect, this disclosure provides designs for formulating emulsion droplets or gel beads such that they can be used for the large-scale parallel detection of analytes. Formulations containing specific combinations of fluorescent particles allow for the optical determination of the identity of each fluorescent particle. These combinations are based on the particle fluorescence emission wavelength, fluorescence excitation wavelength, and particle count.

The utility model discloses a wavelength conversion assembly and lighting equipment. The wavelength conversion assembly comprises a supporting piece, a reflection unit and a fluorescent glass film. Wherein the reflection unit comprises a first reflection layer and a second reflection layer which are used for reflecting light; the first reflecting layer is connected between the second reflecting layer and the supporting piece, and the thermal conductivity of the first reflecting layer is larger than that of the second reflecting layer. The fluorescent glass film covers the side, away from the supporting piece, of the second reflecting layer and is suitable for being arranged on the light path where the exciting light is located. The fluorescent glass film provided by the utility model is a fluorescent film in which a glass matrix contains fluorescent particles. Therefore, the fluorescent film arranged on the wavelength conversion assembly adopts a glass matrix inorganic packaging mode, and compared with a mode of organic packaging by organic silica gel or resin and the like, the fluorescent glass film has better heat-conducting property, so that the phenomenon that the fluorescent glass film possibly becomes yellow under long-time irradiation of high-power laser is avoided, and the service life of the fluorescent glass film is prolonged. And the excitation efficiency of the excited light is ensured.

The utility model discloses a wavelength conversion assembly and lighting equipment. The wavelength conversion assembly comprises a supporting piece, a first wavelength conversion layer and a second wavelength conversion layer. Wherein the first wavelength conversion layer comprises a bearing part and a protruding part which are connected with each other; the bearing part is arranged on the supporting piece, and the side, away from the supporting piece, of the bearing part is provided with a first area and a second area, the protruding part is located in the first area and protrudes relative to the bearing part. The second wavelength conversion layer is wound on the periphery of the protruding part and covers the second area; wherein the second wavelength conversion layer comprises red fluorescent particles. The second wavelength conversion layer is doped with the red fluorescent particles, so that the specified laser can be converted into red fluorescence under the excitation of the red fluorescent particles. When the wavelength conversion assembly is configured in the lighting equipment, the lighting light generated by the lighting equipment can be mixed with the red light component, so that the color rendering index of the lighting light is improved, and the lighting equipment can meet the application scene with the high color rendering index.

The application discloses a fluorescent particle induced ultra-transmittance radiation refrigeration film and a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing DCPDA monomers, graphene quantum dot dispersion liquid and an ultraviolet curing agent to obtain a mixed solution; concentrating the mixed solution to obtain a casting solution; coating the casting solution on the surface of a substrate to obtain a wet film; and curing the wet film to obtain the fluorescent particle induced ultra-transmittance radiation refrigeration film. By introducing the fluorescent particles into a polymer matrix and adding the ultraviolet curing agent, the fluorescent particle induced ultra-transmittance radiation refrigeration film is formed, the high transmittance of sunlight and the high infrared emissivity are realized, the radiation refrigeration performance of the fluorescent particle induced ultra-transmittance radiation refrigeration film is excellent, and the fluorescent particle induced ultra-transmittance radiation refrigeration film is suitable for building energy saving, photovoltaic cell cooling and cold chain transportation and the like. The preparation process is simple and suitable for large-scale application.

α-Sialon phosphor particles containing Eu, wherein at least one slit is formed on a surface of the α-sialon phosphor particle, wherein the distance from the surface of the α-sialon phosphor particle to the bottom of the slot is equal to or greater than 200 nm and equal to or less than 1500 nm in at least one cross-section of the slot, wherein the α-sialon phosphor particle is formed from an α-sialon phosphor containing an Eu element, represented by the general formula: (M1 x , M2y,Eu z ) (Si 12-(m+n) Al m+n )(O n N 16-n ) (assuming that M1 is a monovalent Li element and M2 is a divalent Ca element), and in the general formula x = 0, 0 < y < 2.0, 0 < z ≤ 0.5, 0 < x + y, 0.3 ≤ x + y + z ≤ 2.0, 0 < m ≤ 4.0 and 0 < n ≤ 3.0 are satisfied, and wherein the α-sialon phosphor particle is produced by a process comprising: Mixing of raw materials containing an element that forms a Eu-containing α-sialon phosphor particle; Heating a mixture of the raw materials to obtain an α-sialon phosphor; Pulverizing the α-sialon phosphor obtained by heating to obtain the α-sialon phosphor particle; and Subjecting the α-sialon phosphor particle obtained by pulverization to an acid treatment to form the slit on the surface of the α-sialon phosphor particle, wherein the acid treatment is carried out by stirring the α-sialon phosphor particle obtained by pulverization in an aqueous acid solution at a stirring speed of 400 rpm or more, wherein the aqueous acid solution is an aqueous hydrofluoric acid solution or an aqueous mixed acid solution obtained by mixing hydrofluoric acid and nitric acid, and where the stock solution concentration of the aqueous acid solution is equal to or higher than 0.7% and equal to or lower than 100%.

The present application is directed towards a method of tracking fluorescence in animals using fluorescent particles.

The invention provides a biomarker multi-joint detection method based on single-wavelength fluorescence coding, and belongs to the technical field of biomarker detection. The method comprises the following steps of: performing fluorescence amplification and digital coding on a biomarker by using processed single-wavelength fluorescent particles through a sample adding area of a sample in an arrayed coating plate type platform; a plurality of biomarkers in a sample can be further quantitatively analyzed through a fluorescence microscopic imaging system (non-flow fluorescence detection and a multi-fluorescence channel module) in combination with image recognition and data analysis software; the multiplex detection technology has the advantages of low cost, simplicity in use, medium and high throughput and the like, and shows huge advantages in the field of biotechnology.

The invention discloses a non-physical isolation digital ELISA detection method based on a fluorescent microsphere probe, a kit and application, and belongs to the technical field of biomedical detection. The method depends on a high-brightness fluorescent microsphere probe, realizes single-molecule immune recognition visualization and fluorescent particle digital counting under the condition of no physical isolation structure, simplifies system configuration based on an open type flat immune chip, improves the performance of a detection platform, overcomes the limitation of physical isolation type digital ELISA, is highly compatible with common equipment, and is suitable for large-scale popularization and application. The adaptation and popularization cost is reduced, and the portability and adaptability are good. High-brightness silicon-based fluorescent microspheres doped with fluorescent nanoparticles are adopted, so that high-sensitivity detection is realized; and establishing a database by using a standard product to realize absolute quantitative analysis. The kit takes a fluorescent silicon microsphere probe as a carrier, target protein can be accurately captured, non-specific adsorption is reduced, the function division of an open type flat chip is clear, and the operation is simple and convenient; the traditional detection sensitivity limitation can be broken through, the low-abundance protein is accurately quantified, the background noise interference is reduced, the detection specificity and accuracy are improved, and the early diagnosis of diseases is assisted.

Fluorescent core-shell particles comprising a core, a first shell disposed on a surface of the core, and a second shell disposed on a surface of the first shell, wherein the core comprises a first polymer selected from a group consisting of a poly(alkyl acrylate), a poly(alkyl methacrylate), and copolymers thereof, the first shell comprises a first polyamine and a second polymer selected from a group consisting of a poly(alkyl acrylate), a poly(alkyl methacrylate), and copolymers thereof, and the second shell comprises a second polyamine and a plurality of carbon dots, wherein at least a portion of the first polyamine is grafted to the second polymer; methods of use and preparation thereof.

The purpose of the present invention is to provide a method for simultaneously detecting a variety of target biomolecules in one implementation using temperature-responsive fluorescent particles. The problem is solved by providing a method for detecting or quantifying biomolecules contained in a biological sample, comprising the following steps (1)-(3): (1) a step in which magnetic particles to which a biomolecule recognition element for capturing is bound, the biological sample, and temperature-responsive fluorescent probe particles are brought into contact with a magnetic substrate; (2) heating the magnetic substrate; and (3) a step for measuring fluorescence emission.

The invention discloses an intelligent obstacle avoidance and navigation system for an unmanned aerial vehicle, and belongs to the technical field of unmanned aerial vehicle path planning, and the system comprises a path obtaining module which releases fluorescent particles according to a first flight safety path based on terrain textures to obtain fluorescent brands, and obtains a group flight path through zero calculation amount optical tracking; the evasion calculation module reconstructs an asymmetric magnetohydrodynamic propulsion field according to the obstacle approaching signal based on the group flight path to obtain obstacle turning and analyzes a three-dimensional air pressure gradient to obtain an evasion path; the signal acquisition module is used for obtaining a control signal by using the quantum entanglement photon pair state verification avoidance path; and the route acquisition module is used for dynamically supplementing the control signal through fluorescence branding to obtain a navigation route, and the problem that in the prior art, flexible obstacle-avoiding flight cannot be performed in a dark and complex environment without a GPS is solved.

The invention provides fluoride fluorescent particles, fluoride fluorescent powder and a preparation method thereof, a light-emitting element and a light-emitting device, and belongs to the technical field of fluorescent powder and light-emitting devices. The chemical formula of the fluoride fluorescent particle is Na2Si (1-X) F6: Mn4 + X, x is greater than 0 and less than 0.1, the fluoride fluorescent particle is of a polyhedral structure, and the polyhedral structure comprises an upper prismatic table, a lower prismatic table and prisms, the upper prismatic table and the lower prismatic table are symmetrically arranged, and the prisms are positioned between the upper prismatic table and the lower prismatic table and are respectively jointed with the lower bottom surface of the upper prismatic table and the lower bottom surface of the lower prismatic table. The fluorescent particle with the polyhedral structure has the characteristics of high emission intensity and high stability, and compared with a rod-shaped structure and an amorphous structure, the fluorescent particle with the polyhedral structure has the advantages that the contact area with water / water vapor after LED packaging is reduced, the dissociation speed is reduced, and the service life of an LED device is prolonged.

The utility model discloses a wavelength conversion assembly and lighting equipment. The wavelength conversion assembly comprises a supporting piece, a first wavelength conversion layer and a second wavelength conversion layer. Wherein the first wavelength conversion layer is arranged on the supporting piece, and the side, away from the supporting piece, of the first wavelength conversion layer is provided with a first area and a second area which are adjacent. The second wavelength conversion layer is arranged in the second area, and the second wavelength conversion layer comprises red fluorescent particles. According to the invention, the second wavelength conversion layer is doped with the red fluorescent particles, so that the specified laser can be converted into red fluorescence under the excitation of the red fluorescent particles. Therefore, when the wavelength conversion assembly is configured in the lighting equipment, the lighting light generated by the lighting equipment can be mixed with the red light component, so that the color rendering index of the lighting light is improved, and the lighting equipment can meet the application scene with the high color rendering index.

The invention discloses a particle printing method, system and device based on fluorescent field and bright field time sequence imaging, and the method comprises the steps: monitoring a micro-fluidic chip sorting region under the condition of continuous illumination of a multi-color fluorescent field, and collecting a fluorescent image of the micro-fluidic chip sorting region in real time; when the target fluorescent particles are recognized in the fluorescent image, bright field illumination is automatically triggered, and a bright field image is collected; carrying out fusion analysis processing on the fluorescence image and the bright field image, and extracting multi-dimensional feature parameters; judging whether the multi-dimensional characteristic parameters meet printing conditions or not according to a preset multi-modal judgment rule; when the multi-dimensional characteristic parameters meet printing conditions, a printing instruction is sent; and in response to the printing instruction, controlling the pressure and motion control unit to drive the printing channel of the micro-fluidic chip to print the target fluorescent particles to the specified hole site of the microwell plate. According to the particle printing technology combining the advantages of fluorescence imaging and bright field imaging, comprehensive feature analysis and high-precision sorting of particles are achieved.

The utility model discloses a multilayer fluorescent film and LED lamp bead, the multilayer fluorescent film is used for packaging LED chips, the multilayer fluorescent film comprises a flexible transparent adhesive layer and a packaging adhesive layer, the packaging adhesive layer is provided with fluorescent particles, the upper surface of the flexible transparent adhesive layer is attached to the lower surface of the packaging adhesive layer, and the upper surface of the flexible transparent adhesive layer is attached to the lower surface of the packaging adhesive layer. And the lower surface of the flexible transparent adhesive layer is attached to the light-emitting surface of the LED chip. According to the technical scheme, the flexible transparent adhesive layer is attached to the LED chip in the multi-layer fluorescent film, so that the multi-layer fluorescent film can be tightly attached to the LED chip, the packaging adhesive layer provided with the fluorescent particles is separated from the LED chip through the flexible transparent adhesive layer, the excitation efficiency of the fluorescent particles becomes high, and meanwhile, the packaging adhesive layer provided with the fluorescent particles can be separated from the LED chip through the flexible transparent adhesive layer. And the cracking probability of the multi-layer fluorescent film is reduced.

The objective is to provide a semiconductor light-emitting element with reduced ripple in FFP (Flexible Printed Fluorescent Particle). The semiconductor light-emitting element includes a GaN substrate, a first nitride semiconductor portion disposed on the substrate and containing n-type impurities, a second nitride semiconductor portion disposed on the first nitride semiconductor portion and containing n-type impurities, and a third nitride semiconductor portion disposed on the second nitride semiconductor portion and containing n-type impurities. The concentration of n-type impurities in the first nitride semiconductor portion is greater than that in the third nitride semiconductor portion, and the concentration of n-type impurities in the second nitride semiconductor portion is greater than that in the third nitride semiconductor portion. The Al composition ratio of the second nitride semiconductor portion is greater than that of the first nitride semiconductor portion, and the Al composition ratio of the third nitride semiconductor portion is greater than that of the first nitride semiconductor portion.

Provided is an ink jet recording method that enables recording of an image with a wide expressible color gamut and high fluorescence intensity. The ink jet recording method comprises a step of recording an image by applying first and second inks to a recording medium by using an ink jet recording apparatus, including a recording head having ejection orifices configured to eject the first and second inks, respectively, such that an area where the first ink is applied and an area where the second ink is applied at least partially overlap each other. The first ink contains an alkaline buffer and a pigment particle dispersed by an effect of an anionic group, the second ink contains a fluorescent particle dispersed by an effect of an anionic group, and a density ρ1 (g / cm3) of the pigment particle is more than a density ρ2 (g / cm3) of the fluorescent particle.

This invention discloses a method and system for evaluating the soothing efficacy of cosmetics based on a deep learning model. The method includes: setting up a control group and a test sample group; after each group is cultured for the same time, zebrafish are fixed with methylcellulose, and side views of the zebrafish in each group are captured under a fluorescence microscope as the original zebrafish images; a binary mask is obtained based on the preprocessed original zebrafish images; the preprocessed original zebrafish images and the corresponding binary masks are used as samples in the training dataset; a semantic segmentation model is trained using the training dataset; the zebrafish images to be detected in each group are preprocessed and input into the trained semantic segmentation model to obtain images containing only the target region in each group, and then the number of fluorescent particles in each group is extracted to evaluate the soothing effect of the cosmetics. This invention combines fluorescence imaging technology and a deep learning model, which can quickly and accurately calculate the number of fluorescent particles in key regions of zebrafish images, thereby realizing the evaluation of the soothing efficacy of cosmetics.