Microchannel speed distribution measuring apparatus and method

A velocity distribution and measurement device technology, which is applied in the direction of fluid velocity measurement, measurement device, velocity/acceleration/impact measurement, etc., can solve the problems of unsatisfactory observation and fluorescent particle observation, and achieve high adjustment accuracy and high spatial resolution , The effect of high optical detection sensitivity

Inactive Publication Date: 2008-02-13
INST OF MECHANICS - CHINESE ACAD OF SCI
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Problems solved by technology

Therefore, the current commercial products cannot observe fluorescent particles with a diameter of less than 1 ...

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  • Microchannel speed distribution measuring apparatus and method

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Embodiment Construction

[0033] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0034] Referring to Fig. 1, make the schematic diagram of the measuring device of the microchannel velocity distribution of the present invention. Among them, microscope 10 adopts Olympus IX71 inverted fluorescence microscope, has white light and UV light dual light source, has 10X, 40X, 60X and 100X objective lens 20, wherein 100X is oil lens, numerical aperture NA=1.35, lens oil refractive index n=1.516, Optical resolution δ = 0.35μm, working distance is 100μm. The reflection device 17 adopts a right-angle reflection prism.

[0035] The optical system 11 adopts commercially available conventional focusing lens and collimating lens products.

[0036] Double-pulse laser 12 adopts NewWave Solo PIV120, Nd:YAG double-pulse laser, emission wavelength λ=532nm, pulse frequency adjustable from 1-15Hz, spot diameter 5mm, pulse width 3-5ns, maximum las...

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Abstract

The invention discloses a measuring device and a measuring method for micro-channel velocity distribution, including a microscope, a double-pulse laser, a photons detector, a synchronous controller and a processor. The method includes the following steps: 1) regulating all instruments to a working state; 2) injecting fluorescent particle solution into micro channel and drive the liquid flowing in the channel; 3) adjusting the vertical position of lens to the clearest imaging position on the bottom surface of the micro channel; 4) the distance between two light spots on the same particle irradiating by the double-pulse laser should be within 20 to 50 pixels; 5) continuously shooting multi-frame images under the irradiation of the double-pulse laser; 6) adjusting the focal plane of lens to a new position and shoot multi-frame images again; 7) repeating the step 6) and shooting images in different positions. The invention has the advantages of high optical sensitivity, high spatial resolution of flow speed detection and high adjustable accuracy of vertical displacement.

Description

technical field [0001] The invention relates to a measuring device and a measuring method for the velocity distribution of a microfluidic channel. Background technique [0002] The main flow characteristics of micro-scale flow: (1) In this characteristic scale range, the ratio of surface area to volume increases to 10 6 m -1 , the heat transfer and mass transfer processes related to the surface have a great influence on the flow; (2) scale reduction makes some gradients in the flow field larger, and the effects of physical parameters related to velocity gradients and temperature gradients will be enhanced: (3 ) The effect of interface force (liquid-solid, liquid-gas) on the flow will be significantly enhanced. In order to gain a deep understanding of these new flow problems, it is particularly important to carry out experimental observations alongside theoretical studies. [0003] Micro-flow is an important content in the research of MEMS (Micro-Electro-Mechanical System,...

Claims

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Application Information

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IPC IPC(8): G01P5/20G01P5/00G01M10/00
Inventor 李战华郑旭王绪伟
Owner INST OF MECHANICS - CHINESE ACAD OF SCI
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