Microchannel speed distribution measuring apparatus and method

A velocity distribution and measurement device technology, which is applied in the direction of fluid velocity measurement, measurement device, velocity/acceleration/impact measurement, etc., can solve the problems of unsatisfactory observation and fluorescent particle observation, and achieve high adjustment accuracy and high spatial resolution , The effect of high optical detection sensitivity

Inactive Publication Date: 2009-09-16
INST OF MECHANICS - CHINESE ACAD OF SCI
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  • Description
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Problems solved by technology

Therefore, the current commercial products cannot observe fluorescent particles with a diameter of less than 1 μm, and cannot meet the observation of micro-pipes and near-wall flow with a cross-sectional size of tens of microns.

Method used

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  • Microchannel speed distribution measuring apparatus and method

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Embodiment Construction

[0033] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0034] refer to figure 1 Schematic diagram of the measuring device for making the microchannel velocity distribution of the present invention. Among them, microscope 10 adopts 0lympus IX71 inverted fluorescence microscope, has dual light sources of self-light and UV light, and has 10X, 40X, 60X and 100X objective lenses 20, wherein 100X is an oil lens, numerical aperture NA=1.35, lens oil refractive index n=1.516 , optical resolution δ = 0.35μm, working distance of 100μm. The reflection device 17 adopts a right-angle reflection prism.

[0035] The optical system 11 adopts commercially available conventional focusing lens and collimating lens products.

[0036] Double-pulse laser 12 adopts NewWave Solo PIV120, Nd:YAG double-pulse laser, emission wavelength λ=532nm, pulse frequency adjustable from 1-15Hz, spot diameter 5mm, pulse width 3-5ns, m...

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Abstract

The invention discloses a measuring device and a measuring method for the velocity distribution of a microfluidic channel. The device includes: a microscope; a double pulse laser, a light quantum detector; a synchronous controller; a processor. The method comprises the following steps: 1) adjusting each instrument to a ready working state; 2) injecting fluorescent particle solution into the micro-channel to drive the liquid flow in the channel; 3) adjusting the vertical position of the objective lens until the bottom surface of the micro-channel is most imaged. Clear position; 4) The distance between two spots of the same particle obtained by double-pulse laser irradiation is 20-50 pixels; 5) Under double-pulse laser irradiation, multiple frames of images are continuously taken; 6) Adjust the focal plane of the objective lens to the new Take multiple frames of images at the position; 7) Repeat step 6) to take images at different positions. The invention has the advantages of high optical detection sensitivity, high spatial resolution of flow field velocity detection and high vertical displacement adjustment precision.

Description

technical field [0001] The invention relates to a measuring device and a measuring method for the velocity distribution of a microfluidic channel. Background technique [0002] The main flow characteristics of micro-scale flow: (1) In this characteristic scale range, the ratio of surface area to volume increases to 10 6 m -1 , the heat transfer and mass transfer processes related to the surface have a great influence on the flow; (2) scale reduction makes some gradients in the flow field larger, and the effects of physical parameters related to velocity gradients and temperature gradients will be enhanced; (3 ) The effect of interface force (liquid-solid, liquid-gas) on the flow will be significantly enhanced. In order to gain a deep understanding of these new flow problems, it is particularly important to carry out experimental observations alongside theoretical studies. [0003] Micro-flow is an important content in the research of MEMS (Micro-Electro-Mechanical System,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01P5/20G01P5/00G01M10/00
Inventor 李战华郑旭王绪伟
Owner INST OF MECHANICS - CHINESE ACAD OF SCI
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