Method of qualitatively analyzing sources of gelatin in hide gelatins

A qualitative analysis, donkey-hide gelatin cake technology, applied in the field of food analysis, can solve the problems of high sensitivity, poor reproducibility, low detection accuracy, etc.

Active Publication Date: 2017-12-29
河北东汝阿胶制药股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the defects of the prior art that the detection sensitivity of the source identification of glue products is low, and the detection accuracy and reproducibility of glue products with low content of animal-derived components are not high, Provide a method for qualitatively analyzing the source of gelatin in donkey-hide gelatin cakes. This method can trace the origin of various animal-derived components in snacks and nutritional products containing different amounts of donkey-hide gelatin. It has good accuracy and high sensitivity.

Method used

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  • Method of qualitatively analyzing sources of gelatin in hide gelatins
  • Method of qualitatively analyzing sources of gelatin in hide gelatins
  • Method of qualitatively analyzing sources of gelatin in hide gelatins

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Experimental program
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Embodiment approach

[0064] According to a preferred embodiment of the present invention, the method for the qualitative analysis of gelatin source in donkey-hide gelatin cake comprises the following steps:

[0065] (1) Extraction and purification of gelatin

[0066] (1-1) Dissolve 10 g of donkey-hide gelatin cake with 50 mL of distilled water to prepare a dispersion of donkey-hide gelatin cake, filter the resulting dispersion with qualitative filter paper and collect the filtrate;

[0067] (1-2) Contact the filtrate collected in step (1-1) with acetone, mix the contacted mixture evenly, place it at -20°C for 1 hour, then centrifuge at 4000g for 15 minutes, collect the centrifuged The precipitate obtained after separation, wherein the volume ratio of the amount of acetone to the filtrate is 4:1;

[0068] (1-3) Redisperse the precipitate collected in step (1-2) in 20mL of distilled water and redissolve, then transfer to a 30000Da ultrafiltration tube for centrifugal ultrafiltration treatment, centri...

Embodiment 1-5 and comparative example 1-10

[0105] A method used to illustrate the qualitative analysis of the origin of gums in donkey hide gelatin, cowhide gelatin, and pig skin gelatin cakes.

[0106] (1) Extraction and purification of gelatin

[0107] (1-1) Dissolve 10 g of donkey-hide gelatin cake with 50 ml of distilled water to obtain the dispersion liquid of donkey-hide gelatin cake, then filter with qualitative filter paper, and collect the filtrate;

[0108] (1-2) Mix the precipitant and the filtrate according to the volume ratio (ml / ml) in Table 2, mix evenly, and then cool and stand for treatment. The cooling and standing conditions are shown in Table 3, and then the precipitate is collected after centrifugation. The centrifugation conditions are as follows: Shown in Table 3;

[0109] (1-3) Redissolve the gelatin precipitate with 20ml of distilled water, transfer it to an ultrafiltration tube after dissolving, and perform ultrafiltration and centrifugation. The conditions of ultrafiltration and centrifugati...

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Abstract

The invention relates to the field of food analysis and discloses a method of qualitatively analyzing sources of gelatin in hide gelatins. Acetone is used herein as a gelatin extracting agent for hide gelatins, specific endoproteinase Lys-C and trypsin are used to decompose polypeptides of different gelatin sources from hide gelatins into characteristic polypeptide fragments, a high-performance liquid chromatograph-linear ion trap and orbit trap high-resolution combined mass spectrograph apparatus is utilized in a single ion detection scanning mode to separate and detect gelatin enzymatic hydrolysates, a mass spectrum obtained is recorded, and mass spectrum analytical software is used to identify characteristic polypeptides in donkey hide gelatin, pig skin gelatin and cattle hide gelatin. The method provided herein can be used to identify donkey hide gelatin, cattle hide gelatin and pig skin gelatin among hide gelatin products, has high detection accuracy, good reproducibility and sensitivity, and can provide basis for relevant departments to monitor the quality of hide gelatin products.

Description

technical field [0001] The invention relates to the field of food analysis, in particular to a method for qualitatively analyzing the source of gelatin in donkey-hide gelatin cake. Background technique [0002] Donkey-hide gelatin is one of the traditional Chinese medicines. It is produced by partially breaking collagen, the main component of donkey skin. It is widely used to supplement red blood cells and hemoglobin. Ejiao, together with ginseng and deer antler, are known as the three most valuable traditional Chinese medicinal materials. In addition, snacks and nutritional products containing donkey-hide gelatin are also very popular in China. However, in recent years, due to the high demand and limited supply of donkey skin, other gelatins, including cowhide gelatin and pig skin gelatin, have been added to donkey-hide gelatin resulting in the adulteration of donkey-hide gelatin. [0003] In the market, more than 95% of gelatin originates from pigs and cattle. In adulter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 涂叶绿沙小梅胡姿姿唐顺博王豪
Owner 河北东汝阿胶制药股份有限公司
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