Detection method for characterizing brittleness of surimi gel
A detection method and technology of the test method, applied in the field of food analysis, can solve the problems of unfavorable quality monitoring of industrialized products, difficult popularization and application, complicated calculation, etc., and achieve the effect of realizing objective measurement, expanding research scope, and simple measurement process.
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Embodiment 1
[0030] (1) The specific preparation steps of different brittle surimi gels are as follows:
[0031] Preparation of surimi gel samples:
[0032] After thawing the frozen surimi at 4°C, adjust its water content to 78%, chop it with a chopping machine for 2 minutes, add 2.5% NaCl and different concentrations of TGase (0U / g protein, 6U / g protein, 12U / g Protein TGase), crushed at 45r / min for 30min, vacuum exhausted, enema sealed, then gelatinized at 40°C for different times (2h, 4h, 6h, 8h), and then gelatinized at 90°C for 0.5h, Cool in cold water, set aside overnight at 4°C, and prepare a series of gradient surimi gel samples with different brittleness.
[0033] (2) Sensory evaluation of the brittleness of surimi gel
[0034] The sensory evaluation of food brittleness is mainly based on the subjective feeling of the product when the sample is chewed and broken by the teeth, and is comprehensively scored from three aspects: force, sound and crushability. The sensory evaluation ...
Embodiment 2
[0041] Using the TA-XT2i / 25 texture analyzer produced by Stable Micro Systems, UK, combined with the corresponding probes, the prepared surimi gel samples with different brittleness were measured. The specific implementation steps are as follows:
[0042] (1) Texture determination of surimi gel brittleness
[0043] The surimi gel samples were tested by TA-XT 2i / 25 physical property tester. The specific test conditions and parameters are as follows:
[0044] Compression breaking test The surimi gel is made into a standard cylinder with a height of 20mm and a diameter of 22mm. Using the P / 36R cylindrical probe, a single compression of the sample causes the sample to undergo a compression test (see figure 1 ); the compression ratio is 80%; the speed before the test is 2mm / s, the speed during the test is 1mm / s, and the speed after the test is 1mm / s; the trigger force is 5g; the same sample is tested 6 times. The stress-strain curve (see figure 2 ) can be fitted by the follow...
Embodiment 3
[0059] In order to further test the application effect of the detection method of the present invention in gel brittleness, make surimi gel samples of different textures again, carry out sensory evaluation and mechanical measurement to its gel brittleness according to the same method respectively, and the mechanical measurement results are based on The characterization model of the present invention calculates a predicted brittleness value. Select the Pr>F value of the f test, the Pr>|t| value of the t test, and the residual mean square value (RMS) to evaluate the practicability of the brittleness detection method of the present invention, and the results are shown in Table 5.
[0060] Table 5 Verification of surimi gel brittleness characterization model
[0061]
[0062] It can be seen from Table 5 that there is no significant difference between the brittleness value of the surimi gel sample predicted by the model and the sensory brittleness value. The sensory brittleness...
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