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Nucleic acid gel fibers and preparing method thereof

A technology of gel fiber and nucleic acid, which is applied in the fields of fiber chemical characteristics, stretch spinning, rayon manufacturing, etc., can solve the problems of lack of internal structure, hindering practical application, poor mechanical properties, etc., and achieves simple preparation process and regular structure. , the effect of excellent mechanical properties

Active Publication Date: 2019-06-21
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This property makes nucleic acids hold great promise as soft materials, but their poor mechanical properties hinder their practical application in fields that require mechanical integrity and tunability.
In addition, nucleic acid-based gel systems are mostly amorphous materials, lacking an ordered internal structure within the gel network, which hinders the study of their anisotropic electrical, optical, magnetic, or mechanical properties.

Method used

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  • Nucleic acid gel fibers and preparing method thereof
  • Nucleic acid gel fibers and preparing method thereof
  • Nucleic acid gel fibers and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 210 μL of 5.4mM 22bp (22 base pairs) DNA solution and 5mL of 50mM didodecyldimethylammonium bromide into a centrifuge tube, shake and stir for 1 minute to form a white suspension. Then put it into a centrifuge, centrifuge at a centrifugal speed of 10000rpm for 30 minutes, remove the supernatant, add deionized water to wash and centrifuge, remove the supernatant after repeated washing three times, and put the centrifuged complex into the liquid Freeze in nitrogen and freeze dry in a lyophilizer for two hours. Then, 100 μL of toluene was added to the freeze-dried sample, and left at room temperature for 30 minutes to obtain a nucleic acid gel, and the nucleic acid gel was drawn to obtain a nucleic acid gel fiber. figure 1 The nucleic acid gel prepared for this example shows that the nucleic acid gel of the present invention has a liquid crystal structure.

Embodiment 2

[0037] Take 210 μL of 5.4mM DNA solution (200 base pairs) and 5mL of 50mM didodecyldimethylammonium bromide into a centrifuge tube, shake and stir for 1 minute to form a white suspension. Then put it into a centrifuge, centrifuge at a centrifugal speed of 10000rpm for 30 minutes, remove the supernatant, add deionized water to wash and centrifuge, remove the supernatant after repeated washing three times, and put the centrifuged complex into the liquid Freeze in nitrogen and freeze dry in a lyophilizer for two hours. Then, 100 μL of toluene was added to the freeze-dried sample, and left at room temperature for 30 minutes to obtain a nucleic acid gel, and the nucleic acid gel was drawn to obtain a nucleic acid gel fiber. figure 1 The nucleic acid gel prepared for this example shows that the nucleic acid gel of the present invention has a liquid crystal structure.

Embodiment 3

[0039] Take 210 μL of 5.4mM DNA solution (1000 base pairs) and 5mL of 50mM didodecyldimethylammonium bromide into a centrifuge tube, shake and stir for 1 minute to form a white suspension. Then put it into a centrifuge, centrifuge at a centrifugal speed of 10000rpm for 30 minutes, remove the supernatant, add deionized water to wash and centrifuge, remove the supernatant after repeated washing three times, and put the centrifuged complex into the liquid Freeze in nitrogen and freeze dry in a lyophilizer for two hours. Then, 100 μL of toluene was added to the freeze-dried sample, and left at room temperature for 30 minutes to obtain a nucleic acid gel, and the nucleic acid gel was drawn to obtain a nucleic acid gel fiber. figure 2 The nucleic acid gel extensibility experiment prepared for this embodiment shows that the nucleic acid liquid crystal gel has a large elongation at break.

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Abstract

The invention relates to the technical field of biological materials, in particular to nucleic acid gel fibers and a preparing method thereof. The preparing method comprises the steps that nucleic acid and a cationic surface active agent are subjected to static reaction in a water phase to obtain a compound, the compound is centrifuged, precipitated, washed, frozen-dried and added into an organicsolvent, and after swelling and drawing, the nucleic acid gel fibers are obtained. The prepared nucleic acid gel fibers have high stretching strength and modulus and excellent extensibility and keep the liquid crystal structure in an organic phase, and the toughness of the nucleic acid gel fibers can rival that of natural spider silk fibers. Meanwhile, the technology of the preparing method is simple, no special equipment is needed, and the cost is low.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a nucleic acid gel fiber and a preparation method thereof. Background technique [0002] Nucleic acid is a large biomolecule usually located in the nucleus, which is responsible for the carrying and transmission of genetic information of organisms, and can be used as a structural unit for the assembly of nanostructures. It has always been one of the most studied biomacromolecules in the field of biomaterial technology. In recent years, due to the mature development of automated solid-phase synthesis, molecular cloning technology and polymerase chain reaction, the study of short-chain nucleic acids and long-chain nucleic acids has become possible. Due to the unique properties of nucleic acids, such as self-recognition ability, sequence programmability and excellent stability. This makes it a hotspot in the field of functionalized biomaterials research. Among them, due to it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F9/00D01F1/10D01D5/12
Inventor 刘凯孟卓君王帆张洪杰
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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