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Circulating tumor cell sorter and kit

A technology of tumor cells and kits, applied in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as cumbersome operations, unrealized capture, staining, and analysis automation

Active Publication Date: 2017-07-11
ZHONGSHAN HOSPITAL FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The system includes a capture and staining device Autoprep, a sample box device MagNest with a magnetic field, and a cell counting device Analyzer. It can be seen that the system does not realize the whole process of automation from sample pretreatment to cell capture, staining, and analysis, and its operation is still relatively cumbersome

Method used

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  • Circulating tumor cell sorter and kit
  • Circulating tumor cell sorter and kit
  • Circulating tumor cell sorter and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Such as figure 1 Shown is a schematic top view of a preferred embodiment of the circulating tumor cell sorting apparatus of the present invention. The sorting apparatus is divided into two main structures with the sliding track in the middle as the boundary. The left side is the centrifugal zone and the right side is In the staining and reading area, the sorter shown in the figure can sort and detect 12 samples at the same time. It can be seen from the figure that the circulating tumor cell sorter includes a centrifugal platform 1, a first reagent storage platform 2-1, a second reagent storage platform 2-2, a first waste collection platform 3-1, and a second waste collection platform 3-2, first transfer part 4-1, second transfer part 4-2, third transfer part 4-3, fourth transfer part 4-4, first transfer head storage platform 5-1, second transfer head Storage platform 5-2, magnetic bar storage platform 6, magnetic bar 7, magnetic bar support frame 8, dyeing rack 9, cleani...

Embodiment 2

[0083] figure 2 It is a schematic top view of another preferred embodiment of the present invention. In the figure, 1 is a centrifugal platform, 2 is a reagent storage platform, 3 is a waste collection platform, 4-1 is a magnetic rod transfer component, and 4-2 is a reagent transfer component. 5 is the transfer head storage platform, 6 is the magnetic bar storage platform, 7 is the magnetic bar, 8 is the magnetic bar support frame, 9 is the washing and dyeing rack, 11 is the linear track, 12 is the counting platform, this embodiment also includes the stirring bar storage Platform 13, the stirring rod can be used for stirring and mixing the solution.

[0084] In this embodiment, the function of the rotating transport component is incorporated into the sliding transport component. The magnetic rod transport component 4-1 and the reagent transport component 4-2 have a common sliding track, which can move in the three-dimensional space of XYZ. The moving surface covers the operating...

Embodiment 3

[0086] This embodiment provides a magnetic rod, and the structure of the magnetic rod 7 is as follows image 3 As shown, it includes a plunger 71, a magnetic core 72, and a magnetic sleeve 73. The magnetic sleeve 73 is sleeved on the outside of the plunger 71 like a pen sleeve, and the magnetic core 72 is wrapped inside, on the magnetic sleeve 73 A capture window 74 is provided. The surface of the magnetic core 72 corresponding to the capture window 74 is a magnetic field emission surface. The magnetic field can radiate outward through the window. No other surface of the magnetic core 72 emits a magnetic field outward, that is, the other surfaces are soft magnetic. The material is wrapped so that the magnetic nano particles in the capture reagent are gathered in the capture window 74. When the magnetic nano particles with the target CTC are captured and collected, only the plunger 71 is drawn out of the magnetic sleeve 73 to directly The cells on the capture window 74 are subjec...

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Abstract

The invention discloses a circulating tumor cell sorter, the sorter is compatible with purposes, CTC negative sorting and positive sorting can be achieved in one instrument, and negative sorting and positive sorting results can be mutually verified and mutually complemented, so that more accurate detection results can be obtained; CTC capture specificity can be increased by positive enrichment, a CTC sample with a higher purity can be provided; a CTC capture range can be expanded by the negative sorting, and more research objects can be provided; the circulating tumor cell sorter has reasonable layout and compact structure; the invention also further provides a kit for the circulating tumor cell sorter, and by combination of the circulating tumor cell sorter and the kit, a sorting process can be more smooth and efficient by a best operation process.

Description

Technical field [0001] The invention relates to a circulating tumor cell sorter and its kit. Background technique [0002] Cancer cells separated from the primary tumor site are called "circulating tumor cells (CTC)". According to literature reports, CTC can be released into the blood at the early stage of malignant tumor formation, and CTC is released into the blood circulation from the primary tumor, which is a key step for the recurrence and metastasis of malignant tumors. In the past two decades, CTC has become an emerging “biomarker” for detecting early cancer metastasis, predicting patient prognosis, and detecting disease progression and cancer treatment effects. However, because CTC contains a large number of blood cells (10 9 Cells / mL) blood content is extremely low (several to several hundred per milliliter), so the sorting of CTCs is technically difficult. The rapid and efficient separation of CTCs from peripheral blood is a follow-up CTC cell count, and the premise o...

Claims

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Application Information

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IPC IPC(8): C12M1/00
Inventor 樊嘉周俭杨欣荣孙云帆温冬彭海翔
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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