Chip interchangeable microflow control chip proteolysis reactor

Abstract

The invention relates to a protein enzyme-hydrolysis reactor which can change microcurrent control chip and the preparing method in the microcurrent control chip field. The reactor comprises a microcurrent control chip with two-tip single channel and a glass fiber(enzyme centre)fixed proteinase, wherein the enzyme centre penetrates the microcurrent channel of the chip and goes out from two tips, the enzyme centre modifies chitose film on the surface with dip-coating method by the glass fiber and is made by fixing the proteinase to the surface of the glass fiber with adsorption, embedding or covalence bond technique, the protein enzyme-hydrolysis reactor which can change microcurrent control chip is acquired by inserting the glass fiber enzyme centre in the micro channel of microcurrent control chip before using. The glass fiber enzyme centre has the inexpensive price, which can change according to the requirements, improves the using efficient of the chip and the enzyme, and reduces the measuring cost of enzyme method. The reactor has the good application prospect in the clinical diagnosis, the environmental monitoring, the life science research and the food analysis field.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a replaceable core-type microfluidic chip proteolysis reactor and its preparation technology. Background technique [0002] Since Manz and Widmer[1] of Ciba-Geigy in Switzerland first proposed a miniature total analysis system in 1990, the microfluidic chip has attracted domestic attention due to its advantages of high efficiency, rapidity, less reagent consumption, low consumption and high integration. Based on the rapid development of its methodological research, microfluidic chips have shown good application prospects in the fields of biomedical research, clinical diagnosis, drug analysis, environmental monitoring, forensic medicine and military[2-4] . [0003] The size of the microfluidic channel of the microfluidic chip is at the micron level, which is an ideal operation and analysis platform for small-volume samples from nanoliters to microliters, an...

AI Technical Summary

Problems solved by technology

The problem is that once the enzyme is inactivated, the entire chip will be scrapped. At present, the price of the chip is still tens or even hundreds of yuan per piece, which limits the widespread use of microfluidic chip enzyme reactors.

In addition, scrapped chips will have an impact on the environment after being discarded.

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