Chip interchangeable microflow control chip proteolysis reactor

A technology of microfluidic chip and proteolysis, which is applied in the field of replaceable microfluidic chip proteolysis reactor and its preparation, which can solve the problems of scrapping chips, limiting the widespread use of microfluidic chip enzyme reactors, and environmental impact. , to achieve the effect of improving use efficiency, good application prospects, and saving resources

Inactive Publication Date: 2008-01-02
FUDAN UNIV
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Problems solved by technology

The problem is that once the enzyme is inactivated, the entire chip will be scrapped. At present, the price of the chip is still tens or even hundreds of yuan per piece, which limits the widespread use of microfluidic chip enzyme reactors.
In addition, scrapped chips will have an impact on the environment after being discarded.

Method used

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  • Chip interchangeable microflow control chip proteolysis reactor
  • Chip interchangeable microflow control chip proteolysis reactor

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Embodiment Construction

[0028] Further describe the present invention below by embodiment and accompanying drawing:

[0029]1. Preparation of replaceable core microfluidic chip enzyme reactor by layer-by-layer self-assembly method and its application

[0030] Computer-aided design software is used to design the chip structure, which is composed of a single microfluidic channel. A high-resolution (such as 3600dpi) laser phototypesetting system is used to print a mask on a transparent film. The microchannel part is black lines with a width of 60 microns, and other parts is transparent. A layer of positive photoresist (Shipley S1813 Photoresist, Shipley, Marlborough, MA, USA), spin-coating conditions were 3000 rpm for 60 seconds. Then bake at 110°C for 60 seconds to improve the adhesion of the photoresist and remove the residual solvent (baking before exposure), and then cover the mask (including the designed microfluidic structure), using Karl Suss MA6 / BA6 photolithography After 40 seconds of contac...

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Abstract

The invention relates to a protein enzyme-hydrolysis reactor which can change microcurrent control chip and the preparing method in the microcurrent control chip field. The reactor comprises a microcurrent control chip with two-tip single channel and a glass fiber(enzyme centre)fixed proteinase, wherein the enzyme centre penetrates the microcurrent channel of the chip and goes out from two tips, the enzyme centre modifies chitose film on the surface with dip-coating method by the glass fiber and is made by fixing the proteinase to the surface of the glass fiber with adsorption, embedding or covalence bond technique, the protein enzyme-hydrolysis reactor which can change microcurrent control chip is acquired by inserting the glass fiber enzyme centre in the micro channel of microcurrent control chip before using. The glass fiber enzyme centre has the inexpensive price, which can change according to the requirements, improves the using efficient of the chip and the enzyme, and reduces the measuring cost of enzyme method. The reactor has the good application prospect in the clinical diagnosis, the environmental monitoring, the life science research and the food analysis field.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a replaceable core-type microfluidic chip proteolysis reactor and its preparation technology. Background technique [0002] Since Manz and Widmer[1] of Ciba-Geigy in Switzerland first proposed a miniature total analysis system in 1990, the microfluidic chip has attracted domestic attention due to its advantages of high efficiency, rapidity, less reagent consumption, low consumption and high integration. Based on the rapid development of its methodological research, microfluidic chips have shown good application prospects in the fields of biomedical research, clinical diagnosis, drug analysis, environmental monitoring, forensic medicine and military[2-4] . [0003] The size of the microfluidic channel of the microfluidic chip is at the micron level, which is an ideal operation and analysis platform for small-volume samples from nanoliters to microliters, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
Inventor 陈刚樊慧芝储艳秋陈挚杨芃原
Owner FUDAN UNIV
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