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375 results about "Peptide breakdown" patented technology

Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years.

Compositions and methods for enhancing structural and functional nervous system reorganization and recovery

The present invention provides methods and compositions for enhancing recovery in a subject suffering from damage to the nervous system. In particular, the invention includes a method for promoting recovery and / or reorganization in the nervous system of a subject in need of enhancement of recovery and / or reorganization of the nervous system as a result of ischemic, hemorrhagic, neoplastic, degenerative, or traumatic damage by focally administering a composition comprising a proteolysis-enhancing agent such as tissue plasminogen activator (tPA), plasmin, or a PAI inhibitor to the nervous system of the subject. In some embodiments an additional active agent is also administered. The composition can be delivered using a variety of techniques including injection, via infusion pump, from an implantable microchip, or using a polymeric delivery vehicle. The composition can be administered, for example, to one or more subdivisions or areas of the brain, the spinal cord, or to one or more nerves or nerve tracts innervating diverse regions of the body. The invention also includes a drug delivery device for implantation into the nervous system to promote nervous system reorganization and / or recovery following ischemic, hemorrhagic, neoplastic, traumatic or degenerative damage, the drug delivery device comprising a biocompatible polymer and a proteolysis-enhancing agent such as tissue plasminogen activator (tPA), plasmin, or a PAI inhibitor, wherein the proteolysis-enhancing agent is released from the polymer in an amount effective to promote structural reorganization of the nervous system. In some embodiments the biocompatible polymer is a hydrogel.
Owner:THE BRIGHAM & WOMEN S HOSPITAL INC +1

Methods and compositions for evaluating breast cancer prognosis

Methods and compositions for evaluating the prognosis of a breast cancer patient, particularly an early-stage breast cancer patient, are provided. The methods of the invention comprise detecting expression of at least one, more particularly at least two, biomarker(s) in a body sample, wherein overexpression of the biomarker or a combination of biomarkers is indicative of breast cancer prognosis. In some embodiments, the body sample is a breast tissue sample, particularly a primary breast tumor sample. The biomarkers of the invention are proteins and / or genes whose overexpression is indicative of either a good or bad cancer prognosis. Biomarkers of interest include proteins and genes involved in cell cycle regulation, DNA replication, transcription, signal transduction, cell proliferation, invasion, proteolysis, or metastasis. In some aspects of the invention, overexpression of a biomarker of interest is detected at the protein level using biomarker-specific antibodies or at the nucleic acid level using nucleic acid hybridization techniques.
Owner:TRIPATH IMAGING INC

Multiple Gene Expression including sORF Constructs and Methods with Polyproteins, Pro-Proteins, and Proteolysis

Disclosed are useful constructs and methods for the expression of proteins using primary translation products that are processed within a recombinant host cell. Constructs comprising a single open reading frame (sORF) are described for protein expression including expression of multiple polypeptides. A primary translation product (a pro-protein or a polyprotein) contains polypeptides such as inteins or hedgehog family auto-processing domains, or variants thereof, inserted in frame between multiple protein subunits of interest. The primary product can also contain cleavage sequences such as other proteolytic cleavage or protease recognition sites, or signal peptides which contain recognition sequences for signal peptidases, separating at least two of the multiple protein subunits. The sequences of the inserted auto-processing polypeptides or cleavage sites can be manipulated to enhance the efficiency of expression of the separate multiple protein subunits. Also disclosed are independent aspects of conducting efficient expression, secretion, and / or multimeric assembly of proteins such as immunoglobulins. Where the polyprotein contains immunoglobulin heavy and light chain segments or fragments capable of antigen recognition, in an embodiment a selectable stoichiometric ratio is at least two copies of a light chain segment per heavy chain segment, with the result that the production of properly folded and assembled functional antibody is made. Modified signal peptides, including such from immunoglobulin light chains, are described.
Owner:ABBOTT LAB INC

Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants

The present invention relates to protease resistant mutants of human growth hormone, which contain newly introduced proteolysis resistant mutations and N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.
Owner:NOVO NORDISK AS +1

Tumor activated prodrugs

The instant invention provides compositions comprising a prodrug, the prodrug comprising a therapeutically active drug; and a peptide selected from the group consisting of the sequences: Ser-Ser-Lys-Tyr-Gln (SEQ ID NO:1);Gly-Lys-Ser-Gln-Tyr-Gln (SEQ ID NO:2); and Gly-Ser-Ala-Lys-Tyr-Gln (SEQ ID NO:3) wherein the peptide is linked to the therapeutically active drug to inhibit the therapeutic activity of the drug, and wherein the therapeutically active drug is cleaved from the peptide upon proteolysis by an enzyme having a proteolytic activity of prostate specific antigen (PSA). The invention further provides methods of making and using the claimed compositions.
Owner:INSPYR THERAPEUTICS INC

Protein hydrolysates produced with the use of cod proteases

The invention relates to a method for enzymatically obtaining protein hydrolysates for human consumption, animal feed and cosmetics. The process involves the use of a proteolytic composition derived from fish, such as Cod (Gadus morhua), to obtain hydrolysates which have a non-bitter taste and retain the flavor and aroma of the protein-containing material which is hydrolyzed: e.g. when hydrolyzing protein-containing material from marine organisms or parts thereof, such as fish, shrimp, lobster or other seafood according to the invention, a protein hydrolysate is produced that has a characteristic natural flavor of the organism Also provided are food products comprising the hydrolysates of the invention, such as soup, sauce, cheese, HVP, meat extract and flavoring agent, broth, paté, mousse, frying dough, orly dough, and pastries.
Owner:NORDUR EHF

Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants

The present invention relates to protease resistant mutants of human growth hormone, which contain newly introduced proteolysis resistant mutations and N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.
Owner:NOVO NORDISK AS

Method for extracting and preparing protein powder and enzymolysis product thereof from almond meal

The invention discloses a method for continuously extracting and preparing concentrated protein, separated protein and proteolysis products from almond meal. The method comprises the following steps of: degreasing and debitterizing residual oil meal obtained after almond oil is mechanically and coldly pressed, and then extracting with a low-concentration NaCl phosphate buffer solution to obtain the concentrated protein; preparing almond separated protein from the concentrated protein solution by applying an ultrafiltration membrane technology; preparing a proteolysis-almond polypeptide mixed solution from the separated protein solution by applying an enzymolysis technology; and ultrafiltering the polypeptide mixed solution by applying a membrane technology to obtain multifunctional active almond peptide with different class level molecular weights in sequence. The method has the advantages of simple process, strong operability and easiness in control of extracting conditions and can finish the extraction and preparation of the almond concentrated protein, the separated protein and the proteolysis products in sequence; and the obtained products have high purity and wide application and can be directly used as food additives, nutrition reinforcers, health care products, medicaments, and the like needed by the protein foods and different processing foods.
Owner:NORTHWEST A & F UNIV

Genetically modified cells and methods for expressing recombinant heparanase and methods of purifying same

Bacterial, yeast and animal cells and methods for overexpressing recombinant heparanase in cellular systems, methods of purifying recombinant heparanase therefrom and modified heparanase species which serve as precursors for generating highly active heparanase by proteolysis.
Owner:INSIGHT BIOPHARMLS

Plant lipid cream and production method thereof

The invention discloses a plant lipid cream and production method thereof. The plant lipid cream is prepared by the steps that proteolytic enzyme is utilized to restrict hydrolyzed soy separation, soas to obtain soy proteolysis solution; the soy proteolysis solution is mixed with partially hydrogenated plant oil, sucrose, starch syrup, sodium alginate tech grade, carrageenan, microcrystalline cellulose, phosphate, edible emulsifier; and then filtering, aqueous phase material preparation, oil phase material preparation, mixing, blending, secondary homogeneity, aging, filling and packing are carried out sequentially. The invention modifies enzymolysis of isolated soy protein, dissolubility, dispersity and emulsibility are obviously improved, and the functional characteristic is close to casein, thus substituting application of nutrose in plant lipid cream; plant raw materials are adopted, raw material cost is reduced, thus having positive significance on nutrition and health care function; the product has favourable mouthfeel and flavour; and the production method of the invention is based on plant lipid cream similarity processing technology, utilizes biological enzyme technology and has wide applicability.
Owner:GUANGDONG IND TECHN COLLEGE

Processing method for cheese suitable for taste of Chinese people

The invention relates to the technical field of dairy product processing, in particular to a processing method for natural cheese suitable for the taste of Chinese people. In the method, fresh milk is pasteurized after being filtered, purified and standardized, and is then cooled to the curding temperature, compound starter is first added for preacidification, the acidity is then regulated, calcium chloride and compound rennin are then added, curds are formed by heat preservation and then cut into curd blocks, whey is discharged after the curd blocks are stirred and heated and the temperature is preserved, and the curd blocks are taken out, added with salt and Chinese ham powder, molded, vacuumized and fermented to ripen under the constant temperature. The curding speed is high, the period of fermentation is short, the production cost is low, proteolysis and lipolysis are moderate, the bitterness is light, and since the tastes of the cheese, the acidophilus milk and the Chinese ham are mixed together, the taste of the produced natural cheese is more suitable for the taste of Chinese people.
Owner:ZHEJIANG UNIV +1

Meaty paste essence and preparation method thereof

The invention discloses meaty paste essence and a preparation method thereof. The meaty paste essence comprises the following components in part by weight: 10 to 30 parts of L-cysteine, 15 to 20 parts of vitamin B, 20 to 40 parts of glucose, 10 to 20 parts of L-leucine, 5 to 10 parts of DL-methionine, 2 to 8 parts of alanine, 50 to 150 parts of yeast fine paste, 200 to 300 parts of bean pulp zymolyte, 2 to 5 parts of disodium inosinate and disodium guanylate (I+G), 30 to 60 parts of soy sauce, 50 to 80 parts of animal fat, 50 to 80 parts of salt, 50 to 100 parts of white granulated sugar, 1 to 3 parts of xanthan gum, 50 to 100 parts of sodium glutamate, 30 to 60 parts of corn starch and 10 to 30 parts of meaty flavoring base. The meaty paste essence prepared by combining the Maillard reaction technology and the bean pulp proteolysis technology has good mouthfeel, high environment-friendly indexes, stable quality, low cost and high safety performance.
Owner:TIANNING FLAVOR JIANGSU

Prolyl endopeptidase mediated destruction of T cell epitopes in whole gluten

Celiac Sprue and / or dermatitis herpetiformis are treated by interfering with HLA binding of immunogenic gluten peptides. The antigenicity of gluten oligopeptides and the ill effects caused by an immune response thereto are decreased by administration of an HLA-binding peptide inhibitor. Such inhibitors are analogs of immunogenic gluten peptides and (i) retain the ability to bind tightly to HLA molecules; (ii) retain the proteolytic stability of these peptides; but (iii) are unable to activate disease-specific T cells.
Owner:UNIVERSITY OF OSLO +1

Preparation method of fermented rice sticks

ActiveCN101623065APlay the effect of debranching and straighteningShorten fermentation timeFood preparationAmylaseProteinase activity
The invention relates to a preparation method of fermented rice sticks, which belongs to the field of food fermentation and comprises a pulp making procedure and a pulp steaming procedure. The preparation method of fermented rice sticks is characterized in that 1-1000ml of debranching amylase for per 100kg of rice is added into rice pulp with the weight percent of 5 to 50% before the pulp steaming procedure and after the pulp making procedure, the pH value is regulated to 4.0-5.0, and enzymolysis is carried out for 30-360 min under the temperature of 30-60 DEG C; 1-50 g of protease with proteolysis peptide bonds for per 100kg of rice is added into the rice pulp, and enzymolysis is carried out for 60-360 min under the temperature of 30-60DEG C and the pH value of 4.0-8.0; then, 0.2 ml / kg of zymophyte is added to the pulp and fermented for 360-3600 min under the temperature of 25-30DEG C and the pH value of 5.0-6.0. In the invention, the debranching amylase is utilized to hydrolyze Alpha-D-1, 6 glycoside bonds as the branched chains in starch and dextrine to generate straight-chain compound sugar containing Alpha-D-1, 4 glycoside bonds so as to obtain the effect on debranching and adding straight chains; the protease with proteolysis peptide bonds can be used for hydrolyzing molecular protein and amino acid to provide sufficient amino acid for following mixed fermentation, shorten fermentation time, and improve the quality and the taste of products.
Owner:江西蓓蕾食品有限公司

Immunological assay and antibodies for Anti-Mullerian Hormone

The present invention discloses compositions and methods to measure an amount of Anti-Mullerian Hormone (AMH) in a sample, including a mammalian sample such as a primate, rodent, equine, or bovine sample. The compositions and methods herein also provide antibodies that bind to epitopes on AMH that are stable to proteolysis of AMH.
Owner:OXFORD BROOKES UNIVERSITY +1

Method for detecting 18 varieties of protein hydrolytic amino acids in milk powder through high-performance liquid chromatographic method

The invention belongs to the technical field of amino acid detection, in particular relates to a method for detecting 18 varieties of protein hydrolytic amino acids in milk powder through a high-performance liquid chromatographic method, solving the problems that the traditional amino acid determination method has high cost and poor universality and the qualitative and quantitative analysis of the 18 varieties of standard amino acids after proteolysis cannot be quickly and accurately performed through a high-performance liquid chromatograph at present. The method comprises the steps of: pre-treating the milk powder through three different hydrolysis methods; performing pre-column derivatization of a hydrolyzed milk powder smaple through a CNBF (4-chloro-3,5-dinitrobenzotrifluoride) derivatizing agent; optimizing derivatization conditions and chromatographic fractionation detection conditions by adopting the HPLC (High-performance Liquid Chromatography) and a universal C18 (250mm*4.6mm, 5mu m) liquid chromatography column; and separating and determining all 18 varieties of proteolyzed amino acids in the milk powder. The method has the advantages of stronger universality and no need of the precious amino acid analyzer and / or the precious amino acid determination reagent kit, thereby being suitable for more common laboratories.
Owner:山西出入境检验检疫局检验检疫技术中心

Complete chemical and enzymatic treatment of phosphorylated and glycosylated proteins on protein chip arrays

A simple and quick protocol for chemical treatment, enzymatic or chemical digestion, and subsequent identification of proteins on protein chip arrays is disclosed, together with kits therefor. The chemical treatment comprises denaturation, reduction and alkylation while enzymatic digestion encompasses deglycosylation, dephosphorylation, and digestion by various proteases. Digestion is also accomplished by various chemicals that are known to induce proteolysis. All reactions are carried out sequentially on chip. Subsequent peptide mass fingerprinting or product ion searches allow the identification of specific peptides which can be correlated to proteins. The method of the present invention can be applied to the analysis of biological samples such as urine and plasma to identify biomarkers in diseased states. The methods of the present invention allow complete on chip treatment, which can be used for rapid protein identification and structural characterization of heavily posttranslationally modified proteins.
Owner:GIBBS BERNARD F

GHRH analogues

The present invention relates to growth hormone-releasing hormone (GHRH) analogues. More particularly, the invention relates to synthetic GHRH analogues of amino acids or more, exhibiting concomitantly an increased resistance to proteolysis and high binding affinity to human GHRH receptor in in vitro studies, in comparison with human native GHRH (1-29)NH2. The present invention also relates to a pharmaceutical composition comprising any one of said GHRH analogues and to the use of these analogues for specific stimulation of in vivo GH release as well as preparation of a drug in the treatment of GH deficiency-related conditions. The present invention also provides for a method for initiating GHRH-induced biological actions in a mammal.
Owner:CENT HOSPITALER DE LUNIV DE MONTREAL CHUM

Method for recovering coin protein sugar dregs and preparing protein nitrogen sources and nitrogen-containing syrup

ActiveCN101787382AIncrease content concentrationEasy to useFermentationProtein nitrogenWastewater
The invention discloses a method for recovering coin protein sugar dregs and preparing protein nitrogen sources and nitrogen-containing syrup in the production of enzymatic corn starch sugar, comprising the steps of size mixing, liquefying, saccharifying, standing separation, centrifugal separation, proteolysis, concentration, drying, compounding, and the like. In the invention, protein is condensed under the heat flash of liquefying, the protein sugar dregs concentrate and float upwards in saccharifying, the sugar dregs are recovered through the steps of standing separation and centrifugal separation, the sugar dregs are dried into protein, or the sugar dregs are hydrolyzed to prepare protein nitrogen sources, and the nitrogen sources can be compounded with syrup to form nitrogen-containing starch syrup for fermenting. Clarified saccharification liquid after centrifugal separation is decolored, filtered, refined and concentrated, and starch syrup products are obtained. The majority of lentous sugar dregs in the saccharification liquid are removed before the treatment of decoloring and filtering, and the decoloring and filtering performance of the material is better. The use level of active carbon, and the like can be properly reduced, the production capability of filtering equipment is improved, and the frequency of the loading and unloading of filters as well as the consumption of washing waste water and various kinds of loss are greatly reduced.
Owner:广州双桥(重庆)有限公司

Novel regulatory mechanisms of NF-kappaB

InactiveUS20050147608A1Inhibiting degredation of NF-kBIncreasing nuclear accumulation and protein stabilityHydrolasesAntipyreticIsomerizationProteolysis
The instant invention pertains to the discovery of two novel regulatory mechanisms of NF-kB. The instant invention demonstrates that NF-kB is regulated by Pin1-catalyzed prolyl isomerization and ubiquitin-mediated proteolysis of p65. Accordingly, the instant invention provides methods for regulating NF-kB, and diseases and disorders associated with NF-kB. Further, the invention provides compositions capable of modulating the activity or expression of NF-kB, Pin1, and / or the proteolysis of p65.
Owner:BETH ISRAEL DEACONESS MEDICAL CENT INC

Lactobacillus plantarum CW006 with antihypertensive function

This invention discloses a new Lactobacillus plantarum strain CW006(conservation number CCTCC M 206032) with an antihypertensive effect, and the hygienic and medical agents related, belonging to the micro-biological additives technological field. This Lactobacillus plantarum strain CW006 has a proteolysis activity and can acquire the antihypertensive effect by producing angiotensin-converting enzyme inhibitor via fermentation of raw milk. With these characteristics, it can be used as starting strain in the dairy industry, and applied in various health care products and medical agents as theraputic additives.
Owner:JIANGSU WECARE BIOTECHNOLOGY CO LTD

Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy

Botulinum neurotoxins, the most potent of all toxins, induce lethal neuromuscular paralysis by inhibiting exocytosis at the neuromuscular junction. The light chains (LC) of these dichain neurotoxins are a new class of zinc-endopeptidases that specifically cleave the synaptosomal proteins, SNAP-25, VAMP, or syntaxin at discrete sites. The present invention relates to the construction, expression, purification, and use of synthetic or recombinant botulinum neutoroxin genes. For example, a synthetic gene for the LC of the botulinum neurotoxin serotype A (BoNT / A) was constructed and overexpressed in Escherichia coli. The gene product was purified from inclusion bodies. The methods of the invention can provide 1.1 g of the LC per liter of culture. The LC product was stable in solution at 4° C. for at least 6 months. This rBoNT / A LC was proteolytically active, specifically cleaving the Glu-Arg bond in a 17-residue synthetic peptide of SNAP-25, the reported cleavage site of BoNT / A. Its calculated catalytic efficiency kcat / Km was higher than that reported for the native BoNT / A dichain. Treating the rBoNT / A LC with mercuric compounds completely abolished its activity, most probably by modifying the cysteine-164 residue located in the vicinity of the active site. About 70% activity of the LC was restored by adding Zn2+ to a Zn2+-free, apo-LC preparation. The LC was nontoxic to mice and failed to elicit neutralizing epitope(s) when the animals were vaccinated with this protein. In addition, injecting rBoNT / A LC into sea urchin eggs inhibited exocytosis-dependent plasma membrane resealing.
Owner:UNITED STATES OF AMERICA THE AS REPRESENTED BY THE SEC OF THE ARMY

Assay for ubiquitin mediated proteolysis

The present invention provides methods and compositions useful in the screening for agents that modulate activity of a deubiquitinating enzyme. In one embodiment, the invention relates to methods for measuring a deubiquitination activity comprising combining a deubiquitinating enzyme (DUB) with a substrate comprising a ubiquitin moiety and a fluorescently labeled compound under conditions allowing for deubiquitinating activity and measuring an altered fluorescence polarization and / or fluorescence lifetime of the released fluorescently labeled compound The invention also relates to a substrate library comprising peptides of the formula (Z)a(X)mK(X)n(Z)b useful e.g. for the determination of the substrate specificity of a deubiquitinating enzyme.
Owner:FILIPUZZI IREOS +2

High-sensitivity proteolysis assay

The present invention includes a highly sensitive method for detecting the presence of proteases in a sample which are present at very low levels.
Owner:MERCK SHARP & DOHME CORP
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