Method for extracting and preparing protein powder and enzymolysis product thereof from almond meal

Abstract

The invention discloses a method for continuously extracting and preparing concentrated protein, separated protein and proteolysis products from almond meal. The method comprises the following steps of: degreasing and debitterizing residual oil meal obtained after almond oil is mechanically and coldly pressed, and then extracting with a low-concentration NaCl phosphate buffer solution to obtain the concentrated protein; preparing almond separated protein from the concentrated protein solution by applying an ultrafiltration membrane technology; preparing a proteolysis-almond polypeptide mixed solution from the separated protein solution by applying an enzymolysis technology; and ultrafiltering the polypeptide mixed solution by applying a membrane technology to obtain multifunctional active almond peptide with different class level molecular weights in sequence. The method has the advantages of simple process, strong operability and easiness in control of extracting conditions and can finish the extraction and preparation of the almond concentrated protein, the separated protein and the proteolysis products in sequence; and the obtained products have high purity and wide application and can be directly used as food additives, nutrition reinforcers, health care products, medicaments, and the like needed by the protein foods and different processing foods.

Description

technical field

[0001] The invention relates to almond deep processing technology, in particular to a method for continuously extracting and preparing concentrated protein, isolated protein and proteolysis products from almond dregs. Background technique

[0002] The protein content in almond meal after almond oil extraction reaches about 50%. It contains 18 kinds of amino acids needed by the human body and 8 kinds of essential amino acids for the human body. The proportion of amino acids is reasonable and close to the international reference model. Therefore, almond protein is a vegetable protein with high edible value. At present, plant protein products in the world are mainly divided into defatted protein (purity <60%), concentrated protein (purity >70%) and protein isolate (purity >90%), which have different functional properties and are added to food as functional ingredients. middle. According to the different requirements for added protein content and funct...