3 results about "Prostate-specific antigen" patented technology

The application discloses an electrochemical aptamer sensor and a preparation method and application thereof, and a prostate specific antigen detection method, and successfully develops a reliable electrochemical biological aptamer sensor for super-sensitivity detection of PSA. An aptamer is used as a biological recognition element to specifically recognize PSA. Nano-porous gold (NP-Gold) with high specific surface area and excellent conductivity is used as a support material. With the help of double-stranded specific nuclease (DSN), noise signals are reduced, the proposed aptamer sensor shows excellent performance in determination of PSA in clinical serum samples, has high sensitivity, strong anti-interference ability and reliable stability. These unique advantages make the aptamer sensor a potential choice for detection of PSA.

The invention belongs to the field of biological medicine, and provides an active compound for treating prostatic cancer and a preparation method thereof. A-B-C-D-P five-module linear connection design is adopted, wherein the A module is a prostate specific membrane antigen targeting binding unit, the B module is a prostate specific antigen enzyme digestion recognition sequence HSSKLQ, the C module is a cathepsin B-responsive Val-Cit-p-aminobenzyl self-destruction unit, the D module is a reduction-responsive disulfide bond connecting arm, and the P is an anti-tumor pharmacodynamic fragment. A coupling reaction is accurately controlled through a continuous flow microchannel technology, controllable self-assembly of the 20-60-nanometer nano aggregate is achieved, the average hydration diameter dispersion coefficient is not larger than 0.20, and the nano aggregate stably exists in plasma and selectively releases drugs in a tumor microenvironment. Multiple contradictions among prodrug system process manufacturability, intracellular response release and stable dispersion of the preparation are solved, and wide clinical application value is achieved.

The invention discloses a separated antibody or antigen binding fragment specifically bound with PSA, the antibody has high detection sensitivity to PSA, the affinity can reach 2.9 * 10 <-9 > M, and a variable region sequence of the antibody is further obtained through sequencing. The antibody can be used for preparing a detection reagent of a prostate specific antigen, is further used for detecting and diagnosing prostate cancer, and has a good application prospect.