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Preparation method and application of prostate tumor marker immunosensor

An immunosensor and prostate tumor technology, applied in the field of preparation of prostate tumor marker immunosensors, can solve the problems of poor popularity, human harm, long detection time, etc., and achieve the effects of simple operation, improved sensitivity, and overcoming the complexity of instruments and equipment.

Inactive Publication Date: 2014-03-26
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies generally require complex monitoring instruments, the detection time is long, the popularity is poor, and some reagents are radioactive, which is harmful to the human body

Method used

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  • Preparation method and application of prostate tumor marker immunosensor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, adopt the following steps:

[0025] Step 1 Silver hybridized NH 2 - The preparation of the MCM-48 mesoporous material adopts the following procedures: 1) Add 1.0g of tetraethyl orthosilicate into the Erlenmeyer flask, add 62mL of ultrapure water, stir for 10min, add 0.5g of potassium hydroxide, and then Add 0.65 g of cetyltrimethylammonium bromide at one time, stir for 30 minutes, then transfer to an autoclave, and heat crystallization at 120°C for 62 hours. The resulting white powder is washed thoroughly with ultrapure water and ethanol in turn, and dried at room temperature. Then in a muffle furnace, the temperature was programmed to rise to 550°C at a rate of 1°C / min, and kept for 6 hours to obtain MCM-48; 2) Take 1.0g of MCM-48 and 1mL of 3-aminopropyltriethoxysilane to dissolve In 80mL of anhydrous toluene, reflux at 70°C for 1.5h, centrifuge and dry at 110°C for 1h to obtain powdered NH2-MCM-48; 3) Add 48mL ultrapure water to the Erlenmeyer flask, a...

Embodiment 2

[0029] Embodiment 2, adopt the following steps:

[0030] Step 1 Silver hybridized NH 2 - The preparation of the MCM-48 mesoporous material, the specific steps are as follows: 1) Add 1.2g tetraethyl orthosilicate into the Erlenmeyer flask, add 80mL ultrapure water, stir for 10min, add 1.0g potassium hydroxide, and then Add 1.0 g of cetyltrimethylammonium bromide at one time, stir for 35 minutes, transfer to an autoclave, and heat crystallize at 120° C. for 67 hours. The white powder obtained is washed thoroughly with ultrapure water and ethanol in turn, and dried at room temperature. Then in the muffle furnace, the temperature was programmed to rise to 550°C at a rate of 1°C / min, and kept for 7 hours to obtain MCM-48; 2) Take 1.0g of MCM-48 and 1mL of 3-aminopropyltriethoxysilane to dissolve In 85mL of anhydrous toluene, reflux at 70°C for 2h, centrifuge and dry at 110°C for 1.5h to obtain powdered NH 2 -LMCM-48; 3) Add 48mL ultrapure water to the Erlenmeyer flask, add 1mL si...

Embodiment 3

[0034] Embodiment 3, adopt the following steps:

[0035] Step 1 Silver hybridized NH 2 - The preparation of the MCM-48 mesoporous material, specifically the following procedures: 1) Add 1.5g tetraethyl orthosilicate into the Erlenmeyer flask, add 95mL ultrapure water, stir for 10min, add 1.0g potassium hydroxide, and then Add 1.5 g of hexadecanetrimethylammonium bromide at one time, stir for 40 minutes, transfer to an autoclave, and heat crystallize at 120° C. for 72 hours. The resulting white powder is washed thoroughly with ultrapure water and ethanol in turn, and dried at room temperature. Then in the muffle furnace, the temperature was programmed to rise to 550°C at a rate of 1°C / min, and kept for 8 hours to obtain MCM-48; 2) Take 1.0g of MCM-48 and 1mL of 3-aminopropyltriethoxysilane to dissolve In 90mL of anhydrous toluene, reflux at 70°C for 2h, centrifuge and dry at 110°C for 2h to obtain powdered NH 2 -MCM-48; 3) Add 48mL of ultrapure water to the Erlenmeyer flask, ...

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Abstract

The invention relates to a preparation method of a prostate tumor marker immunosensor. The preparation method comprises the following steps: (1) dissolving silver-hybridized NH2-MCM-48 into a phosphate buffered solution, uniformly dispersing in an ultrasonic mode, further adding a prostate specific antigen second antibody, oscillating at the temperature of 4 DEG C, centrifuging to remove the supernate to obtain a second antibody marker Ag@NH2-MCM-48 / Ab2; (2) firstly, modifying the surface of a glassy carbon electrode as a working electrode by using an amino graphene-ferrocenecarboxaldehyde composite material solution, subsequently modifying by using glutaraldehyde, further modifying by using a prostate specific antigen-anti-solution, dropping a bovine serum albumin solution, continuously modifying prostate specific antigen, and finally modifying Ag@NH2-MCM-48 / Ab1 so as to obtain the prostate tumor marker immunosensor. The immunosensor prepared by using the preparation method is applied to measurement on the prostate specific antigen, and is high in sensitivity and good in specificity.

Description

technical field [0001] The invention relates to a preparation method and application of a prostate tumor marker immunosensor, and belongs to the technical field of novel nanometer functional materials and biosensing. Background technique [0002] Tumor is a long-term effect of internal and external pathogenic factors on human tissue cells, which leads to mutations in the genetic material of the cells - deoxyribonucleic acid (DNA), which makes the growth and division of cells out of control, resulting in abnormal proliferation and function. out of tune. Tumor is a serious threat to people's health, and it is a major research topic that has received much attention in the medical field in recent years. [0003] Tumor markers refer to the abnormal expression of active substances produced by tumor tissues and cells. Tumor markers are not produced or produced very little in normal tissues or benign diseases, but in the tissues, body fluids and excreta of tumor patients. There is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/531
CPCG01N33/54393G01N33/57434
Inventor 李月云韩健魏琴董云会任祥陈润海
Owner SHANDONG UNIV OF TECH
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