78 results about "Aggregation phenomenon" patented technology

The invention relates to a convolutional neural network prediction method based on a rotation region. The method comprises steps that step 1, a convolutional neural network characteristic graph and an area of interests based on a rotary rectangular frame are generated according to input images; step 2, a rotary area-of-interest pooling layer is utilized to acquire a characteristic mapping graph according to the convolutional neural network characteristic graph and the area of interests; step 3, the characteristic mapping graph is mapped to be a one-dimensional characteristic vector; step 4, the one-dimensional characteristic vector is classified to acquire a classification result; step 5, rotary rectangular frame regression prediction of the one-dimensional characteristic vector is carried out to acquire a regression prediction result; and step 6, a final prediction result is outputted according to the classification result and the regression prediction result. The method is advantaged in that a problem of positioning and identification difficulties caused by target rotation and an aggregation phenomenon of images is solved, and precise positioning is realized.

A capacitive humidity sensor includes: a humidity sensing element with a humidity sensitive film and electrodes; and a processing circuit. The film has a dielectric constant variable in accordance with moisture. The electrodes detect capacitance of the film and outputs a signal corresponding to the capacitance. The processing circuit processes the signal. The film is made of inorganic material having fine pores for adsorbing the moisture. A diameter of the fine pores is set to be a predetermined diameter so that capillary tube aggregation phenomenon occurs at a predetermined relative humidity. The capacitance of the film has a change rate with respect to the relative humidity. The change rate in case of the relative humidity higher than the predetermined relative humidity is larger than that in case of the relative humidity lower than the predetermined relative humidity.

The invention relates to an in-situ preparation method for a functional nullvalent nanometer iron / polyelectrolyte composite fibrofelt. The method comprises the following steps: (1) preparing polyelectrolyte mixed solution with concentration between 7 and 12 percent by polyacrylic acid PAA, and preparing a nanometer fibrofelt according to a principle of electrostatic spinning; (2) carrying out heat treatment on the nanometer fibrofelt to prepare a water-fast polyelectrolyte fibrofelt; (3) preparing soluble iron salt solution, and preparing reducing agent solution with concentration 4 to 6 times of the iron salt solution; (4) dipping the (2) in the iron salt solution; (5) rinsing; (6) dripping the reducing agent solution onto the complex iron ion fibrofelt; (7) rinsing by deionized water; (8) drying and storing. In the fibrofelt prepared by the method, nanometer iron particles are dispersed evenly without aggregation phenomenon, and the fibrofelt can effectively fix nanometer iron particles, thereby ensuring effective recycle of the nanometer iron particles in practical application without secondary pollution.

The invention relates to a nanotechnology-based trace protein detection method, which combines enzyme-linked immunosorbent assay technology, tyramine signal amplification technology and the aggregation phenomenon of gold nanoparticles modified by different biological molecules, so an experimental method used for detecting trace proteins such as prostate specific antigen (PSA) and the like is established. The method comprises the following steps of: fixing an antibody aiming at the protein to be detected (such as the PSA) on the surface of a substrate; incubating another antibody with horseradish peroxidase (HRP) activity of the protein to be detected (such as the PSA) after capturing the protein to be detected in a sample, wherein the HRP catalyzes biotin-tyramide to generate biotin deposition under certain conditions; further amplifying a signal by using the aggregation phenomenon of the gold nanoparticles modified by biotin-labeled DNA and the gold nanoparticles modified by streptavidin; performing silver staining; and performing data analysis on an experimental result by using software. The method has the advantages of extremely low detection limit, wider detection range, capacity of detecting the antigen in a rabbit serum with complex compositions, and important application prospect.

The invention belongs to the technical field of nanometer material, and discloses a method for preparing a metal bismuth nanoparticle. The method comprises the following steps: mixing soluble bismuth salt aqueous solution and modifying agent organic solution; adding reducing agent into the mixture to be stirred, heated and refluxed; keeping the mixture still until layered; taking the black organic phase layer; and removing the organic solvent to obtain black powder, namely the metal bismuth nanoparticle. The preparation method of the invention has low cost and accessible raw materials, simple and convenient process, low cost and higher yield, and is applicable to large-scale industrial production. The metal bismuth nanoparticle prepared by the method has uniform size without obvious hard aggregation phenomenon, can be stably dispersed in a plurality of organic media for long time.

The particle components may be collected by using a phenomenon that the particle components in the aqueous phase aggregate at the liquid-to-liquid interface. Both of the particle components and the dissolved components in the aqueous phase may be simultaneously collected if combined with liquid-liquid extraction phenomenon that the dissolved components in the aqueous phase are collected into the solvent phase through the liquid-to-liquid interface. The aggregation phenomenon of the particle components at the liquid-to-liquid interface may be promoted by using an emulsion flow method, a method of applying mechanical external forces (such as stirring and vibrating) or another method combining both the above-mentioned methods.

The invention discloses a camptothecin-antibody conjugate. The category of ADC (Antibody-Drug Conjugate) drugs can be used for effectively improving the drug load, meanwhile, the above aggregation phenomenon is avoided, drug PK (pharmacokinetics) is improved, and an ideal drug effect is obtained from an in vivo model.

The invention provides an IGBT device with an integrated voltage sampling function, and belongs to the technical field of power semiconductor devices. A channel in which a JFET structure is introducedin a body region is in a normally-closed state, the device is in a forward turning-on state, and carriers are stored in the body region, so that an electric conductance modulating action is strengthened, and the saturated turning-on voltage drop of the device is reduced; in a turning-off state of the device, the body region plays a floating field limiting ring role, so that an electric field aggregation phenomena at the bottom of a groove gate is weakened, and the voltage withstand reliability of the device is improved; the grid electrode and the source electrode of the introduced JFET structure are connected with a peripheral control circuit and a sampling port respectively, a voltage sampling function can be realized by using a mapping reaction between the voltage change of the source electrode of the JFET structure and withstanding voltage of an IGBT, and the forward blocking characteristic of the device cannot be damaged while an electric isolation effect is reached; and the adjustment for voltage sampling rate is realized by changing the grid electrode bias voltage of the JFET structure, so that the requirement of different application conditions on voltage sampling is met. The IGBT device is simple in sampling structure, and is compatible with an existing technology.

The invention discloses a method for performing laser braze welding on diamonds by using double beams. The method comprises the following steps: providing a matrix for performing braze welding on thediamonds and forming a V-shaped groove in the surface of the matrix; coating a brazing material layer for performing braze welding on the diamonds in the V-shaped groove; providing a wolfram wire mesh, arranging a plurality of grids for putting the diamonds in the wolfram wire mesh; putting the wolfram wire mesh above the brazing material; putting the diamonds into the grids of the wolfram wire mesh to ensure that the diamonds and the brazing material layer have certain contact height; symmetrically abreast arranging two laser heads in the welding direction; starting a laser welding system. Byutilizing the double laser beams, the brazing material layer is aslant input from the two sides of the diamonds, and laser braze welding is carried out. The invention also provides a system for performing laser braze welding on the diamonds by utilizing the double beams. According to the method and the system disclosed by the invention, burning loss oxidation and graphitization of the surfaces ofthe diamonds are reduced, the bonding strength between the brazing material layer and the diamonds is improved, the aggregation phenomenon of the diamonds is avoided, and a desired arrangement sequence of the diamonds can be obtained.

A capacitive humidity sensor includes: a humidity sensing element with a humidity sensitive film and electrodes; and a processing circuit. The film has a dielectric constant variable in accordance with moisture. The electrodes detect capacitance of the film and outputs a signal corresponding to the capacitance. The processing circuit processes the signal. The film is made of inorganic material having fine pores for adsorbing the moisture. A diameter of the fine pores is set to be a predetermined diameter so that capillary tube aggregation phenomenon occurs at a predetermined relative humidity. The capacitance of the film has a change rate with respect to the relative humidity. The change rate in case of the relative humidity higher than the predetermined relative humidity is larger than that in case of the relative humidity lower than the predetermined relative humidity.

The invention discloses a copper-doped ZnO-ZrO2 sosoloid catalyst and a preparing method and application thereof. The preparing method of the catalyst comprises the steps of preparing a metal and nitrate mixed solution through Cu(NO3)2.3H2O, Zn(NO3)2.6H2O and Zr(NO3)4.5H2O; at the reaction temperature of 50-90 DEG C, dropwise adding the metal and nitrate mixed solution and a Na2CO3 solution into areaction container, maintaining the reaction temperature, conducting stirring, and conducting ageing at the reaction temperature; conducting centrifuging to obtain a solid precipitate after a productis cooled; then utilizing deionized water for washing the solid precipitate; then drying the precipitate to obtain a catalyst precursor; putting the catalyst precursor into a tube furnace for calcination to obtain a catalyst parent. By means of the copper-doped ZnO-ZrO2 sosoloid catalyst and the preparing method and application thereof, the dispersity of active components in the catalyst is improved, the sintering and aggregation phenomena of the catalyst are effectively inhibited, and the carbon dioxide conversion rate and methyl alcohol selectivity are high.

The invention discloses a method for preparing modified graphene reinforced copper-based composite material. The method specifically comprises the following steps: uniformly mixing a copper nitrate solution with a graphene oxide solution, and then adding hydrazine hydrate for carrying out reduction reaction; then cleaning a mixed solution after reaction, and drying precipitate, thus obtaining modified graphene composite powder; then carrying out liquid-phase powder mixing on the modified graphene composite powder and copper powder, drying by evaporating, cold-pressing and sintering, thus obtaining the modified graphene reinforced copper-based composite material. According to the method disclosed by the invention, surface modification is carried out on graphene, and copper particles are inserted between graphene sheet layers, so that the graphene is not easy to generate an aggregation phenomenon when the graphene is mixed with matrix copper powder; during a sintering process, a sintering neck can be formed by the copper particles of the graphene sheet layers and matrix copper, so that strong graphene / copper interface bonding strength can be favorably obtained; the modified graphenereinforced copper-based composite material prepared by adopting the method disclosed by the invention is uniform in organization and higher in strength and electrical conductivity.

The invention provides a method for preparing an Ag / PMMA (polymethyl methacrylate) nano composite material. Particularly, in the method, PMMA is used as a polymer matrix; PMMA is the English abbreviation of polymethyl methacrylate; silver nitrate is used as an Ag source; PVP (Polyvinyl Pyrrolidone) is used as a dispersing agent; PVP is the English abbreviation of polyvinyl pyrrolidone; DMF (N,N-dimethyl formamide) is used as a solution solvent and a reducing agent; and DMF is the English abbreviation of N,N-dimethyl formamide. The method comprises the following steps of: heating the PMMA, the silver nitrate, the PVP and the DMF and performing a reaction to obtain Ag / PMMA nano sol containing Ag nano particles; then removing the reducing agent DMF in the Ag / PMMA nano sol by adopting a solvent precipitation method; drying the obtained product; and carrying out heating and forming on the dried product to obtain the Ag / PMMA nano composite material. The method adopts a simple preparation process, can effectively solve the problem of the aggregation phenomenon when the Ag / PMMA nano composite material is prepared by a conventional method and realizes accurate design, regulation and control of diameter of particles and topography of the Ag nano particles.

The invention provides a fluid stirring paddle. The stirring paddle comprises a spindle head, two helical paddle arms and a paddle bottom, wherein the spindle head is used for connecting a rotating shaft; the paddle bottom is connected with the bottoms of the two paddle arms; the left and right sides of the spindle head extend outwards to form two paddle shoulders; the lower lateral surfaces of the two paddle shoulders are connected to form a horizontal plane; the two ends of the paddle shoulders extend downwards to form the two paddle arms, the paddle shoulders extend outwards from the middle of the spindle head, and the heights of the two paddle shoulders are gradually reduced from inside to outside; the upper left and right sides of the spindle head extend outwards to form at least two upright posts with gradually-reduced thicknesses; the bottoms of the upright posts are connected with the tops of the paddle shoulders; the cross sections of the upright posts are triangular; and the cross section of the paddle bottom is in a shape of a semi circle of which the bottom is inclined to the rotation direction. The stirring paddle has the advantages of effectively avoiding the 'aggregation phenomenon', 'clustering phenomenon' and 'pole climbing phenomenon', ensuring the excellent stirring effect of materials upon stirring and mixing the materials and fully fulfilling the aim of effectively stirring and mixing industrial fluid materials.

The invention discloses a quantum dot-doped functional boron nitride polymeric heat conducting compound film which is prepared from the following components in percent by weight: 1-5wt% of boron nitride quantum dots, 5-50wt% of functional boron nitride and 50-95wt% of a polymeric matrix. The invention also discloses a preparation method of the quantum dot-doped functional boron nitride polymeric heat conducting compound film. The preparation method of the quantum dot-doped functional boron nitride polymeric heat conducting compound film is reasonable in process, mild in reaction condition, favorable in production control and popularization and easy for industrial production. According to the prepared compound film, the functional boron nitride reduces the surface polarity of a nanosheet, so that the aggregation phenomenon of boron nitride is reduced. The boron nitride quantum dots are taken as filling particles among the boron nitride sheets, so that the sheets are linked more compactly, an integral efficient heat conducting network is formed by means of the boron nitride polymeric compound material, and the heat conducting property of the compound material is improved. The compound film has excellent mechanical properties, certain transparency and bending resistance.

Provided is a semiconductor nanoparticle-encapsulating vinyl polymer including vinyl polymer particles; and semi-conductor nanoparticles, uniformly dispersed in the vinyl polymer particles, having an average particle size of 1 to 150 nm, wherein the semiconductor nanoparticles are encapsulated by the vinyl polymer particles. Provided is also a mixture of the semiconductor nanoparticle-encapsulating vinyl polymer with a commercially available vinyl polymer. In the nanoparticle-encapsulating vinyl polymer and the mixture, since the semiconductor nanoparticles are encapsulated by the vinyl polymer particles, they are highly dispersed even in vinyl polymer products. Therefore; an aggregation phenomenon of semi-conductor nanoparticles that may be caused by physical mixing of semiconductor nanoparticles and a commercially available vinyl polymer can be prevented, thereby remarkably increasing a reduction in dioxin emission during incineration of the wastes of vinyl polymer products. Furthermore, the semiconductor nanoparticles of the semiconductor nanoparticle-encapsulating vinyl polymer can remarkably increase photodegradation efficiency due to the photocatalytic activity of the nanoparticles. In addition, the semiconductor nanoparticles of the semiconductor nanoparticle-encapsulating vinyl polymer can serve as fillers, thereby enhancing mechanical properties such as tensile strength and modulus of elasticity without lowering impact strength. In particular, in a flexible poly vinylchloride compound manufactured using semiconductor nanoparticles-encapsulating polyvinylchloride and a commercially available phthalate-based low-molecular weight liquid phase plasticizer, a plasticizer migration phenomenon can be prevented by adsorptivity of highly dispersed semiconductor nanoparticles.

The invention provides an identification method and system of a new building. The method comprises the following steps: obtaining engineering vehicles with stay behaviors; marking stay points of the engineering vehicles; obtaining a first wheelpath corresponding to each engineering vehicle with a stay behavior; obtaining a corresponding wheelpath set; determining whether a wheelpath aggregation phenomenon exists in the first wheelpath set; if so, obtaining a corresponding first driving area; determining whether the quantity of the stay points marked in the first driving area is greater than a preset stay point quantity threshold; and if so, matching the first driving area with map data to obtain a area where the new building is disposed. According to the invention, information of the new building in a specific area can be identified relevantly according to different demands of users so that convenience is provided for a third party, a traffic administration department or a navigation system.

The invention discloses a matrix modifier for determining heavy metals absorbed by graphite furnace atoms, and a preparing method. The matrix modifier is colloid palladium, and the preparing method of the matrix modifier comprises the following steps of: weighing 0.024g-0.036g of palladium chloride, and placing into a reacting container; converting into H2PdC14.nH2O by using concentrated hydrochloric acid; adding 0.030g of polyvinylpyrrolidone; then, slowly adding 60mL of mixing solution of methanol and water, continuously stirring and uniformly mixing in the adding process, placing the solution into a water bath tank for refluxing by heating for 90 minutes, and then taking out a brown colloid solution which is the target palladium. The novel matrix modifier can achieve high podzolizing temperature, the interference matrix can be effectively removed in the podzolizing stage, and the purpose for reducing or eliminating the interference is achieved. The colloid palladium prepared by the invention has uniform particle distribution, and no obvious aggregation phenomenon.

The present invention provides a preparation method of a cerium-doped rare earth silicate polycrystalline powder, and belongs to the technical field of preparation technologies of a rare-earth compound luminescent powder. The preparation method solves the technical problems that in the preparation process of an existing cerium-doped rare earth silicate polycrystalline powder, organic solvents areused, impurity elements are easy to introduce, the operation process is complicated, the reaction period is long, and the performance, such as light output, of a subsequent lutetium silicate crystal material is affected. According to the preparation method for the cerium-doped rare earth silicate polycrystalline powder provided by the present invention, sodium metasilicate is employed as a siliconsource, ammonia water or ammonium carbonate is taken as a precipitating agent, homogeneous co-precipitation is carried out for obtaining a mixed rare earth silica gel and a precipitation precursor, and finally calcination is carried out, so that the cerium-doped rare earth silicate polycrystalline powder is obtained. The method adopts a liquid phase co-precipitation process to prepare the pure-phase polycrystalline powder, the elements are uniformly distributed, no aggregation phenomenon is caused, no other organic reagents are introduced, introduction of impurity elements is reduced, the process flow is simple, consumption of chemical raw materials is low, the cost is low, and the method is easy to control and amplify.

The invention relates to an electrode plate for a fuel cell and the fuel cell thereof. The electrode plate comprises a substrate with at least one main acting plane, wherein at least one flow channel is arranged on the substrate, and a plurality of diversion projections staggeredly extend out of the two side walls of at least one flow channel at intervals; and a gap is formed between each diversion projection and the other side wall. Further, each diversion projection has a front curved surface, a back curved surface and a top surface, the front curved surface is arranged towards the converse direction of reaction flow, and the back curved surface is arranged towards the same direction of the reaction flow. Further, the front curved surface is of a concave surface, and the back curved surface is of a convex surface. Through the diversion projections, when the reaction flow passes through the diversion projections, the pressure of the reaction flow can be effectively increased, thereby not only improving the effect that the reaction flow penetrates the breathable layer of a membrane electrode assembly and effectively improving the distribution of the reaction flow, but also preventing the large block aggregation phenomenon generated by the reaction, and improving the reliability of the fuel cell.

The invention relates to a method for preparing a polyvinyl alcohol rice straw composite material. The method comprises processes of treating rice straw material, preparing coating liquor, preparing polyvinyl alcohol-coated rice straws, carrying out semi-drying and hot pressing and demolding. In the coating process, dosage of a solvent adopts double restraints, so that not only is sufficient coating ensured, but drying time and energy are saved. The semi-drying method is adopted for not only lowering energy consumption, but also promoting adhesion of rice straws in the hot-pressing process. Relative to a direct-mixing hot-pressing process, the method is used for solving an aggregation phenomenon of polyvinyl alcohol in the composite material, so that the prepared composite material is uniform in material composition, structure and performance, good in color and luster, high in strength and environment-friendly in process. The prepared composite material can be used for manufacturing articles of daily use, ornaments as well as agricultural auxiliary products, also can be further compounded with wood or plastics to produce the articles of daily use, ornaments as well as agricultural auxiliary products which are higher in cost performance.

The invention relates to a preparation method of a perylene pigment. According to the method, the reaction temperature between the organic solvent and solute is firstly preheated at 40-60 DEG C, and the samples are thoroughly mixed and react completely, thereby lowering the temperature increase speed, reducing the aggregation, being beneficial to crystallization, facilitating cleaning and saving the cleaning cost. When the temperature is higher than 70 DEG C, the reaction quickly happens, thereby not being beneficial to the sufficient reaction of the reactants, causing many unreacted substances, not being beneficial to forming crystals with uniform size, and aggravating aggregation; and the prepared product has coarse and large particles, and the aggregation phenomenon is not beneficial to impurity cleaning. The preparation method provided by the invention omits the ball milling after-treatment, and thus, is environment-friendly and economical. The prepared perylene pigment has the advantages of uniform crystal grains, favorable dispersity and high covering power, and thus, can be directly used in paints conveniently.

The invention discloses a metal demolding agent which comprises the following raw materials in parts by weight: 70-90 parts of fluoride graphite, 5-25 parts of graphite, 1-3 parts of a dispersing agent and 0.1-0.5 part of a fluorinated surfactant. The metal demolding agent disclosed by the invention has the main components of fluoride graphite and graphite, the two substances are high in melting point and high in lubrication property, then a dense lubricating membrane can be formed on the surface of a mold, the flowability of a metal in the mold can be improved, and the smoothness and the flatness of the surface of a cast part can be maintained; due to addition of the dispersing agent, not only is the aggregation phenomenon of a powder demolding agent solved, but also the demolding capability of a metal cast part can be improved; due to addition of the fluorinated surfactant, not only is the viscosity of the metal cast part reduced, but also effective spreading of the fluoride graphiteand the graphite can be ensured; through the coordinated use of the substances in a certain ratio, the thermal resistance of the demolding agent can be improved, high chemical stability and excellentlubricating properties can be maintained, and both the cast part and the mold are free of residue.

Provided is a semiconductor nanoparticle-encapsulating vinyl polymer including vinyl polymer particles; and semi-conductor nanoparticles, uniformly dispersed in the vinyl polymer particles, having an average particle size of 1 to 150 nm, wherein the semiconductor nanoparticles are encapsulated by the vinyl polymer particles. Provided is also a mixture of the semiconductor nanoparticle-encapsulating vinyl polymer with a commercially available vinyl polymer. In the nanoparticle-encapsulating vinyl polymer and the mixture, since the semiconductor nanoparticles are encapsulated by the vinyl polymer particles, they are highly dispersed even in vinyl polymer products. Therefore; an aggregation phenomenon of semi-conductor nanoparticles that may be caused by physical mixing of semiconductor nanoparticles and a commercially available vinyl polymer can be prevented, thereby remarkably increasing a reduction in dioxin emission during incineration of the wastes of vinyl polymer products. Furthermore, the semiconductor nanoparticles of the semiconductor nanoparticle-encapsulating vinyl polymer can remarkably increase photodegradation efficiency due to the photocatalytic activity of the nanoparticles. In addition, the semiconductor nanoparticles of the semiconductor nanoparticle-encapsulating vinyl polymer can serve as fillers, thereby enhancing mechanical properties such as tensile strength and modulus of elasticity without lowering impact strength. In particular, in a flexible poly vinylchloride compound manufactured using semiconductor nanoparticles-encapsulating polyvinylchloride and a commercially available phthalate-based low-molecular weight liquid phase plasticizer, a plasticizer migration phenomenon can be prevented by adsorptivity of highly dispersed semiconductor nanoparticles.

The invention discloses an amplitude-based sea radar multi-objective fusion tracking method. The method comprises the following steps of obtaining a current moment measurement value; performing processing on tracks of all the targets respectively; performing fusing and orienting on the measurement values based on the amplitude information; performing track fusion orientation based on the amplitudeinformation and the track quality; performing predicting and updating on the target state information, wherein each target state is updated according to the data interconnection probability parameters of all the targets, and the state information of each target at the k moment is obtained. The sea-radar multi-target fusion tracking method is used in the multi-target tracking process of the sea surface under the complex condition, and non-kinematics information such as amplitude information, used as representative of the target feature, is utilized, the amplitude information is compared with the set threshold parameter to judge the effectiveness of measurement, so that a track aggregation phenomenon caused by the fact that the target distance is close can be avoided, target measurement andclutter can be effectively distinguished, error correlation is reduced, and high-precision stable tracking of a sea surface target is realized.

A carbon nitride / graphene composite electrode material comprises the following components in percentage by weight: 0.1 to 1.0 percent of carbon nitride and 99.0 to 99.9 percent of graphene oxide, wherein the carbon nitride is used as a framework structure and the graphene oxide is wrapped on the surface of the carbon nitride to form a core-shell structure. The invention has good electrochemical performance, having a high Faraday capacitance, Faraday capacitance is 221 F / g at a scan speed of 5 mV / s, the stability of battery and electrode is good in short-range charging and discharging, in ac impedance teste, Materials reflect a small impedance value, materials in the charge and discharge tests show a small polarization, and compared with the traditional complex treatment of composite materials, the composite method is simple, safe and reliable production process, large capacitance, preparation process will not meet graphene aggregation phenomenon, preparation is simple, production cycleis short, and market promotion and application are facilitated.

The invention discloses an autophagy monitoring method for fat cells. Due to the adoption of a special structure, mature fat cells are full of plenty of lipid droplets, so that organelle and nucleus are displaced towards the periphery, and a typical autophagosome is hard to be seen under an electron microscope. Proved by a plurality of experiments, the method disclosed by the invention finally and successfully observes autophagy structures in various autophagy stages in mature 3T3-L1 fat cells by continuously improving the experiment condition. Besides, mature fat has a characteristic of being not easy to be transfected, so common plasmid transfection is hard to play an expectant effect. Therefore, the method disclosed by the invention utilizes a GFP-LC3 lentivirus method to infect 3T3-L1 fat cells, and the infection efficiency is as high as 80% or greater by observation under a fluorescence microscope. At nutrition and hunger condition, by the method disclosed by the invention, GFP-LC3 point-like aggregation phenomenon is successfully observed. The establishment of the autophagy monitoring method for mature fat cells lays the foundation of research on the relationship between autophagy and fat metabolism for us.

The invention discloses a method for preventing a pipeline from being blocked by too-wet dressing soil in slope dressing soil dry-spraying operation. The method includes the following steps that firstly, improved dressing soil is prepared, wherein a sand material and vegetable matter are added to the water-contained dressing soil to be stirred and evenly mixed; and secondly, the improved dressing soil is sprayed to a mountain slope beside a road through a dressing soil dry-spraying process. By the adoption of the method, a dressing soil ratio improving method is adopted, bonded particles are effectively scattered, the matrix wall bonding phenomenon is effectively avoided, viscosity is reduced, and the aggregation phenomenon is reduced; the wall bonding phenomenon is reduced; and the pipeline blocking phenomenon generated in dry-spraying operation can be effectively prevented, smooth construction is guaranteed, waste is used, cost is reduced, the soil is evenly sprayed to the slope face of the slope, the construction progress is guaranteed, and the afforesting effect of the slope can be guaranteed.

The invention discloses a preparation method of a surfactant-modified CuInS2 nanocrystal, which comprises the following steps: sequentially adding CuCl2.2H2O, InCl3.4H2O and CH4N2S into an organic solvent, sufficiently dissolving by stirring, adding a surfactant, and evenly mixing, wherein the surfactant is a KH550 silane coupling agent, KH570 silane coupling agent or polyvinyl butyral; transferring the mixture into a pressure kettle, sealing, carrying out thermostatic reaction for 12 hours, and after the reaction finishes, naturally cooling to room temperature; and centrifuging the reaction product, washing and drying. The binary mixed solvent is used instead of the traditional single organic solvent, and the surfactant is added into the mixed reaction system, thereby improving the aggregation phenomenon of the product, and obtaining the nano photovoltaic material which has the advantages of uniform nanosheet structure, high solid yield, favorable light absorption property and proper energy gap.