The invention discloses a method for determining the
nitrogen index of
protein in milk
powder and application, and the method comprises the following steps: taking a
milk sample, and dissolving in water to obtain an
emulsion; adjusting the pH value of the
emulsion to precipitate
casein and denatured
whey protein, and filtering to obtain a filtrate A; determining the
nitrogen content in the filtrate A; mixing the filtrate A with a
trichloroacetic acid solution to precipitate undenatured
whey protein, and filtering to obtain filtrate B; determining the
nitrogen content in the filtrate B; the difference value between the nitrogen content in the filtrate A and the nitrogen content in the filtrate B is the nitrogen content of the undenatured
whey protein in the milk
powder. The determination method disclosed by the invention has the characteristics of high universality, precision, accuracy,
operability and the like, and provides a technical basis for determining the denaturation degree of the whey
protein in the milk
powder.