74 results about "Protein nitrogen" patented technology

The invention relates to a culture medium for Haemophilus influenzae type b, characterized in that the source of protein nitrogen is of non-animal origin and comprises at least one plant peptone and in that the heme source consists of protoporphyrin IX. This medium serves in particular for the production of polyribosyl phosphate (PRP) and for the manufacture of a vaccine against Haemophilus influenzae type b meningitis.

The invention discloses a method for recovering coin protein sugar dregs and preparing protein nitrogen sources and nitrogen-containing syrup in the production of enzymatic corn starch sugar, comprising the steps of size mixing, liquefying, saccharifying, standing separation, centrifugal separation, proteolysis, concentration, drying, compounding, and the like. In the invention, protein is condensed under the heat flash of liquefying, the protein sugar dregs concentrate and float upwards in saccharifying, the sugar dregs are recovered through the steps of standing separation and centrifugal separation, the sugar dregs are dried into protein, or the sugar dregs are hydrolyzed to prepare protein nitrogen sources, and the nitrogen sources can be compounded with syrup to form nitrogen-containing starch syrup for fermenting. Clarified saccharification liquid after centrifugal separation is decolored, filtered, refined and concentrated, and starch syrup products are obtained. The majority of lentous sugar dregs in the saccharification liquid are removed before the treatment of decoloring and filtering, and the decoloring and filtering performance of the material is better. The use level of active carbon, and the like can be properly reduced, the production capability of filtering equipment is improved, and the frequency of the loading and unloading of filters as well as the consumption of washing waste water and various kinds of loss are greatly reduced.

The invention discloses a method for preparing organic nitrogen source applicable to fermentation and food by taking rice or rice flour as raw material and carrying out acid process or enzymatic hydrolysis for scum left through pressing and filtering in the process of filtering starch syrup produced in an enzymatic method. The method comprises the following steps: the rice or the rice flour is washed and ground to be milk, calcium chloride and liquefying amylase are added to the milk for consecutive spraying liquefaction to obtain liquefied liquid, various saccharifying enzymes and/or germinating malts and lipase are added to the liquefied liquid for synergistic saccharification, the starch syrup is obtained by filtering saccharified product and refining and concentrating filtrate; the scum obtained by filtering and pressing is washed by water, the residual sugar in the scum is obtained by filtering and pressing, and the washing liquor is used for size mixing; alkaline lipase is added to the left washed sugar-free grains to further hydrolyze the fat in material, rice sugar-free grains protein with higher protein content is obtained by filtering and washing, and rice sugar-free grains protein is hydrolyzed by edible hydrochloric acid or protease to prepare the organic nitrogen source which can be used as the fermentation raw material of a biopharmaceutical process.

The invention discloses a measuring method of the protein nitrogen content in tobaccos, which comprises the following steps: (a) the tobaccos are weighed; (b) the weighed tobaccos are placed into adequate acid or copper sulfate solution, and heated until protein is completely solidified; (c) after the complete protein solidification, filtering is implemented, and filtered materials are rinsed with the acid or the copper sulfate solution in step (b); (d) filtering liquids are mixed and volume is limited; (e) the filtering liquids are processed by digesting and volume is limited; (f) the processing of a standard ammonium salt solution is implemented with the same digestive treatment in step (e), and volume is limited; (g) color comparison of the filtering liquids obtained from step (e) and the solution obtained after the volume limiting in step (f) is implemented, thus obtaining the nitrogen content in the filtering liquids; and (h) the total nitrogen content in the tobaccos is measured, wherein, step (f) can be reversed with steps (a) to (e), and step (h) can be reversed with steps (a) to (g). The measuring method provided by the invention is the simple, convenient and high-efficient method for measuring the protein nitrogen content in the tobaccos.

The invention provides a fermenting enzymolysis agent for soybean meal fermentation and application thereof and belongs to the technical field of feed fermenting agents. The fermenting enzymolysis agent consists of microorganism strains, enzyme preparations, an accessory ingredient and a carrier. According to the fermenting enzymolysis agent, seven types of beneficial bacteria and three types of enzyme preparations are combined, 72-hour aerobiotic, microaerobic and anaerobic fermentation and enzymolysis are performed on crushed soybean meal and a small amount of corn flour, so that antinutritional factors in the soybean meal are eliminated, cell walls are broken, non-protein nitrogen is converted into mycoprotein, proteins are decomposed into polypeptide and small peptide, more Vitamin B is generated, the nutrition performance of the soybean meal is comprehensively improved, and thus the soybean meal becomes a feed protein source capable of partially replacing a feed protein source for import fish meal feed.

The present invention discloses one kind of sheep feed and its production process. Pre-mixed material is first produced with zinc sulfate, ferrous sulfate, anhydrous cupric sulfate, potassium iodide, sodium selenite, cobalt chloride, manganese sulfate, vitamin A, vitamin D3 and vitamin E; and then mixed with slow released non-protein nitrogen, calcium biphosphate, alcoholic dregs, feather powder, cotton seed dregs, sunflower seed dregs, tree leaf powder, calcium carbonate, anhydrous sodium sulfate, sodium bicarbonate and yeast through stirring homogeneously to produce the sheep feed. The sheep feed has balanced nutrients, protein capable of being decomposed directly in the rumen of sheep, high absorption rate and raised sheep growing speed.

The invention discloses biological feeds for sea cucumber, which comprise the following components by weight portion: vegetable protein accounting for 10 to 20 parts, straws accounting for 30 to 50 parts, bur clover accounting for 10 to 20 parts, pomace and peelings accounting for 10 to 30 parts, pot ale accounting for 5 to 10 parts, zymocyte accounting for 0.02 to 0.05 parts and complex enzyme accounting for 0.02 to 0.05 parts. The sea cucumber biological feeds can resolve polysaccharide substances such as robust fibers to produce low-molecular monosaccharide, hexabiosa and the like, can improve nutritional components of the feeds, can accelerate the feed acidation, can promote vegetable protein to convert to microbial protein nitrogen, can improve the digestion utilization ratio, can reduce feed coefficient, can achieve good food calling performance, and can accelerate the growth rate; the sea cucumber biological feeds contain a great amount of probiotics groups and active enzyme, so that propagation and growth of destructive bacteria, mixed bacteria and putrefying bacteria can be inhibited, water contamination caused by putrefaction of residual bait can be controlled, water environment with sufficient oxygen can be provided to sea cucumber, the immunization and the disease resistance of sea cucumber can be improved, the survival rate is improved, and output benefits can be improved further; in addition, raw materials are abundant and are easy to get, and the cost of the feeds is low.

The present invention discloses one kind of non-protein nitrogen feed additive for ruminant and its production process. The non-protein nitrogen feed additive has reasonable recipe, easy production, low cost, high decomposition speed, urea decomposing speed matching the utilization speed of rumen to avoid ammonia accumulation in blood and good taste. The non-protein nitrogen feed additive consists of urea 35-50 wt%, corn starch 35-48 wt%, and coating material 2-20 wt%, and its production process includes the steps of dispersing urea and corn starch in coating material solution, grinding in a colloid mill to form emulsion, and spray drying at normal temperature to form microspherical feed additive. It may be also produced into powder, granule, tablet or capsule.

The present invention discloses one kind of milk cow feed. Pre-mixed material is first produced with zinc sulfate, ferrous sulfate, anhydrous cupric sulfate, potassium iodide, sodium selenite, cobalt chloride, manganese sulfate, molybdenum chloride, vitamin A, vitamin D3 and vitamin E and through mixing; and then mixed with slow released non-protein nitrogen, calcium biphosphate, feather powder, cotton seed dregs, tree leaf powder, calcium carbonate, table salt, anhydrous sodium sulfate, sodium bicarbonate, yeast and vegetable oil through stirring homogeneously to produce the milk cow feed. The milk cow feed has balanced nutrients, protein capable of being decomposed directly in the rumen of milk cow, high absorption rate and capacity of raising milk yield.

The invention discloses lactobacillus plantarum Lp1 and an application thereof in preparation a feed additive. The disclosed lactobacillus plantarum Lp1 has a preservation number of CGMCC No. 10473. The invention discloses the application of the lactobacillus plantarum Lp1 in preparing alfalfa silage. The lactobacillus plantarum LP1 can be used as the alfalfa silage additive, and has the advantages of significantly increasing output of lactic acid, reducing the pH value significantly and effectively improving fermentation quality of the alfalfa silage; the content of non-protein nitrogen and ammonia nitrogen can be reduced, degradation of protein in the alfalfa silage is inhibited, and feed value of the alfalfa silage is increased effectively; meanwhile, the lactobacillus plantarum LP1 has the advantages of high fermentation efficiency, low cost and the like and can be applied to production of green bio-feed.

The present invention relates to a fermented composition, and particularly relates to a fermented crop straw composition and an application thereof. The fermented crop straw composition includes the following components in parts by weight: 5-10 parts of molasses, 8-10 parts of compound bacteria, 2.5-4.5 parts of compound enzyme, 4-6.5 parts of compound trace elements, 0.5-2 parts of non-protein nitrogen, and 0.5-1.0 part of edible salt. The compound bacteria are cultured and blended by 12%-17% of candida utilis, 7%-12% of bacillus subtilis, and 8%-12% of trichoderma viride. The composition is used to ferment crop straws and to prepare ruminant feed, the crop straws contain a large amount of cellulose which is decomposed, the crude fiber content is decreased, the fermented feed crude protein content is enhanced, the palatability is improved, the digestibility is improved, and at the same time, the cost of feed is reduced .

The invention discloses a milk powder protein nitrogen content analysis method belonging to protein check technique. Based on the chromatometry theory combined with that trichloroacetic acid can deposit protein and biuret can make protein into violet complex compound, that the sample with different protein contents via trichloroacetic acid can deposit the protein nitrogen, and the protein nitrogen under alkali condition can form bluish violet complex compounds with different color degrees via the biuret agent, and the chromatic value is linearly correlative with the protein content, the invention uses a computer software automatic analysis to obtain the value to be compared with the absorbance tested by a spectrophotometer, to calculate out the protein content. The invention uses general computer and scanner to replace special lab precision device as spectrophotometer, with the processes as accuracy, time, and cost test but without artificial standard curvature drafting, to make operation quick and simple, save device, reduce cost, and reduce condition demand, without pollution, while the invention can be used to detect milk powder in batch.

The invention discloses a method for manufacturing raw materials of fodder with rich proteins from amino acid fermentation residual liquor. The method includes inoculating and cultivating candida tropicalis or candida utilis and inoculating the candida tropicalis or the candida utilis into the amino acid fermentation residual liquor to obtain fermentation mash; adding honey into soybean meal and the like to manufacture solid fermentation raw materials; proportionally mixing the fermentation mash and the solid fermentation raw materials with one another, then carrying out solid-state fermentation on the fermentation mash and the solid fermentation raw materials, and drying and crushing the fermentation mash and the solid fermentation raw materials. The method has the advantages that a characteristic that residual non-protein nitrogen and residual sugar in the amino acid fermentation residual liquor can be converted into microbial cells by the candida tropicalis or the candida utilis is utilized, and the candida tropicalis or the candida utilis is inoculated into the amino acid fermentation residual liquor, so that the non-protein nitrogen and carbon sources which remain in the amino acid fermentation residual liquor during amino acid production can be converted into proteins of the microbial cells by the aid of the candida tropicalis or the candida utilis, and resources of the amino acid fermentation residual liquor can be sufficiently utilized; the non-protein nitrogen utilization rate further can be increased owing to solid-state fermentation, accordingly, the cost of the fodder can be reduced, and the method for efficiently manufacturing the raw materials of the fodder with the proteins is provided for developing the breeding industries.

A feed for ruminant animal (ox, sheep, deer, etc) contains the pre-mixture of ferrous sulfate, zinc sulfate, calcium carbonate, VA, VB, straw, etc, the concentrated material of non-protein nitrogen, pine needle powder, stone powder, etc. trace elements, and thionitrogen. Its advantages are high absorption rate (95-100%), low cost, and high quality of meat.

A slow-releasing non-protein nitrogen feed for domestic animals and fowls is prepared from corn flour, urea, bentonite, brown sugar and soybean through pulverizing, proportionally mixing, puffing, cooling and breaking. It features that the urea particles are wrapped by starch membrane to elongate its hydrolyzing time to 3-4 hr, so preventing poisoning.

The invention discloses a detection reagent, a detection method and a detection instrument for proteins. The reagent is prepared by the following steps: dissolving 0.1 to 10 weight parts of acid orange-12 into 1 to 500 weight parts of buffer solution of phosphoric acid; adding 1 to 500 weight parts of glacial acetic acid; and finally, diluting with 1 to 5,000 weight parts of water, and oscillating and mixing to obtain the detection reagent for the proteins. Compared with the traditional method, the detection method can effectively detect the content of true proteins, and detect the proteins in a wide range; and the detection accuracy is basically not interfered by non-protein nitrogen-containing compounds such as carbamide and melamine. The instrument has small structure, and is convenient to carry. The detection reagent, the detection method and the detection instrument can be widely applied to quick detection of milk products in the basic level and on the spot.

The invention provides a method for measuring the protein nitrogen content in tobaccos. The method comprises the following steps of: separating protein nitrogen in the tobaccos to obtain a sample to be measured, wherein the sample to be measured contains non-protein nitrogen; adding potassium peroxydisulfate to the sample to be measured under the heating condition to obtain a mixed solution; carrying out ultraviolet catalysis and high-temperature heating on the mixed solution in sequence to obtain a digestion product; detecting the digestion product to obtain the non-protein nitrogen content in the tobaccos; and obtaining the protein nitrogen content in the tobaccos according to the preset total nitrogen content in the tobaccos and the non-protein nitrogen content in the tobaccos. The method provided by the invention has the capability of digesting nitrogenous substances in the tobaccos more completely and thoroughly and has a favorable digestion effect; and according to the method provided by the invention, reducible components in the tobaccos are also digested at the same time that the nitrogenous substances in the tobaccos are digested, so that the tobaccos lose the reducing action, the interference of the reducing components to the digestion of the nitrogenous substances is reduced, the homogeneity of digestion results is improved, and the accuracy of measuring the protein content in the tobaccos is also improved.

The present disclosure relates to a sterile medical product for parenteral nutrition comprising a polymeric container having at least a first and a second chamber which are separated by a non-permanent peel seal, wherein the first chamber contains a composition of amino acids and optionally electrolytes, and wherein the second chamber contains a dextrose solution, and wherein the product is characterized by a high protein (nitrogen) content per volume. The reconstituted solution is configured to be administered peripherally or centrally for the treatment of patients suffering from malnutrition and/or having a need for increased uptake of amino acids.

The invention discloses a <15>N SIP (stable isotope probing) vibrio parahaemolyticus culture medium and a cultivation method thereof. A formula of the culture medium comprises components as follows: 1-5 g/L of a nitrogen source, 5-18 g/L of a carbon source, 0.5-2.0 g/L of KH2PO4, 0.6-2.0 g/L of K2HPO4, 0.1-1.0 g/L of MgSO4, 10-30 g/L of NaCl and 1L of distilled water, the pH is in a range from 7.0 to 7.5, and nitrogen atoms of the nitrogen source are labeled by adopting <15>N. The cultivation method of the <15>N SIP vibrio parahaemolyticus comprises steps as follows: 1), continuously and repeatedly cultivating the vibrio parahaemolyticus through transferring; 2), verifying the labeling efficiency of protein nitrogen atoms in labeled vibrio parahaemolyticus cells with LC-MS/MS (high-performance liquid chromatography-tandem mass spectrometry). The process is simple and reasonable, the culture medium has simple components and is low in cost, and the novel culture medium preparation method is provided for industrial preparation of the <15>N SIP vibrio parahaemolyticus culture medium.

The invention discloses a method for preparing a fatty acid-rich protein feed raw material from amino acid fermentation residual liquor. The method comprises the following steps: performing inoculated culture on trichosporon mucoides first and then inoculating a trichosporon mucoides seed solution into the amino acid fermentation residual liquor to obtain fermented liquor; adding a proper amount of molasses into bean pulp and the like, and mixing uniformly to prepare a solid-state fermentation raw material; mixing the fermented liquor with the solid-state fermentation raw material at a certain ratio, and performing aerobic solid-state fermentation; drying a fermented material and grinding. Wastes are reasonably utilized, the utilization rate of the fermentation residual liquor in amino acid production is increased, the trichosporon mucoides capable of efficiently utilizing non-protein nitrogen is applied to the fermentation of the amino acid fermentation residual liquor, and residual non-protein nitrogen and sugar in the amino acid fermentation residual liquor are fully utilized for microbial conversion. Moreover, in combination with solid-state fermentation, the utilization rate of non-protein nitrogen is further increased, the drying cost is greatly reduced, the environment is protected, resources are saved, and the low-cost and fatty acid-rich protein feed raw material is obtained.

The invention discloses a method for rapidly detecting the content of protein in tobacco leaves, which comprises the following steps: taking a certain amount of tobacco leaf powder to be detected in a homogenizer, adding 0.05 mol/L phosphate buffer solution containing SDS (sodium dodecyl sulfate) with the pH value of 6.8, fully grinding to obtain homogenate, carrying out ultrasonic oscillation on the homogenizer, then carrying out centrifugal separation, then carrying out centrifugal separation at the rotating speed of 4800r/min, taking the supernatant, filtering, and carrying out freeze drying to obtain the protein content in the tobacco leaves. According to the method, cell tissues are fully damaged in the homogenizing process, exudation of protein is facilitated, protein can be efficiently separated out from the screened protein precipitation solution, the influence of non-protein nitrogen is avoided, and the result is more accurate; compared with a Kendall method and a continuous flow analysis method, the method has the advantages that the steps are simplified, the digestion time is obviously shortened, and the total consumed time is less; the ultraviolet and visible spectrophotometer can realize simultaneous detection of a plurality of samples and has the characteristics of high efficiency and large flux, and other devices such as a centrifugal machine and a water bath kettle are standing devices in a laboratory.

The invention discloses a sheep feed and its production technology. The production technology of the sheep feed mainly comprises the following steps: using zinc sulfate, ferrous sulfate, anhydrous copper sulfate, potassium iodide, sodium selenite, cobalt chloride, manganese sulfate, VA, VC and VD to prepare a premix, and uniformly mixing slow-release non-protein nitrogen with calcium hydrogen phosphate, distiller's grains, feather meal, cottonseed meal, sunflower meal, leaf powder, calcium carbonate, anhydrous sodium sulfate, sodium bicarbonate, yeast and the premix to prepare the sheep feed. A slow-release non-protein nitrogen technology and trace elements are adopted to realize sulfur and nitrogen balance, electrolyte balance, calcium and phosphorus balance, amino acid balance and osmotic pressure balance in order to make protein sources directly decomposed by sheep rumens, and the release of the slow-release non-protein nitrogen is synchronous with the development and absorption of thalli in the rumens, so the absorption rate increases to 96-98%, and the feeding cost is reduced; and beneficial bacteria are decomposed and absorbed by the rumen under the action of acetic acid, so long wool, rapid weight gain and economic benefit increase are reached.

The invention relates to the technical field of feed processing, in particular to anthocephalus chinensis leaf powder serving as a silage additive and application thereof in stylosanthes guianensis silage. According to the invention, the anthocephalus chinensis leaf powder is used as a silage additive, is added into stylosanthes guianensis silage, reduces the water content of the stylosanthes guianensis silage, remarkably reduces the pH value, propionic acid content, non-protein nitrogen content and ammoniacal nitrogen content of the silage, remarkably increases the number of lactic acid bacteria, lactic acid content, crude protein content and true protein content, and has a remarkable improvement effect on the improvement of the quality of the stylosanthes guianensis silage and on the protection of nutritional ingredients; the prepared stylosanthes guianensis silage can maintain the nutrition of stylosanthes guianensis, is rich in nutrition, mellow in smell, soft, juicy and good in palatability, and the quality of the stylosanthes guianensis silage is improved; the anthocephalus chinensis leaf powder is cheap, easy to obtain and simple and convenient to operate.

The invention discloses a straw organic matter cross-linked polypeptide chelated medium trace element slow-release fertilizer and a preparation method thereof, and relates to agricultural waste resource comprehensive utilization and functional fertilizer production technologies. The method comprises the following steps: dissolving, degrading and modifying agricultural wastes (crop straws and sargassum thunbergii) rich in cellulose resources and wastes (such as cephalopod processing leftovers, fish processing leftovers, livestock and poultry processing leftovers, oil processing leftovers and the like) rich in proteins; preparing a phosphorylated organic matter compound and a phosphorylated protein hydrolysate; carrying out cross-linking reaction to obtain straw organic matter cross-linked protein microspheres; then fixedly loading medium and trace elements; and performing pelleting to manufacture the slow-release fertilizer rich in organic matters, protein, nitrogen phosphorus potassium, medium/trace elements. According to the method disclosed by the invention, the porosity and the specific surface area of the fertilizer are greatly increased, so that negative charges on the surfaceof the fertilizer are increased, the complexing point location and the capability of chelating the medium trace elements are improved, the medium and trace element loading capacity is improved, and the steric hindrance and the slow release effect of the slow release fertilizer are enhanced.