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Lactobacillus plantarum Lp1 and application thereof in preparation of feed additive

A technology of Lactobacillus plantarum, LP1, applied in the direction of bacteria used in food preparation, preservation method of animal feed raw materials, Lactobacillus, etc., can solve the problem of low soluble carbohydrate content, difficulty in making semi-dry silage of alfalfa, and low protein feed. Use value and other issues to achieve the effect of improving feed value, reducing content and increasing lactic acid output

Inactive Publication Date: 2017-12-15
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, alfalfa is low in soluble carbohydrates and has strong buffering capacity, which is not conducive to silage lactic acid fermentation
[0003] Although reducing the water content of alfalfa silage by wilting can inhibit the fermentation of Clostridium and improve the fermentation quality, however, in North China and the Huanghuaihai region of China, it is difficult to produce semi-dried alfalfa silage by wilting in the field due to the heavy rainfall during the harvest season of alfalfa
In the initial stage of alfalfa silage fermentation, the lactic acid bacteria attached to the plant and Clostridium co-exist. When the water content of silage raw materials is higher than 60% and the ambient temperature exceeds 37°C, Clostridia are prone to spoilage, producing a large amount of butyric acid and ammonia nitrogen, which makes the pH The value cannot be lowered, causing a large amount of protein degradation to reduce the feeding value, and it is not conducive to long-term storage

Method used

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  • Lactobacillus plantarum Lp1 and application thereof in preparation of feed additive
  • Lactobacillus plantarum Lp1 and application thereof in preparation of feed additive
  • Lactobacillus plantarum Lp1 and application thereof in preparation of feed additive

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Example 1. Isolation, screening and identification of Lactobacillus plantarum LP1

[0054] 1. Isolation and screening of strains

[0055] Collect sheepskin soaking solution (the liquid after soaking raw hides in water during the production process of sheepskin) in Hohhot City, Inner Mongolia, shake it with an oscillator for 30 minutes, and use distilled water for 10 minutes. -1 -10 -6For gradient dilution, take 1mL from each dilution gradient dilution and inoculate it into a petri dish, add 10-15ml sterilized MRS solid medium, shake well, wait for it to cool and solidify, and let it stand for anaerobic culture for 48h. Pick single colonies with obvious differences in colony shape, size, color and gloss, and repeat streaking until pure colonies are obtained. Single colonies were picked for Gram staining and catalase test. All Gram-positive, catalase-negative strains were initially identified as lactic acid bacteria, and then a lactic acid bacterium with high growth an...

Embodiment 2

[0092] Embodiment 2, plant Lactobacillus (Lactobacillus plantarum) LP1 prepares alfalfa silage

[0093] 1. Bacteria powder preparation

[0094] Lactobacillus plantarum LP1 is inoculated in 10ml MRS liquid medium, 37 ℃, 250rpm shaking culture, after cultivating 2 generations, inoculate in 60ml MRS liquid medium by the inoculum size of 3% (volume ratio) (bacterial liquid initial OD 260nm =1.8), 37°C, 250rpm shaking culture for 18-24h (the final OD of bacterial solution 260nm = 4), the culture system is centrifuged, the thalline precipitation is collected, the thalline precipitation is mixed with 10ml sterilized skim milk and then freeze-dried to obtain a solid bacterial preparation, the concentration of Lactobacillus plantarum in the solid bacterial preparation is 1 × 10 12 CFUg -1 (Need to be greater than 1×10 10 CFUg -1 ).

[0095] 2. Preparation of alfalfa silage

[0096] Alfalfa: The last crop of alfalfa at the budding stage planted in Zhuozhou, Hebei was harvested on ...

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Abstract

The invention discloses lactobacillus plantarum Lp1 and an application thereof in preparation a feed additive. The disclosed lactobacillus plantarum Lp1 has a preservation number of CGMCC No. 10473. The invention discloses the application of the lactobacillus plantarum Lp1 in preparing alfalfa silage. The lactobacillus plantarum LP1 can be used as the alfalfa silage additive, and has the advantages of significantly increasing output of lactic acid, reducing the pH value significantly and effectively improving fermentation quality of the alfalfa silage; the content of non-protein nitrogen and ammonia nitrogen can be reduced, degradation of protein in the alfalfa silage is inhibited, and feed value of the alfalfa silage is increased effectively; meanwhile, the lactobacillus plantarum LP1 has the advantages of high fermentation efficiency, low cost and the like and can be applied to production of green bio-feed.

Description

technical field [0001] The invention relates to the technical field of forage feed processing and storage, in particular to a plant Lactobacillus LP1 and its application in the preparation of feed additives. Background technique [0002] Alfalfa (Medicago sativa L.), referred to as alfalfa for short, is the most widely cultivated leguminous grass in my country. It has high protein content and high nutritional value, and is known as the "king of grass". As an important form of alfalfa products, silage alfalfa can not only solve the loss caused by alfalfa hay processing and rain in my country, but also increase the diversity of alfalfa products and prolong the utilization of green and juicy feed. However, the low content of soluble carbohydrates in alfalfa has a strong buffer capacity, which is not conducive to silage lactic acid fermentation. [0003] Although reducing the water content of alfalfa silage by wilting can inhibit the fermentation of Clostridium and improve the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18A23K10/12A23K10/20C12R1/25
CPCA23K10/12A23K10/20A23K30/18C12N1/20C12R2001/25C12N1/205A23V2400/169
Inventor 玉柱吴哲侯建建张英俊杨富裕
Owner CHINA AGRI UNIV
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