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105 results about "Non-protein nitrogen" patented technology

Non-protein nitrogen (or NPN) is a term used in animal nutrition to refer collectively to components such as urea, biuret, and ammonia, which are not proteins but can be converted into proteins by microbes in the ruminant stomach. Due to their lower cost compared to plant and animal proteins their inclusion in a diet can result in economic gain, but at too high levels cause a depression in growth and possible ammonia toxicity (microbes convert NPN to ammonia first before using that to make protein.) NPN can also be used to artificially raise crude protein values, which are measured based on nitrogen content, as protein is about 16% nitrogen, but, for example, urea is 47% nitrogen. The source of NPN is typically a chemical feed additive, or sometimes chicken waste and cattle manure.

High-protein microbiological feed and preparation method thereof

The invention provides a high-protein microbiological feed and a preparation method of the high-protein microbiological feed. Dross is one of products produced in the process of floating and clarifying the mixed juice during sugar preparing by the sugarcane, and is rich in various organic non-sugar components; the dross is always considered as impurities and mixed in the mud juice by a sugar plant, and finally becomes lime sludge to cause waste. The preparation method provided by the invention comprises the following steps of taking the dross in the crane sugar plant as the raw material, and taking a non-protein nitrogen source, phosphate and magnesium salt and the like as the auxiliary materials; mixing the raw material and the auxiliary materials; sterilizing; adding beneficial zymocyte preparation; and fermenting at the aerobic condition so as to obtain the high-protein microbiological feed. By adopting the preparation method provided by the invention, the problems that a large quantity of dross is in the crane sugar plant and is adhered and hardly processed, and the loss of the sugar is serious can be solved well; and the current situation that the protein feed resources are in a shortage can be relieved. According to the preparation method, the cost is low, the period is short, the production technology is simple, the limitation from the field and equipment is less, and the quality and the yield of the high-protein microbiological feed are high, and the preparation method plays an important role in popularization and application of the mixed juice floating and clarifying technology in sugar plants.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION CENT FOR ANALYSIS & TEST RES +1

Systems and methods related to degradation of uremic toxins

The present invention generally relates to the treatment of uremic toxins in vivo using uremic toxin-treating enzymes, and/or cells capable of producing uremic toxin-treating enzymes or otherwise reacting with uremic toxins. Non-limiting examples of cases where the treatment of uremic toxins is desired include renal disease or dysfunction, gout, subjects receiving chemotherapy, or the like. In one aspect, the treatment includes an oral delivery composition able to reduce the blood concentration of one or more non-protein nitrogen compounds in vivo. The composition, in some cases, may comprise one, two, or more uremic toxin-treating enzymes, such as urease, uricase or creatininase. The oral delivery composition may be able to deliver the uremic toxin-treating enzymes, substantially undigested, to the intestines, where the enzymes can interact with uremic toxins transported to the intestines from the bloodstream. In another aspect, the treatment includes an oral delivery composition comprising a cell able to reduce the concentration of one or more uremic toxins in vivo. In some cases, the cell may be designed to overexpress one, two, or more uremic toxin-treating enzymes, such as urease, uricase or creatininase, for example, by transfecting the cell with a corresponding gene. In some embodiments, a species able to react with or otherwise sequester by-products of the uremic toxin-treating enzyme reactions may be included with the oral delivery composition. For example, if the by-product is ammonium, the species may be a sorbent able to adsorb ammonium, an enzyme able to react with the ammonium, or the like.
Owner:BROWN UNIVERSITY

Visual biosensor device for measuring total concentration of protein

The invention provides a visual biosensor device for measuring total concentration of protein. The device comprises an electrophoresis tube, two four-way glass tubes, two constant current pumps, an anodic electrolyte tank, a cathodic electrolyte tank, an anodic waste liquid tank, a cathodic waste liquid tank, a voltage supply device and a data recording device, wherein the first ports of the two four-way glass tubes are connected with two ends of the electrophoresis tube; one end of each of the two constant current pumps is connected with the second port of each of the two four-way glass tubes; the anodic electrolyte tank and the cathodic electrolyte tank are respectively connected with the other ends of the two constant current pumps; the anodic waste liquid tank and the cathodic waste liquid tank are respectively connected with the third ports of the two four-way glass tubes; the voltage supply device is connected with the fourth ports of the two four-way glass tubes; and the data recording device is positioned on one side of the electrophoresis tube. The total concentration of the protein is measured by recording different relative distances of moving reaction boundaries (MRB) in unit time based on a moving reaction boundary titration (MRBT) technology. Experiments prove that the experiment results are not obviously affected by adding different non-protein nitrogen (NPN), so that interference of the NPN to the protein concentration measurement can be eliminated.
Owner:SHANGHAI JIAO TONG UNIV

Method for simultaneous detection of a plurality of content of non-protein nitrogen-containing compounds in milk by liquid chromatography-tandem mass spectrometry

The present invention discloses a method for simultaneous detection of a plurality of content of non-protein nitrogen-containing compounds in milk by liquid chromatography-tandem mass spectrometry, the method is as follows: adding a protein precipitant into a to-be-tested sample, whirling, centrifuging, adding a degreasing agent into supernatant, whirling, centrifuging, removing a degreasing agent layer, concentrating a lower clearing liquid, dissolving with ammonia acetonitrile, whirling, performing column chromatography with a HLB solid phase extraction column, collecting an eluant, concentrating, dissolving with a liquid mixture of a 0.15% formic acid aqueous solution (containing 5mM of ammonium acetate) and acetonitrile in the volume ratio of 1: 9, and filtering to obtain a to-be-tested solution; preparing a standard mixing working solution series; respectively injecting the standard mixing working solution series and the to-be-tested solution under liquid chromatography-tandem mass spectrometry conditions, making a standard curve, and calculating the content of the non-protein nitrogen-containing compounds in the to-be-tested solution according to the standard curve. The method can be used to simultaneously detect the content of at least two of dicyclanil, dicyandiamide, biuret, cyromazine and melamine, and is high in accuracy and strong in sensitivity.
Owner:THE INSPECTION & QUARANTINE TECH CENT ZHEJIANG ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Method for integrative detection of false packed dairy products in qualitative and quantitative modes

The invention relates to the bioanalysis technology and the food inspection field and provides a method for integrative detection of false packed dairy products in qualitative and quantitative modes. The method is a complete technical scheme which integrates sodium dodecyl sulfate-polyacrylamide gel electrophoresis, liquid chromatography-tandem mass spectrometry and moving reaction boundary electrophoresis titration, and particularly is a complete technical route from qualitative identification on whether false packed dairy products exist, identification of the kind of false protein to quantitative analysis of the ratio of false protein. Compared with existing detection methods, the method has the advantages that whether false packed dairy products exist can be effectively identified, the kind of false protein can be identified in a qualitative mode, and false degree can be measured in a quantitative mode; beside diary product inspection, the method can also be used for inspection of other protein food from qualitative identification to quantitative analysis. Compared with a traditional protein detection method, the method has the advantage that interference of non-protein nitrogen can be avoided.
Owner:SHANGHAI JIAO TONG UNIV

Deer compound protein supplemental feed

The invention relates to compound protein supplement which can improve the growth and development of red deer and sika deer and can enhance the output and quality of pilose antlers. The invention consists of 8 kinds of additive feed, which are non-protein nitrogen (biuret), compound premixed feed containing 1 per cent of trace element and vitamin, zeolite powder, stone powder, magnesium oxide, calcium hydrogen phosphate, sodium bicarbonate and salt, or 4 kinds of protein concentrated feed, which are soybean meal or cotton seed meal, rapeseed meal, mealies alcohol grains (DDGS) and beer grains. The feed contains 85 per cent-90 per cent of dry material, 30-35 per cent of crude protein, 3 per cent-4 per cent of calcium, 1 per cent-2 per cent of phosphate and is rich in amino acid, vitamin, major elements and trace elements. The invention can fully replace 10 per cent-20 per cent of protein feed, reduce the 10 per cent-20 per cent of feed cost, has the advantages of various feed, rich nutrition, good palatability and high use rate. The feed conversion rate is increased by 15 per cent-20 per cent, and the output of pilose antlers is increased by 30 per cent.The deer farms and feed processing factories can mix 25 per cent of compound protein supplement for deer and 75 per cent of concentrated supplement based on the protein and nutrition requirements of deer in different biological periods, so as to directly feed the deer or produce complete grain feed.
Owner:钟立成
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