Visual biosensor device for measuring total concentration of protein
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A biosensor and total concentration technology, applied in the field of analytical chemistry, can solve the problem of not being used to quantitatively measure the total concentration of proteins, and achieve the effect of strong anti-interference ability and time saving.
Active Publication Date: 2012-09-19
SHANGHAI JIAO TONG UNIV
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[0004] Although many biosensors have been used to measure protein concentration or detect NPN impurities such as melamine in food, so far there is no visual biosensor for quantitative determination of total protein concentration and avoiding the interference of non-protein nitrogen (NPN) on protein concentration detection. sensor
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[0020] Below, the present invention will be further described in detail in conjunction with the accompanying drawings. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following the described embodiment.
[0021] Please refer to figure 1 , figure 1 Among them, the visual biosensor device for determining the total protein concentration includes: an electrophoresis tube 5 placed horizontally on an operating platform 3, a gel is placed in the electrophoresis tube 5, and the gel contains polyacrylamide gel, protein solution, Background electrolyte solution and indicator, the composition of polyacrylamide gel includes acrylamide, N-N'-methylenebisacrylamide, ammonium persulfate and tetraethylethylenediamine, the concentration range of polyacrylamide gel is 12.0% -18.0%, the cros...
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Abstract
The invention provides a visual biosensor device for measuring total concentration of protein. The device comprises an electrophoresis tube, two four-way glass tubes, two constant current pumps, an anodic electrolyte tank, a cathodic electrolyte tank, an anodic waste liquid tank, a cathodic waste liquid tank, a voltage supply device and a data recording device, wherein the first ports of the two four-way glass tubes are connected with two ends of the electrophoresis tube; one end of each of the two constant current pumps is connected with the second port of each of the two four-way glass tubes; the anodic electrolyte tank and the cathodic electrolyte tank are respectively connected with the other ends of the two constant current pumps; the anodic waste liquid tank and the cathodic waste liquid tank are respectively connected with the third ports of the two four-way glass tubes; the voltage supply device is connected with the fourth ports of the two four-way glass tubes; and the data recording device is positioned on one side of the electrophoresis tube. The total concentration of the protein is measured by recording different relative distances of moving reaction boundaries (MRB) in unit time based on a moving reaction boundary titration (MRBT) technology. Experiments prove that the experiment results are not obviously affected by adding different non-protein nitrogen (NPN), so that interference of the NPN to the protein concentration measurement can be eliminated.
Description
technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a visual biosensor device for measuring the total protein concentration. Background technique [0002] There are many methods for measuring protein concentration in different biological samples, such as Kjeldahl (Kjeldahl, J.Z., 1883, Anal.Chem., 22, 366-382.), biuret method (Cole, E.R., 1969, Rev. Pure and Appl.Chem., 19, 109-130.), Lowry method (Lowry, O.H., Rosebrough, N.J., Farr, A., Randall, R., 1951, J.Biol.Chem., 193, 265-275.), BCA (Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzan, M.D., Fujiimoto, E.K., Goeke, N.M., Olson, B.J., Klenk, D., 1985, Anal. Biochem. , 150,76-85.), ultraviolet absorption method (Layne, E., 1957, Method. Enzymol., 3, 447-454.; Warburg, O., Christian, W., 1942, Biochem. Z., 310, 384-421 .), and the Coomassie brilliant blue method (Bradford, M., 1976, Anal. Biochem., 72, 248-254.), etc. Each method ...
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