Detection reagent, detection method and detection instrument for proteins

A protein detection and detection reagent technology, which is applied in biological testing, material inspection products, color/spectral characteristic measurement, etc., can solve the problems of inaccurate protein quantitative results, etc., and achieve compact instrument structure, strong anti-interference ability, and accurate and reliable results Effect

Inactive Publication Date: 2010-06-02
NAT INST OF METROLOGY CHINA
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  • Abstract
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  • Application Information

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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a protein detection reagent, method and detection instrument, which can detect the true protein detection in the protein-containing sample, avoid the interference of non-protein nitrogen and the traditional method for different properties and types of proteins The problem of inaccurate quantitative results

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  • Detection reagent, detection method and detection instrument for proteins
  • Detection reagent, detection method and detection instrument for proteins
  • Detection reagent, detection method and detection instrument for proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: As a detection reagent for protein, 2 g of Acid Orange-12 was dissolved in 100 ml of phosphate buffer solution, then 100 ml of glacial acetic acid was added, and finally diluted with 1000 ml of water to obtain a detection reagent.

Embodiment 2

[0041] Example 2: The method of using Acid Orange-12 detection reagent to detect protein, the measurement of protein content in liquid milk: add 0.1~1.5 grams of sample in a 50 ml plastic centrifuge tube, record the weighed quality with a balance, and then add protein to quantify Reagent 40 grams, the balance accurately weighs the quality of the dye reagent and stores it in a special instrument. The shaking solution is fully mixed for 3 minutes and then allowed to stand for 3 minutes. Use a disposable syringe to draw the supernatant and filter it with a needle filter. The filtrate is collected in a special instrument. Put it into a special instrument to measure the value. The measured value of each batch of special reagent without sample is recorded in the instrument. The instrument automatically calculates, gives the protein content and prints the result, and stores the result and the detection device at the same time.

[0042] Table 1 Measurement results of protein content ...

Embodiment 3

[0044] Embodiment 3: the measurement of protein content in milk powder

[0045] Add 0.02 to 0.5 grams of sample into a 50 ml plastic centrifuge tube, record the weighed mass with a balance, then add 40 grams of protein quantitative reagent, accurately weigh the mass of the dye reagent with the balance and store it in a special instrument, oscillate the solution and mix thoroughly for 3 After 3 minutes, let it stand for 3 minutes, use a disposable syringe to draw the supernatant and filter it with a needle filter, collect the filtrate in the sample bottle of the special instrument, and put it into the special instrument to measure the value. The measured value of each batch of special reagent without sample is recorded in the instrument. The instrument automatically calculates, gives the protein content and prints the result, and stores the result and the detection device at the same time.

[0046] Table 2 Measuring results of protein content in three kinds of milk powder samp...

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Abstract

The invention discloses a detection reagent, a detection method and a detection instrument for proteins. The reagent is prepared by the following steps: dissolving 0.1 to 10 weight parts of acid orange-12 into 1 to 500 weight parts of buffer solution of phosphoric acid; adding 1 to 500 weight parts of glacial acetic acid; and finally, diluting with 1 to 5,000 weight parts of water, and oscillating and mixing to obtain the detection reagent for the proteins. Compared with the traditional method, the detection method can effectively detect the content of true proteins, and detect the proteins in a wide range; and the detection accuracy is basically not interfered by non-protein nitrogen-containing compounds such as carbamide and melamine. The instrument has small structure, and is convenient to carry. The detection reagent, the detection method and the detection instrument can be widely applied to quick detection of milk products in the basic level and on the spot.

Description

technical field [0001] The present invention relates to protein detection reagents, detection methods and detection instruments, specifically to provide detection reagents, detection methods and Testing equipment. Background technique [0002] Protein detection is very important to our daily production and life. The reason why milk is mixed with melamine is because the inspection department wants to detect whether the milk is qualified or not, in fact, it is to detect the protein content in it; because protein detection is more difficult, then In the past, the commonly used method was to detect protein according to the N contained in the protein. As a result, there were incidents of counterfeit protein mixed with melamine. It is imperative to provide a scientific and accurate method for protein detection. [0003] Traditional protein detection mainly includes the following methods: Kjeldahl method, Dumas combustion method, biuret method, ultraviolet absorption method, Cooma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/27
Inventor 苏福海全灿张庆和李红梅高德江
Owner NAT INST OF METROLOGY CHINA
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