Detection reagent, detection method and detection instrument for proteins

A protein detection and detection reagent technology, which is applied in biological testing, material inspection products, color/spectral characteristic measurement, etc., can solve the problems of inaccurate protein quantitative results, etc., and achieve compact instrument structure, strong anti-interference ability, and accurate and reliable results Effect
CN101718790AInactive Publication Date: 2010-06-02NAT INST OF METROLOGY CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NAT INST OF METROLOGY CHINA
Publication Date
2010-06-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a detection reagent, a detection method and a detection instrument for proteins. The reagent is prepared by the following steps: dissolving 0.1 to 10 weight parts of acid orange-12 into 1 to 500 weight parts of buffer solution of phosphoric acid; adding 1 to 500 weight parts of glacial acetic acid; and finally, diluting with 1 to 5,000 weight parts of water, and oscillating and mixing to obtain the detection reagent for the proteins. Compared with the traditional method, the detection method can effectively detect the content of true proteins, and detect the proteins in a wide range; and the detection accuracy is basically not interfered by non-protein nitrogen-containing compounds such as carbamide and melamine. The instrument has small structure, and is convenient to carry. The detection reagent, the detection method and the detection instrument can be widely applied to quick detection of milk products in the basic level and on the spot.
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Description

technical field

[0001] The present invention relates to protein detection reagents, detection methods and detection instruments, specifically to provide detection reagents, detection methods and Testing equipment. Background technique

[0002] Protein detection is very important to our daily production and life. The reason why milk is mixed with melamine is because the inspection department wants to detect whether the milk is qualified or not, in fact, it is to detect the protein content in it; because protein detection is more difficult, then In the past, the commonly used method was to detect protein according to the N contained in the protein. As a result, there were incidents of counterfeit protein mixed with melamine. It is imperative to provide a scientific and accurate method for protein detection.

[0003] Traditional protein detection mainly includes the following methods: Kjeldahl method, Dumas combustion method, biuret method, ultraviolet absorption method, Cooma...

Claims

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