Detection reagent, detection method and detection instrument for proteins
A protein detection and detection reagent technology, which is applied in biological testing, material inspection products, color/spectral characteristic measurement, etc., can solve the problems of inaccurate protein quantitative results, etc., and achieve compact instrument structure, strong anti-interference ability, and accurate and reliable results Effect
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Embodiment 1
[0040] Example 1: As a detection reagent for protein, 2 g of Acid Orange-12 was dissolved in 100 ml of phosphate buffer solution, then 100 ml of glacial acetic acid was added, and finally diluted with 1000 ml of water to obtain a detection reagent.
Embodiment 2
[0041] Example 2: The method of using Acid Orange-12 detection reagent to detect protein, the measurement of protein content in liquid milk: add 0.1~1.5 grams of sample in a 50 ml plastic centrifuge tube, record the weighed quality with a balance, and then add protein to quantify Reagent 40 grams, the balance accurately weighs the quality of the dye reagent and stores it in a special instrument. The shaking solution is fully mixed for 3 minutes and then allowed to stand for 3 minutes. Use a disposable syringe to draw the supernatant and filter it with a needle filter. The filtrate is collected in a special instrument. Put it into a special instrument to measure the value. The measured value of each batch of special reagent without sample is recorded in the instrument. The instrument automatically calculates, gives the protein content and prints the result, and stores the result and the detection device at the same time.
[0042] Table 1 Measurement results of protein content ...
Embodiment 3
[0044] Embodiment 3: the measurement of protein content in milk powder
[0045] Add 0.02 to 0.5 grams of sample into a 50 ml plastic centrifuge tube, record the weighed mass with a balance, then add 40 grams of protein quantitative reagent, accurately weigh the mass of the dye reagent with the balance and store it in a special instrument, oscillate the solution and mix thoroughly for 3 After 3 minutes, let it stand for 3 minutes, use a disposable syringe to draw the supernatant and filter it with a needle filter, collect the filtrate in the sample bottle of the special instrument, and put it into the special instrument to measure the value. The measured value of each batch of special reagent without sample is recorded in the instrument. The instrument automatically calculates, gives the protein content and prints the result, and stores the result and the detection device at the same time.
[0046] Table 2 Measuring results of protein content in three kinds of milk powder samp...
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