6results about How to "Avoid nutritional deficiencies" patented technology

The invention relates to a mechanical compression and / or co-culture microfluid device and application thereof, and belongs to the technical field of biomedical science or pharmacology, the microfluid device comprises a culture chamber and a nutrition supply chamber, the culture chamber comprises at least two compartments for accommodating cells / tissues and a porous limiting plate shared with the nutrition supply chamber, the compartments at least comprise a compression compartment for accommodating cells / tissues subjected to mechanical compression and a non-compression compartment which is not subjected to compression strength or is subjected to compression strength far lower than the compression strength of the compression compartment, and the actuating chamber drives the flexible partition plate on the bottom surface of the culture chamber to periodically deform and restore to the original shape after being in contact with the limiting plate; and at least one compartment of the nutrition supply room corresponds to the culture room. The device is free of complex alignment operation, low in manufacturing difficulty, low in cost and particularly suitable for simulating the microenvironment of cartilage tissue, the thin face shape formed by the compression compartment simulates the shape and nutrition supply mode of cartilage, and meanwhile the non-compression compartment can achieve co-culture of multiple kinds of non-compression tissue and compressed cartilage tissue.

The invention discloses an oil sunflower field precise fertilization control system based on an unmanned aerial vehicle hyperspectral technology, and relates to the technical field of fertilization control. The system carries out precise region division on an oil sunflower field by utilizing a GIS technology through fusion of multi-source data such as hyperspectral data, soil humidity and temperature; and accurate fertilization can be carried out in different management areas according to soil characteristics and crop requirements. The differentiated fertilization strategy can reduce excessive fertilization and fertilizer waste and optimize the utilization efficiency of the fertilizer. The crop growth model is established through the deep learning technology, and the system can accurately calculate the required fertilization amount according to the specific growth conditions and historical data of each region, so that the growth conditions of the oil sunflowers are optimized, and the overall yield of crops is improved. The problem that a traditional fertilization system is difficult to effectively deal with soil and environment differences in different areas is solved. Through a hyperspectral technology and multi-source data acquired by a sensor, the system can realize accurate fertilization according to environmental differences in a field.

The invention belongs to the technical field of immune cell culture, and particularly relates to an in-vitro amplification culture method of NK cells. The amplification method comprises the steps of CD16 pretreatment, plasma inactivation, lymphocyte separation and multi-stage fluid infusion. The preparation method comprises the following steps: pretreating CD16 in a T75 culture flask, promoting attachment and activation of NK cells, simulating a natural activation state, improving proliferation efficiency, treating whole blood and inactivating plasma to eliminate immunosuppressive factors, reserving nutritional ingredients, supporting amplification of the NK cells, enriching the NK cells through a lymphocyte separating medium, removing impurities and improving purity, mixing an RPMI-1640 culture medium with a solution A, and culturing for 12-24 hours at a high temperature; oK432 and IL-15 are added, the microenvironment is optimized, growth of the NK cells is remarkably promoted, liquid supplementing operation and bottling and bagging are carried out for multiple times, sufficient space and nutrition are provided, the cells are gradually transferred into a serum-free culture medium, and the cell safety and clinical application potential are improved.

This utility model discloses a feed equalizer for poultry farming, belonging to the technical field of feed equalizers. The feed equalizer for poultry farming includes a feed equalizer box slidably installed in a feed trough, forming a feed accumulation area within the feed equalizer box. The inclined surface of the feed equalizer box and the feed trough form a feed recovery area. Fixed frames are fixedly installed at both ends of the feed equalizer box. It also includes: "L"-shaped guide plates symmetrically installed at both ends of the feed equalizer box, with the outer surface of the guide plates connected to the feed recovery area. Symmetrical interceptor plates are installed on the feed equalizer box, and these interceptor plates are installed within the feed recovery area. Rollers are rotatably installed on the feed equalizer box, and scraper plates are fixedly installed on the rollers. This utility model utilizes the scraper plate in conjunction with the interceptor plates to effectively scrape away some of the old feed located at the edge of the feed trough and collect it in the accumulation area to mix with the new feed. This prevents the old feed from becoming moldy or spoiled due to prolonged lack of consumption, thus avoiding problems such as affecting poultry health and increasing farming costs.

The invention relates to the technical field of blood filters, in particular to a two-channel graded targeted filtering blood filter and a using method.The two-channel graded targeted filtering blood filter comprises a targeted filtering bin, an anticoagulation bin is installed at the upper end of the targeted filtering bin through a second conveying pipeline, and the lower end of the targeted filtering bin is connected with a nutrition bin through a pipeline and a third electromagnetic valve; and an inner shell is installed in the targeted filtering bin, a fixing frame is installed in the inner shell, a first targeted pipe and a second targeted pipe are symmetrically installed in the fixing frame, and a second electromagnetic valve is installed at the upper end of the inner shell. A series of structures are arranged, a double-membrane double-channel filtration system is constructed through series connection design of the first targeting tube polyethersulfone small-aperture membrane and the second targeting tube polyethersulfone large-aperture membrane, small-molecular toxins and medium-macromolecular toxins are sequentially and accurately removed, the contact area is increased through the spiral tubular membrane structure, toxin residues are avoided, and the toxic and side effects are reduced. The blood filtration treatment effect is remarkably improved, and the requirements of patients with different toxin accumulation types are met.

The invention relates to the technical field of biological products, in particular to a preparation method of a culture medium for efficiently culturing mycoplasma gallisepticum at low cost, which comprises the following steps: step 1, dissolving and blending: a, preliminary dissolving: adding NaCl, K2HPO4 and MgSO4. 7H2O into 1000mL of distilled water, heating to 40 DEG C, and slightly stirring until the NaCl, K2HPO4 and MgSO4. 7H2O are completely dissolved to form a basic inorganic salt solution; b, adding a carbon and nitrogen source: adding glucose, soy peptone, tryptone and a yeast extract, and stirring until the materials are completely dissolved; c, pH regulation: regulating the pH value of the solution to 7.4 by using sterile 0.5 M HCl and NaOH; step 2, adding growth factors and nutritional ingredients: a, adding special growth factors: adding L-cysteine and cholesterol under a stirring condition, and continuously stirring until the L-cysteine and the cholesterol are completely dissolved; the cost is low: animal serum and protein raw materials are replaced by using the plant-derived protein hydrolysate, and meanwhile, auxiliary components such as vitamins and growth factors are added, so that the cost of the raw materials is reduced.