Method for integrative detection of false packed dairy products in qualitative and quantitative modes

A dairy and sample technology, applied in the field of bioanalytical technology and food inspection, to avoid interference

Inactive Publication Date: 2015-12-09
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0006] Aiming at the problems existing in the above-mentioned technologies, the present invention provides a method for qualitatively and quantitatively integrated detection of adulterated dairy products, which overcomes the technical problem of incompatibility between traditional technology and cutting-edge analysis technology in the prior art. Sodium sulfonate-polyacrylamide gel electroph

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  • Method for integrative detection of false packed dairy products in qualitative and quantitative modes
  • Method for integrative detection of false packed dairy products in qualitative and quantitative modes

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[0044] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0045] In the technical scheme of the present invention, take the actual sample of doping soybean milk in milk as an example ( figure 1 -A), first carry out SDS-PAGE electrophoresis simultaneously to adulterated sample and unadulterated pure milk ( figure 1 -B), so as to obtain the protein bands of both. Select the protein band that exists in the adulterated sample but does not exist in the pure milk (that is, the difference band between the two) for trypsin digestion, and the sample after enzy...

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Abstract

The invention relates to the bioanalysis technology and the food inspection field and provides a method for integrative detection of false packed dairy products in qualitative and quantitative modes. The method is a complete technical scheme which integrates sodium dodecyl sulfate-polyacrylamide gel electrophoresis, liquid chromatography-tandem mass spectrometry and moving reaction boundary electrophoresis titration, and particularly is a complete technical route from qualitative identification on whether false packed dairy products exist, identification of the kind of false protein to quantitative analysis of the ratio of false protein. Compared with existing detection methods, the method has the advantages that whether false packed dairy products exist can be effectively identified, the kind of false protein can be identified in a qualitative mode, and false degree can be measured in a quantitative mode; beside diary product inspection, the method can also be used for inspection of other protein food from qualitative identification to quantitative analysis. Compared with a traditional protein detection method, the method has the advantage that interference of non-protein nitrogen can be avoided.

Description

technical field [0001] The invention relates to the field of bioanalysis technology and food inspection, and is a method for qualitatively and quantitatively integrated detection of adulterated dairy products, and specifically relates to a complete analysis from qualitatively identifying adulterated food and dairy products to quantitatively determining the degree of adulterated food and dairy products method. Background technique [0002] With the development of social economy, dairy products are favored by people due to their high nutritional value. However, driven by profit, unscrupulous merchants will adulterate dairy products with other substances. The more common adulteration methods are adding inorganic nitrogen such as urea and melamine to dairy products, or adding relatively inferior and low-cost proteins to high-quality dairy products, such as adding soy milk to goat milk and bovine colostrum. Donkey milk is adulterated with milk (PizzanoR, SalimeiE., 2014, J. Agr...

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Application Information

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IPC IPC(8): G01N30/88G01N27/447
Inventor 曹成喜张柳霞曹毅仁肖华樊柳荫
Owner SHANGHAI JIAO TONG UNIV
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