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Method for identifying waste oil by using Caenorhabditis elegans dormancy as biological marker

A technology of Caenorhabditis elegans and biomarkers, applied in biochemical equipment and methods, microbe measurement/inspection, etc., can solve the problems of complex operation, poor accuracy, low sensitivity, etc., and achieve convenient experimental operation and low cost , the effect of low experimental conditions

Inactive Publication Date: 2012-07-18
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of low sensitivity, poor accuracy, complicated operation and high cost in existing waste oil identification methods, and to provide a method for using Caenorhabditis elegans dormancy as a biomarker to identify waste oil

Method used

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Effect test

specific Embodiment approach 1

[0007] Embodiment 1: This embodiment is a method for using Caenorhabditis elegans dormancy as a biomarker to identify waste oil, specifically as follows:

[0008] 1. Prepare medium: use NaCl, agar powder, peptone, 0.8mol / L~1.2mol / L CaCl 2 Aqueous solution, 0.8mol / L~1.2mol / L MgSO 4 Aqueous solution, phosphate buffer solution and distilled water are mixed evenly to obtain a culture medium, sealed and placed in a high-pressure steam sterilizer, and sterilized at 120°C for 15min to 25min, then cooled to 50°C to 60°C, and sterilized Add the edible oil to be tested under the environment, then transfer it to a petri dish, and finally dry it in a sterile environment at a temperature of 20°C for 8h to 12h to obtain a culture medium; Into the concentration of 1 × 10 9 pcs / mL~2×10 9 pcs / mL Escherichia coli OP50 bacterial liquid, then cultured at 37°C for 8h-12h, and then cultured for 46h with the inoculated Caenorhabditis elegans larvae. After 24h of culture, the Caenorhabditis elegan...

specific Embodiment approach 2

[0013] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the phosphate buffer described in step one is made of KH 2 PO 4 , KOH and distilled water, KH in phosphate buffer 2 PO 4 The concentration is 1.36×10 -3 g / mL, the concentration of KOH is 0.18×10 -3 g / mL, and the pH of the phosphate buffer is 6.0. Others are the same as in the first embodiment.

[0014] Adopt following test to verify effect of the present invention:

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Abstract

The invention relates to a method for identifying waste oil by using Caenorhabditis elegans dormancy as a biological marker, and relates to a waste oil identifying method. The method of the invention solves the problems of low sensitivity, poor accuracy, complex operation and high cost existed in the current waste oil identification method. The method comprises the following steps: 1, uniformly mixing NaCl, agar powder, peptone, a CaCl2 aqueous solution, a MgSO4 aqueous solution, a phosphate buffer and distilled water to obtain a nutrient solution, then sterilizing, and drying to obtain a medium; and 2) coating escherichia coli OP50 bacterial liquid in a prepared medium in the step 1), culturing for certain time, and culturing Caenorhabditis elegans larva for 46 hours. The method has the following advantages that 1, effective identification of high refining waste oil with low specific component trace can be realized; and 2, the operation is convenient, the cost is low, the method is easy to train and grasp. The method of the invention is mainly used for identifying the waste oil.

Description

technical field [0001] The invention relates to a waste oil screening method. Background technique [0002] Caenorhabditis elegans (C. elegans for short) is an animal belonging to the phylum Nematode Elegans, and is widely used in the field of scientific research as a typical model organism. Caenorhabditis elegans is transparent, easy to observe the shape and behavior; short life cycle, convenient and cheap to cultivate, easy to store and convenient for experimental operation, the experimental research of Caenorhabditis elegans has formed a series of international standardization and standardized operations. Under unfavorable environmental conditions such as insufficient food, such as lack of important nutritional factors, C. elegans will stop normal growth and development and enter the dormant stage (dauer stage). There are significant differences in behavior, morphology and physiology between the larvae entering the dormant state and the normal developing C. elegans, mani...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 王云彪张雁慧唐占辉
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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