Method for molecular identification of Qinling Mountain illegal trade animal species and primer thereof

Abstract

The invention relates to a method for molecular identification of Qinling Mountain illegal trade animal species and a primer thereof. At present, the conventional method that one primer is for one specie is adopted for the molecular identification of animal species, reaction conditions, reaction times and annealing temperatures of primers are all different, time and experimental materials are wasted, and the case investigating speed is influenced. According to the invention, DNA of a sample is extracted, then PCR amplification is carried out by utilizing a designed primer, PCR products are sequenced and are compared with the obtained sequences of 15 Qinling Mountain illegal trade animals, and the sample with the nearest clustering analysis relationship is namely from the Qinling Mountain illegal trade animals. The method and primer which are provided by the invention can be applied to the molecular identification of 15 Qinling Mountain illegal trade animal species in total such as takin, forest musk deer, goral, serow, elaphodus cephalophus, muntiacus reevesi, black bear, paguma larvata, leopard cat, wild boar, giant panda, snub-nosed monkey, yellow weasel, ferretbadger and badger, identification can be completed by virtue of only one primer, one PCR system and one experiment, the detection flow is simplified, the experimental period is effectively shortened, and the experimental cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of species identification molecules, and in particular relates to a method for molecular identification of illegally traded animal species in the Qinling Mountains and a primer thereof. Background technique [0002] The natural conditions of Shaanxi Province are complex. The Qinling Mountains, as the dividing line between the north and the south of my country's climate, is not only the dividing line between the Oriental and Palaearctic regions of the geographical distribution of animals, but also the gathering place of animals from the north and south of my country and the natural shelter of Quaternary animals. The complex and diverse natural environment has bred the diversity of animal resources in Shaanxi Province, especially the distribution of endemic and rare species of animals, making it the hometown of rare animals such as giant pandas, golden monkeys, crested ibis, and takins. There are currently 604...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for molecular identification of illegally traded animal species in the Qinling Mountains and its primers, which overcomes the lack of a common primer in the identification of illegally traded animal species in the Qinling Mountains in the prior art, which takes a long time to identify, wastes resources, and is not suitable for Problems in performing molecular species identification

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