125 results about "Ailuropoda melanoleuca" patented technology

The invention discloses a method for timely releasing panda pairs to allow natural mating. The method includes: accurately detecting LH (luteinizing hormone) peak and emergence time in female pandas according to true estradiol peak and emergency time thereof in female pandas; arranging normally ovulating female panda individuals for primary natural mating in 16-30 hours after the time of true estradiol peak according to such conditions as true estradiol peak, time intervals between LH peak and true estradiol peak, and whether the female pandas are in heat peak synchronously or not; arranging ovulation-delayed female panda individuals for primary natural mating in 16-30 hours after the time of true estradiol peak; and allowing for single or multiple times of natural mating. The period of primary natural mating is shortened from 48 hours to present 20 hours, success rate of natural mating in panda is increased from the traditional 21.05% to 46.15% finally, and the success rate is more than doubled.

The invention provides a preservation technology for bamboos eaten by pandas, and belongs to the technology field of fresh bamboo preservation. The preservation technology comprises following steps: soaking the bamboo incisions in chitosan coating plastics with a concentration of 0.5 to 2.0%, drying with cold wind then diagonally placing the fresh bamboos with the incisions upward placed at the temperature of 2 to 6 DEG C, steaming the fresh bamboos for 30 to 40 hours with a steaming agent 1-methylcyclopropene with a concentration of 0.5 to 2.0 [mu]L / L, and then storing the fresh bamboos for 15 to 45 days at the refrigeration temperature of 2 to 6 DEG C after the steaming is over. The preservation technology for bamboos eaten by pandas solves the problems that the loss of unique flavors of color, fragrance, taste, and crisp which is caused by the application of chemical preservation technology on bamboos eaten by pandas, and safety hazards existed in conventional chemical antiseptic preservation method, has the advantages of no toxicity, no harm, green, health, strong operability and simple method, and is capable of effectively prolonging the expiration date of the bamboos, and maintaining the original flavor of the bamboos.

The invention discloses a determination method of a giant panda estradiol true peak value. According to the invention, urine samples of female giant pandas at a spring breeding period are collected and are subjected to chemical analysis; conditions such as a time for an estradiol maximal peak or a subsequent estradiol peak to decrease with an amplitude reaching a value above 50%, whether a progesterone value is in an increasing trend during the decreasing period of the estradiol maximal peak or the subsequent estradiol peak, whether the giant pandas show a typical estrous peak behavior, and a ratio of the corresponding progesterone value of the estradiol maximal peak or the subsequent estradiol peak to a progesterone basic value are combined, and the giant panda estradiol true peak value is determined. The method provided by the invention is obtained on the basis of statistical analysis of estradiol variation regularities of 78 oestrus giant pandas which constructs a maximal datum quantity currently, such that the method has high reliability. The method assists in filling up a giant panda research vacancy, and provides a scientific basis for improving captive giant panda artificial insemination conception rate.

The invention relates to a method for molecular identification of Qinling Mountain illegal trade animal species and a primer thereof. At present, the conventional method that one primer is for one specie is adopted for the molecular identification of animal species, reaction conditions, reaction times and annealing temperatures of primers are all different, time and experimental materials are wasted, and the case investigating speed is influenced. According to the invention, DNA of a sample is extracted, then PCR amplification is carried out by utilizing a designed primer, PCR products are sequenced and are compared with the obtained sequences of 15 Qinling Mountain illegal trade animals, and the sample with the nearest clustering analysis relationship is namely from the Qinling Mountain illegal trade animals. The method and primer which are provided by the invention can be applied to the molecular identification of 15 Qinling Mountain illegal trade animal species in total such as takin, forest musk deer, goral, serow, elaphodus cephalophus, muntiacus reevesi, black bear, paguma larvata, leopard cat, wild boar, giant panda, snub-nosed monkey, yellow weasel, ferretbadger and badger, identification can be completed by virtue of only one primer, one PCR system and one experiment, the detection flow is simplified, the experimental period is effectively shortened, and the experimental cost is reduced.

The invention provides a panda wild-training method and belongs to the field of endangered wildlife protection. The panda wild-training method comprises the following steps that at a first training stage, a first training ring is built, panda cows and cubs are bred together, bamboos, steamed corn breads and bamboo shoots are fed to the cows; at a second training stage, a second training ring is enclosed by using a fence, steamed corn breads are provided for the cows, breeding and training are performed for 12-18 months, and the cows and cubs eat bamboos; the cubs are learned to have the ability of recognizing natural enemies; the panda cubs are worn with GPS wireless positioning devices and voice recording devices to be trained in a habitat, image pickup devices are installed in forest land, and the activity range, rules and other behaviors of the cubs are monitored through monitoring devices. The trained panda cubs are cultured to have the field survival ability of obtaining foods by themselves, avoiding natural enemies and the like. The activity situations of pandas are tracked through field monitoring, data collection analysis and the other means to adjust wild-training measures, improve a wild-training effect and improve the ability of adapting to a natural habitat of trained cubs.

The invention discloses a kit used for a paternity test of giant pandas. 68 allelic genes of 11 STR gene loci, obtained by microsatellite study, of the giant pandas are taken as typing standard substances, and parameters of annealing temperature and magnesium ion concentration in a PCR process of the STR gene loci are optimized, wherein the typing standard substances correspond to the allelic genes of the gene loci one by one, so that the microsatellite typing can be performed accurately. The kit of the invention can perform the paternity test and genetic monitoring accurately on species groups of captive giant pandas, complete genealogy, reorganize blood relationship and provide very useful molecular markers for the study in the aspects such as the molecular evolution of the giant pandas, the population migration and the behavioral ecology of wild giant pandas and the like in future, and has very significant theoretical and practical significance on the formulation and implementation of the conservation and breeding plans of rare species.

The invention discloses preparation and application of a giant panda Ascaris schroederi Mcintosh antigen, which belong to the giant panda Ascaris schroederi Mcintosh prevention, treatment and detection field. The method comprises the following steps: a Ascaris schroederi Mcintosh antigen gene primer is designed; total RNA of ascarids undergoes reverse transcription by the RT-PCR method to synthesize cDNA, and then the cDNA is taken as a template for PCR amplification of a target product; the purified target product is connected with a pMD18-T vector and then converted into DH5 alpha competent bacteria; positive recombinant clone is selected through flat screening and culture of the bacteria, and the antigen genes have a Bs-Ag1gene, a Bs-Ag2 gene and a Bs-Ag3 gene after culture and sequencing; and then recombinant plasmids which are accurately sequenced are converted into Escherichia coli BL21 competent cells for mass expression of proteins after construction of the recombinant plasmids, induction expression and purification of the recombinant proteins. After detection and immunologic tests of the product and ELISA detection and analysis of a test animal antibody IgG, the Bs-Ag1gene, the Bs-Ag2 gene and the Bs-Ag3 gene of the giant panda Ascaris schroederi Mcintosh antigen can be taken as candidate genes for preparing genetic engineering vaccines through the sascarids; and the recombinant proteins Bs-Ag1, Bs-Ag2 and Bs-Ag3 have good reactionogenicity and can be used for detecting infection of giant panda Ascaris by the ELISA method.

The invention provides a panda releasing cage, which belongs to the field of an animal transportation device. The panda releasing cage comprises a pull rope, a safety bolt, and a cage body which is provided with a door panel, wherein the cage body is also provided with a pulley block. The door panel is hinged to the cage body by virtue of a spring hinge, the safety bolt is arranged on the free end of the door panel on the cage body, and the door panel can be opened in a long distance by virtue of the safety bolt. One end of the pull rope is connected with the safety bolt, and the other end of the pull rope penetrates through the pulley block. By adopting the panda releasing cage, the door panel can be opened in a long distance so as to release a panda to a field environment which is suitable for living of the panda, the direct contact between the human beings and the to-be-released panda can be avoided, and safety and rapidness for releasing the panda can be realized.

The invention discloses a timely artificial insemination method for a giant panda. The method comprises the steps of: collecting, testing and analyzing urine samples of female giant pandas during oestrus in spring, simultaneously combining with these circumstances such as time that the drop of an estradiol maximal peak value or an estradiol peak value reaches up to over 50%, the condition whether a progesterone value is on the rise and the giant panda generates a typical heat peak behavior in the process of the estradiol dropping from the maximal peak value or subsequent peak value, and the ratio of the progesterone value corresponding to the estradiol maximal peak value or subsequent estradiol peak value to the progesterone basic value, judging a real peak value of the estradiol of the giant panda; then carrying out artificial insemination for the first time within 24-48 hours after the moment when the estradiol real peak value appears, and carrying out artificial insemination for the second time after 6-8 hours after finishing the artificial insemination for the second time, finally obviously improving the pregnancy rate of the giant panda. Compared with the past, the artificial insemination pregnancy rate of the giant panda is improved to 42.11% from 20.41% and is more than doubled.

The invention discloses a new method for preparing frozen semen of a giant panda. Liquid nitrogen (3) is poured into a refrigerator body (5) after upper and lower layers of freezing brackets are put inside the refrigerator body (5); fine tubes (2) containing the frozen semen of grown giant panda are orderly arranged on the upper and lower layers of freezing brackets when the top end face of the upper layer of freezing bracket (1) achieves -100 to -70 DEG C; the fine tubes (2) are moved into the liquid nitrogen (3) inside the refrigerator body (5) after the fine tubes (2) are arranged for 30-90 seconds; and then the fine tubes (2) are arranged inside a liquid nitrogen tank to store for a long period of time after balance. The primary freezing temperature of the giant panda semen is changed into determination of freezing surface temperature range measured by a katathermometer from determination of the distance to the liquid nitrogen surface, so that the novel method is simple, rapid and economic, and ensures that different batches of frozen semen are consistent because of freezing conditions. Thus, stable preparation of high-quality frozen semen is achieved; and progress of protection work of the national treasure panda is greatly promoted.

The present invention discloses preparation and applications of a giant panda follicle-stimulating hormone beta subunit monoclonal antibody, wherein an anti-giant panda FSH[beta] subunit monoclonal antibody hybridoma cell line 09199 is provided, and the preservation number is CCTCC NO: C2016144. According to the present invention, a cell fusion technology is used, the hybridoma cell line secreting the anti-FSH[beat] subunit monoclonal antibody is established, the cell line culture supernatant is collected and purified, the highly-specific FSH[beta] subunit monoclonal antibody is obtained, application of the highly-specific FSH[beta] subunit monoclonal antibody in Western Blot protein detection is established to provide information for FSH protein location and tissue expression, and the deep researches on the ligand, the antagonist and the anti-antagonist of the FSH receptor are performed with the highly-specific FSH[beta] subunit monoclonal antibody so as to create the conditions for the founding of the specific ligand, the antagonist and the anti-antagonist; and the new data and the new idea are provided for the further exploration of the FSH secretion, the further regulation of the genes, and the revealing of the reproductive physiological laws.

The invention discloses a giant panda wild releasing training ground. The giant panda wild releasing training ground is characterized in that sites with terrain height difference are selected to be used as training sites, and food release regions, drinking water sources, nests, fences and monitoring equipment are arranged inside the training sites; the training sites are divided into transitional training grounds and wild training grounds; the fences are arranged on the peripheries of the training sites. The giant panda wild releasing training ground has the advantages that requirements on giant panda wild releasing transitional training can be met by means of reasonable planning; standardized hardware conditions can be provided for scientific research on captive giant panda wild releasing, and systematic scientific bases can be provided for gradually implementing giant panda wild releasing.

The invention discloses a quick panda progesterone concentration determination method. On the premise of no difference between detection results of urine samples, three hours for whole determination process of panda progesterone concentration is shortened to 1.5 hours by changing 2-hour incubation at conventional room temperature into 0.5-hour incubation at 37 DEG C, determination time is shortened by 50%, and precious time is earned for natural mating and artificial insemination in good time.

The invention discloses a giant panda semen freezing and preserving fluid and a preparation method thereof. The giant panda semen freezing and preserving fluid disclosed by the invention is prepared from glucose, levulose, trisodium citrate, yolk, glycerin and distilled water. The preparation method comprises the following steps of: firstly, dissolving solid ingredients; then, adding liquid ingredients; and fully mixing uniformly to obtain a finished product. By using the freezing and preserving fluid disclosed by the invention for freezing and preserving giant panda semen, the activity of the unfrozen semen accords with the requirements of artificial fertilization, the average activity is greater than or equal to 58%, and the maximum activity is 65%. Compared with the American TEST preserving fluid, the giant panda semen freezing and preserving fluid has the advantages that the required reagents and raw materials are convenient to acquire and are low in price, the cost for preparing 100mL of the freezing and preserving fluid is about 50 yuan, the preparation method is simple and convenient, the fluid can be prepared and then used on the site, the instant preservation of the semen can be realized, and the frozen semen can be preserved for more than 50 years in liquid nitrogen. The giant panda semen freezing and preserving fluid provides the semen which accords with the requirements of artificial fertilization of giant pandas, greatly lowers the cost for artificially breeding giant pandas and preserving semen for a long time, and has important significance for improving the reproductive performance of giant pandas and enlarging the population quantity of giant pandas.

The invention discloses a manual intervening domestication method for panda feralization and reintroduction. The method comprises the steps of (1) emotional cultivation: an individual panda to be released is separated from the cow, and a releasing worker contacts with the individual panda to be released alone; (2) action training: the releasing worker trains actions of the individual panda to be released, and training terms include the activity amount, the tree climbing ability, independence, and alert and investigation ability for the outside; (3) outdoor environment adaption: the releasing worker trains outdoor survival abilities of the individual panda to be released, and the outdoor survival abilities include food and water source searching, hodling away from natural enemy and the hiding ability. By adding manual intervention into a panda feralization training process, the manual intervening domestication method is beneficial to emotion building between the individual panda to be released and the releasing worker, and brings convenience for monitoring after releasing; since the panda feralization and reintroduction are guided manually, the individual panda to be released can adapt the outdoor environment gradually, the adaptability of the panda to the outdoor environment is improved, and therefore the success rate of feralization and reintroduction is guaranteed.

The invention discloses a preparation method of a recombinant panda IL-2 immune adjuvant. The recombinant panda IL-2 immune adjuvant is a recombinant panda IL-2 (gpIL-2) immune adjuvant, which has a prominent effect and a convenient extraction process, can be directly applied to production and clinical application, improves the immune effect of canine distemper vaccine strain and other conventional vaccines, controls the occurrence and epidemic of diseases, and protects the health of panda. Canine distemper is usually known as "the fatal infectious disease", has become the first fulminating infectious disease that threatens the species number and life safety of pandas, and badly affects the healthy development of pandas. The recombinant panda IL-2 (gpIL-2) immune adjuvant can prominently strengthen the immune effect of canine distemper vaccine strain, and has an important meaning on preventing and treating of canine distemper.

The invention discloses a giant panda lutropin beta subunit monoclonal antibody preparation method and an application thereof, which belong to the technical field of biology. A preservation number ofan anti-giant panda lutropin beta subunit monoclonal antibody hybridomas cell strain 14081-9 is CCTCC NO:C2018128. A cell fusion technology is used for establishing the hybridomas cell strain for secreting the anti-giant panda lutropin beta subunit monoclonal antibody, the cell strains are collected and purified to culture a supernatant, and the high-specificity lutropin beta subunit monoclonal antibody is obtained, the established high-specificity lutropin beta subunit monoclonal antibody is used for Western Blot protein detection, LH protein positioning and tissue expression information, anddeep researches on LH acceptor ligand, an antagonist, and an anti-antagonist by the LH beta submit high-specificity monoclonal antibody can be carried out, and conditions can be created for discoveryof the specific ligand, the antagonist, and the anti-antagonist. The method provides novel data for discussing LH secretion and gene further regulation and control, reveals a reproduction physiological law, reveals reproduction physiological law in giant panda, and grasps giant panda oestrus ovulation and pregnancy rules.

The invention provides a panda releasing cage, which belongs to the field of an animal transportation device. The panda releasing cage comprises a pull rope, a safety bolt, and a cage body which is provided with a door panel, wherein the cage body is also provided with a pulley block. The door panel is hinged to the cage body by virtue of a spring hinge, the safety bolt is arranged on the free end of the door panel on the cage body, and the door panel can be opened in a long distance by virtue of the safety bolt. One end of the pull rope is connected with the safety bolt, and the other end of the pull rope penetrates through the pulley block. By adopting the panda releasing cage, the door panel can be opened in a long distance so as to release a panda to a field environment which is suitable for living of the panda, the direct contact between the human beings and the to-be-released panda can be avoided, and safety and rapidness for releasing the panda can be realized.

The invention discloses a 38kDa antigen for Baylisascaris schroederi of giant pandas, a preparation method for the antigen and application of the antigen. A gene of the antigen is a Bsc38 gene; a nucleotide sequence of the gene is shown in a table 1; the Bsc38 gene encodes a nucleotide sequence of mature peptide; and a corresponding amino acid sequence is shown in a table 2. A candidate antigen which is used for preparing a genetic engineering vaccine for preventing and controlling Baylisascaris schroederi disease of the giant pandas is identified and screened to solve the problems that Baylisascaris schroederi disease of the giant pandas is prepared and controlled mainly by medicines at present, medicine-resistant worm strains are caused by the frequent use of medicines, the pollution of food chains and environments is caused by chemical medicines, and the like. Compared with the prior art, the invention has the advantages that the antigen can reduce the medicine-resistant worm strains and the pollution of the medicines to the food chains and the environments in the medicine control process of Baylisascaris schroederi disease of the giant pandas, and can reduce the repeated infection of pandas in natural environments. The invention discloses a molecular immunologic mechanism after larvae of the Baylisascaris schroederi are infected with a host for the first time at home and abroad.

The invention discloses a panda transportation cage which comprises a transportation cage body. The transportation cage body comprises a cuboid frame and a fence bar, and an opening and closing door is arranged at the front end of the transportation cage body. The panda transportation cage is characterized in that the left and right sides, the upper end and the back end of the transportation cageare provided with buffer battens, and buffer batten raw materials include panda feces and saw dust; and the panda transportation cage has the flavor of the panda feces, a panda can adapt to the transportation cage as soon as possible, a stress reaction of the panda is better reduced, and the health of the panda is facilitated.

The invention discloses a nest for a panda running-wild reintroduction training ground. The nest mainly comprises a tree cavern and a stone cavern. The tree cavern is made from fir wood; the tree cavern is provided with a tree cavern opening and a tree cavern ventilation opening; floors laid from wood are arranged at the bottom in the tree cavern; the stone cavern is made from stone and cement; the stone cavern is provided with a stone cavern opening and a stone cavern ventilation opening; floors laid from wood are arranged at the bottom in the stone cavern. The nest for the panda running-wild reintroduction training ground has the advantages that an artificial nest is constructed in the training ground, and the panda running-wild reintroduction basic skill can be met; meanwhile, normalized hardware conditions are provided for scientific research of captive giant panda running-wild reintroduction, and the systematic scientific basis is provided for gradually achieving panda running-wild reintroduction.

The invention discloses a wild returning, releasing and feeding method for captive ailuropoda melanoleuca. The wild returning, releasing and feeding method disclosed by the invention chooses different training modes according to different month ages of released individuals. The training modes include full female ailuropoda melanoleuca, female ailuropoda melanoleuca + beginning artificial training intervention and artificial training. According to the different month ages of the released individuals, different feeding modes are chosen, and the feeding modes include full breast milk, female ailuropoda melanoleuca milk + artificially supplementing milk, breaking ailuropoda melanoleuca milk + artificially supplementing milk, gradually reducing artificially supplementing milk and stopping artificially supplementing milk; according to the different month ages of the released individuals, different feeding sites are chosen, and the feeding sites mainly include a captive place, an indoor and outdoor sports field of the captive place, a first half wild returning training ground, a second half wild returning training ground and a habitat training place. The wild returning, releasing and feeding method disclosed by the invention has the beneficial effect of preventing wild returned and released individuals from learning self captive habits of female ailuropoda melanoleuca and accordingly improving a releasing success rate of the captive ailuropoda melanoleuca.

The invention discloses a giant panda ribosomal subunit gene RPL23a recombinant protein and application thereof in preparing anti-cancer drugs. The amino acid sequence of the recombinant protein is defined by SEQ ID NO: 7. The inhibitory effect of giant panda ribosomal subunit gene RPL23a recombinant protein on growth of Hep-2 cells is observed by acting the giant panda ribosomal subunit gene RPL23a recombinant protein on the Hep-2 cells, which reveals the anticancer function of the giant panda ribosomal subunit gene RPL23a recombinant protein and provides scientific basis and resources for developing anti-cancer protein drugs and studying on anti-cancer mechanism.

The invention discloses an audio-based prediction method and system for natural mating results of captive pandas, and the method comprises the steps: collecting sound data of captive pandas during natural mating, and obtaining original audio data; preprocessing the collected original audio data to obtain preprocessed audio clips; extracting Mel-frequency cepstral coefficient characteristics of thepreprocessed audio clips; utilizing a neural network model to judge the extracted Mel-frequency cepstral coefficient characteristics, and analyzing and predicting the prediction probability of a mating result predicted by each frame in the audio clip; and counting prediction probabilities of a plurality of audio frames in the audio clips input into the neural network model, and obtaining prediction judgment of a panda mating result according to statistical data. According to the method, the sound of the panda during mating is analyzed, so that the natural mating result of the panda is automatically predicted. Panda breeding researchers are assisted in achieving optimal natural mating pairing selection in the short optimal mating period of pandas.

The invention discloses a method for rapid detection of a giant panda estradiol concentration value. Under the premise of no urine sample detection result difference, the tradition way of incubation at room temperature for 2h is changed to incubation for 0.5h at 37DEG C, so that the total detection process of the giant panda estradiol concentration value is shortened from the original 5h to 2h, and the detection time is shortened by 60%, thus winning precious time for timely natural mating and artificial insemination.

The invention discloses a PCR (Polymerase Chain Reaction) method for detecting Baylisascaris schoederi eggs in panda excrement sample, which comprises the following four steps: design of a forward primer and a reverse primer, extraction of egg DNA, PCR amplification and result examination. With the method, the egg is treated before DNA extraction, general grinding is replaced by adopting a manner of repeated freeze thawing of boiling water of 100 DEG C and liquid nitrogen, the fracture of the shell of egg protein can be observed under a microscope so that the DNA can be exposed to be convenient for extraction, and the loss in DNA extraction can be reduced as much as possible. Simultaneously, the invention provides two PCR amplification primers. Compared with the prior art, with the method, the detection accuracy and the sensitivity are greatly improved, the relevance ratio is higher than that of a conventional method (a floating method) by 32-47.83 percent, and the situation of ascariasis infection of panda can be judged more accurately; therefore, the method has significant meaning on diagnosing and preventing and controlling ascariasis of the panda.

The invention provides a nutritive steamed corn-bread for captive alluropoda melanoleuca. According to the invention, the steamed corn-bread is prepared from the main raw materials of bamboo, tofu skin, alfalfa, carrot, cabbage, apple, cucumber, corn flour, egg yolk powder, tea-seed oil, and fresh bamboo juice. The steamed corn-bread has the characteristics of rich nutrients and balanced nutrient contents. The steamed corn-bread is suitable to be used as an alluropoda melanoleuca basic food for satisfying everyday energy consumption and nutrition requirement of alluropoda melanoleuca. Also, functional steamed corn-breads rich in different nutrients can be prepared by adding different proportions of fiber components, protein components or trace components on the basis of the main raw materials, such that body requirements of different alluropoda melanoleuca individuals and different stages can be satisfied. According to the invention, bamboo juice which is a residue of bamboo fiber processing pre-treatment is subjected to standing, filtering, and sterilizing treatments, such that a bamboo liquid is obtained. The bamboo liquid is used for replacing water in preparing the nutritive steamed corn-bread. Therefore, the prepared steamed corn-bread has a rich bamboo flavor, and alluropoda melanoleuca appetite can be promoted.

The invention belongs to the field of release training for pandas and particularly relates to an adaptation site for use before return of pandas to wild state. In order to solve the problem that the prior art has no regularized adaption sites for use before the return of pandas to the wild state, the invention discloses the adaptation site for use before return of pandas to the wild state. According to the technical scheme, the adaptation site has an altitudinal gradient ranging from 2100 m to 3000 m; the inside of the adaptation site has habitat types for survival of wild pandas; the habitattypes for the survival of wild pandas include naturally grown edible bamboos for panda, natural mobile water sources, and trees and shrubs; a fence (5) is arranged outside the adaptation site. The adaptation site is suitable for release training of pandas.

The invention discloses a suitable habitat restoration method for giant pandas. In order to overcome the shortcoming that no giant panda suitable habitat restoration method by using an artificial coniferous forest environment exists in the prior art, the method disclosed in the invention comprises steps: firstly, the characteristic data of plant communities in the artificial coniferous forest areinvestigated; secondly, according to the characteristic data of the plant communities, a habitat restoration site under a canopy and a forest gap habitat restoration site are selected; thirdly, a forestland in the restoration site is trimmed again; and finally, trees are planted in the habitat restoration site under the canopy and the forest gap habitat restoration site respectively according to afirst mode and a second mode, wherein the first mode is mixed planting of giant panda's staple food bamboo and evergreen trees, and the second mode is mixed planting of giant panda's staple food bamboo, evergreen trees and deciduous trees. The obstacles that can not be overcome in the prior art can be broken through, a method of restoring the suitable habitat for the giant pandas by using the artificial coniferous forest environment within the habitat of the giant pandas is provided, and the technical problem which is eager to be solved by people in the field but is not successfully solved can be solved.

The invention discloses a panda oestrone peak time prediction method and application thereof. The panda oestrone peak time prediction method comprises the steps of: when a female panda enters an oestrus season and the vulva color becomes red, collecting panda urine, and measuring the content of creatinine, oestrone and progesterone in the panda urine respectively; when the creatinine content is greater than 0.2 mg / mL, calculating the oestrone content after creatinine correction; when the oestrone content after creatinine correction is greater than 20 ng / mg Cr for the first time, indicating panda oestrus starting, recording a panda urine collection time point as oestrus starting time, extending backwards for 4 days from the starting time, performing E / P value calculation on panda urine of which the creatinine content is greater than 0.2 mg / mL and the progesterone content is greater than 2.5 ng / mL, and determining a target E / P value according to the E / P value; and determining appearancetime of a panda oestrone peak value according to the panda urine collection time point corresponding to the target E / P value. The panda oestrone peak time prediction method is high in prediction timeperiod accuracy degree, good in repeatability, simple to operate and small in workload.