Giant panda semen freezing and preserving fluid and preparation method thereof

Abstract

The invention discloses a giant panda semen freezing and preserving fluid and a preparation method thereof. The giant panda semen freezing and preserving fluid disclosed by the invention is prepared from glucose, levulose, trisodium citrate, yolk, glycerin and distilled water. The preparation method comprises the following steps of: firstly, dissolving solid ingredients; then, adding liquid ingredients; and fully mixing uniformly to obtain a finished product. By using the freezing and preserving fluid disclosed by the invention for freezing and preserving giant panda semen, the activity of the unfrozen semen accords with the requirements of artificial fertilization, the average activity is greater than or equal to 58%, and the maximum activity is 65%. Compared with the American TEST preserving fluid, the giant panda semen freezing and preserving fluid has the advantages that the required reagents and raw materials are convenient to acquire and are low in price, the cost for preparing 100mL of the freezing and preserving fluid is about 50 yuan, the preparation method is simple and convenient, the fluid can be prepared and then used on the site, the instant preservation of the semen can be realized, and the frozen semen can be preserved for more than 50 years in liquid nitrogen. The giant panda semen freezing and preserving fluid provides the semen which accords with the requirements of artificial fertilization of giant pandas, greatly lowers the cost for artificially breeding giant pandas and preserving semen for a long time, and has important significance for improving the reproductive performance of giant pandas and enlarging the population quantity of giant pandas.

Description

technical field [0001] The invention belongs to the technical field of biological material preservation, and in particular relates to a giant panda semen cryopreservation solution and a preparation method thereof. Background technique [0002] The giant panda is a unique species in my country, and it is an endangered rare animal in the world. As of the third national giant panda population survey completed in 2004, although the wild population of giant pandas has risen from more than 1,100 in the 1980s to more than 1,590, But still not out of the endangered situation. After years of hard work, the level of captive breeding of giant pandas in my country has been greatly improved. By the end of 2008, the number of captive populations had reached 268. However, how to avoid inbreeding has become a problem that giant panda captive breeding must face. Through the research on the preservation technology of giant panda semen, the semen of male individuals with good genetic performa...

AI Technical Summary

Problems solved by technology

[0005] The giant panda semen is cryopreserved with TEST preservation solution. Although the vitality of the thawed sperm meets the requirements of artificial insemination, there are the following problems: ①The formula is not disclosed, there is no domestic production, it is inconvenient to purchase, and the price is expensive. (5ml x 20 bottles) RMB 7,000; ②Short shelf life, less than 1 year at -10°C

Since the giant panda is in estrus seasonally, once a year, and the estrus period lasts for a short time, when semen is collected every year for cryopreservation, it is necessary to order and purchase TEST preservation solution in advance, and the remaining TEST preservation solution of the current year cannot be used in the next year

In addition, cryopreservation solution is a basic reagent in the artificial breeding of giant pandas, which is in great demand, and it is also a nutritional reagent containing protein. It is imported and stored for a long time, which will affect the instant cryopreservation of semen and also affect the work. bring inconvenience