Egg worm grass and cultivation method thereof

Abstract

The invention provides egg cordyceps sinensis and a cultivation method thereof. The cultivation method comprises the following steps: S1, selecting fresh eggs; s2, forming small holes in the eggshell, stirring yolk through the small holes, and pumping out a certain amount of egg liquid; s3, injecting a sugar aqueous solution into the eggs from which the egg liquid is extracted, and then shaking the eggs to mix the egg liquid with the sterilized sugar aqueous solution; s4, inoculating cordyceps sinensis liquid strains into the mixed eggs, uniformly shaking, and sealing the small holes; s5, the eggs inoculated with the cordyceps sinensis liquid strains are placed in an environment with the temperature being 22-25 DEG C and the humidity being 40-60% to be cultured for 10-30 days; and S6, removing part of eggshells from the cultured eggs, putting the eggs into a disinfected transparent container, fixing, sealing a cover, putting in an environment with the temperature of 20-25 DEG C and the humidity of 65-85%, and culturing under illumination until the eggs grow out and are mature to obtain the egg cordyceps sinensis. The method has the advantages of wide and cheap raw material sources, simplicity in operation, low requirements on a culture environment, short production period and the like, and has good economic benefits.

Description

Technical Field

[0001] The invention relates to the food processing field of agricultural technology, and in particular to a cordyceps sinensis and a cultivation method thereof. Background Art

[0002] Like Cordyceps sinensis, Cordyceps sinensis and Cordyceps militaris, Cordyceps sinensis is a wonderful creature with both "animal" and "plant" morphological characteristics. It is the insect larvae or pupae infected and parasitized by Cordyceps sinensis, which absorbs the nutrients of the insect body and transforms them into mycelium, and finally grows fruiting bodies from the larvae's head or pupa. As a traditional Chinese medicine, Cordyceps sinensis has a wide range of effects and functions, such as regulating immunity, replenishing the lungs and qi, relieving cough and asthma, replenishing the kidneys and essence, resisting fatigue, improving sleep, and protecting the liver. At present, wild Cordyceps sinensis is becoming less and less, and the price is getting higher and higher. Wild resources are limited and difficult to collect, which makes it difficult to meet market requirements, making it imperative to cultivate Cordyceps sinensis artificially. In order to cultivate Cordyceps sinensis on a large scale, it is necessary to breed insects, but breeding insects is difficult and unsafe. Using artificially prepared nutrient medium instead of insects can cultivate Cordyceps sinensis fruiting bodies, but due to the large loss of nutrients after high-pressure sterilization, its efficacy is far inferior to that of wild Cordyceps sinensis. Summary of the invention

[0003] The inventor has always wanted to use eggs to replace insect larvae or pupae to cultivate Cordyceps. Eggs are high-quality nutritious proteins with nutritional value superior to insect larvae or pupae. Humans eat them every day. They are not only safe but also in large quantities. The inventor applied for patent number ZL200310112611.7 for "Cordyceps eggs and production method thereof" on December 12, 2003. After more than 20 years of continuous improvement, new technical breakthroughs have been made.

[0004] Through continuous research, the inventor has achieved artificial cultivation of various cordyceps, mastered the separation, purification and cultivation technology of cordyceps fungi, and continuously domesticated the cordyceps fungi to enable them to grow in eggs. By controlling environmental factors such as temperature, humidity, and air cleanliness, the inventor ensured that the fruiting bodies of cordyceps can be successfully grown in eggs. Thus, egg cordyceps products with specific medicinal value were developed, among which egg cicada flower cordyceps and egg pupa cordyceps have better nutrition and efficacy than wild cicada flower and pupa cordyceps.

[0005] Specifically, the embodiment of the present invention provides a method for cultivating egg cordyceps, which comprises: S1, choose fresh eggs; the eggs must be cleaned and disinfected S2, under sterile conditions, a small hole is punched on the top of the eggshell with a special puncher, and the yolk membrane is broken through the small hole with a tool, and the yolk and egg white are mixed evenly by shaking, and then a certain amount of egg liquid is extracted with a syringe; S3, injects 5-8ml sterilized sugar aqueous solution (glucose, sucrose, fructose etc.) into the egg from which egg liquid has been extracted, and shakes the egg then so that the egg liquid is mixed with the sugar aqueous solution; S4, inserting liquid Cordyceps spawn into the mixed eggs, and then shaking them evenly, sealing the small holes, and placing them vertically in the egg tray with the small holes facing upwards; S5, placing the eggs inoculated with Cordyceps liquid spawn in a dark environment at a temperature of 22-25 degrees and a humidity of 40%-60% for 10-30 days; S6. When the egg liquid cannot be shaken, it means the mycelium has grown well. Remove part of the eggshell from the top of the cultured egg, put it into a sterilized transparent container, fix it and seal it, place it in an environment with a temperature of 20-25 degrees and a humidity of 65%-85%, and culture it under light until fruiting bodies grow out. Generally, it will take 20-30 days for it to mature, and then you can get egg Cordyceps.

[0006] Preferably, in step S1, the fresh eggs are chicken eggs, duck eggs, goose eggs, quail eggs or pigeon eggs, preferably chicken eggs.

[0007] Preferably, after step S1 and before step S2, the method further includes: The eggshells of the eggs were sterilized with 75% alcohol and placed in a clean bench. The tools placed in the clean bench, such as syringes and punchers, were all sterilized.

[0008] Preferably, in step S2, the small hole is formed on the large end of the egg; the volume of the extracted egg liquid is 15%-30% of the total volume of the egg liquid.

[0009] Preferably, in step S3, the concentration of the sterilized aqueous sugar solution is 30%-50%, and the volume is 30%-50% of the volume of the extracted egg liquid.

[0010] Preferably, the liquid Cordyceps spawn is Cordyceps militaris or Cordyceps militaris liquid spawn, and the volume is 3%-5% of the total volume of the egg liquid.

[0011] Preferably, in step S6, the egg is shaken. If no liquid flows in the egg, it means that the egg is a cultured egg; if liquid flows in the egg, it means that the egg is an uncultured egg.

[0012] Preferably, in step S6, the removed eggshell portion is the eggshell near the small hole. When the egg is fixed, an egg tray is placed in a transparent container, and the egg is placed in the egg tray with the end with the eggshell removed facing upward.

[0013] Preferably, in step S6, the light intensity of the culture is 200-1000LX, the grass grows after 7-15 days of culture, and matures after 20-40 days of culture. When the fruiting body grows spores, it indicates that it is mature.

[0014] The embodiment of the present invention also provides a Cordyceps sinensis, which is cultivated by the cultivation method as described above.

[0015] Compared with the existing cultivation method, the present invention has the following advantages: (1) The raw material is fresh eggs, which are widely available and cheap. Various fresh eggs are widely available.

[0016] (2) The operation is simple and has low requirements for the culture environment, and high-temperature sterilization is not required.

[0017] (3) The production cycle is short. It only takes 7-30 days from inoculation to the full growth of mycelium in the egg, and only 20-40 days from shelling to the growth of fruiting bodies, which has good economic benefits.

[0018] In addition, the success of artificial cultivation of egg Cordyceps militaris and egg Cordyceps militaris has increased production and reduced prices, making it a product that ordinary people can afford. This not only meets people's demand for health products, but also provides possibilities for the prevention and control of chronic diseases. Modern research shows that Cordyceps militaris and egg Cordyceps militaris have the effects of improving kidney function, helping sleep, lowering blood pressure, lowering blood sugar, enhancing immunity, anti-tumor, delaying aging, etc., and have unique effects on a variety of chronic diseases. Therefore, egg Cordyceps militaris and egg Cordyceps militaris not only have important value in health benefits, but also show great potential in economic and social benefits. DETAILED DESCRIPTION

[0019] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Example 1 1. Choose several fresh eggs. Each egg should be laid no more than one week ago and make sure that the eggs are not damaged.

[0021] 2. Disinfect the eggshells with 75% alcohol and place them in a clean bench for preparation.

[0022] 3. Use a sterilized egg puncher in a clean bench to make a pinhole in the big end of the egg. Use a sterile needle-like tool to enter the egg to break the yolk membrane, and shake to mix the yolk and egg white evenly.

[0023] 4. Use a sterilized needle of 15ml or more to draw out 12-15ml of egg liquid from the hole of the egg. The egg liquid of an egg is usually 50-55ml, and the drawn egg liquid accounts for about 20-25%.

[0024] 5. Inject 5 ml of 50% sterilized glucose aqueous solution into the eggs from which the egg liquid has been extracted, and then shake the eggs to mix the egg liquid and glucose evenly.

[0025] 6. Add 2 ml of cicada fungus liquid culture, then continue to shake evenly and stick the pinhole.

[0026] 7. Place the eggs with the cicada fungus liquid culture in the egg tray with the pinhole facing upwards, and then culture them in a room with a temperature of 22-25 degrees and a humidity of 40-60% for 10-15 days.

[0027] 8. After 15 days, shake each egg. If no liquid flows inside, disinfect the egg. If liquid flows inside, it may be contaminated and the egg should be discarded.

[0028] 9. Disinfect the outside of the egg with 75% alcohol, then use an egg opener to remove a part of the eggshell with the pinhole head, put it into the egg tray in a sterilized transparent plastic box with the opening facing up, cover the transparent plastic box with the lid, and place it in a room with a temperature of 20-25 degrees and a humidity of 65-85%, and cultivate it under light intensity of 300-600LX.

[0029] 10. Generally, the Cordyceps sinensis can emerge from the base after 7-10 days of cultivation, and the fruiting body will mature after another 25-30 days of cultivation.

[0030] 11. When the fruiting body grows spores, it means it is mature and can be harvested. After harvesting, it can be eaten fresh or dried and made into powder or various products.

[0031] Example 2 1. Choose several fresh duck eggs. The laying date of each egg should not exceed 1 week. Make sure the eggs are not damaged.

[0032] 2. Disinfect the duck egg shells with 75% alcohol and place them on a clean workbench for preparation.

[0033] 3. Use a sterilized duck egg puncher in a clean bench to make a pinhole in the big end of the duck egg. Use a sterile needle-like tool to enter the egg to break the yolk membrane, and shake to mix the yolk and egg white evenly.

[0034] 4. Use a sterilized injection needle to extract 15-20ml of duck egg liquid from the egg hole, and the extracted egg liquid accounts for 20-25%.

[0035] 5. Inject 6 ml of 40% sterilized sucrose aqueous solution into the duck eggs from which the egg liquid has been extracted, and then shake the duck eggs to mix the duck egg liquid and glucose evenly.

[0036] 6. Add 3 ml of cicada fungus liquid culture, then continue to shake it evenly and stick the pinholes.

[0037] 7. Place the duck eggs with the cicada fungus liquid culture in the egg tray with the pinhole facing upwards, and then culture them in a room with a temperature of 22-25 degrees and a humidity of 40-60% for 10-20 days.

[0038] 8. After 20 days, shake each egg. If no liquid flows inside, disinfect the egg. If liquid flows inside, it may be contaminated and the egg should be discarded.

[0039] 9. Disinfect the outside of the egg with 75% alcohol, then use an egg opener to remove a part of the eggshell with the pinhole head, put it into the egg tray in a sterilized transparent plastic box with the opening facing up, cover the transparent plastic box with the lid, and place it in a room with a temperature of 20-25 degrees and a humidity of 65-85%, and cultivate it under light intensity of 500-1000LX.

[0040] 10. Generally, the Cordyceps sinensis can emerge from the base after 7-10 days of cultivation, and the fruiting body will mature after another 25-30 days of cultivation.

[0041] 11. When the fruiting body grows spores, it means it is mature and can be harvested. After harvesting, it can be eaten fresh or dried and made into powder or various products.

[0042] Example 3 1. Choose several fresh eggs. Each egg should be laid no more than one week ago and make sure that the eggs are not damaged.

[0043] 2. Disinfect the eggshells with 75% alcohol and place them in a clean bench for preparation.

[0044] 3. Use a sterilized egg puncher in a clean bench to make a pinhole in the big end of the egg. Use a sterile needle-like tool to enter the egg to break the yolk membrane, and shake to mix the yolk and egg white evenly.

[0045] 4. Use a sterilized injection needle of more than 15ml to extract 12-15ml of egg liquid from the hole of the egg. The egg liquid of an egg is usually 50-55ml, and the extracted egg liquid accounts for 20-25%.

[0046] 5. Inject 5 ml of 50% sterilized glucose aqueous solution into the eggs from which the egg liquid has been extracted, and then shake the eggs to mix the egg liquid and glucose evenly.

[0047] 6. Add 2 ml of Cordyceps militaris liquid culture, then continue to shake evenly and stick the pinholes.

[0048] 7. Place the eggs with the Cordyceps militaris liquid spawn in the egg tray with the pinhole facing upwards, and then culture them in a room with a temperature of 20-25 degrees and a humidity of 40-60% for 15-25 days.

[0049] 8. After 25 days, shake each egg. If no liquid flows inside, disinfect the egg. If liquid flows inside, it may be contaminated and the egg should be discarded.

[0050] 9. Disinfect the outside of the egg with 75% alcohol, then use an egg opener to remove a part of the eggshell with the pinhole head, put it into the egg tray in a sterilized transparent plastic box with the opening facing up, cover the transparent plastic box with the lid, and place it in a room with a temperature of 23-25 ​​degrees and a humidity of 75-85%, and cultivate it under light intensity of 300-600LX.

[0051] 10. Generally, the Cordyceps pupae can emerge from the base after 8-15 days of cultivation, and the fruiting body will mature after another 25-35 days of cultivation.

[0052] 11. When the fruiting body grows spores, it means it is mature and can be harvested. After harvesting, it can be eaten fresh or dried and made into powder or various products.

[0053] Example 2 1. Choose several fresh duck eggs. The laying date of each egg should not exceed 1 week. Make sure the eggs are not damaged.

[0054] 2. Disinfect the duck egg shells with 75% alcohol and place them on a clean workbench for preparation.

[0055] 3. Use a sterilized duck egg puncher in a clean bench to make a pinhole in the big end of the duck egg. Use a sterile needle-like tool to enter the egg to break the yolk membrane, and shake to mix the yolk and egg white evenly.

[0056] 4. Use a sterilized injection needle to extract 15-20ml of duck egg liquid from the egg hole, and the extracted egg liquid accounts for 20-25%.

[0057] 5. Inject 6 ml of 40% sterilized sucrose aqueous solution into the duck eggs from which the egg liquid has been extracted, and then shake the duck eggs to mix the duck egg liquid and glucose evenly.

[0058] 6. Add 3 ml of Cordyceps militaris liquid culture, then continue to shake evenly and stick the pinholes.

[0059] 7. Place the duck eggs with the Cordyceps militaris liquid spawn in the egg tray with the pinhole facing upwards, and then culture them in a room with a temperature of 20-25 degrees and a humidity of 40-60% for 15-30 days.

[0060] 8. After 15-30 days, shake each duck egg. If there is no liquid flowing inside, disinfect the duck egg. If there is liquid flowing inside after 30 days, it may be contaminated and the egg should be discarded.

[0061] 9. Disinfect the outside of the egg with 75% alcohol, then use an egg opener to remove a part of the eggshell with the pinhole head, put it into the egg tray in a sterilized transparent plastic box with the opening facing up, cover the transparent plastic box with the lid, and place it in a room with a temperature of 20-25 degrees and a humidity of 65-85%, and cultivate it under light intensity of 500-1000LX.

[0062] 10. Generally, the Cordyceps pupae can emerge from the base after 10-15 days of cultivation, and the fruiting body will mature after another 25-30 days of cultivation.

[0063] 11. When the fruiting body grows spores, it means it is mature and can be harvested. After harvesting, it can be eaten fresh or dried and made into powder or various products.

Claims

1. A method for cultivating Cordyceps sinensis, characterized in that: include: S1, select fresh eggs; S2, forming a small hole on the eggshell, breaking the egg yolk through the small hole, and extracting a certain amount of egg liquid; S3, injecting sterilized sugar solution into the egg from which the egg liquid has been extracted, and then shaking the egg to mix the egg liquid with the sugar solution; S4, inoculating the mixed eggs with liquid Cordyceps sinensis, shaking them evenly, and sealing the small holes; S5, placing the eggs inoculated with Cordyceps liquid spawn in an environment with a temperature of 22-25 degrees and a humidity of 40%-60% for 10-30 days; S6, remove part of the eggshell from the cultured eggs, put them into a sterilized transparent container, fix them and seal them, place them in an environment with a temperature of 20-25 degrees and a humidity of 65%-85%, and culture them under light until they grow grass and mature, thereby obtaining egg Cordyceps sinensis.

2. The method for cultivating egg cordyceps according to claim 1, characterized in that: In step S1, the fresh eggs are chicken eggs, duck eggs, goose eggs, quail eggs or pigeon eggs, preferably chicken eggs.

3. The method for cultivating the egg cordyceps according to claim 1, characterized in that: After step S1 and before step S2, the method further includes: The eggshells of the eggs were disinfected with 75% alcohol and placed in a clean bench.

4. The method for cultivating the egg cordyceps according to claim 1, characterized in that: In step S2, the small hole is formed on the large end of the egg; the volume of the extracted egg liquid is 15%-30% of the total volume of the egg liquid.

5. The method for cultivating the egg cordyceps according to claim 1, characterized in that: In step S3, the sterilized sugar aqueous solution is glucose, sucrose or fructose solution, with a concentration of 30%-50% and a volume of 30%-50% of the volume of the extracted egg liquid.

6. The method for cultivating the egg cordyceps according to claim 1, characterized in that: In step S4, the liquid Cordyceps spawn is Cordyceps militaris or Cordyceps militaris, and the volume is 3%-5% of the total volume of the egg liquid.

7. The method for cultivating the egg cordyceps according to claim 1, characterized in that: In step S6, the egg is shaken. If no liquid flows in the egg, it means that the egg is a well-cultured egg; if liquid flows in the egg, it means that the egg is not well-cultured.

8. The method for cultivating egg cordyceps according to claim 4, characterized in that: In step S6, the removed eggshell is the eggshell near the small hole. When fixing the egg, an egg tray is placed in a transparent container, and the egg is placed in the egg tray with the end with the eggshell removed facing upward.

9. The method for cultivating Cordyceps sinensis according to claim 1, characterized in that: In step S6, the light intensity of the culture is 200-1000LX, the grass grows after 7-15 days of culture, and matures after 20-40 days of culture. When the fruiting body grows spores, it indicates that it is mature.

10. A kind of egg cordyceps, characterized in that: The method is adopted to obtain the culturing method as claimed in any one of claims 1 to 9.