9 results about "Inbreeding" patented technology

The application discloses a goldfish molecular marker assisted breeding method, relates to the ornamental fish genetic breeding technical field, and aims at overcoming the defects of inbred degeneration and uncontrollable germplasm in new strain breeding. The method comprises the following steps: selecting a wishful eye goldfish with wishful eye traits and carrying first and second molecular markers in homozygosis as a first parent, and selecting a skin ball pearl goldfish with pearl eye traits as a second parent; performing the first parent and the second parent with normal and reverse crossing to construct two hybrid families; performing brother-sister intercross of F1 generation of the two families; screening double marker homozygous individuals; screening a candidate wishful pearl strain with wishful eye and pearl eye traits; and performing interfamily hybridization of the candidate strains of the two families to obtain a target goldfish new strain. The application can overcome the germplasm degeneration caused by inbreeding, and obtain a new goldfish strain with visible pupil and round and beautiful body shape when looking down, i.e., the wishful pearl, and realize germplasm tracking and intellectual property protection through molecular markers.

The invention discloses a shrimp individual inbreeding coefficient optimal evaluation parameter construction method based on a long homozygous fragment, and belongs to the technical field of molecular breeding of aquatic animals, and the method comprises the following steps: (1) carrying out SNP screening and typing on re-sequencing data of a shrimp sample by using a corresponding shrimp reference genome to obtain a corresponding number of whole genome SNP markers; (2) setting non-genome specific parameters and establishing a simulated sliding window database; (3) setting genome specific parameters; and (4) summarizing optimal evaluation parameters. The method does not depend on the quality of a reference genome, optimal parameters can still be found under the current fragmentation and low splicing quality conditions, ROH fragments are accurately detected, and the real inbreeding coefficient is accurately evaluated; meanwhile, the method can be popularized to other reference genomes with different splicing qualities, and corresponding optimal parameters are obtained.

The invention provides a guiding method and system for detecting animal inbreeding level based on a whole genome sequencing method, and belongs to the field of genomics and molecular biology. The problem that errors are large when ROH detection is carried out on low-quality data is solved. The method comprises the following steps: acquiring real reference genome data and whole genome sequencing data, and performing preprocessing, namely screening autosomes without base deletion sequences to construct a new reference genome, calculating real inbreeding level parameters and generating a haplotype genome file; the simulation whole genome sequencing data set comprises a sequencing depth gradient distribution data set, a read length gradient distribution data set and a reference genome continuity gradient distribution data set; respectively carrying out homozygous fragment detection by using the simulated whole genome sequencing data set so as to evaluate the influence of data quality parameters on the detection accuracy; and optimizing homozygous fragment detection software parameters based on an evaluation result. The method is used in the field of inbreeding evaluation of endangered species and small populations.

The application discloses a method for constructing a hyperlipidemia-resistant rat model, and belongs to the technical field of experimental animal model construction. S1, basic population screening: abnormal lipid metabolism rats are selected, and the rats are fed with high-fat feed at the age of 4 weeks, and the feeding is continuously performed for 4 weeks; S2, blood lipid detection and preliminary screening: the animals are fasted for 12h, blood is taken from the submandibular vein, serum is separated by centrifugation at 3000r / min for 10min, and the total cholesterol, triglyceride, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol contents are detected by using an automatic biochemical analyzer; S3, paired reproduction and subculture: 30 male rats and 30 female rats are selected, and the rats are paired for reproduction at a ratio of 1:1 to obtain F1 generation; S4, inbreeding and stabilization: the F2-F4 generation rats are repeatedly fed, detected, screened and paired for reproduction according to steps S1-S3, and the F4 generation hyperlipidemia-resistant rat model is obtained through inbreeding, and the technical effect of obtaining the hyperlipidemia-resistant rat model which is stable in phenotype and can be inherited and subcultured is achieved through the combination of hybridization screening and inbreeding.

The application provides a genetic economic trait parameter estimation method of Epinephelus akaara, and relates to the technical field of Epinephelus akaara.The application firstly divides Epinephelus akaara into multiple genetic clusters based on SNP information through principal component analysis and spectral clustering algorithm;then obtains the half lethal temperature and disease resistance comprehensive score of each cluster through high-temperature tolerance experiment and disease resistance experiment;carries out breeding test under different environmental conditions, calculates growth resistance in combination with environmental fitness, and obtains growth evaluation index by comprehensively considering the growth rate under the standard environment;finally, the comprehensive genetic score is calculated by integrating the growth evaluation index, the half lethal temperature and the disease resistance score, and the top 20% excellent clusters are screened out.In the excellent clusters, the individuals are initially selected according to the growth rate, heat tolerance and disease resistance phenotype data, then the families are divided by SNP information to avoid inbreeding, and finally the BLUP method is used to calculate the comprehensive breeding value, and the top 20% individuals are selected as parents.

This invention discloses an animal mating method, system, device, and storage medium based on pedigree relationships and predicted inbreeding coefficients for offspring. The mating method includes: S1, acquiring animal pedigree data, male animal individual data, and female animal individual data, wherein the pedigree data includes at least an individual identifier, a paternal identifier, and a maternal identifier; S2, performing legality checks and structured processing on the pedigree data, wherein the legality checks include at least one of the following: duplicate individual identifier checks, checks where the paternal or maternal parent is equal to the individual itself, checks where the parents are missing from the pedigree, topological anomaly checks, and circular dependency checks. Compared with existing technologies, this invention clearly distinguishes between existing individual inbreeding coefficients and predicted inbreeding coefficients for future offspring of candidate males and females, improving the accuracy of mating criteria.

The invention discloses a rapid detection method for family composition of a bee colony, and belongs to the technical field of genetic breeding of bees. The method comprises the following steps: randomly collecting a plurality of worker bee individuals of a to-be-detected bee colony, respectively intercepting the right posterior foot tissue of each worker bee, mixing and grinding, and extracting total DNA to obtain a unified colony DNA sample; carrying out whole genome sequencing on the sample, comparing a sequencing read segment to a csd gene region, and calculating the frequency of each allele; alleles of queen bees and drone sources are distinguished according to frequency distribution, and the composition proportion of each paternal family is calculated based on the frequency of the alleles of the drone sources. According to the method, the right posterior foot tissue is intercepted in a standardized manner, so that the relative constancy of the individual DNA amount in the mixed sample is ensured, and the quantization deviation is eliminated; and in combination with a high-throughput sequencing strategy, the complex family structure of the bee colony and the potential inbreeding risk can be quickly and accurately analyzed at low cost only by one-time sequencing.

The invention relates to a multi-gene pyramiding breeding method suitable for animal populations, the method adopts a minimum rotation maximum complementation (mRMC) gene pyramiding method, compared with the prior art, all individuals in the population can stably carry n target genes (ideal populations) at the same time by needing fewer generations, the number of generations is reduced, the number of generations is reduced, the number of generations is reduced, and the number of generations is reduced. And it is ensured that the group still contains n families. According to the method, the gene polymerization efficiency is effectively improved, and the method shows superiority in the aspects of controlling the population inbreeding level, maintaining the population genetic diversity, maintaining the background character genetic progress and the like. The method. The method can be combined with breeding technologies such as genome selection, marker-assisted selection, comprehensive selection indexes and the like, provides an efficient, controllable and programmable general technical framework for polygene pyramiding breeding of livestock and poultry such as pigs, cattle, sheep, chickens and the like, and has important application value for accelerating the molecular design breeding process and breeding new varieties with high resistance and high yield.

The invention provides a pelicans paternity test method based on a microsatellite marker, a primer group and application, and relates to the technical field of biological paternity test. The paternity test method comprises the following steps: extracting genome DNA (deoxyribonucleic acid) of a to-be-identified pelicans offspring, a known parent and a candidate parent; carrying out PCR (Polymerase Chain Reaction) amplification by using the specific primer pair by taking the genome DNA as a template to obtain an amplification product; detecting the fragment size of the amplification product to obtain a genotype; and comparing the genotypes to judge the parent-child relationship. According to the method, genetic typing is carried out on samples such as the pelicans feathers by using the specific microsatellite primers, the misjudgment risk of a behavioral observation method is overcome on the basis of a codominant genetic law, parent sources can be accurately traced, and non-biological parents can be excluded; according to the method, a definite molecular identification basis is provided, establishment of accurate family archives and evaluation of genetic diversity are facilitated, and reliable technical support is provided for scientific breeding management and inbreeding avoidance of pelicans.