19 results about "Inbreeding" patented technology

The application discloses a goldfish molecular marker assisted breeding method, relates to the ornamental fish genetic breeding technical field, and aims at overcoming the defects of inbred degeneration and uncontrollable germplasm in new strain breeding. The method comprises the following steps: selecting a wishful eye goldfish with wishful eye traits and carrying first and second molecular markers in homozygosis as a first parent, and selecting a skin ball pearl goldfish with pearl eye traits as a second parent; performing the first parent and the second parent with normal and reverse crossing to construct two hybrid families; performing brother-sister intercross of F1 generation of the two families; screening double marker homozygous individuals; screening a candidate wishful pearl strain with wishful eye and pearl eye traits; and performing interfamily hybridization of the candidate strains of the two families to obtain a target goldfish new strain. The application can overcome the germplasm degeneration caused by inbreeding, and obtain a new goldfish strain with visible pupil and round and beautiful body shape when looking down, i.e., the wishful pearl, and realize germplasm tracking and intellectual property protection through molecular markers.

The application discloses a goldfish molecular marker assisted breeding method, relates to the ornamental fish genetic breeding technical field, and aims at overcoming the defects of inbred degeneration and uncontrollable germplasm in new strain breeding. The method comprises the following steps: selecting a wishful eye goldfish with wishful eye traits and carrying first and second molecular markers in homozygosis as a first parent, and selecting a skin ball pearl goldfish with pearl eye traits as a second parent; performing the first parent and the second parent with normal and reverse crossing to construct two hybrid families; performing brother-sister intercross of F1 generation of the two families; screening double marker homozygous individuals; screening a candidate wishful pearl strain with wishful eye and pearl eye traits; and performing interfamily hybridization of the candidate strains of the two families to obtain a target goldfish new strain. The application can overcome the germplasm degeneration caused by inbreeding, and obtain a new goldfish strain with visible pupil and round and beautiful body shape when looking down, i.e., the wishful pearl, and realize germplasm tracking and intellectual property protection through molecular markers.

The invention discloses an inbreeding analysis method and system for high-throughput sequencing and electronic equipment, and relates to the technical field of animal molecule detection.The inbreeding analysis method comprises the steps that an adult inbreeding boar sample set and a southern Yunnan boar sample set are obtained, RNA extraction is conducted, and an adult inbreeding boar sample RNA set and a southern Yunnan boar sample RNA set are obtained; performing sample quality comprehensive authentication on the adult inbred boar sample RNA set and the southern Yunnan boar sample RNA set according to a preset multi-dimensional sample RNA quality evaluation weight set to obtain an authentication result; and when the authentication result is that the authentication is passed, performing high-throughput sequencing based on the adult inbred boar sample RNA set and the southern Yunnan boar sample RNA set, and performing inbreeding analysis according to the sequencing result. The technical problem of poor reliability of sequencing data in pig breed inbreeding analysis in the prior art is solved, and the technical effect of improving high-throughput sequencing accuracy and inbreeding analysis result credibility is achieved.

The invention discloses a shrimp individual inbreeding coefficient optimal evaluation parameter construction method based on a long homozygous fragment, and belongs to the technical field of molecular breeding of aquatic animals, and the method comprises the following steps: (1) carrying out SNP screening and typing on re-sequencing data of a shrimp sample by using a corresponding shrimp reference genome to obtain a corresponding number of whole genome SNP markers; (2) setting non-genome specific parameters and establishing a simulated sliding window database; (3) setting genome specific parameters; and (4) summarizing optimal evaluation parameters. The method does not depend on the quality of a reference genome, optimal parameters can still be found under the current fragmentation and low splicing quality conditions, ROH fragments are accurately detected, and the real inbreeding coefficient is accurately evaluated; meanwhile, the method can be popularized to other reference genomes with different splicing qualities, and corresponding optimal parameters are obtained.

The invention provides a guiding method and system for detecting animal inbreeding level based on a whole genome sequencing method, and belongs to the field of genomics and molecular biology. The problem that errors are large when ROH detection is carried out on low-quality data is solved. The method comprises the following steps: acquiring real reference genome data and whole genome sequencing data, and performing preprocessing, namely screening autosomes without base deletion sequences to construct a new reference genome, calculating real inbreeding level parameters and generating a haplotype genome file; the simulation whole genome sequencing data set comprises a sequencing depth gradient distribution data set, a read length gradient distribution data set and a reference genome continuity gradient distribution data set; respectively carrying out homozygous fragment detection by using the simulated whole genome sequencing data set so as to evaluate the influence of data quality parameters on the detection accuracy; and optimizing homozygous fragment detection software parameters based on an evaluation result. The method is used in the field of inbreeding evaluation of endangered species and small populations.

The application discloses a method for constructing a hyperlipidemia-resistant rat model, and belongs to the technical field of experimental animal model construction. S1, basic population screening: abnormal lipid metabolism rats are selected, and the rats are fed with high-fat feed at the age of 4 weeks, and the feeding is continuously performed for 4 weeks; S2, blood lipid detection and preliminary screening: the animals are fasted for 12h, blood is taken from the submandibular vein, serum is separated by centrifugation at 3000r / min for 10min, and the total cholesterol, triglyceride, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol contents are detected by using an automatic biochemical analyzer; S3, paired reproduction and subculture: 30 male rats and 30 female rats are selected, and the rats are paired for reproduction at a ratio of 1:1 to obtain F1 generation; S4, inbreeding and stabilization: the F2-F4 generation rats are repeatedly fed, detected, screened and paired for reproduction according to steps S1-S3, and the F4 generation hyperlipidemia-resistant rat model is obtained through inbreeding, and the technical effect of obtaining the hyperlipidemia-resistant rat model which is stable in phenotype and can be inherited and subcultured is achieved through the combination of hybridization screening and inbreeding.

The application discloses a breeding method of a new variety of red chirochirus, and relates to the technical field of breeding of new varieties of shrimps.The breeding steps are as follows: (1) parent cross: male chirochirus of yellow abdomen and female common chirochirus are used as parent shrimps to cross, and F1 generation is produced; (2) first in-family inbreeding: the chirochirus of yellow abdomen in the F1 generation is selected to mate with the chirochirus of yellow abdomen, and F2 generation is produced; (3) second in-family inbreeding: the red chirochirus in the F2 generation is selected to mate with the red chirochirus, and F3 generation is obtained, and the F3 generation is the new variety of the red chirochirus.The new variety of the red chirochirus with red body color can be bred, the new variety of the chirochirus is rich in types, the new variety has good growth performance, and has remarkable commercial traits, and can be popularized for breeding.

The invention provides a method for constructing a genome selective breeding technical system of downy sheep, and relates to the technical field of genome selective breeding of downy sheep, the method comprises the following steps: constructing a reference group, selecting samples from downy sheep with multiple regions and multiple genetic backgrounds, collecting phenotypic data, and carrying out genetic typing; then establishing a genome selection model, processing genotype and phenotype data, and training by using GBLUP and Bayesian models; then, candidate groups are screened, a genome estimation breeding value is calculated, and individuals ranked in the first 20%-30% are selected; a breeding scheme is formulated, breeding is carried out without too large inbreeding coefficient, and progenies are determined and analyzed; and finally, collecting new data every year, and retraining the model every 2-3 years. Regional ecological differences of a reference group, a model algorithm, a convergence threshold value and candidate group phenotype evaluation index weights are limited, and the breeding effect of the villus sheep can be optimized.

The invention belongs to but is not limited to the technical field of pest biological control, and discloses a method for relieving reproductive decline of harmonia axyridis, which is suitable for indoor long-term breeding populations incapable of avoiding inbreeding, in particular to rare homozygous mutants needing long-term conservation. The method specifically comprises the following steps: feeding offspring new eclosion adults which show reproductive decline after being fed with aphids as food with artificial feed prepared by mixing pork liver homogenate, rape pollen, cane sugar, yeast powder, honey and the like for 3-4 days, and then feeding the adults with the aphids; in the feeding process after the ladybirds start to lay eggs, 7-day aphids and 1-day artificial feed are adopted for feeding circularly, and high reproductive power is kept. In addition, when severely reproductive decline occurs to adults fed with the aphids in daily life, the adults are fed with artificial feed for 3 days in time, then aphid feeding is recovered, and the egg laying amount and the egg hatching rate can be remarkably increased within a short time. According to the method, the reproductive decline of the harmonia axyridis bred indoors for a long time is effectively relieved through short-term intermittent artificial feed feeding.

The application provides a genetic economic trait parameter estimation method of Epinephelus akaara, and relates to the technical field of Epinephelus akaara.The application firstly divides Epinephelus akaara into multiple genetic clusters based on SNP information through principal component analysis and spectral clustering algorithm;then obtains the half lethal temperature and disease resistance comprehensive score of each cluster through high-temperature tolerance experiment and disease resistance experiment;carries out breeding test under different environmental conditions, calculates growth resistance in combination with environmental fitness, and obtains growth evaluation index by comprehensively considering the growth rate under the standard environment;finally, the comprehensive genetic score is calculated by integrating the growth evaluation index, the half lethal temperature and the disease resistance score, and the top 20% excellent clusters are screened out.In the excellent clusters, the individuals are initially selected according to the growth rate, heat tolerance and disease resistance phenotype data, then the families are divided by SNP information to avoid inbreeding, and finally the BLUP method is used to calculate the comprehensive breeding value, and the top 20% individuals are selected as parents.

The invention relates to the field of fish breeding, and discloses a breeding method of a high-temperature-resistant new species of Halitium larimichthys crocea body, which comprises the following steps: performing sample collection on cultured Halitium larimichthys crocea, and screening high-temperature-resistant samples based on parameters such as survival rate and heat stress physiological indexes; the genetic potential of the high-temperature-resistant sample is further evaluated in a parent genetic evaluation mode, and the purpose of screening a core breeding sample is achieved. Finally, seedling raising is carried out by designing a low-inbreeding pairing scheme, heat-resistant advantage related genes are polymerized, and the purpose of fixing high-temperature-resistant characters is achieved through continuous breeding. The method can break through the bottleneck that the sal-family pseudosciaena crocea dies at a high temperature, realizes cultivation of a new high-temperature-resistant variety of the sal-family pseudosciaena crocea, and provides sustainable provenance support for expanding a pseudosciaena crocea culture space and a marine fish culture industry under a warming background.

This invention discloses an animal mating method, system, device, and storage medium based on pedigree relationships and predicted inbreeding coefficients for offspring. The mating method includes: S1, acquiring animal pedigree data, male animal individual data, and female animal individual data, wherein the pedigree data includes at least an individual identifier, a paternal identifier, and a maternal identifier; S2, performing legality checks and structured processing on the pedigree data, wherein the legality checks include at least one of the following: duplicate individual identifier checks, checks where the paternal or maternal parent is equal to the individual itself, checks where the parents are missing from the pedigree, topological anomaly checks, and circular dependency checks. Compared with existing technologies, this invention clearly distinguishes between existing individual inbreeding coefficients and predicted inbreeding coefficients for future offspring of candidate males and females, improving the accuracy of mating criteria.

The invention relates to the technical field of animal breeding, and particularly discloses a beef cattle genome matching method integrating genetic advantages and defects and application, the method comprises the following steps: S1, respectively extracting beef cattle individual DNA samples from to-be-matched breeding bulls and to-be-matched cows for genetic typing, and obtaining individual SNP genotype data; s2, calculating an additive genetic relationship matrix according to a VanRaden algorithm to obtain genome estimation breeding values of beef cattle individuals on important economic characters, and calculating comprehensive selection indexes of the individuals; s3, according to the comprehensive selection index value of the individual, the SNP genotype data, the inbreeding coefficient and the economic value corresponding to each part, constructing a beef cattle economic scoring model; s4, based on the beef cattle economic scoring model, calculating economic scores of all possible pairing combinations between the to-be-mated breeding bulls and the to-be-mated cows, and performing maximum solution to generate an optimal mating scheme; through genome breeding value evaluation and economic scoring model construction, beef cattle genome matching with genetic advantages and defects is realized.

The invention discloses a rapid detection method for family composition of a bee colony, and belongs to the technical field of genetic breeding of bees. The method comprises the following steps: randomly collecting a plurality of worker bee individuals of a to-be-detected bee colony, respectively intercepting the right posterior foot tissue of each worker bee, mixing and grinding, and extracting total DNA to obtain a unified colony DNA sample; carrying out whole genome sequencing on the sample, comparing a sequencing read segment to a csd gene region, and calculating the frequency of each allele; alleles of queen bees and drone sources are distinguished according to frequency distribution, and the composition proportion of each paternal family is calculated based on the frequency of the alleles of the drone sources. According to the method, the right posterior foot tissue is intercepted in a standardized manner, so that the relative constancy of the individual DNA amount in the mixed sample is ensured, and the quantization deviation is eliminated; and in combination with a high-throughput sequencing strategy, the complex family structure of the bee colony and the potential inbreeding risk can be quickly and accurately analyzed at low cost only by one-time sequencing.

The invention relates to the field of fish breeding, and discloses a body quality character breeding method and system for Pseudosciaena crocea of the sal-ammoniac family, and the method comprises the following steps: determining SNP key sites related to the body quality character of the Pseudosciaena crocea of the sal-ammoniac family in a manner of performing sample collection and phenotype data combined genome analysis on the Pseudosciaena crocea of the sal-ammoniac family; a genotype-body quality phenotype correlation model is constructed according to the sites, dominant genotype parent combinations are screened, high-quality fries are bred, breeding contrast experiments are carried out, and the purpose of breeding the Larimichthys crocea of the sal family is achieved. According to the method, a core breeding group can be established through artificial breeding, the loss of genetic diversity caused by inbreeding of wild sal ammoniac is avoided, specific genes are preserved, and the breeding of the genetic improved variety of the sal ammoniac pseudosciaena crocea with remarkable growth advantages is realized.

The invention relates to a multi-gene pyramiding breeding method suitable for animal populations, the method adopts a minimum rotation maximum complementation (mRMC) gene pyramiding method, compared with the prior art, all individuals in the population can stably carry n target genes (ideal populations) at the same time by needing fewer generations, the number of generations is reduced, the number of generations is reduced, the number of generations is reduced, and the number of generations is reduced. And it is ensured that the group still contains n families. According to the method, the gene polymerization efficiency is effectively improved, and the method shows superiority in the aspects of controlling the population inbreeding level, maintaining the population genetic diversity, maintaining the background character genetic progress and the like. The method. The method can be combined with breeding technologies such as genome selection, marker-assisted selection, comprehensive selection indexes and the like, provides an efficient, controllable and programmable general technical framework for polygene pyramiding breeding of livestock and poultry such as pigs, cattle, sheep, chickens and the like, and has important application value for accelerating the molecular design breeding process and breeding new varieties with high resistance and high yield.

The invention discloses a matching method for integrating genome selection and application, and belongs to the field of genetic resource protection and genetic improvement of local livestock and poultry. In order to solve the problems that existing genome selection focuses on genetic progress and neglects population diversity, and genome selection is mostly used for hybridization production and adaptive purebred conservation is insufficient, the method comprises the following steps: firstly, constructing a simulated breeding population and about 2000 reference populations represented by Ningxiang pigs, and measuring pedigree, phenotype and SNP chip genotype data; calculating candidate individual GEBV based on an ssGBLUP model, screening and reserving seeds, and establishing a target function comprehensively considering selection progress, heterozygosity, an inbreeding coefficient and the like to implement matching; and evaluating an effect through a multi-dimensional index and comparing a comparison strategy. The method realizes the balance of breed conservation and genetic improvement of the local livestock and poultry, can effectively control inbreeding and preserve genetic diversity, provides an effective technical means for breeding of the local livestock and poultry, and has wide reference significance.

The invention provides a pelicans paternity test method based on a microsatellite marker, a primer group and application, and relates to the technical field of biological paternity test. The paternity test method comprises the following steps: extracting genome DNA (deoxyribonucleic acid) of a to-be-identified pelicans offspring, a known parent and a candidate parent; carrying out PCR (Polymerase Chain Reaction) amplification by using the specific primer pair by taking the genome DNA as a template to obtain an amplification product; detecting the fragment size of the amplification product to obtain a genotype; and comparing the genotypes to judge the parent-child relationship. According to the method, genetic typing is carried out on samples such as the pelicans feathers by using the specific microsatellite primers, the misjudgment risk of a behavioral observation method is overcome on the basis of a codominant genetic law, parent sources can be accurately traced, and non-biological parents can be excluded; according to the method, a definite molecular identification basis is provided, establishment of accurate family archives and evaluation of genetic diversity are facilitated, and reliable technical support is provided for scientific breeding management and inbreeding avoidance of pelicans.

The invention provides a sea-area lateolabrax japonicus genetic economic character parameter estimation method, and relates to the technical field of sea-area lateolabrax japonicus. The sea-area lateolabrax japonicus genetic economic character parameter estimation method comprises the steps that firstly, based on SNP information, lateolabrax japonicus is divided into a plurality of genetic clusters through principal component analysis and a spectral clustering algorithm; respectively obtaining a half lethal temperature and a disease resistance comprehensive score of each cluster through a high temperature resistance experiment and a disease resistance experiment; breeding tests are carried out under different environmental conditions, growth resistance is calculated in combination with environmental fitness, and growth evaluation indexes are obtained by integrating growth rates under a standard environment; and finally, integrating the growth evaluation index, the half lethal temperature and the disease resistance score to calculate a comprehensive genetic score, and screening out first 20% of excellent clusters. In excellent clustering, individuals are primarily selected according to phenotype data of growth rate, heat resistance and disease resistance, families are divided through SNP information to avoid inbreeding, a comprehensive breeding value is calculated by adopting a BLUP method, and the first 20% of individuals are selected as parents.