120 results about "Inbreeding" patented technology

The invention discloses a method for breeding a new golden-shell crassostrea gigas strain. The method is characterized by including the operating steps of (1) breeding the first generation: selecting wild parent crassostrea gigas with golden left shells, and creating a plurality of families without genetic relationships; (2) breeding the second generation: selecting the families with the obvious golden-shell characters from the created first-generation families, and screening out the crassostrea gigas with the golden left shells and the golden right shells to carry out inner-family inbreeding; (3) breeding the third generation: carrying out inter-family hybridization on the families, with the left shells and the right shells being in the golden characters, obtained from the created second-generation families; (4) breeding the fourth generation: carrying out stability verification on the families with the left shells and the right shells being in the golden characters. According to the method, the breeding process of the new crassostrea gigas shell-color strain is accelerated, and new products of bred shellfishes are increased; the product additional value is increased through the color characters, the breeding yield is improved through the growth characters, incomes of breeding enterprises and farmers are increased, and the market prospects are broad.

A breeding method for the new variety of the carp relates to a breeding method of a new variety of the fish, which resolves the problems of the prior group breeding method for the culture and the cultivation of the carp that is easy to have the inbreeding, which leads to the decrease of the economic character and the production performance; the intraspecific hybridization method has great randomness, the probability for breeding an excellent variety is low and is easy to have the inbreeding. The breeding procedures are: 1. performing the phenotypic character selection; 2. detecting the genetic information of the parent strain; 3. performing the reproduction grouping according to the genetic information; 4. cultivating the filial generation to be matured; 5. adopting the offspring carp as the new generation of the breeding parent strain and repeating the operation procedures from 1 to 4 until the carp meets the character design requirements of the new variety. The present invention always uses the molecular marker to detect the genetic composition of the parent strain and the filial generation in the selection process of the new carp variety, thereby avoiding the inbreeding; moreover the character of the new variety of the carp is developed in the designed direction, the emergence probability of the excellent new variety is high; therefore, the economic character and the production performance of the carps are obviously improved.

The invention discloses a breeding method for edible high-quality ion-zinc-rich rice. The breeding method comprises the following steps: selecting red rice variety as a parent A, selecting zinc-rich rice variety as a parent B, and selecting glutinous rice variety as a parent C, using the parent A as the female parent, using the parent B as the male parent, hybridizing, and obtaining the first generation F1 of A / B; using the F1 as the female parent, using the parent C as the male parent, hybridizing, and obtaining the first generation F1 of A / B / / C; selfing the F1, and obtaining the second generation F2; selfing the F2, performing the planting and harvesting according to the single plant, successively using the red rice, the glutinous property and the zinc-rich as the sieving indexes, and obtaining the third generation F3; performing the inbreeding of more generation to the F3, and obtaining the homozygous later generation with the red rice, glutinous property and zinc-rich characters; performing the inbreeding of more generation to the homozygous later generation, and obtaining the later generation with good agronomic characters; and performing the inbreeding of more generation to the later generation, breeding a strain with stable character, and obtaining the edible high-quality ion-zinc-rich rice. The cultivated rice variety belongs to the red rice variety, is edible in high quality and contains iron and zinc richly.

The invention discloses a cement pit breeding method of langoustine, comprising the steps of seed selection, mating, hatching, juvenile langoustine breeding and langoustine seed catching. According to the invention, the seed selection standard of the parent langoustine is strict, and the old, weak, sick and disable parent langoustine are sifted out, thereby laying foundation for breeding health juvenile langoustine with high quality; since the parent langoustine used for breeding is different in resource, inbreeding is avoided, and the quality of the juvenile langoustine is ensured; oogenesis parent langoustine refers to oogenesis parent langoustine laying egg in the same period, the yield is high and the langoustine seeds are kept consistent in size; the disease resistance of the langoustine seed is strong, the loss of langoustine workers is reduced, and the cultivation benefit is increased; furthermore, according to the invention, parent langoustine transfer is simple and convenient, the langoustine seed catching is simple and feasible, and the labor intensity is greatly reduced.

The invention discloses a breeding method for a sepia esculenta of quick-growing strain, which belongs to the technical field of breeding aquatic fingerlings, and comprises the following steps: basic populations building, mating plan making, brood stock domesticating and strengthening cultivation, brood stock mating, eggs laying, fingerlings cultivating, fingerlings developing, fingerlings selecting, brood stock selecting and remaining, and next-generation breeding plan making. According to the method, by means of hybridizing between populations, the method takes full advantage of the hybridization to allow excellent characters of the sepia esculenta to be expressed, and meanwhile utilizes autocopuation in a population without controlling inbreeding coefficient to allow excellent genes with same hereditary basis to be subjected to homozygosis; through measurements and analyses for growing indexes in the sepia esculenta breeding process, and a male and a female with equivalent brood stock growth value can mate to conduct lay eggs for the next generation; and the sepia esculenta quick-growing character is bred in a plurality of generations to enable excellent characters of different populations to gather, so as to breed the sepia esculenta of quick-growing strain.

The invention discloses a chicken preserved population individual apolegamy optimizing method. Blood DNA of chicken preserved population breed reserving cock and hen individuals is extracted, sequencing is carried out through a reduced genomic sequencing technology, and the sequence is compared with a sequence of the GenBank database reference chicken breed to find SNP loci existing in different individuals. The individual own inbreeding coefficient and the interindividual phylogenetic relationship coefficient are calculated through gene frequency and genotype frequency of the SNP loci, individual apolegamy is optimized according to the statistics value, mating combination of individuals with an overlarge inbreeding coefficient and individuals with an overlarge within-family phylogenetic relationship coefficient is avoided, and the purposes of reducing the preserved population inbreeding level and maintaining the genetic diversity total quantity steady state are achieved. By means of the method, the problem that fine individual apolegamy cannot be carried out depending on genealogy information in the chicken breed resource protection process can be effectively solved, the preserved population inbreeding level can be effectively reduced, the genetic diversity steady state is maintained, and the breed preservation effect and efficiency are improved.

The invention discloses a method for breeding inbred line by using sweet potato special materials. The method comprises a special material selection and isolation method, manpower-assisted inbreeding and inbreeding offspring testcross selection. In the invention, a sweet potato special resource which blooms and fructifies naturally and has wide compatibility is taken as a parent material, a plurality of systems with different genetic backgrounds are established at the same time, the inbreeding is performed strictly and the hybridization is avoided by taking isolation measures; the character of blooming and fructifying naturally and the target economical character are successively selected, so as to breed a inbred line material which has excellent target characters and can bloom and fructify naturally stably in the north area in China, the testcross of the inbred line materials is used for identifying the heterosis and the excellent inbred line is used for crossbreeding.

The invention provides a family breeding method for pearl white strain red tilapia. The method includes the steps that (1) parent fish selection is conducted, the red tilapia with the pearl white body surface and the whole body being free of red spots or black spots is picked out from a red tilapia group with serious body color differentiation, and the red tilapia with obvious growth advantages is used as standby parents; (2) family establishment is conducted, the selected male and female parents are placed into a net cage for carrying out adaptation reinforcement cultivation, and manual orientation mating, hatching and seedling cultivating are independently performed according to the proportion of one to one; (3) pearl white strain breeding of the red tilapia is conduced, the growing juvenile fish stage and the adult fish stage of the red tilapia are determined, the pearl white red tilapia family with the high proportion and good growth traits are screened out to be used as the breeding base family of the next generation, 2-4 generation breeding is conducted according to the method, and accordingly the pearl white strain red tilapia can be bred. By the adoption of the method, inbreeding is avoided, the variety degeneration of the red tilapia is effectively prevented, and the population genetic diversity is kept; the breeding process is accelerated, and the breeding efficiency is improved.

The invention discloses a purification and rejuvenation method of sheep and goat breeds. The purification and rejuvenation method adopts a core group open type breeding method for establishing a core group and an underlying group among peasant households and realizes the transform from a small scale of the core group of conventional field construction, breed protection and breeding to a large scale of the core group of open type breeding established among the peasant households, fully reflects the breeding advantages of large core group, high frequency of elite genotypes, wide influence surface and wide radiation scope, and improves the production level of the breed protection and breeding group faster; the purification and rejuvenation method realizes the transform from the conventional field construction, breed protection and breeding with high maintenance cost and difficulty in long-term preservation of the core group to the dispersed establishment of the core group among the peasant households, with easiness in maintenance, long-term preservation and consolidation, fully reflects the advantages of large group and small breeding risk, and enables the development of the breed protection and breeding group to have a solid foundation; and the inbreeding coefficient of the open type breeding is slow in increase and difficult to cause the inbreeding depression phenomenon.

The invention belongs to the crop breeding field, and concreatly relates to a method for rapidly cultivating transgene maize inbred line. The method comprises the following steps: 1)operating transgene base material: performing hybridization of a transgene positive material and an excellent maize inbred line; 2)performing hybrid induction: performing hybridization of an induction line and the transgene base material; 3)performing haploid selection: selecting a haploid plant through grain and field plant; 4) doubling haploid: using a natural or an artificial chemical doubling method for doubling the haploid plant and inbreeding; and 5)transgene DH line identification: performing a PCR level detection on the obtained DH line, and confirming the transgene maize inbred line containing the target gene. The method greatly shortens a cultivation process of the transgene maize inbred line.

The invention relates to a method for culturing a rice strain with the medium rice amylose content through the molecule label auxiliary selection. The method comprises the following steps: taking a K81 rice strain as a donor parent strain, taking a rice breed or strain with excellent agronomic characters and non-medium amylose content as a receptor parent strain, and carrying out the hybridization of the strains; after the hybridization, carrying out 2 to 3 times of backcross and then carrying out 3 to 5 times of inbreeding; and simultaneously planting the donor parent strain and the receptor parent strain for every generation. During the selection of the generations, a molecule label RA19 is used for auxiliary selection; a current generation individual strain containing a Wx gene of the donor parent strain is selected as the individual strain for the next backcross or inbreeding; and in addition, during the second selection and the later selection of inbred plant, the amylose content is determined for the selection. The method can be used to rapidly and effectively select the rice material with the medium amylose content, reduce the breeding scale and the breeding cost, improve the rice amylose content with high efficiency, and rapidly culture the rice strain with the medium rice amylose content.

The present invention relates to a male and female mating propagation method of tilapia, in specific to a mating propagation of tilapia which can adopt the pattern of male mating female; the method belongs to the technical field of aquatic product breeding. The method is characterized in that a net cage is equipped in an outdoor pond or indoor concrete pond; adult male tilapias are selected and people cut the upper jaw of the tilapias and put the tilapias into the net cage after being sterilized; and then adult female tilapias are selected to put in the net cage for mating culture and people can see the zygotes in the mouths of female tilapias after 7 to 10 days. With the present invention, a tilapia family line can be established for the family breeding of tilapias. In addition, people can clearly know the relation between the offspring and parent to control the inbreeding and avoid the germ plasm degeneration of offspring.

The invention relates to a preparation method for maize hybrids, belonging to the technical field of agricultural new species breeding. By taking MW94 line 449 as a female parent and ETO germ plasm as a male parent, a single hybrid is obtained through hybridization. By taking the single hybrid as a female parent and Luda red cob 340 as a male parent, a triple hybrid is obtained through hybridization. Superior plants are selected after two generations of continuous inbreeding. MW94 line 449 is used as a male parent for hybridization to obtain a base material. Then six generations of inbreeding are conducted to obtain an inbred line 216. The inbred line 216 of the invention has the advantages that a plurality of kinds of diseases such as gray speck can be resisted, the hereditary character is stable and the combining ability is strong, and is a superior inbred line for the preparation of maize hybrids.

The present invention relates to a method for the optimization of the fish variety, in particular to a method for the optimization of the carp variety, which resolves the problems that the inbreeding rate is high in the prior carp breeding cultivation and the intraspecific hybridization cultivation, thereby leading to the genetic degradation of the group and the decrease of the economic character and the production performance. The breeding procedures are: 1. performing the phenotypic character selection; 2. detecting the genetic information of the parent strain; 3. performing the reproduction grouping according to the genetic information; 4. the parent strains in each of the breeding group are free to mate with each other to get an optimized carp variety. The method of the present invention can avoid the inbreeding, so the variety can be optimized and the economic character and the production performance of the carps are obviously improved.

The invention relates to an optimization and purification method of small tail han sheep which comprises the steps that first, an appropriate number of first-grade stud rams and an appropriate number of first-grade ewes are selected to form a fundamental breeding herd, once the fundamental breeding herd is selected, breeding sheep are not introduced in all generations any more, and closed breeding is carried out; a certain number of G1 generation rams and G1 generation ewes are selected and remained from the next generation bred by the fundamental breeding herd; once the G1 generation ewes come into estrus, semen from one of G0 generation rams is randomly selected for mating, any degree of inbreeding is not limited; a certain number of small tail han sheep rams and small tail han sheep ewes are selected and remained from the G2 generation, the optimization and purification herd of the small tail han sheep is formed by the sheep obtained through the above steps and the sheep of the fundamental breeding herd. The optimization and purification method of small tail han sheep has the advantages that compared with the traditional methods, the small tail han sheep bred by the optimization and purification method have a faster genetic progress of germplasm characteristics between generations, and consistency of the population and seed performance are improved, and seed value is enhanced.

The invention provides a test case generation method based on a hierarchic genetic algorithm. The method can be used for effectively preventing and controlling inbreeding, so that the problems of premature convergence and local convergence are effectively solved. The method includes the steps that a program source code is inputted into a path analyzer, and the path analyzer carries out path analysis on the inputted program source code, then obtains a corresponding path list and outputs the path list to a test data generator; a predicate instrumenter carries out predicate instrumentation on the inputted source code and outputs the instrumented code to the test data generator; the test data generator firstly carries out hierarchy on an initial population through the hierarchic genetic algorithm, compares passing paths of individuals in hierarchies with objective paths of the individuals in the hierarchies through the utilization of the instrumented source code to obtain the matching values between the passing paths and the objective paths, and finally the test data generator gives test cases to the paths.

The invention discloses a breeding method of sterile and maintainer lines of north glutinous sorghum. The breeding method comprises the following specific steps: 1, hybridizing by taking non-glutinous sorghum LgB / R5M874B of a maintainer line as a female parent and a glutinous sorghum maintainer line 45B as a male parent, and inbreeding generations by selecting single plants with three good characters of glutinousness, large spikes and early mature; 2, hybridizing by taking a non-glutinous sorghum sterile line A2V4A as a female parent and an F2 generation in the step one as a male parent, carrying out four-generation backcross transformation by adopting a method of carrying out stable hybridization together with backcross transformation and taking good single plants of a filial generation as a male parent, and breeding a glutinous sorghum sterile line by taking good sterility, glutinous grains and heat smut resistance as breeding standards, wherein in glutinousness identification in the step one or step two, only endosperm is cut for carrying out iodine solution reaction, so that normal sprouting of the glutinous grains is ensured, and embryo is prevented from being damaged due to length cutting of the grains. According to the breeding method disclosed by the invention, good characters of yield, quality, resistance, combining ability, appearance and the like of south and north sorghum are organically integrated, so that the sterile and maintainer lines can become basic parent lines of the north glutinous sorghum, and the germplasm resource of the glutinous sorghum is enriched.

The invention relates to an identity card coding method for global pets and other artificial fed animals. The identity card coding method comprises the following steps of: determining national codes according to birthplaces of pets or residence places of pet feeders; determining regional codes inside one state; determining a postal service delivery region code; determining birth date codes according to birth times of the pets; determining digital sequence codes of the pets; determining gender codes of the pets; determining category codes of the pets; determining species codes of the pets; and sequentially combining the codes to generate identity codes of the pets and other artificially fed animals. According to the coding scheme designed by the invention, nations, regions in the state, postal service delivery regions, birth dates, numerical codes, genders, category, species and other information can be easily distinguished from the codes. Identity codes of the pets in different states and different species are unified. According to the codes, outbreeding and inbreeding of similar pets can be effectively avoided.

The invention discloses an inbreeding coefficient calculation model-based dairy cow hybridization method. The method comprises the following steps of: recording information of each dairy cow through abreeding bull electronic document database and a breeding cow electronic document database, wherein a database table structure comprises detailed information such as breeding bull numbers, breeding bull names, fathers, mothers, families, strains, dates of birth, places of birth, bodily forms, TPI and performance indexes; and when pairing is started, implanting the numbers of to-be-paired dairy cows and the numbers of sperm freezing bulls by an information system, automatically pairing and combining the sperm freezing parameters of each cow and each bull one by one by a computer system, reading a family tree relationship of the paired dairy cows in each group of pairing, constructing an electronic family tree graph, and processing data by utilizing an inbreeding coefficient calculation model so as to obtain an inbreeding coefficient of the combination. According to the method, systematic processing of large data volume can be rapidly and correctly carried out, so that unnecessary humanlabor can be greatly decreased, the correctness of calculated values can be ensured and the processing speed can be improved.

The invention discloses a method for breeding a sorghum sterile line, the method comprising: using a maintainer line and a restorer line to cross to breed a sterile line; the method comprises using the maintainer line QL33B as the female parent, and the restorer line Hui 13 is the male parent, crossed by artificial emasculation, and self-crossed continuously for 2 generations. In F3, a single plant with good comprehensive traits such as short plant height, compact plant type, and early growth period was selected to backcross with QL33B, and then continuously self-crossed for 3 In BC1F4, a single plant with short plant height, compact plant type and early growth period was selected to be backcrossed with QL33A to produce a sterile line. After 6 generations of continuous backcrossing, a stable sterile line Ji 2060A was transformed. The Ji 2060A bred by using the method for breeding a sorghum male sterile line provided by the present invention has a short growth period, a short plant, a large degree of secret planting that can be achieved by the plant, and a high yield per mu. The method for breeding sorghum sterile lines provided by the invention broadens the blood relationship of the existing sterile lines and improves the adaptability of the sterile lines.

The invention relates to a breeding method of masu salmons, relating to a breeding method of fishes. The method solves the technical problem that existing introduced masu salmons are featured with genetic drift, weak resistance, germplasm differentiation and quality degradation caused by small group and inbreeding. The method comprises the following steps: 1, establishing an F1-generation family; 2, cultivating the F1-generation family; 3, constructing a self-reproducing system in an F2-generation family and an F2-generation group; 4, breeding the F2-generation group by a three-stage elimination method; 5, constructing an F3-generation group and breeding by the three-stage elimination method; 6, constructing an F4-generation group and breeding by the three-stage elimination method; 7, constructing an F5-generation group and breeding by the three-stage elimination method; and 8, constructing an F6-generation group and breeding by the three-stage elimination method to finish breeding the masu salmons. The masu salmons obtained by using the method are featured with full body color, high growth speed and high postnatal survival rate.

The invention belongs to the technical field of breeding of vegetable crops, and discloses a selective breeding method of a male sterile line of Capsicum annuum var.frutescens L.. The selective breeding comprises the following steps: using an own common pepper maintenance line material as a female parent, selecting a single plant having a target character as a male parent from introduced three-line hybrid separation offspring having the target character and carrying out hybridization, and then carrying out inbreeding on the offspring to obtain hybridized F2; performing offspring fertility identification on a pre-selected maintenance line material (F2), selecting a plant line sterile plant of which a sterile plant ratio is 100 percent as the female parent, and using the single plant havingthe target character in the corresponding male parent plant line as the male parent and carrying out single-plant paired backcross, and simultaneously carrying out male parent inbreeding; carrying outbackcross and transformed breeding on the sterile line by utilizing a nuclear substitutional technology. The selective breeding method of the male sterile line of the Capsicum annuum var.frutescens L. disclosed by the invention is strong in complementarity of the characters and is wide in selection ranges; materials are easy to get, and the utilization rate of variety resources is improved; the method is simple, the controllability is strong and the reproduction rate is high; the bred sterile line is good in stability, high in combining ability, and is insensitive to temperature and illumination; moreover, the sterile plant rate and the sterility degree are both up to 100 percent.

The invention relates to an artificial propagation method of pangasias sutchi. The artificial propagation method comprises the following steps of selecting of parent fishes, culturing of parent fishes, artificial spawning induction, and artificial insemination; the zygotes are placed into a hatching tank to hatch for 50 to 60h in hatching water with temperature of 28 to 30 DEG C, and content of dissolved oxygen of 4mg / L. The artificial propagation method has the advantages that the fully-artificial hatching of the fishes can be realized, the condition of the parent fishes swallowing the fish fries is prohibited, the family line of the fishes can be established, the subsequent breeding of the family line is convenient, the relationship between the offsprings and the parents can be clearly known, the inbreeding can be controlled, and the genetical characterization decline of the offsprings produced by the inbreeding can be avoided.

The invention discloses a breeding method of a rapid growth strain of long mirror carps. By comprehensively utilizing genetic marker information, individual quantitative trait information, genetic distance and the like, through linkage analysis, parental combination is selected, so that the production performance and the accuracy can be improved, inbreeding is avoided, and the breeding progress isshortened. The breeding method comprises the following steps that individuals which are located among different reproduction pedigrees and parental individuals and has the genetic distance between 0.5 and 0.7 are selected, family or small group combination reproduction is performed, and an offspring reaches sexual maturity, namely breeding is finished, wherein the offspring of the individuals canenrich more than 6 preponderant alleles. The method provided by the invention can maintain relatively high genetic diversity of the mirror carps, improves the production performance of the mirror carps and accelerates the breeding progress of the mirror carps.

The invention relates to a water bird individual breeding method, which is characterized in that a bird house is divided into a plurality of independent small pens, each small pen comprises an inside playground and an outside playground, a plurality of egg laying nests are arranged inside the poultry house, the outside playground is divided into a continental playground and a water playground, and one male bird and a plurality of female birds are raised in each small pen; the female birds in the small pen are marked in a painting way in different colors, egg touching is carried out for each female bird before laying the egg every day, the female bird with the egg is closed into the corresponding egg laying nest and released after the egg laying, a number and a date of the female bird and the corresponding male bird are marked on each egg, all eggs laid by the same female bird are hatched together, and the fertility rate and hatching rate of each female bird are counted. Due to the adoption of the method, the egg laying situation of each female bird can be accurately recorded, father and mother of each young bird can be accurately recorded, the inbreeding can be effectively avoided, the breeding of the individual water bird can be reasonably optimized, the breeding purpose can be realized, and the water bird breeding quality and yield can be improved.

This invention provides a kind of mouse immunity state test reagent, this reagent is PBS suspension of mouse spleen cell in two inbreeding system which is stained by CFSE, the total concentration of the two kind of mouse spleen cell is 0.5-1 multiply 10 to the power 8 per ml, the proportion is 1:1, fluorescence tinction differential concentration is 1:20. Mainline the reagent in the model mouse; it can judge the immunity state of the model mouse by the change of the proportion of the two cell in this reagent when letting blood to test.

The invention discloses a hybrid breeding method for pumpkin by annual three generations, which is characterized in that: combinations are configured in a breeding field in spring of the first year, the combinations are preliminarily screened in the breeding field in autumn, and the enlargement and small-area seed production are performed on selected excellent combined parents in Hainan province in winder; the combining ability is determined in the breeding field in spring of the next year, variety comparison experiments are protectively performed in winter, and the enlargement and small-areaseed production of pioneer combined parents are continued in Hainan province in winder; the variety comparison experiments are continued in spring and autumn of the third year so as to select the optimal hybrid; the female parent is an inbred line 02-8A bred by performing seven generations of homozygous inbreeding on a hybrid winter jade according to an inbred line breeding method by annual threegenerations; and the male parent is an early-matured inbred line with a code number of 9601-4B obtained by hybridizing stable green-peel inbred line (with the code number of SQ96) selected by early-matured inbreeding separation in Seoul in spring in 2000 with a Chinese resource Heilongjiang small white-peel inbred line (with the code number of B) and performing four generations of homozygous inbreeding according to a female parent breeding method.

The invention relates to a method to improve breeding in dioecious plants, preferably Asparagus plants, comprising providing a plant in which the functional expression of the dominant suppressor of gynoecium development is disrupted or reduced and introducing said plant in inbreeding, backcross breeding, recurrent backcross breeding or double haploid breeding techniques. Preferably said dominant suppressor of gynoecium development is a gene comprising a DUF247 domain. Also provided are dioeciuos plants in which the expression of this gene is disrupted or reduced.

The invention discloses a breeding method of insect-resistant upland long stapled cotton. By adopting CRICAAS (Cotton Research Institute of Chinese Academy of Agricultural Sciences) No. 12 as a female parent and Darwinii cotton as a male parent, hybridization is carried out; F1 hybrid is used as a female parent, backcross on the F1 hybrid and the CRICAAS No. 12 is carried out for three times to obtain BC3F1; by using the BC3F1 as the female parent, hybridization on the BC3F1 and parental L41 of Xiangzamian No. 3 is carried out to obtain hybrid generation 1 used as a female parent, modification backcross on the hybrid generation 1 and the BC3F1 is carried out, and the backcross is carried out for four times to obtain BC4F1; then by using the BC4F1 as a female parent, the hybridization on the BC4F1 and Xinhai No. 5 is carried out, the backcross on the obtained hybrid generation 1 used as the female parent and the BC4F1 is carried out for four times; cotton-sealing inbreeding is carried out for six times, and separation is produced; finally, the insect-resistant long stapled cotton Xiang C176 is obtained. The yield of the Xiang C176 obtained by the invention is far higher than that of sea island cotton, and the insect-resistant upland long stapled cotton can be demonstrated and popularized in national main cotton planting areas, thereby increasing the self-supply ability of the long stapled cotton in China, and increasing the market competitiveness of high-grade raw cotton.